Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_A23
(934 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_56628| Best HMM Match : Actin (HMM E-Value=0) 247 1e-65
SB_7187| Best HMM Match : No HMM Matches (HMM E-Value=.) 242 2e-64
SB_23733| Best HMM Match : No HMM Matches (HMM E-Value=.) 239 2e-63
SB_7190| Best HMM Match : No HMM Matches (HMM E-Value=.) 239 2e-63
SB_56| Best HMM Match : Actin (HMM E-Value=0) 238 5e-63
SB_23734| Best HMM Match : No HMM Matches (HMM E-Value=.) 182 5e-46
SB_19204| Best HMM Match : No HMM Matches (HMM E-Value=.) 101 6e-22
SB_54| Best HMM Match : Actin (HMM E-Value=0) 64 1e-10
SB_3885| Best HMM Match : Actin (HMM E-Value=0.77) 64 2e-10
SB_27656| Best HMM Match : Actin (HMM E-Value=4.79999e-40) 61 1e-09
SB_34957| Best HMM Match : PARP (HMM E-Value=4.4e-12) 59 4e-09
SB_43920| Best HMM Match : SRP-alpha_N (HMM E-Value=4.6) 56 5e-08
SB_49385| Best HMM Match : Actin (HMM E-Value=0.00022) 55 9e-08
SB_3433| Best HMM Match : Actin (HMM E-Value=0.00011) 48 1e-05
SB_6996| Best HMM Match : Actin (HMM E-Value=1.7e-07) 40 0.002
SB_13343| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 5.4
>SB_56628| Best HMM Match : Actin (HMM E-Value=0)
Length = 376
Score = 247 bits (604), Expect = 1e-65
Identities = 111/117 (94%), Positives = 112/117 (95%)
Frame = +2
Query: 182 MCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAH 361
MCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEA
Sbjct: 1 MCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 60
Query: 362 XKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLXPKXXR 532
KRGILTLKYPIEHGI+TNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPL PK R
Sbjct: 61 SKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 117
Score = 72.9 bits (171), Expect = 3e-13
Identities = 35/44 (79%), Positives = 37/44 (84%)
Frame = +3
Query: 534 EKMXQIMFEXFNSPXMYVXIQAVLSLYASGRTXGIVLXXGDGVS 665
EKM QIMFE FNSP MYV IQAVLSLYASGRT GIV+ GDGV+
Sbjct: 118 EKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVMDSGDGVT 161
>SB_7187| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 349
Score = 242 bits (593), Expect = 2e-64
Identities = 108/117 (92%), Positives = 111/117 (94%)
Frame = +2
Query: 182 MCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAH 361
M DDD+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEA
Sbjct: 1 MADDDIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 60
Query: 362 XKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLXPKXXR 532
KRGILTLKYPIEHGI+TNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPL PK R
Sbjct: 61 SKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 117
Score = 74.1 bits (174), Expect = 1e-13
Identities = 36/44 (81%), Positives = 37/44 (84%)
Frame = +3
Query: 534 EKMXQIMFEXFNSPXMYVXIQAVLSLYASGRTXGIVLXXGDGVS 665
EKM QIMFE FNSP MYV IQAVLSLYASGRT GIV+ GDGVS
Sbjct: 118 EKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVMDSGDGVS 161
>SB_23733| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 376
