BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP04_F_A04 (867 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 316 3e-87 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 305 5e-84 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 280 2e-76 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 270 1e-73 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 223 3e-59 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 118 8e-28 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 82 1e-16 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 70 5e-13 SPBC18A7.01 ||SPBC4F6.19c|X-Pro dipeptidase |Schizosaccharomyces... 31 0.21 SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 1.1 SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 26 6.0 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 316 bits (776), Expect = 3e-87 Identities = 151/190 (79%), Positives = 168/190 (88%) Frame = +1 Query: 256 SYVAFTDTERLIGDAAKNXVAMNPNNTIFDAKRLIGRKFEDATVQADMKXWPFEVVSDGG 435 SYVAFTDTERLIGDAAKN VAMNP+NTIFDAKRLIGRKF+D VQ+DMK WPF+V+S G Sbjct: 38 SYVAFTDTERLIGDAAKNQVAMNPHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDG 97 Query: 436 XPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATK 615 P ++V YKGE KTF PEE+SSMVL KM+ETAEAYLG V +AV+TVPAYFNDSQRQATK Sbjct: 98 KPVLQVEYKGETKTFTPEEISSMVLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATK 157 Query: 616 DAGTISGLNVLRIINEPTAAAIAYGLDQKGTGXRHVLIFDLGGGTFDVSILTIEDGIFXV 795 DAG I+GLNVLRIINEPTAAAIAYGLD+ G +VLIFDLGGGTFDVS+LTIE+GIF V Sbjct: 158 DAGLIAGLNVLRIINEPTAAAIAYGLDRSNQGESNVLIFDLGGGTFDVSLLTIEEGIFEV 217 Query: 796 KSTAGDTHLG 825 K+TAGDTHLG Sbjct: 218 KATAGDTHLG 227 Score = 59.3 bits (137), Expect = 7e-10 Identities = 25/34 (73%), Positives = 29/34 (85%) Frame = +3 Query: 153 AVGIDLGTXYSCVGVFQHGKVEXIANDQGNRTTP 254 ++GIDLGT YSCVG F + +VE IANDQGNRTTP Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTP 37 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 305 bits (749), Expect = 5e-84 Identities = 146/190 (76%), Positives = 165/190 (86%) Frame = +1 Query: 256 SYVAFTDTERLIGDAAKNXVAMNPNNTIFDAKRLIGRKFEDATVQADMKXWPFEVVSDGG 435 SYVAFTDTERLIGDAAKN VAMNP+NTIFDAKRLIGR+F D VQ+DMK WPF+V+ G Sbjct: 38 SYVAFTDTERLIGDAAKNQVAMNPHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDG 97 Query: 436 XPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATK 615 P I+V +KGE KTF PEE+SSMVL KM+E+AEA+LG V +AV+TVPAYFNDSQRQATK Sbjct: 98 KPLIQVEFKGETKTFTPEEISSMVLLKMRESAEAFLGGKVTDAVVTVPAYFNDSQRQATK 157 Query: 616 DAGTISGLNVLRIINEPTAAAIAYGLDQKGTGXRHVLIFDLGGGTFDVSILTIEDGIFXV 795 DAG I+GLNVLRIINEPTAAAIAYGLD+ +VLIFDLGGGTFDVS+LTIE+GIF V Sbjct: 158 DAGLIAGLNVLRIINEPTAAAIAYGLDRSNQHETNVLIFDLGGGTFDVSLLTIEEGIFEV 217 Query: 796 KSTAGDTHLG 825 K+TAGDTHLG Sbjct: 218 KATAGDTHLG 227 Score = 59.3 bits (137), Expect = 7e-10 Identities = 25/34 (73%), Positives = 