BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP03_F_P21 (910 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC685.07c |rpl2701|rpl27-1|60S ribosomal protein L27|Schizosac... 157 2e-39 SPCC74.05 |rpl2702|rpl27-2|60S ribosomal protein L27|Schizosacch... 155 1e-38 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 33 0.074 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 33 0.074 SPCC622.18 |rpl6||60S ribosomal protein L6|Schizosaccharomyces p... 31 0.17 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 31 0.17 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 0.91 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 29 1.2 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 29 1.2 SPAC144.08 |||DNAJ domain protein Jac1 |Schizosaccharomyces pomb... 28 2.1 SPAC1805.13 |rpl14||60S ribosomal protein L14|Schizosaccharomyce... 27 2.8 SPBC27.03 |meu25||sequence orphan|Schizosaccharomyces pombe|chr ... 27 2.8 SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 24 4.5 SPAC25G10.04c |rec10|rec20|meiotic recombination protein Rec10 |... 27 4.9 SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 27 4.9 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 26 8.5 SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr... 26 8.5 >SPBC685.07c |rpl2701|rpl27-1|60S ribosomal protein L27|Schizosaccharomyces pombe|chr 2|||Manual Length = 136 Score = 157 bits (381), Expect = 2e-39 Identities = 69/136 (50%), Positives = 96/136 (70%), Gaps = 2/136 (1%) Frame = +1 Query: 82 MGKIMKPGKVVLVLSGRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKN 261 M KI+KPGKV L+ GR+AG+K ++++ D+G+ P+GHA VAG++RYP KV K MG Sbjct: 1 MVKILKPGKVALITRGRFAGKKVVILQAIDQGSKSHPFGHAVVAGVERYPLKVTKSMGAK 60 Query: 262 KIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEK--FSAKDLKDPAKRKKLRFNTRVRFEE 435 +I +RS++KPF+KVVNYNHLMPTRY ++ K +A K+P +R R + FEE Sbjct: 61 RIARRSRVKPFIKVVNYNHLMPTRYALELDNLKGLITADTFKEPTQRSAARKTVKKTFEE 120 Query: 436 RYKSGKNKWFFQKLRF 483 +Y+SGK+ WFF LRF Sbjct: 121 KYQSGKSAWFFTPLRF 136 >SPCC74.05 |rpl2702|rpl27-2|60S ribosomal protein L27|Schizosaccharomyces pombe|chr 3|||Manual Length = 136 Score = 155 bits (375), Expect = 1e-38 Identities = 67/136 (49%), Positives = 96/136 (70%), Gaps = 2/136 (1%) Frame = +1 Query: 82 MGKIMKPGKVVLVLSGRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKN 261 M KI+KPGKV LV GR+AG+K ++++N D+G+ P+GHA VAG++RYP KV K MG Sbjct: 1 MVKILKPGKVALVTRGRFAGKKVVILQNVDQGSKSHPFGHAVVAGVERYPLKVTKSMGAK 60 Query: 262 KIHKRSKIKPFVKVVNYNHLMPTRYTVDFSFEK--FSAKDLKDPAKRKKLRFNTRVRFEE 435 +I KRS++KPF+KV+NYNHLMPTRY ++ K + +P++R + + FEE Sbjct: 61 RIAKRSRVKPFIKVINYNHLMPTRYALELDNLKGLVTPTTFSEPSQRSAAKKTVKNTFEE 120 Query: 436 