BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP03_F_P04 (850 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g59760.3 68416.m06669 cysteine synthase, mitochondrial, putat... 65 5e-11 At3g59760.2 68416.m06668 cysteine synthase, mitochondrial, putat... 65 5e-11 At3g59760.1 68416.m06667 cysteine synthase, mitochondrial, putat... 65 5e-11 At3g22460.1 68416.m02839 cysteine synthase, putative / O-acetyls... 64 1e-10 At4g14880.2 68417.m02286 cysteine synthase / O-acetylserine (thi... 62 3e-10 At4g14880.1 68417.m02285 cysteine synthase / O-acetylserine (thi... 62 3e-10 At5g28020.2 68418.m03375 cysteine synthase, putative / O-acetyls... 62 5e-10 At5g28020.1 68418.m03374 cysteine synthase, putative / O-acetyls... 62 5e-10 At2g43750.1 68415.m05439 cysteine synthase, chloroplast / O-acet... 62 6e-10 At5g28030.2 68418.m03377 cysteine synthase, putative / O-acetyls... 58 7e-09 At5g28030.1 68418.m03376 cysteine synthase, putative / O-acetyls... 58 7e-09 At3g04940.1 68416.m00536 cysteine synthase, putative / O-acetyls... 58 7e-09 At3g03630.1 68416.m00366 cysteine synthase, chloroplast, putativ... 48 8e-06 At3g61440.1 68416.m06881 cysteine synthase, putative / O-acetyls... 48 1e-05 At1g55880.2 68414.m06409 pyridoxal-5'-phosphate-dependent enzyme... 45 7e-05 At1g55880.1 68414.m06408 pyridoxal-5'-phosphate-dependent enzyme... 45 7e-05 At5g35750.1 68418.m04281 histidine kinase (AHK2) identical to hi... 29 3.0 At3g60400.1 68416.m06755 mitochondrial transcription termination... 29 3.9 At3g14980.1 68416.m01894 PHD finger transcription factor, putati... 28 9.0 >At3g59760.3 68416.m06669 cysteine synthase, mitochondrial, putative / O-acetylserine (thiol)-lyase, putative / O-acetylserine sulfhydrylase, putative similar to SP|Q43725 Cysteine synthase, mitochondrial precursor (EC 4.2.99.8) (O- acetylserine sulfhydrylase) (O-acetylserine (Thiol)-lyase) {Arabidopsis thaliana} Length = 430 Score = 65.3 bits (152), Expect = 5e-11 Identities = 30/40 (75%), Positives = 32/40 (80%) Frame = +3 Query: 630 SVXDRIAYRMFLDAXQKGILKPGKSVIVEPTSGNTGIGLA 749 SV DRI Y M DA QKG + PGKSV+VEPTSGNTGIGLA Sbjct: 152 SVKDRIGYSMVTDAEQKGFISPGKSVLVEPTSGNTGIGLA 191 >At3g59760.2 68416.m06668 cysteine synthase, mitochondrial, putative / O-acetylserine (thiol)-lyase, putative / O-acetylserine sulfhydrylase, putative similar to SP|Q43725 Cysteine synthase, mitochondrial precursor (EC 4.2.99.8) (O- acetylserine sulfhydrylase) (O-acetylserine (Thiol)-lyase) {Arabidopsis thaliana} Length = 432 Score = 65.3 bits (152), Expect = 5e-11 Identities = 30/40 (75%), Positives = 32/40 (80%) Frame = +3 Query: 