29/34 (85%) Frame = +3 Query: 153 AVGIDLGTXYSCVGVFQHGKVEXIANDQGNRTTP 254 ++GIDLGT YSCVG F + +VE IANDQGNRTTP Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTP 37 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 280 bits (686), Expect = 2e-76 Identities = 133/200 (66%), Positives = 158/200 (79%) Frame = +1 Query: 226 PTTRATGPLRSYVAFTDTERLIGDAAKNXVAMNPNNTIFDAKRLIGRKFEDATVQADMKX 405 P + S+VAFT+TERL+GDAAKN AMNP NT+FDAKRLIGR++ED Q D+K Sbjct: 32 PNDQGARTTPSFVAFTETERLVGDAAKNQAAMNPRNTVFDAKRLIGRRYEDPETQKDIKH 91 Query: 406 WPFEVVSDGGXPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAY 585 WPF+V+ + G P I+V Y GE K F +E+S+MVLTKMKE +EA L K V+ AVITVPAY Sbjct: 92 WPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAMVLTKMKEISEAKLNKRVEKAVITVPAY 151 Query: 586 FNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDQKGTGXRHVLIFDLGGGTFDVSI 765 F+DSQR ATKDAG I+GLNVLRIINEPTAAAIAYGLD K ++VLIFDLGGGTFDVS+ Sbjct: 152 FSDSQRAATKDAGAIAGLNVLRIINEPTAAAIAYGLDAKSDKPKNVLIFDLGGGTFDVSL 211 Query: 766 LTIEDGIFXVKSTAGDTHLG 825 L I+ G+F V +TAGDTHLG Sbjct: 212 LKIQGGVFEVLATAGDTHLG 231 Score = 52.4 bits (120), Expect = 8e-08 Identities = 23/34 (67%), Positives = 26/34 (76%) Frame = +3 Query: 153 AVGIDLGTXYSCVGVFQHGKVEXIANDQGNRTTP 254 A+GIDLGT YSCV V++ VE I NDQG RTTP Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTP 41 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 270 bits (663), Expect = 1e-73 Identities = 123/190 (64%), Positives = 160/190 (84%) Frame = +1 Query: 256 SYVAFTDTERLIGDAAKNXVAMNPNNTIFDAKRLIGRKFEDATVQADMKXWPFEVVSDGG 435 SYVAFT+ ERL+G+AAKN NP NTIFD KRLIGRKF++ T+ D+K +PF +V+D Sbjct: 71 SYVAFTEDERLVGEAAKNQAPSNPENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKN 130 Query: 436 XPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQATK 615 P ++V G+ K F PEE+S+M+L+KMK+TAEAYLGK V +AV+TVPAYFND+QRQATK Sbjct: 131 RPLVEVNVGGKKKKFTPEEISAMILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATK 190 Query: 616 DAGTISGLNVLRIINEPTAAAIAYGLDQKGTGXRHVLIFDLGGGTFDVSILTIEDGIFXV 795 DAGTI+GLNV+RI+NEPTAAAIAYGLD+ T +H++++DLGGGTFDVS+L+I++G+F V Sbjct: 191 DAGTIAGLNVIRIVNEPTAAAIAYGLDKTDT-EKHIVVYDLGGGTFDVSLLSIDNGVFEV 249 Query: 796 KSTAGDTHLG 825 +T+GDTHLG Sbjct: 250 LATSGDTHLG 259 Score = 57.2 bits (132), Expect = 3e-09 Identities = 24/33 (72%), Positives = 28/33 (84%) Frame = +3 Query: 156 VGIDLGTXYSCVGVFQHGKVEXIANDQGNRTTP 254 +GIDLGT YSCV V ++G+VE IANDQGNR TP Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITP 70 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 223 bits (545), Expect = 3e-59 Identities = 113/215 (52%), Positives = 148/215 (68%), Gaps = 1/215 (0%) Frame = +1 Query: 184 LALVSSSTGRWXSSPTTRATGPLRSYVAFT-DTERLIGDAAKNXVAMNPNNTIFDAKRLI 360 LA++ T + ++ T P S VAFT D ERL+G +AK +NP NT F KRLI Sbjct: 64 LAIMEGQTPKVIANAEGTRTTP--SVVAFTKDGERLVGVSAKRQAVINPENTFFATKRLI 121 Query: 361 