Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_O16
(863 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase / 3-... 88 6e-18
At3g19480.1 68416.m02469 D-3-phosphoglycerate dehydrogenase, put... 82 4e-16
At4g34200.1 68417.m04854 D-3-phosphoglycerate dehydrogenase, put... 80 2e-15
At1g68010.1 68414.m07769 glycerate dehydrogenase / NADH-dependen... 41 0.001
At1g72190.1 68414.m08347 oxidoreductase family protein similar t... 39 0.004
At3g28790.1 68416.m03593 expressed protein 34 0.11
At1g79870.1 68414.m09330 oxidoreductase family protein contains ... 34 0.11
At1g15290.1 68414.m01830 tetratricopeptide repeat (TPR)-containi... 31 1.3
At1g65960.1 68414.m07484 glutamate decarboxylase 2 (GAD 2) simil... 30 2.3
At5g16930.1 68418.m01984 AAA-type ATPase family protein contains... 29 4.0
At2g45630.2 68415.m05674 oxidoreductase family protein low simil... 29 4.0
At1g68720.1 68414.m07851 cytidine/deoxycytidylate deaminase fami... 29 4.0
At1g49870.1 68414.m05591 expressed protein ; expression supporte... 29 4.0
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 29 5.3
At1g01390.1 68414.m00054 UDP-glucoronosyl/UDP-glucosyl transfera... 28 7.0
At3g56590.1 68416.m06293 hydroxyproline-rich glycoprotein family... 28 9.2
>At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase /
3-PGDH identical to SP|O04130
Length = 624
Score = 88.2 bits (209), Expect = 6e-18
Identities = 47/116 (40%), Positives = 73/116 (62%), Gaps = 1/116 (0%)
Frame = +1
Query: 139 VLIVDGVGAKCAELLNAYGIATTTKAKISKEELLMEIPNHDALVVRSATQVTKEVLDAGV 318
+L+ + +G LL +G + +S E+L ++ DAL+VRS T+VT+EV +A
Sbjct: 85 ILVTEKLGEAGVNLLREFGDVDCSY-DLSPEDLKKKVAESDALIVRSGTKVTREVFEAAK 143
Query: 319 -KLKVVGRAGAGVDNIDVXSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVQ 483
+LKVVGRAG G+DN+D+ +A + G V+NAP AN ++A E L+ +AR+V Q
Sbjct: 144 GRLKVVGRAGVGIDNVDLQAATEHGCLVVNAPTANTVAAAEHGIALLASMARNVAQ 199
Score = 37.5 bits (83), Expect = 0.011
Identities = 21/84 (25%), Positives = 34/84 (40%)
Frame = +3
Query: 516 RALYTGSELAGXXLXILGLGRVGXXVATXMYAFXMNIIGFQPFRVC*SVRPVPLHXXGAG 695
R+ Y G L G L ++G G+VG VA M +I P+ R + +
Sbjct: 211 RSKYVGVSLVGKTLAVMGFGKVGTEVARRAKGLGMTVISHDPYAPADRARALGVDLVSFD 270
Query: 696 GXFGPLTDXITLHTPVIEXTXXLY 767
D ++LH P+ T ++
Sbjct: 271 QAIS-TADFVSLHMPLTPATKKVF 293
>At3g19480.1 68416.m02469 D-3-phosphoglycerate dehydrogenase,
putative / 3-PGDH, putative similar to SP:O04130 from
[Arabidopsis thaliana]
Length = 588
Score = 82.2 bits (194), Expect = 4e-16
Identities = 45/117 (38%), Positives = 73/117 (62%), Gaps = 1/117 (0%)
Frame = +1
Query: 136 SVLIVDGVGAKCAELLNAYGIATTTKAKISKEELLMEIPNHDALVVRSATQVTKEVLDAG 315
++L+ + +G +LL Y + +S EEL +I DAL+VRS T+V ++V ++
Sbjct: 48 TILVTEKLGQAGIDLLKKYANVDCSY-DLSLEELCTKISLCDALIVRSGTKVGRDVFESS 106
Query: 316 V-KLKVVGRAGAGVDNIDVXSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVQ 483
+LKVVGRAG G+DN+D+ +A + G V+NAP AN ++A E L+ +AR++ Q
Sbjct: 107 RGRLKVVGRAGVGIDNVDLAAATEYGCLVVNAPTANTVAAAEHGIALLTAMARNIAQ 163