Score = 239 bits (586), Expect = 2e-63
Identities = 108/117 (92%), Positives = 111/117 (94%)
Frame = +2
Query: 182 MCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAH 361
M DD++AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEA
Sbjct: 1 MEDDEIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQ 60
Query: 362 XKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLXPKXXR 532
KRGILTLKYPIEHGI+TNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPL PK R
Sbjct: 61 SKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 117
Score = 72.9 bits (171), Expect = 3e-13
Identities = 35/44 (79%), Positives = 37/44 (84%)
Frame = +3
Query: 534 EKMXQIMFEXFNSPXMYVXIQAVLSLYASGRTXGIVLXXGDGVS 665
EKM QIMFE FNSP MYV IQAVLSLYASGRT GIV+ GDGV+
Sbjct: 118 EKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVMDSGDGVT 161
>SB_7190| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 375
Score = 239 bits (586), Expect = 2e-63
Identities = 108/115 (93%), Positives = 110/115 (95%)
Frame = +2
Query: 188 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAHXK 367
+DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEA K
Sbjct: 2 EDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 61
Query: 368 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLXPKXXR 532
RGILTLKYPIEHGI+TNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPL PK R
Sbjct: 62 RGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 116
Score = 72.9 bits (171), Expect = 3e-13
Identities = 35/44 (79%), Positives = 37/44 (84%)
Frame = +3
Query: 534 EKMXQIMFEXFNSPXMYVXIQAVLSLYASGRTXGIVLXXGDGVS 665
EKM QIMFE FNSP MYV IQAVLSLYASGRT GIV+ GDGV+
Sbjct: 117 EKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVMDSGDGVT 160
>SB_56| Best HMM Match : Actin (HMM E-Value=0)
Length = 375
Score = 238 bits (582), Expect = 5e-63
Identities = 107/115 (93%), Positives = 110/115 (95%)
Frame = +2
Query: 188 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAHXK 367
+D+VAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEA K
Sbjct: 2 EDEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 61
Query: 368 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLXPKXXR 532
RGILTLKYPIEHGI+TNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPL PK R
Sbjct: 62 RGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 116
Score = 72.9 bits (171), Expect = 3e-13
Identities = 35/44 (79%), Positives = 37/44 (84%)
Frame = +3
Query: 534 EKMXQIMFEXFNSPXMYVXIQAVLSLYASGRTXGIVLXXGDGVS 665
EKM QIMFE FNSP MYV IQAVLSLYASGRT GIV+ GDGV+
Sbjct: 117 EKMTQIMFETFNSPAMYVAIQAVLSLYASGRTTGIVMDSGDGVT 160
>SB_23734| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 338
Score = 182 bits (442), Expect = 5e-46
Identities = 82/89 (92%), Positives = 84/89 (94%)
Frame = +2
Query: 188 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAHXK 367
DDDVAALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEA K
Sbjct: 2 DDDVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 61