GRKFEDATVQADMKXWPFEVVSDGGXPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAY 540 GR+F++ VQ D+K P+++V + KT+ P ++ +L+KM+ETA Y Sbjct: 122 GRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIGGFILSKMRETASTY 178 Query: 541 LGKTVQNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDQKGTGXRH 720 LGK V+NAV+TVPAYFNDSQRQATK AG I+GLNVLR++NEPTAAA+AYGLD+K Sbjct: 179 LGKDVKNAVVTVPAYFNDSQRQATKAAGAIAGLNVLRVVNEPTAAALAYGLDKKNDAI-- 236 Query: 721 VLIFDLGGGTFDVSILTIEDGIFXVKSTAGDTHLG 825 V +FDLGGGTFD+SIL + +G+F V+ST GDTHLG Sbjct: 237 VAVFDLGGGTFDISILELNNGVFEVRSTNGDTHLG 271 Score = 43.2 bits (97), Expect = 5e-05 Identities = 18/37 (48%), Positives = 24/37 (64%) Frame = +3 Query: 144 KAPAVGIDLGTXYSCVGVFQHGKVEXIANDQGNRTTP 254 K P +GIDLGT SC+ + + + IAN +G RTTP Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTP 85 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 118 bits (285), Expect = 8e-28 Identities = 69/195 (35%), Positives = 107/195 (54%), Gaps = 5/195 (2%) Frame = +1 Query: 256 SYVAFTDTERLIGDAAKNXVAMNPNNTIFDAKRLIGRKFEDATVQ-ADMKXWPFEVVSDG 432 S V++ + R +G+AAK+ A N NT+ KRL GR ++D ++ + ++ Sbjct: 41 SLVSYGERSRFLGEAAKSAEASNFRNTVGSLKRLAGRTYDDPEIKDIESNFISAKLTEVD 100 Query: 433 GXPKIKVAYKGEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQAT 612 G KV Y E+ F ++ + TK+K AEA L +V + VI++PA+F D QR+A Sbjct: 101 GFVGAKVQYLNEETAFSNIQLIAAYFTKIKAIAEAELIGSVSDVVISIPAWFTDIQRRAL 160 Query: 613 KDAGTISGLNVLRIINEPTAAAIAYGLDQ----KGTGXRHVLIFDLGGGTFDVSILTIED 780 +A I+GLN LR++N+ AAA+ YG+ + + R V I D G + VSI+ Sbjct: 161 LEAANIAGLNPLRLMNDNAAAALTYGITKTDLPEPESPRRVAIVDFGHSNYSVSIVEFSR 220 Query: 781 GIFXVKSTAGDTHLG 825 G F +KST D +LG Sbjct: 221 GQFHIKSTVCDRNLG 235 Score = 31.9 bits (69), Expect = 0.12 Identities = 13/40 (32%), Positives = 23/40 (57%) Frame = +3 Query: 141 AKAPAVGIDLGTXYSCVGVFQHGKVEXIANDQGNRTTPXL 260 ++ VGID G + + V ++ ++ I N+ NR+TP L Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSL 42 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 81.8 bits (193), Expect = 1e-16 Identities = 51/195 (26%), Positives = 100/195 (51%), Gaps = 5/195 (2%) Frame = +1 Query: 256 SYVAFTDTERLIGDAAKNXVAMNPNNTIFDAKRLIGRKFEDATVQ-ADMKXWPFEVVSDG 432 S +++ + G A+ + N +N++ + + L+G+ ++ T+ P V Sbjct: 60 SILSYHGDQEYHGVQARGQLVRNADNSVTNFRDLLGKSHDELTLHHCHYSANPVNVEGQI 119 Query: 433 GXPKIKVAYKGE----DKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQ 600 G KI V E +K E S L ++ E+AE +LG V V++VP YF D+Q Sbjct: 120 GF-KITVQEDEESDPKEKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQ 178 Query: 601 RQATKDAGTISGLNVLRIINEPTAAAIAYGLDQKGTGXRHVLIFDLGGGTFDVSILTIED 780 R+A + A +GL VL++I++P A +A ++ + V++ + G +VS+++++ Sbjct: 179 RKALESAANEAGLPVLQLIHDPAAVILALMYSEEVLIDKTVVVANFGATRSEVSVVSVKG 238 Query: 781 GIFXVKSTAGDTHLG 825 G+ + ++ D +LG Sbjct: 239 GLMTILASVHDENLG 253 Score = 35.1 bits (77), Expect = 0.013 Identities = 14/45 (31%), Positives = 