RYKSGKNKWFFQKLRF 483 +Y++GK+ WFF LRF Sbjct: 121 KYQTGKSAWFFTPLRF 136 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 32.7 bits (71), Expect = 0.074 Identities = 20/58 (34%), Positives = 24/58 (41%), Gaps = 5/58 (8%) Frame = +3 Query: 693 PPPPHXKXXGPXTXXXFGQXXXPPPPPXXAPP----XPPXKNXXPPPXXP-FPXXFPP 851 PP P + P + + PPP P APP PP + PPP P P PP Sbjct: 130 PPTPQSELRPPTSAPP--RPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPP 185 Score = 26.2 bits (55), Expect = 6.4 Identities = 16/54 (29%), Positives = 17/54 (31%), Gaps = 1/54 (1%) Frame = +3 Query: 693 PPPPHXKXXGPXTXXXFGQXXXPPPPPXXAPPXPPXKNXXPPPXXP-FPXXFPP 851 PP P P + PP P PP P PP P P PP Sbjct: 144 PPRPSIPPPSPASAPPI-PSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPP 196 Score = 26.2 bits (55), Expect = 6.4 Identities = 14/53 (26%), Positives = 14/53 (26%) Frame = +3 Query: 693 PPPPHXKXXGPXTXXXFGQXXXPPPPPXXAPPXPPXKNXXPPPXXPFPXXFPP 851 PP P P PP P PP P P P PP Sbjct: 151 PPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPP 203 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 32.7 bits (71), Expect = 0.074 Identities = 16/49 (32%), Positives = 18/49 (36%) Frame = +2 Query: 761 PPXXFXGXPXSPXQKPXSPXXXPLPFXFPPXPRXRXXXXXFPPTKXPPP 907 PP G P +P P +P PLP P P PP P P Sbjct: 436 PPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPAP 484 Score = 32.3 bits (70), Expect = 0.098 Identities = 13/31 (41%), Positives = 14/31 (45%) Frame = +3 Query: 693 PPPPHXKXXGPXTXXXFGQXXXPPPPPXXAP 785 PPPP G G+ PPPPP AP Sbjct: 340 PPPPRSNAAGSIPLPPQGRSAPPPPPPRSAP 370 Score = 31.9 bits (69), Expect = 0.13 Identities = 18/53 (33%), Positives = 20/53 (37%), Gaps = 7/53 (13%) Frame = +3 Query: 693 PPPPHXKXXG-PXTXXXFGQXXXPPPPP------XXAPPXPPXKNXXPPPXXP 830 PPPP + G P PPPPP + P PP PPP P Sbjct: 314 PPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPP 366 Score = 27.1 bits (57), Expect = 3.7 Identities = 18/74 (24%), Positives = 19/74 (25%), Gaps = 1/74 (1%) Frame = +3 Query: 612 PGXGXSPXXXGXXGPPKXXKXXLGGXXPPPPHXKXXGPXTXXXFGQXXXPPPPPXXAPP- 788 P S G PP + PPP G PP PP Sbjct: 363 PPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPS 422 Query: 789 XPPXKNXXPPPXXP 830 PP PP P Sbjct: 423 LPPSAPPSLPPSAP 436 Score = 25.8 bits (54), Expect = 8.5 Identities = 12/31 (38%), Positives = 12/31 (38%) Frame = +3 Query: 759 PPPPPXXAPPXPPXKNXXPPPXXPFPXXFPP 851 PP P APP P PP P PP Sbjct: 428 PPSLPPSAPPSLPMGAPAAPPLPPSAPIAPP 458 >SPCC622.18 |rpl6||60S ribosomal protein L6|Schizosaccharomyces pombe|chr 3|||Manual Length = 195 Score = 31.5 bits (68), Expect = 0.17 Identities = 10/34 (29%), Positives = 23/34 (67%) Frame = +1 Query: 73 PSKMGKIMKPGKVVLVLSGRYAGRKAIVVKNYDE 174 P+K+ + PG V ++L+GR+ G++ +V+ ++ Sbjct: 