Query: 368 RGILTLKYPIEHGIITNWDDMEKIWHHTF 454
RGILTLKYPIEHGI+TNWDDMEKI TF
Sbjct: 62 RGILTLKYPIEHGIVTNWDDMEKIMFETF 90
Score = 67.7 bits (158), Expect = 1e-11
Identities = 32/44 (72%), Positives = 36/44 (81%)
Frame = +3
Query: 534 EKMXQIMFEXFNSPXMYVXIQAVLSLYASGRTXGIVLXXGDGVS 665
+ M +IMFE FNSP MYV IQAVLSLYASGRT GIV+ GDGV+
Sbjct: 80 DDMEKIMFETFNSPAMYVAIQAVLSLYASGRTTGIVMDSGDGVT 123
>SB_19204| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 411
Score = 101 bits (243), Expect = 6e-22
Identities = 49/114 (42%), Positives = 71/114 (62%), Gaps = 3/114 (2%)
Frame = +2
Query: 200 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAHXKRG 373
+ +V DNG+G K G+AG + P +FPS+VGRP R + + KD VGDEA R
Sbjct: 7 SVIVCDNGTGFVKCGYAGSNFPAHIFPSMVGRPIIRSSQKVGDIEVKDLMVGDEASQLRY 66
Query: 374 ILTLKYPIEHGIITNWDDMEKIWHHTF-YNELRVAPEEHPVLLTEAPLXPKXXR 532
+L + YP+++GI+ NWDDM+ +W +TF +++ + P VLLTE PL P R
Sbjct: 67 MLEVNYPMDNGIVRNWDDMKHVWDYTFGESKMNIDPRNTKVLLTEPPLNPMKNR 120
Score = 51.6 bits (118), Expect = 9e-07
Identities = 22/44 (50%), Positives = 31/44 (70%)
Frame = +3
Query: 534 EKMXQIMFEXFNSPXMYVXIQAVLSLYASGRTXGIVLXXGDGVS 665
EKM ++MFE + +Y+ IQAVL+LYA G G+V+ GDGV+
Sbjct: 121 EKMIEVMFENYQFEGVYIAIQAVLTLYAQGLLTGVVIDSGDGVT 164
>SB_54| Best HMM Match : Actin (HMM E-Value=0)
Length = 2486
Score = 64.5 bits (150), Expect = 1e-10
Identities = 29/44 (65%), Positives = 34/44 (77%)
Frame = +3
Query: 534 EKMXQIMFEXFNSPXMYVXIQAVLSLYASGRTXGIVLXXGDGVS 665
E+M Q+MFE FN P MYV +QAV++LYASGRT G V GDGVS
Sbjct: 2162 ERMVQLMFESFNVPCMYVAVQAVMALYASGRTTGTVFDCGDGVS 2205
Score = 59.7 bits (138), Expect = 3e-09
Identities = 23/34 (67%), Positives = 30/34 (88%)
Frame = +2
Query: 206 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 307
+V+DNGSG CKAG + D++PR VFP+IVGRPRH+
Sbjct: 2097 VVIDNGSGFCKAGLSTDESPRVVFPAIVGRPRHK 2130
Score = 43.2 bits (97), Expect = 3e-04
Identities = 20/33 (60%), Positives = 24/33 (72%)
Frame = +2
Query: 434 KIWHHTFYNELRVAPEEHPVLLTEAPLXPKXXR 532
K+W H F ++LRV E+ PVLLTEAPL PK R
Sbjct: 2130 KMWEHAF-DQLRVRGEDFPVLLTEAPLNPKMNR 2161
>SB_3885| Best HMM Match : Actin (HMM E-Value=0.77)
Length = 152
Score = 63.7 bits (148), Expect = 2e-10
Identities = 30/39 (76%), Positives = 32/39 (82%)
Frame = +3
Query: 549 IMFEXFNSPXMYVXIQAVLSLYASGRTXGIVLXXGDGVS 665
IMFE FNSP +YV IQAVLSLYASGRT G+V GDGVS
Sbjct: 1 IMFEAFNSPAVYVAIQAVLSLYASGRTTGVVFDSGDGVS 39
>SB_27656| Best HMM Match : Actin (HMM E-Value=4.79999e-40)
Length = 367
Score = 60.9 bits (141), Expect = 1e-09
Identities = 27/48 (56%), Positives = 35/48 (72%)
Frame = +3
Query: 522 RXXGEKMXQIMFEXFNSPXMYVXIQAVLSLYASGRTXGIVLXXGDGVS 665
R EK ++ FE FN P +++ +QAVLSLYA+GRT G+VL GDGVS
Sbjct: 139 RRNREKAAEVFFETFNVPALFISMQAVLSLYATGRTTGVVLDAGDGVS 186
Score = 30.3 bits (65), Expect = 2.3
Identities = 12/17 (70%), Positives = 14/17 (82%)
Frame = +2
Query: 482 EHPVLLTEAPLXPKXXR 532
+HPVLLTEAPL P+ R
Sbjct: 126 QHPVLLTEAPLNPRRNR 142
>SB_34957| Best HMM Match : PARP (HMM E-Value=4.4e-12)
Length = 1392
Score = 59.3 bits (137), Expect = 4e-09