630 SVXDRIAYRMFLDAXQKGILKPGKSVIVEPTSGNTGIGLA 749 SV DRI Y M DA QKG + PGKSV+VEPTSGNTGIGLA Sbjct: 152 SVKDRIGYSMVTDAEQKGFISPGKSVLVEPTSGNTGIGLA 191 >At3g59760.1 68416.m06667 cysteine synthase, mitochondrial, putative / O-acetylserine (thiol)-lyase, putative / O-acetylserine sulfhydrylase, putative similar to SP|Q43725 Cysteine synthase, mitochondrial precursor (EC 4.2.99.8) (O- acetylserine sulfhydrylase) (O-acetylserine (Thiol)-lyase) {Arabidopsis thaliana} Length = 433 Score = 65.3 bits (152), Expect = 5e-11 Identities = 30/40 (75%), Positives = 32/40 (80%) Frame = +3 Query: 630 SVXDRIAYRMFLDAXQKGILKPGKSVIVEPTSGNTGIGLA 749 SV DRI Y M DA QKG + PGKSV+VEPTSGNTGIGLA Sbjct: 152 SVKDRIGYSMVTDAEQKGFISPGKSVLVEPTSGNTGIGLA 191 >At3g22460.1 68416.m02839 cysteine synthase, putative / O-acetylserine (thiol)-lyase, putative / O-acetylserine sulfhydrylase, putative nearly identical over 185 amino acids to SP|P47998 Cysteine synthase (EC 4.2.99.8) (O-acetylserine sulfhydrylase) (O-acetylserine (Thiol)-lyase) {Arabidopsis thaliana} Length = 188 Score = 63.7 bits (148), Expect = 1e-10 Identities = 27/40 (67%), Positives = 33/40 (82%) Frame = +3 Query: 630 SVXDRIAYRMFLDAXQKGILKPGKSVIVEPTSGNTGIGLA 749 SV DRI Y M DA KG++KPG+SV++EPTSGNTG+GLA Sbjct: 47 SVKDRIGYSMIADAEAKGLIKPGESVLIEPTSGNTGVGLA 86 Score = 33.1 bits (72), Expect = 0.24 Identities = 17/40 (42%), Positives = 24/40 (60%) Frame = +2 Query: 503 KVHSDILNVIGNTPLVKLSKLPKDEGLKCEMYAKCEFLNP 622 K+ D+ +IGNTPLV L+K+ KD + AK E + P Sbjct: 7 KIAKDVTELIGNTPLVYLNKVAKD--CVGHVAAKLEMMEP 44 >At4g14880.2 68417.m02286 cysteine synthase / O-acetylserine (thiol)-lyase / O-acetylserine sulfhydrylase (OAS1) nearly identical to SP|P47998 Cysteine synthase (EC 4.2.99.8) (O-acetylserine sulfhydrylase) (O-acetylserine (Thiol)-lyase) {Arabidopsis thaliana}; identical to cDNA O-acetylserine lyase (At.OAS.5-8) GI:6983573 Length = 322 Score = 62.5 bits (145), Expect = 3e-10 Identities = 26/40 (65%), Positives = 34/40 (85%) Frame = +3 Query: 630 SVXDRIAYRMFLDAXQKGILKPGKSVIVEPTSGNTGIGLA 749 SV DRI + M DA +KG++KPG+SV++EPTSGNTG+GLA Sbjct: 44 SVKDRIGFSMISDAEKKGLIKPGESVLIEPTSGNTGVGLA 83 Score = 31.1 bits (67), Expect = 0.97 Identities = 31/119 (26%), Positives = 49/119 (41%), Gaps = 3/119 (2%) Frame = +2 Query: 503 KVHSDILNVIGNTPLVKLSKLPKDEGLKCEMYAKCEFLNPGRIRXRPHSLPHVSGCRXEG 682 ++ D+ +IGNTPLV L+ + EG + AK E + P +S +G Sbjct: 4 RIAKDVTELIGNTPLVYLNNVA--EGCVGRVAAKLEMMEPCSSVKDRIGFSMISDAEKKG 61 Query: 683 YFEAWQVSHSRTHIW*HRNRTG---PWPLLSXGTGAIIXLPEKMSDEKVTPLVALGAEI 850 + + I TG + + G II +P MS E+ L+A G E+ Sbjct: 62 LIKPGE----SVLIEPTSGNTGVGLAFTAAAKGYKLIITMPASMSTERRIILLAFGVEL 