45 PTKLRASLAPGTVCILLAGRFRGKRVVVLSQLED 78 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 31.5 bits (68), Expect = 0.17 Identities = 22/73 (30%), Positives = 22/73 (30%) Frame = +3 Query: 612 PGXGXSPXXXGXXGPPKXXKXXLGGXXPPPPHXKXXGPXTXXXFGQXXXPPPPPXXAPPX 791 P P G P K G PP P P G P PPP APP Sbjct: 1164 PSVAAPPVPAPSSGIPPVPKPAAG--VPPVPPPSEAPPVPKPSVG--VPPVPPPSTAPPV 1219 Query: 792 PPXKNXXPPPXXP 830 P PP P Sbjct: 1220 PTPSAGLPPVPVP 1232 Score = 28.3 bits (60), Expect = 1.6 Identities = 15/53 (28%), Positives = 16/53 (30%) Frame = +3 Query: 693 PPPPHXKXXGPXTXXXFGQXXXPPPPPXXAPPXPPXKNXXPPPXXPFPXXFPP 851 PP P P G PPP P + P P P P PP Sbjct: 1041 PPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIPP 1093 Score = 27.5 bits (58), Expect = 2.8 Identities = 17/53 (32%), Positives = 17/53 (32%) Frame = +3 Query: 693 PPPPHXKXXGPXTXXXFGQXXXPPPPPXXAPPXPPXKNXXPPPXXPFPXXFPP 851 PP P P P P P APP P PP P P PP Sbjct: 1092 PPVPKPSVAAPPVPKP-SVAVPPVPAPSGAPPVPKPSVAAPP--VPVPSGAPP 1141 Score = 27.1 bits (57), Expect = 3.7 Identities = 15/50 (30%), Positives = 16/50 (32%) Frame = +2 Query: 758 PPPXXFXGXPXSPXQKPXSPXXXPLPFXFPPXPRXRXXXXXFPPTKXPPP 907 P P G P P P P P PP P+ PP PP Sbjct: 1170 PVPAPSSGIPPVPKPAAGVPPVPP-PSEAPPVPKPSVGVPPVPPPSTAPP 1218 Score = 26.2 bits (55), Expect = 6.4 Identities = 14/46 (30%), Positives = 15/46 (32%) Frame = +3 Query: 693 PPPPHXKXXGPXTXXXFGQXXXPPPPPXXAPPXPPXKNXXPPPXXP 830 PP P P G P P APP P + PP P Sbjct: 1140 PPVPKPSVAAPPVPAPSGAPPV-PKPSVAAPPVPAPSSGIPPVPKP 1184 Score = 26.2 bits (55), Expect = 6.4 Identities = 12/43 (27%), Positives = 12/43 (27%) Frame = +3 Query: 693 PPPPHXKXXGPXTXXXFGQXXXPPPPPXXAPPXPPXKNXXPPP 821 PP P P P P PP PP P P Sbjct: 1159 PPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVP 1201 Score = 25.8 bits (54), Expect = 8.5 Identities = 19/73 (26%), Positives = 22/73 (30%) Frame = +3 Query: 612 PGXGXSPXXXGXXGPPKXXKXXLGGXXPPPPHXKXXGPXTXXXFGQXXXPPPPPXXAPPX 791 P +P G P K + PP P P G P P P A P Sbjct: 1077 PAPSGAPPVPAPSGIPPVPKPSVAA--PPVPKPSVAVPPVPAPSGAP--PVPKPSVAAPP 1132 Query: 792 PPXKNXXPPPXXP 830 P + PP P Sbjct: 1133 VPVPSGAPPVPKP 1145 Score = 25.8 bits (54), Expect = 8.5 Identities = 13/31 (41%), Positives = 13/31 (41%) Frame = +3 Query: 759 PPPPPXXAPPXPPXKNXXPPPXXPFPXXFPP 851 P P P APP P PP P P PP Sbjct: 1132 PVPVPSGAPPVPKPSVAAPP--VPAPSGAPP 1160 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 29.1 bits (62), Expect = 0.91 Identities = 11/22 (50%), Positives = 11/22 (50%) Frame = +3 Query: 765 PPPXXAPPXPPXKNXXPPPXXP 830 PPP APP P PPP P Sbjct: 1715 PPPPSAPPMPAGPPSAPPPPLP 1736 Score = 27.9 bits (59), Expect = 2.1 Identities = 12/38 (31%), Positives = 14/38 (36%) Frame = +1 Query: 796 