Identities = 26/34 (76%), Positives = 28/34 (82%)
Frame = +2
Query: 206 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 307
LV+D GS M K GFAGDDAP+ VFP IVGRPRHQ
Sbjct: 1359 LVIDVGSHMWKVGFAGDDAPKGVFPPIVGRPRHQ 1392
>SB_43920| Best HMM Match : SRP-alpha_N (HMM E-Value=4.6)
Length = 372
Score = 55.6 bits (128), Expect = 5e-08
Identities = 25/60 (41%), Positives = 34/60 (56%)
Frame = +2
Query: 320 GMGQKDSYVGDEAHXKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLL 499
G+ D ++GDEA K T K+PI H I+ +WD ME+ W + LR PE+H LL
Sbjct: 278 GVEDLDFFIGDEAIDKPSYAT-KWPIRHAIVEDWDLMERFWEQCIFKYLRAEPEDHYFLL 336
>SB_49385| Best HMM Match : Actin (HMM E-Value=0.00022)
Length = 921
Score = 54.8 bits (126), Expect = 9e-08
Identities = 29/73 (39%), Positives = 43/73 (58%), Gaps = 3/73 (4%)
Frame = +2
Query: 176 FKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKD-SYV 346
FK+ A+V+DNG G+ K GFAGD PR + P++VG P+ +++G + KD
Sbjct: 693 FKLDSHYNRAIVLDNGCGISKIGFAGDRVPRIIQPAVVGNPQRFSMLIGDNVNVKDYKKY 752
Query: 347 GDEAHXKRGILTL 385
GD A K G++ L
Sbjct: 753 GDAAVAKSGVMHL 765
>SB_3433| Best HMM Match : Actin (HMM E-Value=0.00011)
Length = 580
Score = 47.6 bits (108), Expect = 1e-05
Identities = 27/91 (29%), Positives = 49/91 (53%), Gaps = 2/91 (2%)
Frame = +2
Query: 197 VAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAHXKRGI 376
+A LV+D G+ K GFA + APR++ P+ + + + + ++ ++GD+ +
Sbjct: 233 MATLVLDCGACSQKIGFASNQAPRSI-PNAIFKAKSE-------RRKLFIGDQIEECKDY 284
Query: 377 LTLKY--PIEHGIITNWDDMEKIWHHTFYNE 463
L Y P + G + NWD ++IW +TF E
Sbjct: 285 SALFYVLPFQKGFLVNWDVEKQIWDYTFGKE 315
>SB_6996| Best HMM Match : Actin (HMM E-Value=1.7e-07)
Length = 240
Score = 40.3 bits (90), Expect = 0.002
Identities = 16/44 (36%), Positives = 29/44 (65%)
Frame = +3
Query: 522 RXXGEKMXQIMFEXFNSPXMYVXIQAVLSLYASGRTXGIVLXXG 653
R EK+ ++MFE +N P ++ +VL+ +A+GR+ G+V+ G
Sbjct: 46 RIKREKLTELMFEKYNVPAFFLCKNSVLTAFANGRSNGLVIDSG 89
Score = 34.3 bits (75), Expect = 0.14
Identities = 14/39 (35%), Positives = 24/39 (61%)
Frame = +2
Query: 392 PIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEA 508
PI+ G+I +WD EKI + + ++ HP+L++EA
Sbjct: 3 PIKDGMIEDWDLFEKILDYIYSKNIKSDSALHPLLMSEA 41
>SB_13343| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 198
Score = 29.1 bits (62), Expect = 5.4
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = -1
Query: 304 VARATHDXXXXXXXXXXXXETGLAHAGAIVDYKRSNIVV 188
+AR+ D ETGLAH GAI+D + +I+V
Sbjct: 1 MARSADDGGENGSGSIVSGETGLAHTGAIIDNQSCDIIV 39
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,693,645
Number of Sequences: 59808
Number of extensions: 280660
Number of successful extensions: 608
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 509
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 605
length of database: 16,821,457
effective HSP length: 82
effective length of database: 11,917,201
effective search space used: 2717121828
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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