Score = 36.7 bits (81), Expect = 0.020
Identities = 23/80 (28%), Positives = 32/80 (40%)
Frame = +3
Query: 516 RALYTGSELAGXXLXILGLGRVGXXVATXMYAFXMNIIGFQPFRVC*SVRPVPLHXXGAG 695
R Y G L G L +LG G+VG VA M++I P+ R + +
Sbjct: 175 RNKYVGVSLVGKTLAVLGFGKVGSEVARRARGLGMHVITHDPYAPADRARAIGVELVSFE 234
Query: 696 GXFGPLTDXITLHTPVIEXT 755
D I+LH P+ T
Sbjct: 235 VAIS-TADFISLHLPLTAAT 253
>At4g34200.1 68417.m04854 D-3-phosphoglycerate dehydrogenase,
putative / 3-PGDH, putative similar to phosphoglycerate
dehydrogenase, Arabidopsis thaliana, SP:O04130
Length = 603
Score = 79.8 bits (188), Expect = 2e-15
Identities = 42/89 (47%), Positives = 62/89 (69%), Gaps = 1/89 (1%)
Frame = +1
Query: 220 ISKEELLMEIPNHDALVVRSATQVTKEVLDAGV-KLKVVGRAGAGVDNIDVXSAGKKGVG 396
++ EEL ++I DAL+VRS T+V +EV ++ +LKVVGRAG G+DN+D+ +A + G
Sbjct: 90 MTPEELNIKISLCDALIVRSGTKVGREVFESSHGRLKVVGRAGVGIDNVDLSAATEFGCL 149
Query: 397 VINAPGANALSACELTCTLMLVLARHVVQ 483
V+NAP AN ++A E LM +AR+V Q
Sbjct: 150 VVNAPTANTIAAAEHGIALMAAMARNVAQ 178
Score = 35.5 bits (78), Expect = 0.046
Identities = 22/80 (27%), Positives = 30/80 (37%)
Frame = +3
Query: 516 RALYTGSELAGXXLXILGLGRVGXXVATXMYAFXMNIIGFQPFRVC*SVRPVPLHXXGAG 695
R Y G L G L +LG G+VG VA M +I P+ + +
Sbjct: 190 RNKYVGVSLVGKTLAVLGFGKVGTEVARRAKGLGMRVIAHDPYAPADRAHAIGVDLVSFD 249
Query: 696 GXFGPLTDXITLHTPVIEXT 755
D I+LH P+ T
Sbjct: 250 EALA-TADFISLHMPLTPTT 268
>At1g68010.1 68414.m07769 glycerate dehydrogenase / NADH-dependent
hydroxypyruvate reductase identical to hydroxypyruvate
reductase (HPR) GB:D85339 [Arabidopsis thaliana] (Plant
Cell Physiol 1997 Apr;38(4):449-55)
Length = 386
Score = 41.1 bits (92), Expect = 0.001
Identities = 19/53 (35%), Positives = 27/53 (50%)
Frame = +1
Query: 325 KVVGRAGAGVDNIDVXSAGKKGVGVINAPGANALSACELTCTLMLVLARHVVQ 483
K G +N+DV +A K G+ V N PG + EL +L L AR +V+
Sbjct: 88 KAFSNMAVGYNNVDVEAANKYGIAVGNTPGVLTETTAELAASLSLAAARRIVE 140
>At1g72190.1 68414.m08347 oxidoreductase family protein similar to
D-3-phosphoglycerate dehydrogenase from Arabidopsis
thaliana [SP|O04130], glyoxylate reductase from Homo
sapiens (gi:6002730); contains Pfam D-isomer specific
2-hydroxyacid dehydrogenase, NAD binding domain PF02826
Length = 373
Score = 39.1 bits (87), Expect = 0.004
Identities = 22/79 (27%), Positives = 38/79 (48%), Gaps = 3/79 (3%)
Frame = +1
Query: 244 EIPNHDALVVRSATQVTKEVLDAGVKLKVVGRAGAGVDNIDVXSAGKKGVGVINAPG--- 414
E+ + + V Q+ V+ +K++ + G G+D +D+ +A K G+ V P
Sbjct: 90 EVIKNYHICVAMTMQMDSNVISRASNIKLIMQYGVGLDGVDIDAATKHGIKVARIPSEGT 149
Query: 415 ANALSACELTCTLMLVLAR 471
NA S E+ LML L +
Sbjct: 150 GNAASCSEMAIYLMLGLLK 168
>At3g28790.1 68416.m03593 expressed protein
Length = 608
Score = 34.3 bits (75), Expect = 0.11
Identities = 16/40 (40%), Positives = 21/40 (52%)
Frame = -1
Query: 425 SALAPGALITPTPFLPAEXTSMLSTPAPARPTTFSFTPAS 306
S +P TPTP P T STP P+ PT + TP++
Sbjct: 271 SGASPSGSPTPTPSTPTPSTPTPSTPTPSTPTPSTPTPST 310
Score = 33.5 bits (73), Expect = 0.19
