BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP03_F_O01 (904 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 36 0.010 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 31 0.17 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 31 0.22 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 30 0.52 SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 26 6.4 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 23 7.0 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 35.5 bits (78), Expect = 0.010 Identities = 21/62 (33%), Positives = 24/62 (38%) Frame = +2 Query: 494 KKKXXXXXPPPPPPXXXXXXXXPGAXAPLFXXGXXPXPXPFXXKXPXXPPPPXXXPAPXP 673 ++K PPPPPP P AP+ P P P P PPPP A P Sbjct: 724 QQKLLLKSPPPPPPAVIVPTPAP---API----PVPPPAPIMGGPPPPPPPPGVAGAGPP 776 Query: 674 XP 679 P Sbjct: 777 PP 778 Score = 26.2 bits (55), Expect = 6.4 Identities = 15/55 (27%), Positives = 17/55 (30%) Frame = +1 Query: 520 PPPPPPXXLXXXXXXXXXXXXXXXXXPXXXSLXXXXXXTPPPPXXXPXPPPAXPP 684 PPPPPP + P + PPP PPP PP Sbjct: 732 PPPPPPAVIVPTPAPAPIPVP-----PPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 31.5 bits (68), Expect = 0.17 Identities = 20/64 (31%), Positives = 20/64 (31%), Gaps = 2/64 (3%) Frame = +2 Query: 488 PPKKKXXXXXPPPPPPXXXXXXXXPGAXAP--LFXXGXXPXPXPFXXKXPXXPPPPXXXP 661 PP K PP P P P AP G P P P P P P P Sbjct: 1102 PPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKP-SVAAPPVPAPSGAPP 1160 Query: 662 APXP 673 P P Sbjct: 1161 VPKP 1164 Score = 31.5 bits (68), Expect = 0.17 Identities = 19/64 (29%), Positives = 19/64 (29%), Gaps = 2/64 (3%) Frame = +2 Query: 488 PPKKKXXXXXPPPPPPXXXXXXXXPGAXAPLF--XXGXXPXPXPFXXKXPXXPPPPXXXP 661 PP K PP P P P AP G P P P P P P Sbjct: 1121 PPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPP 1180 Query: 662 APXP 673 P P Sbjct: 1181 VPKP 1184 Score = 29.9 bits (64), Expect = 0.52 Identities = 21/64 (32%), Positives = 21/64 (32%) Frame = +2 Query: 488 PPKKKXXXXXPPPPPPXXXXXXXXPGAXAPLFXXGXXPXPXPFXXKXPXXPPPPXXXPAP 667 PP K P PPP P AP G P P P P P P P P Sbjct: 1050 PPVPKSSSGAPSAPPPVPAPSSEIPSIPAP---SGAPPVPAP--SGIPPVPKPSVAAP-P 1103 Query: 668 XPXP 679 P P Sbjct: 1104 VPKP 1107 Score = 29.5 bits (63), Expect = 0.68 Identities = 18/64 (28%), Positives = 18/64 (28%), Gaps = 2/64 (3%) Frame = +2 Query: 488 PPKKKXXXXXPPPPPPXXXXXXXXPGAXAPLF--XXGXXPXPXPFXXKXPXXPPPPXXXP 661 PP K PP PPP P P P P P P P P Sbjct: 1179 PPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPP 1238 Query: 662 APXP 673 P P Sbjct: 1239 VPAP 1242 Score = 27.1 bits (57), Expect = 3.6 Identities = 18/65 (27%), Positives = 19/65 (29%), Gaps = 1/65 (1%) Frame = +2 Query: 488 PPKKKXXXXXPP-PPPPXXXXXXXXPGAXAPLFXXGXXPXPXPFXXKXPXXPPPPXXXPA 664 PP K PP P P P P+ P P P P P P Sbjct: 1092 PPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAP- 1150 Query: 665 PXPXP 679 P P P Sbjct: 1151 PVPAP 1155 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 31.1 bits (67), Expect = 0.22 Identities = 17/54 (31%), Positives = 17/54 (31%) Frame = +2 Query: 518 PPPPPPXXXXXXXXPGAXAPLFXXGXXPXPXPFXXKXPXXPPPPXXXPAPXPXP 679 PPPPPP P P P P P PP AP P P Sbjct: 312 PPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPP 365 Score = 29.5 bits (63), Expect = 0.68 Identities = 20/66 (30%), Positives = 20/66 (30%), Gaps = 2/66 (3%) Frame = +2 Query: 488 PPKKKXXXXXPPPPP--PXXXXXXXXPGAXAPLFXXGXXPXPXPFXXKXPXXPPPPXXXP 661 PP P PP P GA A P P P PP P P Sbjct: 415 PPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAP 474 Query: 662 APXPXP 679 AP P P Sbjct: 475 APPPAP 480 Score = 25.8 bits (54), Expect = 8.4 Identities = 15/55 (27%), Positives = 15/55 (27%) Frame = +1 Query: 520 PPPPPPXXLXXXXXXXXXXXXXXXXXPXXXSLXXXXXXTPPPPXXXPXPPPAXPP 684 PP PP P L PP P P PPPA P Sbjct: 428 PPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAP 482 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 29.9 bits (64), Expect = 0.52 Identities = 11/23 (47%), Positives = 12/23 (52%) Frame = +1 Query: 634 TPPPPXXXPXPPPAXPPXXXXXP 702 TPPPP PPP+ PP P Sbjct: 1706 TPPPPPMSVPPPPSAPPMPAGPP 1728 >SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 1420 Score = 26.2 bits (55), Expect = 6.4 Identities = 17/59 (28%), Positives = 17/59 (28%), Gaps = 4/59 (6%) Frame = +1 Query: 520 PPPPPPXXLXXXXXXXXXXXXXXXXXPXXXSLXXXXXXTP----PPPXXXPXPPPAXPP 684 PPPPPP S TP P P P PPP PP Sbjct: 190 PPPPPPPPAVEDQAADANEPDDYYSSGRAVSPEIPPTYTPKQADPLPAPPPPPPPTLPP 248 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 23.0 bits (47), Expect(2) = 7.0 Identities = 8/17 (47%), Positives = 8/17 (47%) Frame = +2 Query: 629 PXXPPPPXXXPAPXPXP 679 P PPPP P P P Sbjct: 10 PPPPPPPGFEPPSQPPP 26 Score = 21.0 bits (42), Expect(2) = 7.0 Identities = 6/6 (100%), Positives = 6/6 (100%) Frame = +2 Query: 518 PPPPPP 535 PPPPPP Sbjct: 9 PPPPPP 14 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.315 0.154 0.546 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,631,957 Number of Sequences: 5004 Number of extensions: 23706 Number of successful extensions: 213 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 20 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 112 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 456499320 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 42 (21.8 bits)
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