Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP04_F_A07
(830 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative / lysin... 195 3e-50
At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N) f... 98 5e-21
At4g26870.1 68417.m03867 aspartyl-tRNA synthetase, putative / as... 51 1e-06
At4g31180.2 68417.m04427 aspartyl-tRNA synthetase, putative / as... 40 0.002
At4g31180.1 68417.m04426 aspartyl-tRNA synthetase, putative / as... 40 0.002
At4g33760.1 68417.m04793 tRNA synthetase class II (D, K and N) f... 40 0.002
At4g17300.1 68417.m02598 asparaginyl-tRNA synthetase, chloroplas... 40 0.002
At5g56680.1 68418.m07075 asparaginyl-tRNA synthetase 1, cytoplas... 34 0.13
At1g70980.1 68414.m08188 asparaginyl-tRNA synthetase, cytoplasmi... 33 0.23
>At3g11710.1 68416.m01435 lysyl-tRNA synthetase, putative /
lysine--tRNA ligase, putative similar to SP|Q43776
Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--tRNA ligase)
{Lycopersicon esculentum}; contains Pfam profile
PF00152: tRNA synthetases class II (D, K and N)
Length = 626
Score = 195 bits (476), Expect = 3e-50
Identities = 90/164 (54%), Positives = 108/164 (65%)
Frame = +2
Query: 143 LPAPXXLXTPEANAFLNXLCNXXXLXCSPPRXXAXLLDKLVSVFLXXXCXNPXFXLXXPQ 322
L P L + EAN +L C + C PP+ A LLDKLV FL C NP F + P+
Sbjct: 442 LNIPKDLASEEANKYLIDACARFDVKCPPPQTTARLLDKLVGEFLEPTCVNPTFIINQPE 501
Query: 323 XMXPLSKYHRXVPGLTXXFEVYVAKXXICNAYTELNDPATQREXFEEQAXNRAAGDDETP 502
M PL+K+HR GLT FE+++ K +CNAYTELNDP QR+ F +Q +R +GDDE
Sbjct: 502 IMSPLAKWHRSKSGLTERFELFINKHELCNAYTELNDPVVQRQRFADQLKDRQSGDDEAM 561
Query: 503 PTDEAFCTALEYGLPPTGGWGLGVDRLTMFLTDSNNIKEVLLFP 634
DE FC ALEYGL PTGGWGLG+DRL+M LTDS NIKEVL FP
Sbjct: 562 ALDETFCNALEYGLAPTGGWGLGIDRLSMLLTDSLNIKEVLFFP 605
>At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N)
family protein similar to SP|Q9RHV9 Lysyl-tRNA
synthetase (EC 6.1.1.6) (Lysine--tRNA ligase) {Bacillus
stearothermophilus}; contains Pfam profile: PF00152 tRNA
synthetases class II (D, K and N)
Length = 602
Score = 98.3 bits (234), Expect = 5e-21
Identities = 58/149 (38%), Positives = 78/149 (52%), Gaps = 20/149 (13%)
Frame = +2
Query: 248 LLDKLVSVFLXXXCXNPXFXLXXPQXMXPLSKYHRXVPGLTXXFEVYVAKXXICNAYTEL 427
LL+++ V + P F L P + PL+K HR GLT FE+++ + NA++EL
Sbjct: 448 LLNEIFEVVVEPKLVQPTFVLDYPIEISPLAKPHRGNAGLTERFELFICGREMANAFSEL 507
Query: 428 NDPATQREXFEEQAXNRAA------------------GDDETPPT--DEAFCTALEYGLP 547
DP QR EEQ A DDE+ DE F TALEYG+P
Sbjct: 508 TDPVDQRTRLEEQVRQHNAKRAEAVRESPEPNAKKDDDDDESYEVTLDEDFLTALEYGMP 567
Query: 548 PTGGWGLGVDRLTMFLTDSNNIKEVLLFP 634
P G GLG+DRL M LT+S +I++V+ FP
Sbjct: 568 PASGMGLGIDRLVMLLTNSASIRDVIAFP 596
>At4g26870.1 68417.m03867 aspartyl-tRNA synthetase, putative /
aspartate--tRNA ligase, putative simialr to
aspartate-tRNA ligase (EC 6.1.1.12) from Drosophila
melanogaster GI:4512034, Homo sapiens SP|P14868, Rattus
norvegicus SP|P15178; contains Pfam profile PF00152 tRNA
synthetases class II (D, K and N)
Length = 532
Score = 50.8 bits (116), Expect = 1e-06
Identities = 30/86 (34%), Positives = 46/86 (53%)
Frame = +2
Query: 377 FEVYVAKXXICNAYTELNDPATQREXFEEQAXNRAAGDDETPPTDEAFCTALEYGLPPTG 556
F+V++ I + ++DP E E++A R G D T + A YG PP G
Sbjct: 447 FDVFIRGEEIMSGAQRIHDP----ELLEKRA--RECGIDVK--TISTYIDAFRYGAPPHG 498
Query: 557 GWGLGVDRLTMFLTDSNNIKEVLLFP 634
G+G+G++R+ M L NNI++ LFP