PPKTXFPXXXXPSLXXSPPPXXPXXAXXFPXXXXPPXP 909 PP+ P P + PPP P P PP P Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736 Score = 27.1 bits (57), Expect = 3.7 Identities = 15/55 (27%), Positives = 16/55 (29%) Frame = +1 Query: 700 PPTXXXPXXXPXXFLAXXXPPPXXLXXXPLXPPPKTXFPXXXXPSLXXSPPPXXP 864 PP P PPP + P PP P P L S P P Sbjct: 1690 PPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVP 1744 Score = 27.1 bits (57), Expect = 3.7 Identities = 13/30 (43%), Positives = 14/30 (46%), Gaps = 1/30 (3%) Frame = +3 Query: 765 PPPXXAP-PXPPXKNXXPPPXXPFPXXFPP 851 PP AP P PP + PPP P PP Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGPP 1728 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 28.7 bits (61), Expect = 1.2 Identities = 13/29 (44%), Positives = 13/29 (44%) Frame = +3 Query: 744 GQXXXPPPPPXXAPPXPPXKNXXPPPXXP 830 G PPPPP PP P PPP P Sbjct: 7 GNPPPPPPPPGFEPPSQP-----PPPPPP 30 Score = 26.2 bits (55), Expect = 6.4 Identities = 10/23 (43%), Positives = 10/23 (43%) Frame = +3 Query: 768 PPXXAPPXPPXKNXXPPPXXPFP 836 PP PP PP PP P P Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPP 27 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 28.7 bits (61), Expect = 1.2 Identities = 14/42 (33%), Positives = 14/42 (33%) Frame = +3 Query: 693 PPPPHXKXXGPXTXXXFGQXXXPPPPPXXAPPXPPXKNXXPP 818 P P P G PPPPP A PP PP Sbjct: 742 PTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783 Score = 27.9 bits (59), Expect = 2.1 Identities = 15/51 (29%), Positives = 15/51 (29%) Frame = +1 Query: 757 PPPXXLXXXPLXPPPKTXFPXXXXPSLXXSPPPXXPXXAXXFPXXXXPPXP 909 PPP P P P PPP P A P PP P Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783 Score = 27.1 bits (57), Expect = 3.7 Identities = 18/52 (34%), Positives = 18/52 (34%), Gaps = 4/52 (7%) Frame = +3 Query: 693 PPPPHXKXXGPXTXXXFGQXXXPPPPPXXAPPXPP----XKNXXPPPXXPFP 836 PPPP P T PP P PP PP PPP P P Sbjct: 732 PPPPPPAVIVP-TPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782 >SPAC144.08 |||DNAJ domain protein Jac1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 225 Score = 27.9 bits (59), Expect = 2.1 Identities = 16/46 (34%), Positives = 22/46 (47%), Gaps = 1/46 (2%) Frame = +1 Query: 253 GKNKIHKRSKIKPFVKVVNYNHLMPTRYTVDF-SFEKFSAKDLKDP 387 G + I R K P K+VNYNH + + +F K D+ DP Sbjct: 28 GNHWIFARFKFYPLNKIVNYNHFHSSSCQSEAKNFYKQFEGDISDP 73 >SPAC1805.13 |rpl14||60S ribosomal protein L14|Schizosaccharomyces pombe|chr 1|||Manual Length = 134 Score = 27.5 bits (58), Expect = 2.8 Identities = 11/28 (39%), Positives = 18/28 (64%) Frame = +1 Query: 88 KIMKPGKVVLVLSGRYAGRKAIVVKNYD 171 + ++ G+VVLV G Y G+ A++V D Sbjct: 6 RYVEVGRVVLVTKGEYTGKLAVIVDIVD 33 >SPBC27.03 |meu25||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 622 