116 >At4g14880.1 68417.m02285 cysteine synthase / O-acetylserine (thiol)-lyase / O-acetylserine sulfhydrylase (OAS1) nearly identical to SP|P47998 Cysteine synthase (EC 4.2.99.8) (O-acetylserine sulfhydrylase) (O-acetylserine (Thiol)-lyase) {Arabidopsis thaliana}; identical to cDNA O-acetylserine lyase (At.OAS.5-8) GI:6983573 Length = 322 Score = 62.5 bits (145), Expect = 3e-10 Identities = 26/40 (65%), Positives = 34/40 (85%) Frame = +3 Query: 630 SVXDRIAYRMFLDAXQKGILKPGKSVIVEPTSGNTGIGLA 749 SV DRI + M DA +KG++KPG+SV++EPTSGNTG+GLA Sbjct: 44 SVKDRIGFSMISDAEKKGLIKPGESVLIEPTSGNTGVGLA 83 Score = 31.1 bits (67), Expect = 0.97 Identities = 31/119 (26%), Positives = 49/119 (41%), Gaps = 3/119 (2%) Frame = +2 Query: 503 KVHSDILNVIGNTPLVKLSKLPKDEGLKCEMYAKCEFLNPGRIRXRPHSLPHVSGCRXEG 682 ++ D+ +IGNTPLV L+ + EG + AK E + P +S +G Sbjct: 4 RIAKDVTELIGNTPLVYLNNVA--EGCVGRVAAKLEMMEPCSSVKDRIGFSMISDAEKKG 61 Query: 683 YFEAWQVSHSRTHIW*HRNRTG---PWPLLSXGTGAIIXLPEKMSDEKVTPLVALGAEI 850 + + I TG + + G II +P MS E+ L+A G E+ Sbjct: 62 LIKPGE----SVLIEPTSGNTGVGLAFTAAAKGYKLIITMPASMSTERRIILLAFGVEL 116 >At5g28020.2 68418.m03375 cysteine synthase, putative / O-acetylserine (thiol)-lyase, putative / O-acetylserine sulfhydrylase, putative similar to O-acetylserine(thiol) lyase (EC 4.2.99.8) [Brassica juncea] GI:2245144; contains Pfam profile PF00291: Pyridoxal-phosphate dependent enzyme Length = 323 Score = 62.1 bits (144), Expect = 5e-10 Identities = 27/40 (67%), Positives = 32/40 (80%) Frame = +3 Query: 630 SVXDRIAYRMFLDAXQKGILKPGKSVIVEPTSGNTGIGLA 749 SV DRIAY M DA KG++ PGKS ++EPT+GNTGIGLA Sbjct: 46 SVKDRIAYSMIKDAEDKGLITPGKSTLIEPTAGNTGIGLA 85 Score = 29.1 bits (62), Expect = 3.9 Identities = 14/39 (35%), Positives = 23/39 (58%) Frame = +2 Query: 506 VHSDILNVIGNTPLVKLSKLPKDEGLKCEMYAKCEFLNP 622 + +DI +IGNTP+V L+ + +G + AK E + P Sbjct: 7 IKNDITELIGNTPMVYLNNVV--DGCVARIAAKLEMMEP 43 >At5g28020.1 68418.m03374 cysteine synthase, putative / O-acetylserine (thiol)-lyase, putative / O-acetylserine sulfhydrylase, putative similar to O-acetylserine(thiol) lyase (EC 4.2.99.8) [Brassica juncea] GI:2245144; contains Pfam profile PF00291: Pyridoxal-phosphate dependent enzyme Length = 323 Score = 62.1 bits (144), Expect = 5e-10 Identities = 27/40 (67%), Positives = 32/40 (80%) Frame = +3 Query: 630 SVXDRIAYRMFLDAXQKGILKPGKSVIVEPTSGNTGIGLA 749 SV DRIAY M DA KG++ PGKS ++EPT+GNTGIGLA Sbjct: 46 SVKDRIAYSMIKDAEDKGLITPGKSTLIEPTAGNTGIGLA 85 Score = 29.1 bits (62), Expect = 3.9 Identities = 14/39 (35%), Positives = 23/39 (58%) Frame = +2 Query: 506 VHSDILNVIGNTPLVKLSKLPKDEGLKCEMYAKCEFLNP 