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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP04_F_A07
         (830 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr...   215   6e-57
SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|...   117   2e-27
SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces ...    42   1e-04
SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch...    41   2e-04
SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce...    34   0.028
SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe...    33   0.050
SPAC19D5.07 |uga1||4-aminobutyrate aminotransferase |Schizosacch...    25   10.0 

>SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 591

 Score =  215 bits (525), Expect = 6e-57
 Identities = 103/172 (59%), Positives = 114/172 (66%)
 Frame = +2

Query: 119 LXRVLNVXLPAPXXLXTPEANAFLNXLCNXXXLXCSPPRXXAXLLDKLVSVFLXXXCXNP 298
           L   LN   P    L TPEANAFL  LC    + C+PP+  + LLDKLV  F+   C NP
Sbjct: 404 LEEKLNTKFPPGDQLHTPEANAFLRDLCAKHGVECAPPQTCSRLLDKLVGEFIESECINP 463

Query: 299 XFXLXXPQXMXPLSKYHRXVPGLTXXFEVYVAKXXICNAYTELNDPATQREXFEEQAXNR 478
            F +  PQ M PL+KYHR   GL   FE +VA   ICNAYTELND   QR  FEEQA  +
Sbjct: 464 TFIIGHPQMMSPLAKYHRSDAGLCERFEAFVATKEICNAYTELNDIFDQRARFEEQARQK 523

Query: 479 AAGDDETPPTDEAFCTALEYGLPPTGGWGLGVDRLTMFLTDSNNIKEVLLFP 634
           A GDDE    DE FCTALEYGLPPTGGWG+GVDRL MFLTDSN I+EVLLFP
Sbjct: 524 AQGDDEAQIIDENFCTALEYGLPPTGGWGMGVDRLVMFLTDSNTIREVLLFP 575


>SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 531

 Score =  117 bits (281), Expect = 2e-27
 Identities = 63/137 (45%), Positives = 78/137 (56%), Gaps = 7/137 (5%)
 Frame = +2

Query: 242 AXLLDKLV-SVFLXXXCXNPXFXLXXPQXMXPLSK-----YHRXVPGLTXXFEVYVAKXX 403
           A LLDKL  S+ L     +P F +  P+ M PL+K     Y      ++  FE+Y+    
Sbjct: 389 AHLLDKLFDSLVLKYNTSSPKFVINHPEVMSPLAKSDIKLYGAVEQRISKRFELYIGGYE 448

Query: 404 ICNAYTELNDPATQREXFEEQAXNRAA-GDDETPPTDEAFCTALEYGLPPTGGWGLGVDR 580
           ICNAY E NDP  Q   F+ Q  +R   GDDETP  D  F  ALEYGLPPT GWG+GVDR
Sbjct: 449 ICNAYEEENDPVAQYHKFQAQKYDRLQLGDDETPAPDSDFVHALEYGLPPTAGWGMGVDR 508

Query: 581 LTMFLTDSNNIKEVLLF 631
           L M +T  + I E+L F
Sbjct: 509 LVMLMTGQSKISEILPF 525


>SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 441

 Score = 41.9 bits (94), Expect = 1e-04
 Identities = 19/46 (41%), Positives = 28/46 (60%), Gaps = 1/46 (2%)
 Frame = +2

Query: 500 PPTDEAFCTALEYGLPPTGGWGLGVDRLTMFLTDSN-NIKEVLLFP 634
           PP  + +   ++Y   P GG+GLG++RL  FL   N N+KE + FP
Sbjct: 388 PPELQWYLDLMKYSNAPHGGFGLGIERLIAFLEGENTNVKETIPFP 433


>SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 611

 Score = 41.1 bits (92), Expect = 2e-04
 Identities = 20/43 (46%), Positives = 24/43 (55%)
 Frame = +2

Query: 506 TDEAFCTALEYGLPPTGGWGLGVDRLTMFLTDSNNIKEVLLFP 634
           T E     L  G PP GG  LG DRL   LT++  I+EV+ FP
Sbjct: 533 TFEHLIRVLSSGCPPHGGIALGFDRLAALLTNAPGIREVIAFP 575


>SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 568

 Score = 33.9 bits (74), Expect = 0.028
 Identities = 15/39 (38%), Positives = 21/39 (53%)
 Frame = +2

Query: 518 FCTALEYGLPPTGGWGLGVDRLTMFLTDSNNIKEVLLFP 634
           F    +YG    GG GLG++R   +L D   ++E  LFP
Sbjct: 522 FTEQRKYGTTEHGGCGLGLERFLAWLCDRYTVRECCLFP 560


>SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 580

 Score = 33.1 bits (72), Expect = 0.050
 Identities = 14/41 (34%), Positives = 22/41 (53%)
 Frame = +2

Query: 512 EAFCTALEYGLPPTGGWGLGVDRLTMFLTDSNNIKEVLLFP 634
           + +  A   G PP  G G+G++R+ MF  +  NI+    FP
Sbjct: 532 QQYIDAFAIGCPPHAGGGIGLERVVMFYLNLPNIRLASSFP 572


>SPAC19D5.07 |uga1||4-aminobutyrate aminotransferase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 474

 Score = 25.4 bits (53), Expect = 10.0
 Identities = 15/54 (27%), Positives = 23/54 (42%)
 Frame = +2

Query: 482 AGDDETPPTDEAFCTALEYGLPPTGGWGLGVDRLTMFLTDSNNIKEVLLFPXNE 643
           A D E+P   + FC  +       GG G+   RL   L    +  ++LL   +E
Sbjct: 419 AWDCESPAARDKFCADMRINGVNIGGCGVAAIRLRPMLVFQKHHAQILLKKIDE 472


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,333,934
Number of Sequences: 5004
Number of extensions: 39271
Number of successful extensions: 83
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 79
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 81
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 408446760
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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