Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP04_F_A08
         (875 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    30   0.38 
SPAC343.04c |gnr1||heterotrimeric G protein beta subunit Gnr1|Sc...    29   0.66 
SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha sub...    26   8.1  
SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosac...    26   8.1  

>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 800

 Score = 30.3 bits (65), Expect = 0.38
 Identities = 20/66 (30%), Positives = 28/66 (42%), Gaps = 1/66 (1%)
 Frame = +1

Query: 505 VTPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*SSITRKVLXMTVSSTV-IAPXTP 681
           VTP   P   S  S PP   +T  T  S  P  +++ S  T   +  T +ST  + P  P
Sbjct: 355 VTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLLSTSTSCTTSTSIPPTGNSTTPVTPTVP 414

Query: 682 SNTXGT 699
             +  T
Sbjct: 415 PTSSST 420



 Score = 29.1 bits (62), Expect = 0.87
 Identities = 20/60 (33%), Positives = 27/60 (45%)
 Frame = +1

Query: 505 VTPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*SSITRKVLXMTVSSTVIAPXTPS 684
           VTP   P   S  S PP   +T  T  S  P  +++ S  T   +  T +ST   P TP+
Sbjct: 301 VTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLPSTSTSCTTSTSIPPTGNST--TPVTPT 358



 Score = 28.3 bits (60), Expect = 1.5
 Identities = 20/61 (32%), Positives = 28/61 (45%), Gaps = 1/61 (1%)
 Frame = +1

Query: 505 VTPKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLXMTVSSTVIAPXTP 681
           +TP   P   S  S+P P   T+   + S P  T ST S  T   +    SS++  P TP
Sbjct: 191 ITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTST-SCTTSTSIPTGGSSSLSTPITP 249

Query: 682 S 684
           +
Sbjct: 250 T 250



 Score = 27.5 bits (58), Expect = 2.7
 Identities = 17/60 (28%), Positives = 26/60 (43%), Gaps = 1/60 (1%)
 Frame = +1

Query: 505 VTPKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLXMTVSSTVIAPXTP 681
           +TP   P   S  S+P P   T+   + S P  T ST  + +  +     S+T + P  P
Sbjct: 247 ITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTSTSCTTSTSIPPTGNSTTPVTPTVP 306



 Score = 25.8 bits (54), Expect = 8.1
 Identities = 20/69 (28%), Positives = 29/69 (42%), Gaps = 1/69 (1%)
 Frame = +1

Query: 496 LHSVTPKTKPARKSPGSLPPCW-KTTEFTSRSCPPRTNST*SSITRKVLXMTVSSTVIAP 672
           L++ TP T P   S  S    W  TT  T  S    T+ST   +T      T  ++  + 
Sbjct: 598 LYTSTPITSPNSTSSSSTQVSWNSTTPITGTSTSKVTSSTSIPLTSTNRTSTTFTSSTSI 657

Query: 673 XTPSNTXGT 699
            T S++  T
Sbjct: 658 STSSSSTAT 666


>SPAC343.04c |gnr1||heterotrimeric G protein beta subunit
           Gnr1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 507

 Score = 29.5 bits (63), Expect = 0.66
 Identities = 15/34 (44%), Positives = 20/34 (58%)
 Frame = -1

Query: 587 DLEVNSVVFQHGGKLPGDFLAGFVFGVTECNFVV 486
           DLE N +V Q+ G   G+FL G  FG  +  FV+
Sbjct: 411 DLEENRIVRQYMGHKLGNFLIGSCFGGKDDTFVL 444


>SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha
           subunit Pda1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 409

 Score = 25.8 bits (54), Expect = 8.1
 Identities = 11/26 (42%), Positives = 16/26 (61%)
 Frame = -1

Query: 719 FVHGGLKVPXVFEGVXGAITVDDTVI 642
           F H  +    V  G+ GAIT+DD++I
Sbjct: 107 FCHLSIGQEAVAAGIEGAITLDDSII 132


>SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit
           Smc6|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1140

 Score = 25.8 bits (54), Expect = 8.1
 Identities = 12/31 (38%), Positives = 17/31 (54%)
 Frame = +3

Query: 435 LINKRDXHALKLIDQQNHNKIAFGDSKDKTS 527
           L+ K+D  A K+ DQ  H K+     +DK S
Sbjct: 496 LLTKKDSIANKISDQSEHLKVLEDVQRDKVS 526


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,746,120
Number of Sequences: 5004
Number of extensions: 48397
Number of successful extensions: 161
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 158
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 438479610
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)