622 + +DI +IGNTP+V L+ + +G + AK E + P Sbjct: 7 IKNDITELIGNTPMVYLNNVV--DGCVARIAAKLEMMEP 43 >At2g43750.1 68415.m05439 cysteine synthase, chloroplast / O-acetylserine (thiol)-lyase / O-acetylserine sulfhydrylase / cpACS1 (OASB) identical to SP|P47999 Cysteine synthase, chloroplast precursor (EC 4.2.99.8) (O-acetylserine sulfhydrylase) (O-acetylserine (Thiol)-lyase) (cpACS1) {Arabidopsis thaliana}; identical to cDNA O-acetylserine lyase (At.OAS.7-4) GI:6983575 Length = 392 Score = 61.7 bits (143), Expect = 6e-10 Identities = 28/40 (70%), Positives = 32/40 (80%) Frame = +3 Query: 630 SVXDRIAYRMFLDAXQKGILKPGKSVIVEPTSGNTGIGLA 749 SV DRI Y M DA +KG++ PGKSV+VE TSGNTGIGLA Sbjct: 114 SVKDRIGYSMITDAEEKGLITPGKSVLVESTSGNTGIGLA 153 >At5g28030.2 68418.m03377 cysteine synthase, putative / O-acetylserine (thiol)-lyase, putative / O-acetylserine sulfhydrylase, putative similar to O-acetylserine(thiol) lyase [Brassica juncea] GI:2245144; contains Pfam profile PF00291: Pyridoxal-phosphate dependent enzyme Length = 323 Score = 58.0 bits (134), Expect = 7e-09 Identities = 25/40 (62%), Positives = 30/40 (75%) Frame = +3 Query: 630 SVXDRIAYRMFLDAXQKGILKPGKSVIVEPTSGNTGIGLA 749 S+ DRIAY M DA KG++ PGKS ++E T GNTGIGLA Sbjct: 46 SIKDRIAYSMIKDAEDKGLITPGKSTLIEATGGNTGIGLA 85 Score = 31.1 bits (67), Expect = 0.97 Identities = 14/39 (35%), Positives = 24/39 (61%) Frame = +2 Query: 506 VHSDILNVIGNTPLVKLSKLPKDEGLKCEMYAKCEFLNP 622 + +D+ +IGNTP+V L+K+ +G + AK E + P Sbjct: 7 IKNDVTELIGNTPMVYLNKIV--DGCVARIAAKLEMMEP 43 >At5g28030.1 68418.m03376 cysteine synthase, putative / O-acetylserine (thiol)-lyase, putative / O-acetylserine sulfhydrylase, putative similar to O-acetylserine(thiol) lyase [Brassica juncea] GI:2245144; contains Pfam profile PF00291: Pyridoxal-phosphate dependent enzyme Length = 323 Score = 58.0 bits (134), Expect = 7e-09 Identities = 25/40 (62%), Positives = 30/40 (75%) Frame = +3 Query: 630 SVXDRIAYRMFLDAXQKGILKPGKSVIVEPTSGNTGIGLA 749 S+ DRIAY M DA KG++ PGKS ++E T GNTGIGLA Sbjct: 46 SIKDRIAYSMIKDAEDKGLITPGKSTLIEATGGNTGIGLA 85 Score = 31.1 bits (67), Expect = 0.97 Identities = 14/39 (35%), Positives = 24/39 (61%) Frame = +2 Query: 506 VHSDILNVIGNTPLVKLSKLPKDEGLKCEMYAKCEFLNP 622 + +D+ +IGNTP+V L+K+ +G + AK E + P Sbjct: 7 IKNDVTELIGNTPMVYLNKIV--DGCVARIAAKLEMMEP 43 >At3g04940.1 68416.m00536 cysteine synthase, putative / O-acetylserine (thiol)-lyase, putative / O-acetylserine sulfhydrylase, putative similar to O-acetylserine(thiol) lyase (EC 4.2.99.8) [Brassica juncea] GI:2245144; contains Pfam profile PF00291: Pyridoxal-phosphate dependent enzyme Length = 324 Score = 58.0 bits (134), Expect = 7e-09 Identities = 26/40 (65%), Positives = 31/40 (77%) Frame = +3 Query: 630 SVXDRIAYRMFLDAXQKGILKPGKSVIVEPTSGNTGIGLA 749 SV +RIAY M DA KG++ PGKS ++E TSGNTGIGLA Sbjct: 47 SVKERIAYGMIKDAEDKGLITPGKSTLIEATSGNTGIGLA 86 >At3g03630.1 68416.m00366 cysteine synthase, chloroplast, putative / O-acetylserine (thiol)-lyase, putative / O-acetylserine sulfhydrylase, putative identical to SP|O22682 Probable cysteine synthase, chloroplast precursor {Arabidopsis thaliana}, similar to SP|P31300 Cysteine synthase, chloroplast precursor {Capsicum annuum} Length = 404 Score = 48.0 bits (109), Expect = 8e-06 Identities = 21/40 (52%), Positives = 28/40 (70%) Frame = +3 Query: 630 SVXDRIAYRMFLDAXQKGILKPGKSVIVEPTSGNTGIGLA 749 SV DRI M +A G + P K+V+VEPT+GNTG+G+A Sbjct: 138 SVKDRIGLSMINEAENSGAITPRKTVLVEPTTGNTGLGIA 177 Score = 29.1 bits (62), Expect = 3.9 Identities = 17/58 (29%), Positives = 32/58 (55%) Frame = +2 Query: 455 TLKDMPHIVRALDRNQKVHSDILNVIGNTPLVKLSKLPKDEGLKCEMYAKCEFLNPGR 628 T+ + HI + N + D+ +IG+TP+V L+++ +G ++ AK E + P R Sbjct: 84 TVAEEQHIAESETVN--IAEDVTQLIGSTPMVYLNRV--TDGCLADIAAKLESMEPCR 137 >At3g61440.1 68416.m06881 cysteine synthase, putative / O-acetylserine (thiol)-lyase, putative / O-acetylserine sulfhydrylase, putative identical to cysteine synthase (EC 4.2.99.8) [Arabidopsis thaliana] GI:5824334; contains Pfam profile PF00291: Pyridoxal-phosphate dependent enzyme Length = 368 Score = 47.6 bits (108), Expect = 1e-05 Identities = 21/40 (52%), Positives = 28/40 (70%) Frame = +3 Query: 630 SVXDRIAYRMFLDAXQKGILKPGKSVIVEPTSGNTGIGLA 749 S+ DR A M DA +K ++ PGK+ ++EPTSGN GI LA Sbjct: 88 SIKDRPAIAMIADAEKKKLIIPGKTTLIEPTSGNMGISLA 127 >At1g55880.2 68414.m06409 pyridoxal-5'-phosphate-dependent enzyme, beta family protein similar to SP|P50867 Cysteine synthase (EC 4.2.99.8) (O-acetylserine sulfhydrylase) (O-acetylserine (Thiol)-lyase) [Aspergillus nidulans] {Emericella nidulans}; contains Pfam profile PF00291: Pyridoxal-phosphate dependent enzyme Length = 348 Score = 44.8 bits (101), Expect = 7e-05 Identities = 18/40 (45%), Positives = 29/40 (72%) Frame = +2 Query: 506 VHSDILNVIGNTPLVKLSKLPKDEGLKCEMYAKCEFLNPG 625 + + +++ IGNTPL++++ L + G CE+ KCEFLNPG Sbjct: 40 MRNGLVDAIGNTPLIRINSLSEATG--CEILGKCEFLNPG 77 Score = 39.1 bits (87), Expect = 0.004 Identities = 24/71 (33%), Positives = 37/71 (52%), Gaps = 4/71 (5%) Frame = +3 Query: 612 FSTLGGSVXDRIAYRMFLDAXQKGILKPGKSVIVEPTSGNTGIGLALGR----CCXXVQV 779 F GGSV DR+A ++ +A + G L PG ++ E ++G+T I LA C V + Sbjct: 73 FLNPGGSVKDRVAVKIIQEALESGKLFPG-GIVTEGSAGSTAISLATVAPAYGCKCHVVI 131 Query: 780 PLXCCLRKCQM 