Sbjct: 499 GFGVGLERVVMLLCALNNIRKTSLFP 524
>At4g31180.2 68417.m04427 aspartyl-tRNA synthetase, putative /
aspartate--tRNA ligase, putative similar to
Aspartyl-tRNA synthetase (Aspartate--tRNA ligase)
(AspRS) [Homo sapiens] GI:20178330
Length = 558
Score = 40.3 bits (90), Expect = 0.002
Identities = 21/64 (32%), Positives = 30/64 (46%)
Frame = +2
Query: 443 QREXFEEQAXNRAAGDDETPPTDEAFCTALEYGLPPTGGWGLGVDRLTMFLTDSNNIKEV 622
QR E RA T + + YG P GG+G+G++R+ M NNI++
Sbjct: 487 QRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCALNNIRKT 546
Query: 623 LLFP 634
LFP
Sbjct: 547 SLFP 550
>At4g31180.1 68417.m04426 aspartyl-tRNA synthetase, putative /
aspartate--tRNA ligase, putative similar to
Aspartyl-tRNA synthetase (Aspartate--tRNA ligase)
(AspRS) [Homo sapiens] GI:20178330
Length = 558
Score = 40.3 bits (90), Expect = 0.002
Identities = 21/64 (32%), Positives = 30/64 (46%)
Frame = +2
Query: 443 QREXFEEQAXNRAAGDDETPPTDEAFCTALEYGLPPTGGWGLGVDRLTMFLTDSNNIKEV 622
QR E RA T + + YG P GG+G+G++R+ M NNI++
Sbjct: 487 QRVHIPEVLEQRAGECGIDVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCALNNIRKT 546
Query: 623 LLFP 634
LFP
Sbjct: 547 SLFP 550
>At4g33760.1 68417.m04793 tRNA synthetase class II (D, K and N)
family protein similar to SP|P36419 Aspartyl-tRNA
synthetase (EC 6.1.1.12) (Aspartate--tRNA ligase)
(AspRS) {Thermus thermophilus}; contains Pfam profile
PF00152: tRNA synthetases class II (D, K and N)
Length = 664
Score = 39.9 bits (89), Expect = 0.002
Identities = 16/36 (44%), Positives = 25/36 (69%)
Frame = +2
Query: 527 ALEYGLPPTGGWGLGVDRLTMFLTDSNNIKEVLLFP 634
AL+ G PP GG G+DR+ M L +++I++V+ FP
Sbjct: 600 ALDMGAPPHGGIAYGLDRMVMMLGGASSIRDVIAFP 635
>At4g17300.1 68417.m02598 asparaginyl-tRNA synthetase, chloroplast,
mitochondrial / asparagine-tRNA ligase / AsnRS (SYNO)
nearly identical to SP|O48593
Length = 567
Score = 39.9 bits (89), Expect = 0.002
Identities = 17/37 (45%), Positives = 24/37 (64%)
Frame = +2
Query: 536 YGLPPTGGWGLGVDRLTMFLTDSNNIKEVLLFPXNEA 646
YG P G+GLG +RL F+T +NI++V+ FP A
Sbjct: 527 YGSVPHAGFGLGFERLVQFVTGIDNIRDVIPFPRTPA 563
>At5g56680.1 68418.m07075 asparaginyl-tRNA synthetase 1, cytoplasmic
/ asparagine-tRNA ligase 1 (SYNC1) identical to
SP|Q9SW96
Length = 572
Score = 33.9 bits (74), Expect = 0.13
Identities = 14/33 (42%), Positives = 22/33 (66%)
Frame = +2
Query: 536 YGLPPTGGWGLGVDRLTMFLTDSNNIKEVLLFP 634
YG G+GLG +R+ +F T +NI++V+ FP
Sbjct: 532 YGTVKHCGFGLGFERMILFATGLDNIRDVIPFP 564
>At1g70980.1 68414.m08188 asparaginyl-tRNA synthetase, cytoplasmic,
putative / asparagine-tRNA ligase, putative similar to
SYNC1 protein GI:5670315 [SP|Q9SW96] from [Arabidopsis
thaliana]
Length = 571
Score = 33.1 bits (72), Expect = 0.23
Identities = 14/33 (42%), Positives = 21/33 (63%)
Frame = +2
Query: 536 YGLPPTGGWGLGVDRLTMFLTDSNNIKEVLLFP 634
YG G+GLG +R+ F T +NI++V+ FP
Sbjct: 531 YGTVKHCGFGLGFERMIQFATGIDNIRDVIPFP 563
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,782,035
Number of Sequences: 28952
Number of extensions: 226006
Number of successful extensions: 443
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 434
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 443
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1911862400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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