Score = 27.5 bits (58), Expect = 2.8 Identities = 24/101 (23%), Positives = 45/101 (44%), Gaps = 2/101 (1%) Frame = -2 Query: 336 ITCWHQVIIVYNL-HERLYLGPLVDFILSHPLVHFPG-VPVDTSDESMPVRLVGGTFVVI 163 + W + +I + H +L + P+ F H + FP V V TS + + Sbjct: 485 LALWKKQVIFSKIGHPKLLVMPISSF---HCVQRFPDLVCVQTSTAETFLSVDRELTTYD 541 Query: 162 LDYDSLATRVPAT*DQHYFTRLHYLTHLGGIFPLLVDIESK 40 L+ L P+ Q++ +H+L +G +FP LV ++ + Sbjct: 542 LNTIKLLNTAPSRTAQNWLFIMHHLEIVGYLFPHLVHLDKQ 582 >SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 1420 Score = 24.2 bits (50), Expect(2) = 4.5 Identities = 12/38 (31%), Positives = 13/38 (34%) Frame = +1 Query: 760 PPXXLXXXPLXPPPKTXFPXXXXPSLXXSPPPXXPXXA 873 P + P PP P PS PPP P A Sbjct: 161 PNSMVSPPPSFQPPSAAAPATSLPSDYNPPPPPPPPPA 198 Score = 20.6 bits (41), Expect(2) = 4.5 Identities = 8/21 (38%), Positives = 8/21 (38%) Frame = +1 Query: 847 PPPXXPXXAXXFPXXXXPPXP 909 PP P A P PP P Sbjct: 224 PPTYTPKQADPLPAPPPPPPP 244 >SPAC25G10.04c |rec10|rec20|meiotic recombination protein Rec10 |Schizosaccharomyces pombe|chr 1|||Manual Length = 791 Score = 26.6 bits (56), Expect = 4.9 Identities = 13/43 (30%), Positives = 19/43 (44%) Frame = -3 Query: 188 LSEVPSS*FLTTIALRPAYRPLKTSTTLPGFIILPILEGYSLY 60 L E+P + F T+ AY P S T+ G + G +Y Sbjct: 689 LEEIPYNKFFKTVEKNEAYNPSSKSATIDGLQRYTSMIGNQIY 731 >SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr 2|||Manual Length = 305 Score = 26.6 bits (56), Expect = 4.9 Identities = 18/50 (36%), Positives = 19/50 (38%) Frame = -2 Query: 909 GGGGXXVGGKXXXXXRXRGXGGXXKGRGXXXGEXGFWXGXKGXPXKXXGG 760 GG G GG+ R G GG GRG G G G G GG Sbjct: 23 GGRGGFGGGRGGA--RGGGRGGARGGRGGRGGARGGRGGSSGGRGGAKGG 70 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 25.8 bits (54), Expect = 8.5 Identities = 15/38 (39%), Positives = 19/38 (50%) Frame = -2 Query: 282 LGPLVDFILSHPLVHFPGVPVDTSDESMPVRLVGGTFV 169 LGP+ + + L HFP DT + S P GGT V Sbjct: 1364 LGPIGETFEVNGLSHFPSDIEDTIERSHPRIARGGTAV 1401 >SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr 1|||Manual Length = 872 Score = 25.8 bits (54), Expect = 8.5 Identities = 14/34 (41%), Positives = 19/34 (55%) Frame = -2 Query: 384 VFQVFCAEFFKAEVNCITCWHQVIIVYNLHERLY 283 V+QVFC F A+ I +Q++ V NL E Y Sbjct: 315 VYQVFCTNFPNAQQLIIDNQNQLLKVANLLEPSY 348 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,024,825 Number of Sequences: 5004 Number of extensions: 60241 Number of successful extensions: 353 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 178 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 274 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 460503700 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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