Identities = 29/133 (21%), Positives = 50/133 (37%)
Frame = -1
Query: 656 SANTKGLKSDDVHAEGVHXSGHXXADPAKAKDGEXXPSELAASVQSPXPTCQXSAQ*SWT 477
S+++ S + ++G + + +E + S T + S S
Sbjct: 202 SSSSDNESSSNTKSQGTSSKSGSESTAGSIETNTGSKTEAGSKSSSSAKTKEVSGGSSGN 261
Query: 476 TWRARTNINVQVSSHALSALAPGALITPTPFLPAEXTSMLSTPAPARPTTFSFTPASNTS 297
T++ T + S P TPTP P T STP P+ PT TPA +T
Sbjct: 262 TYKDTTGSSSGASPSGSPTPTPS---TPTPSTPTPSTPTPSTPTPSTPT--PSTPAPSTP 316
Query: 296 LVT*VAERTTRAS 258
+E+ + ++
Sbjct: 317 AAGKTSEKGSESA 329
>At1g79870.1 68414.m09330 oxidoreductase family protein contains
Pfam profile: PF02826 D-isomer specific 2-hydroxyacid
dehydrogenase, NAD binding domain; similar to glyoxylate
reductase from Thermococcus litoralis [gi:13515409]
Length = 313
Score = 34.3 bits (75), Expect = 0.11
Identities = 18/74 (24%), Positives = 34/74 (45%)
Frame = +1
Query: 262 ALVVRSATQVTKEVLDAGVKLKVVGRAGAGVDNIDVXSAGKKGVGVINAPGANALSACEL 441
A+V ++ +++ L++V G+D ID+ +KG+ V N P +L
Sbjct: 48 AVVGNASAGADAQLISDLPNLEIVSSFSVGLDKIDLGKCKEKGIRVTNTPDVLTEDVADL 107
Query: 442 TCTLMLVLARHVVQ 483
L+L L R + +
Sbjct: 108 AIGLILALLRRLCE 121
>At1g15290.1 68414.m01830 tetratricopeptide repeat (TPR)-containing
protein ESTs gb|F20110 and gb|F20109 come from this gene;
contains Pfam profile PF00515: TPR Domain
Length = 1558
Score = 30.7 bits (66), Expect = 1.3
Identities = 18/52 (34%), Positives = 26/52 (50%), Gaps = 1/52 (1%)
Frame = +3
Query: 138 GFDRRRGWRQVCRTPQR-LRNRHHHQGQDLQGRTSYGDTQPRRSGCAFSNSS 290
G R+ RQ +R L N+HH++ QD+Q + Y Q G + S SS
Sbjct: 1165 GAGRKSRQRQPDLMKKRMLLNKHHNRNQDVQQQNIYSPLQKTSKGPSLSKSS 1216
>At1g65960.1 68414.m07484 glutamate decarboxylase 2 (GAD 2) similar
to glutamate decarboxylase (gad) GI:294111 from [Petunia
hybrida]
Length = 494
Score = 29.9 bits (64), Expect = 2.3
Identities = 16/46 (34%), Positives = 28/46 (60%), Gaps = 1/46 (2%)
Frame = +1
Query: 115 KMVVDIKSVLI-VDGVGAKCAELLNAYGIATTTKAKISKEELLMEI 249
++V DI VL +D + +K ++ + GIA K K ++E+LME+
Sbjct: 431 RLVADISKVLHELDTLPSKISKKMGIEGIAENVKEKKMEKEILMEV 476
>At5g16930.1 68418.m01984 AAA-type ATPase family protein contains
Pfam domain, PF00004: ATPase, AAA family
Length = 644
Score = 29.1 bits (62), Expect = 4.0
Identities = 17/50 (34%), Positives = 24/50 (48%), Gaps = 3/50 (6%)
Frame = -2
Query: 547 PASSLPVYRARXPPASXQRSE---AGPRGEPEPTSTCRSARTRSAHWRPE 407
P++S+P + PPAS E AG + P + RT SA + PE
Sbjct: 40 PSASIPQQQGSTPPASESGKEPSVAGEESDAPPRIRNNNPRTTSAGFDPE 89
>At2g45630.2 68415.m05674 oxidoreductase family protein low
similarity to SP|P36234 Glycerate dehydrogenase (EC
1.1.1.29) (NADH-dependent hydroxypyruvate reductase)
{Hyphomicrobium methylovorum}; contains Pfam profile
PF00389: D-isomer specific 2-hydroxyacid dehydrogenase,
catalytic domain
Length = 338
Score = 29.1 bits (62), Expect = 4.0
Identities = 14/32 (43%), Positives = 19/32 (59%)
Frame = +3
Query: 531 GSELAGXXLXILGLGRVGXXVATXMYAFXMNI 626
GS+L + I+GLG +G VAT + AF I
Sbjct: 163 GSKLGRKRIGIVGLGSIGSKVATRLDAFGCQI 194
