Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP03_F_P12
         (841 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha...    63   5e-11
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    59   9e-10
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    43   5e-05
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    41   3e-04
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    36   0.007
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch...    27   3.3  
SPAC4F8.14c |hcs1|hcs|3-hydroxy-3-methylglutaryl-CoA synthase|Sc...    27   4.4  

>SPAC926.03 |rlc1||myosin II regulatory light chain
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 184

 Score = 62.9 bits (146), Expect = 5e-11
 Identities = 30/83 (36%), Positives = 45/83 (54%)
 Frame = +2

Query: 215 SRKAKRTGSNVFSMFSQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELD 394
           ++ AKR  S  F+  +  Q+ E KEAF L+D D DG IG+ D++    SL + ASE  ++
Sbjct: 28  AQAAKRASSGAFAQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSIN 87

Query: 395 EMVGEASGPINFXQLLTLFANRM 463
            M    + PIN    LT   + +
Sbjct: 88  HMFESINPPINLAAFLTAMGSML 110


>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 58.8 bits (136), Expect = 9e-10
 Identities = 29/72 (40%), Positives = 44/72 (61%), Gaps = 4/72 (5%)
 Frame = +2

Query: 260 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGE----ASGPIN 427
           + +Q+AEF+EAF L D D+DG I  N+L     SLG+  +  EL +M+ E     +G I+
Sbjct: 7   TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66

Query: 428 FXQLLTLFANRM 463
           F + LT+ A +M
Sbjct: 67  FTEFLTMMARKM 78



 Score = 37.1 bits (82), Expect = 0.003
 Identities = 20/65 (30%), Positives = 36/65 (55%), Gaps = 4/65 (6%)
 Frame = +2

Query: 278 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEA----SGPINFXQLLT 445
           E +EAF++ D D +G I   +L     SLG   S++E+ +M+ EA     G IN+ +   
Sbjct: 86  EVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSR 145

Query: 446 LFANR 460
           + +++
Sbjct: 146 VISSK 150



 Score = 27.5 bits (58), Expect = 2.5
 Identities = 14/35 (40%), Positives = 20/35 (57%), Gaps = 2/35 (5%)
 Frame = +3

Query: 474 SDEDDVVINAFKTFDEEGK--IXSXRLRHALMTLG 572
           +D ++ V  AFK FD++G   I    L H L +LG
Sbjct: 81  TDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLG 115


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 43.2 bits (97), Expect = 5e-05
 Identities = 22/68 (32%), Positives = 39/68 (57%)
 Frame = +2

Query: 260 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMVGEASGPINFXQL 439
           S++Q  E KEAF L D DKDG+I  + + +   SLG   ++ EL ++  E    I+  + 
Sbjct: 4   SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63

Query: 440 LTLFANRM 463
           ++  +N++
Sbjct: 64  MSFVSNKL 71


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 40.7 bits (91), Expect = 3e-04
 Identities = 18/42 (42%), Positives = 26/42 (61%)
 Frame = +2

Query: 278 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 403
           EF + FQ+ D D  G+IG  +LR    SLG   S +E+DE++
Sbjct: 78  EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELL 119


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 35.9 bits (79), Expect = 0.007
 Identities = 17/44 (38%), Positives = 27/44 (61%)
 Frame = +2

Query: 260 SQKQVAEFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKEL 391
           +++Q  +  EAF+L D DKD  I  ++LRA   +LG  A + E+
Sbjct: 32  TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEV 75


>SPAC1687.14c |||EF hand family protein, unknown
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 76

 Score = 27.1 bits (57), Expect = 3.3
 Identities = 15/42 (35%), Positives = 22/42 (52%)
 Frame = +2

Query: 278 EFKEAFQLMDHDKDGIIGKNDLRATFDSLGRLASEKELDEMV 403
           E +EAF L D    G I   DLR +   LG   ++++L  M+
Sbjct: 13  EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLML 54


>SPAC4F8.14c |hcs1|hcs|3-hydroxy-3-methylglutaryl-CoA
           synthase|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 447

 Score = 26.6 bits (56), Expect = 4.4
 Identities = 16/49 (32%), Positives = 27/49 (55%), Gaps = 3/49 (6%)
 Frame = -1

Query: 526 PSSSKVLKA---LITTSSSSEPAGHAVGEQSQELXEVDGARSLTDHLIE 389
           P+  +V KA   L+ T S++EP+   +    + L  +D  +SLTD  +E
Sbjct: 252 PTCKQVQKAYARLLYTDSAAEPSNPELEGVRELLSTLDAKKSLTDKALE 300


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,125,458
Number of Sequences: 5004
Number of extensions: 28821
Number of successful extensions: 107
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 102
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 107
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 414453330
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)