23/45 (51%) Frame = +3 Query: 156 VGIDLGTXYSCVGVFQHGKVEXIANDQGNRTTPXLCCVHRHRASH 290 +GI G S + + GK + +AN++GNR P + H + H Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYH 71 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 69.7 bits (163), Expect = 5e-13 Identities = 45/178 (25%), Positives = 85/178 (47%), Gaps = 3/178 (1%) Frame = +1 Query: 253 RSYVAFTDTERLIGDAAKNXVAMNPNNTIFDAKRLIGRK-FEDATVQADMKXWPFEVVSD 429 +S VAF ER+ G A N P ++I + K L+ E VQ +P + + Sbjct: 58 QSAVAFKGNERIFGVDASNLATRFPAHSIRNVKELLDTAGLESVLVQKYQSSYPAIQLVE 117 Query: 430 GGXPKIKVAYK-GEDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFNDSQRQ 606 +++ +++ + EE+ +M + AE + + + V+TVP +FN+ QR Sbjct: 118 NEETTSGISFVISDEENYSLEEIIAMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQRS 177 Query: 607 ATKDAGTISGLNVLRIINEPTAAAIAYGLDQK-GTGXRHVLIFDLGGGTFDVSILTIE 777 +A I +VL +I++ A AI Y L + T + +I+D G G+ ++++ + Sbjct: 178 ILLEAARILNKHVLALIDDNVAVAIEYSLSRSFSTDPTYNIIYDSGSGSTSATVISFD 235 >SPBC18A7.01 ||SPBC4F6.19c|X-Pro dipeptidase |Schizosaccharomyces pombe|chr 2|||Manual Length = 451 Score = 31.1 bits (67), Expect = 0.21 Identities = 19/76 (25%), Positives = 34/76 (44%) Frame = +1 Query: 262 VAFTDTERLIGDAAKNXVAMNPNNTIFDAKRLIGRKFEDATVQADMKXWPFEVVSDGGXP 441 + F+D E GD A + P + AK L+G+ + + D P++V+ D P Sbjct: 125 IIFSDDEPYPGDVASRIYFLVPKFELPRAKELVGKNIDAKYITWDEDENPYQVLYDRLGP 184 Query: 442 KIKVAYKGEDKTFFPE 489 +K+ G + F + Sbjct: 185 -LKLMIDGTVRNFIAQ 199 >SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosaccharomyces pombe|chr 1|||Manual Length = 378 Score = 28.7 bits (61), Expect = 1.1 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +1 Query: 148 HPQ*XSIWVPRTLALVSSSTGRWXSSPTTRAT 243 HP S W+P LVS+ W +SP+++ T Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314 >SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1236 Score = 26.2 bits (55), Expect = 6.0 Identities = 30/140 (21%), Positives = 57/140 (40%), Gaps = 1/140 (0%) Frame = -2 Query: 785 IPSSMVRMDTSKVPPPRSKISTCRSPVPFWSRP*AIAAAVGS-LMIRRTFKPEMVPASFV 609 IP+S +S + S IS+ P F S ++++ S + ++F Sbjct: 545 IPTSSSSDFSSSITTISSGISSSSIPSTFSSVSSILSSSTSSPSSTSLSISSSSTSSTFS 604 Query: 608 ACL*ESLKYAGTVITAFCTVLPR*ASAVSFIFVSTMELTSSGKKVLSSPLXATLILGXPP 429 + S + I++ T+L + S + +S+ + S +LSS + I Sbjct: 605 SASTSSPSSISSSISSSSTILSSPTPSTSSLMISSSSIISGSSSILSSSISTIPI---SS 661 Query: 428 SLTTSKGQXFMSACTVASSN 369 SL+T S+ T+ SS+ Sbjct: 662 SLSTYSSSVIPSSSTLVSSS 681 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,272,935 Number of Sequences: 5004 Number of extensions: 62821 Number of successful extensions: 204 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 162 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 196 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 432473040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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