BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP04_F_A07 (830 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr... 215 6e-57 SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|... 117 2e-27 SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces ... 42 1e-04 SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch... 41 2e-04 SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyce... 34 0.028 SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe... 33 0.050 SPAC19D5.07 |uga1||4-aminobutyrate aminotransferase |Schizosacch... 25 10.0 >SPBC17G9.03c |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 2|||Manual Length = 591 Score = 215 bits (525), Expect = 6e-57 Identities = 103/172 (59%), Positives = 114/172 (66%) Frame = +2 Query: 119 LXRVLNVXLPAPXXLXTPEANAFLNXLCNXXXLXCSPPRXXAXLLDKLVSVFLXXXCXNP 298 L LN P L TPEANAFL LC + C+PP+ + LLDKLV F+ C NP Sbjct: 404 LEEKLNTKFPPGDQLHTPEANAFLRDLCAKHGVECAPPQTCSRLLDKLVGEFIESECINP 463 Query: 299 XFXLXXPQXMXPLSKYHRXVPGLTXXFEVYVAKXXICNAYTELNDPATQREXFEEQAXNR 478 F + PQ M PL+KYHR GL FE +VA ICNAYTELND QR FEEQA + Sbjct: 464 TFIIGHPQMMSPLAKYHRSDAGLCERFEAFVATKEICNAYTELNDIFDQRARFEEQARQK 523 Query: 479 AAGDDETPPTDEAFCTALEYGLPPTGGWGLGVDRLTMFLTDSNNIKEVLLFP 634 A GDDE DE FCTALEYGLPPTGGWG+GVDRL MFLTDSN I+EVLLFP Sbjct: 524 AQGDDEAQIIDENFCTALEYGLPPTGGWGMGVDRLVMFLTDSNTIREVLLFP 575 >SPCC18.08 |||lysine-tRNA ligase|Schizosaccharomyces pombe|chr 3|||Manual Length = 531 Score = 117 bits (281), Expect = 2e-27 Identities = 63/137 (45%), Positives = 78/137 (56%), Gaps = 7/137 (5%) Frame = +2 Query: 242 AXLLDKLV-SVFLXXXCXNPXFXLXXPQXMXPLSK-----YHRXVPGLTXXFEVYVAKXX 403 A LLDKL S+ L +P F + P+ M PL+K Y ++ FE+Y+ Sbjct: 389 AHLLDKLFDSLVLKYNTSSPKFVINHPEVMSPLAKSDIKLYGAVEQRISKRFELYIGGYE 448 Query: 404 ICNAYTELNDPATQREXFEEQAXNRAA-GDDETPPTDEAFCTALEYGLPPTGGWGLGVDR 580 ICNAY E NDP Q F+ Q +R GDDETP D F ALEYGLPPT GWG+GVDR Sbjct: 449 ICNAYEEENDPVAQYHKFQAQKYDRLQLGDDETPAPDSDFVHALEYGLPPTAGWGMGVDR 508 Query: 581 LTMFLTDSNNIKEVLLF 631 L M +T + I E+L F Sbjct: 509 LVMLMTGQSKISEILPF 525 >SPBC1198.10c |||asparagine-tRNA ligase Slm5|Schizosaccharomyces pombe|chr 2|||Manual Length = 441 Score = 41.9 bits (94), Expect = 1e-04 Identities = 19/46 (41%), Positives = 28/46 (60%), Gaps = 1/46 (2%) Frame = +2 Query: 500 PPTDEAFCTALEYGLPPTGGWGLGVDRLTMFLTDSN-NIKEVLLFP 634 PP + + ++Y P GG+GLG++RL FL N N+KE + FP Sbjct: 388 PPELQWYLDLMKYSNAPHGGFGLGIERLIAFLEGENTNVKETIPFP 433 >SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr 3|||Manual Length = 611 Score = 41.1 bits (92), Expect = 2e-04 Identities = 20/43 (46%), Positives = 24/43 (55%) Frame = +2 Query: 506 TDEAFCTALEYGLPPTGGWGLGVDRLTMFLTDSNNIKEVLLFP 634 T E L G PP GG LG DRL LT++ I+EV+ FP Sbjct: 533 TFEHLIRVLSSGCPPHGGIALGFDRLAALLTNAPGIREVIAFP 575 >SPBC1773.10c |||asparagine-tRNA ligase Ded81 |Schizosaccharomyces pombe|chr 2|||Manual Length = 568 Score = 33.9 bits (74), Expect = 0.028 Identities = 15/39 (38%), Positives = 21/39 (53%) Frame = +2 Query: 518 FCTALEYGLPPTGGWGLGVDRLTMFLTDSNNIKEVLLFP 634 F +YG GG GLG++R +L D ++E LFP Sbjct: 522 FTEQRKYGTTEHGGCGLGLERFLAWLCDRYTVRECCLFP 560 >SPCC1223.07c |||aspartate-tRNA ligase |Schizosaccharomyces pombe|chr 3|||Manual Length = 580 Score = 33.1 bits (72), Expect = 0.050 Identities = 14/41 (34%), Positives = 22/41 (53%) Frame = +2 Query: 512 EAFCTALEYGLPPTGGWGLGVDRLTMFLTDSNNIKEVLLFP 634 + + A G PP G G+G++R+ MF + NI+ FP Sbjct: 532 QQYIDAFAIGCPPHAGGGIGLERVVMFYLNLPNIRLASSFP 572 >SPAC19D5.07 |uga1||4-aminobutyrate aminotransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 474 Score = 25.4 bits (53), Expect = 10.0 Identities = 15/54 (27%), Positives = 23/54 (42%) Frame = +2 Query: 482 AGDDETPPTDEAFCTALEYGLPPTGGWGLGVDRLTMFLTDSNNIKEVLLFPXNE 643 A D E+P + FC + GG G+ RL L + ++LL +E Sbjct: 419 AWDCESPAARDKFCADMRINGVNIGGCGVAAIRLRPMLVFQKHHAQILLKKIDE 472 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,333,934 Number of Sequences: 5004 Number of extensions: 39271 Number of successful extensions: 83 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 79 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 81 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 408446760 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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