812 P + K Q+ Sbjct: 132 PDDAAIEKSQI 142 >At1g55880.1 68414.m06408 pyridoxal-5'-phosphate-dependent enzyme, beta family protein similar to SP|P50867 Cysteine synthase (EC 4.2.99.8) (O-acetylserine sulfhydrylase) (O-acetylserine (Thiol)-lyase) [Aspergillus nidulans] {Emericella nidulans}; contains Pfam profile PF00291: Pyridoxal-phosphate dependent enzyme Length = 421 Score = 44.8 bits (101), Expect = 7e-05 Identities = 18/40 (45%), Positives = 29/40 (72%) Frame = +2 Query: 506 VHSDILNVIGNTPLVKLSKLPKDEGLKCEMYAKCEFLNPG 625 + + +++ IGNTPL++++ L + G CE+ KCEFLNPG Sbjct: 40 MRNGLVDAIGNTPLIRINSLSEATG--CEILGKCEFLNPG 77 Score = 39.1 bits (87), Expect = 0.004 Identities = 24/71 (33%), Positives = 37/71 (52%), Gaps = 4/71 (5%) Frame = +3 Query: 612 FSTLGGSVXDRIAYRMFLDAXQKGILKPGKSVIVEPTSGNTGIGLALGR----CCXXVQV 779 F GGSV DR+A ++ +A + G L PG ++ E ++G+T I LA C V + Sbjct: 73 FLNPGGSVKDRVAVKIIQEALESGKLFPG-GIVTEGSAGSTAISLATVAPAYGCKCHVVI 131 Query: 780 PLXCCLRKCQM 812 P + K Q+ Sbjct: 132 PDDAAIEKSQI 142 >At5g35750.1 68418.m04281 histidine kinase (AHK2) identical to histidine kinase AHK2 [Arabidopsis thaliana] gi|13537196|dbj|BAB40774 Length = 1176 Score = 29.5 bits (63), Expect = 3.0 Identities = 13/29 (44%), Positives = 19/29 (65%) Frame = -3 Query: 509 VPSDFDPVPLQCEACPLV*RIFSQSSMCH 423 VP +FDP P+Q E P+ IF+Q ++ H Sbjct: 365 VPENFDPAPIQDEYAPV---IFAQETVSH 390 >At3g60400.1 68416.m06755 mitochondrial transcription termination factor-related / mTERF-related contains Pfam profile PF02536: mTERF Length = 558 Score = 29.1 bits (62), Expect = 3.9 Identities = 17/59 (28%), Positives = 32/59 (54%) Frame = +2 Query: 458 LKDMPHIVRALDRNQKVHSDILNVIGNTPLVKLSKLPKDEGLKCEMYAKCEFLNPGRIR 634 LK++P+++RA+D ++++ DIL + L + + DE L+ E E L R + Sbjct: 336 LKNLPYVLRAIDLHERIF-DILKNGNHHLLASYTLMDPDEDLEREYQEGLEELQNSRTK 393 >At3g14980.1 68416.m01894 PHD finger transcription factor, putative contains Pfam profile: PF00628 PHD-finger Length = 1189 Score = 27.9 bits (59), Expect = 9.0 Identities = 11/33 (33%), Positives = 18/33 (54%) Frame = +2 Query: 422 NGTSNSARKFFTLKDMPHIVRALDRNQKVHSDI 520 N S T+ + H+V +D NQ++HSD+ Sbjct: 389 NLISGCPESVLTVSEGSHLVHDVDANQEIHSDL 421 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,026,285 Number of Sequences: 28952 Number of extensions: 341265 Number of successful extensions: 704 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 674 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 702 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1970388800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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