BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP03_F_P13 (858 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g22060.1 68414.m02759 expressed protein 31 0.98 At1g52560.1 68414.m05933 26.5 kDa class I small heat shock prote... 29 3.0 At3g18370.1 68416.m02336 C2 domain-containing protein contains P... 29 5.2 At5g57230.1 68418.m07150 expressed protein 28 9.1 At5g55540.1 68418.m06919 expressed protein 28 9.1 At5g12970.1 68418.m01487 C2 domain-containing protein contains I... 28 9.1 At5g04970.1 68418.m00526 pectinesterase, putative contains simil... 28 9.1 >At1g22060.1 68414.m02759 expressed protein Length = 1999 Score = 31.1 bits (67), Expect = 0.98 Identities = 13/37 (35%), Positives = 22/37 (59%) Frame = +2 Query: 200 ESMLDTHSLWSNLANEMQHLDNMMKELSLKFPSIINE 310 E MLDT +S++ E++ + + +LSLKF + E Sbjct: 1932 EEMLDTKGRYSSMETELREMHDRYSQLSLKFAEVEGE 1968 >At1g52560.1 68414.m05933 26.5 kDa class I small heat shock protein-like (HSP26.5-P) contains Pfam profile: PF00011 Hsp20/alpha crystallin family; identified in Scharf, K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237. Length = 232 Score = 29.5 bits (63), Expect = 3.0 Identities = 12/79 (15%), Positives = 40/79 (50%) Frame = +2 Query: 182 FSPYVRESMLDTHSLWSNLANEMQHLDNMMKELSLKFPSIINEGRVEGDKYQISIHLPGY 361 F+P + E T + + L ++++ + ++ ++ + + + D Y++ +PG Sbjct: 88 FTPTLNEFFPPT--IGNTLIQATENMNRIFDNFNVNPFQLMGQVKEQDDCYKLRYEVPGL 145 Query: 362 EQKDINVKAKNGVLMVQAN 418 ++D+ + +G+L ++ + Sbjct: 146 TKEDVKITVNDGILTIKGD 164 >At3g18370.1 68416.m02336 C2 domain-containing protein contains Pfam profile: PF00168 C2 domain Length = 815 Score = 28.7 bits (61), Expect = 5.2 Identities = 11/28 (39%), Positives = 20/28 (71%) Frame = +2 Query: 242 NEMQHLDNMMKELSLKFPSIINEGRVEG 325 N+ L N MK++ +KF ++I++G +EG Sbjct: 735 NKALLLSNQMKQVMIKFQNLIDDGDLEG 762 >At5g57230.1 68418.m07150 expressed protein Length = 160 Score = 27.9 bits (59), Expect = 9.1 Identities = 14/32 (43%), Positives = 19/32 (59%) Frame = +2 Query: 239 ANEMQHLDNMMKELSLKFPSIINEGRVEGDKY 334 +N QHLD M++E + +F I RVE KY Sbjct: 60 SNYTQHLDRMLEEAAAEFYPNIKFMRVECPKY 91 >At5g55540.1 68418.m06919 expressed protein Length = 1380 Score = 27.9 bits (59), Expect = 9.1 Identities = 13/30 (43%), Positives = 18/30 (60%) Frame = +2 Query: 260 DNMMKELSLKFPSIINEGRVEGDKYQISIH 349 D + SL PSI+ EGR + K+QI+ H Sbjct: 866 DPSSPDSSLLVPSILEEGRGKTQKWQINTH 895 >At5g12970.1 68418.m01487 C2 domain-containing protein contains INTERPRO:IPR000008 C2 domain Length = 769 Score = 27.9 bits (59), Expect = 9.1 Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%) Frame = +1 Query: 292 PQHYKRRTR-GRRQVSDIYSPAWLRTERHQRESEKWSADGA 411 PQ Y+ R GR+ ++ W+ T+ + S+ W +D A Sbjct: 140 PQWYRLEDRHGRKVKGELMLAVWMGTQADEAFSDAWHSDAA 180 >At5g04970.1 68418.m00526 pectinesterase, putative contains similarity to pectinesterase from Vitis vinifera GI:15081598, Prunus persica SP|Q43062; contains Pfam profile PF01095 pectinesterase Length = 624 Score = 27.9 bits (59), Expect = 9.1 Identities = 25/90 (27%), Positives = 37/90 (41%), Gaps = 2/90 (2%) Frame = +1 Query: 508 NHLPAEXKAAXG*QEASCPSPLTPPLXM*VVKRWSSPPRATCGTLTSXLETSPEHPMRSP 687 +H P + Q S PSPL P + S+P C T+ S +++SP P Sbjct: 61 SHPPTQPPTPPPSQSPSQPSPLPPNIAC-----KSTPYPKLCRTILSAVKSSPSDPYHYG 115 Query: 688 KAVQXTTLXXPHQRXCGVLARF--HIKFDP 771 K L +R V+ RF ++ DP Sbjct: 116 KFTMKQCLKQA-RRLSKVINRFAQRVEADP 144 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,185,861 Number of Sequences: 28952 Number of extensions: 343437 Number of successful extensions: 978 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 939 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 975 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1999652000 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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