>At1g68720.1 68414.m07851 cytidine/deoxycytidylate deaminase family
protein contains Pfam profile PF00383: Cytidine and
deoxycytidylate deaminase zinc-binding region
Length = 1307
Score = 29.1 bits (62), Expect = 4.0
Identities = 20/63 (31%), Positives = 28/63 (44%)
Frame = -2
Query: 673 GTGRTDQQTRKG*NPMMFMXKAYIXVATXXPTLPRPRMXRXCPASSLPVYRARXPPASXQ 494
G GR + G NP++F + A P +PRPRM +SSL + P S +
Sbjct: 1006 GEGRRYPRMDAGQNPLLFPGRNLRSPAVMEPPVPRPRMVSG--SSSLREQVEQQQPLSAK 1063
Query: 493 RSE 485
E
Sbjct: 1064 SQE 1066
>At1g49870.1 68414.m05591 expressed protein ; expression supported
by MPSS
Length = 828
Score = 29.1 bits (62), Expect = 4.0
Identities = 18/72 (25%), Positives = 39/72 (54%), Gaps = 3/72 (4%)
Frame = +1
Query: 121 VVDIKSVLI---VDGVGAKCAELLNAYGIATTTKAKISKEELLMEIPNHDALVVRSATQV 291
+ D++S+L+ V G+ + E + G K +++ L E+P+ D+ +V+ +++
Sbjct: 480 IPDLESLLVKKHVSGLEKEVQETIKNCGKMYENVKKPGRKDGLSEVPSLDSCLVKHFSKL 539
Query: 292 TKEVLDAGVKLK 327
KEV +A + K
Sbjct: 540 EKEVQEAKKRSK 551
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 28.7 bits (61), Expect = 5.3
Identities = 24/71 (33%), Positives = 32/71 (45%), Gaps = 1/71 (1%)
Frame = +3
Query: 54 PDARRRRTPASSLTTIALPNQDGRGH*VGFDRRRGWRQVCRTPQRLRNRHHH-QGQDLQG 230
PD RRRR+P+SS + + H R R ++ R+P RL Q L
Sbjct: 603 PDRRRRRSPSSSRSP-SRSRSPPVLHRSPSPRGRKHQRERRSPGRLSEEQDRVQNSKLLK 661
Query: 231 RTSYGDTQPRR 263
RTS DT R+
Sbjct: 662 RTSVPDTDKRK 672
Score = 27.9 bits (59), Expect = 9.2
Identities = 24/95 (25%), Positives = 34/95 (35%), Gaps = 1/95 (1%)
Frame = -2
Query: 574 PRPRMXRXCPASSLPVYRARXPPASXQRSEAGP-RGEPEPTSTCRSARTRSAHWRPEXXX 398
P P R P+ R+ PPA +RS + P R PT R R+ S R
Sbjct: 304 PAPSRRRRSPSPPARRRRSPSPPARRRRSPSPPARRHRSPTPPARQRRSPSPPARRHRSP 363
Query: 397 XXXXXXXXXXXRCCPHQLQHDRPPSASRLRPTPLW 293
+ + PP+ R P+PL+
Sbjct: 364 PPARRRRSPSPPA--RRRRSPSPPARRRRSPSPLY 396
>At1g01390.1 68414.m00054 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 480
Score = 28.3 bits (60), Expect = 7.0
Identities = 14/40 (35%), Positives = 18/40 (45%)
Frame = -1
Query: 416 APGALITPTPFLPAEXTSMLSTPAPARPTTFSFTPASNTS 297
A G L+ L + L PAP +PT + P NTS
Sbjct: 207 AKGILVNSFVDLESNAIKALQEPAPDKPTVYPIGPLVNTS 246
>At3g56590.1 68416.m06293 hydroxyproline-rich glycoprotein family
protein
Length = 477
Score = 27.9 bits (59), Expect = 9.2
Identities = 21/51 (41%), Positives = 24/51 (47%), Gaps = 5/51 (9%)
Frame = -2
Query: 574 PRPRMXRXCPASS-LP-VYRARXPPASXQRSEAGPRGE--PEPTST-CRSA 437
P P P SS LP V A PP S E+ P GE P P T C+S+
Sbjct: 415 PAPPRHHAIPVSSPLPHVVFAHIPPPSKSSPESEPTGEKSPSPAPTPCKSS 465
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,281,261
Number of Sequences: 28952
Number of extensions: 277450
Number of successful extensions: 854
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 766
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 838
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2019160800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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