BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP03_F_N13 (956 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 34 0.003 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 36 0.006 SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 36 0.008 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 36 0.008 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 34 0.026 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 34 0.034 SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 32 0.10 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 31 0.18 SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pomb... 29 1.3 SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 22 7.9 SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizos... 26 9.0 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 34.3 bits (75), Expect(2) = 0.003 Identities = 12/22 (54%), Positives = 12/22 (54%) Frame = +2 Query: 359 PPPPXPXPAXXPPPXAPXPXPP 424 PPPP P P PP P P PP Sbjct: 9 PPPPPPPPGFEPPSQPPPPPPP 30 Score = 33.9 bits (74), Expect = 0.034 Identities = 12/22 (54%), Positives = 12/22 (54%) Frame = +2 Query: 362 PPPXPXPAXXPPPXAPXPXPPP 427 PPP P P PP P P PPP Sbjct: 9 PPPPPPPPGFEPPSQPPPPPPP 30 Score = 31.5 bits (68), Expect = 0.18 Identities = 12/26 (46%), Positives = 12/26 (46%) Frame = +2 Query: 362 PPPXPXPAXXPPPXAPXPXPPPXXPP 439 PP P P PP P PPP PP Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPP 30 Score = 21.8 bits (44), Expect(2) = 0.003 Identities = 7/11 (63%), Positives = 7/11 (63%) Frame = +2 Query: 245 PXPXPPXPPPP 277 P PP PPPP Sbjct: 5 PPGNPPPPPPP 15 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 36.3 bits (80), Expect = 0.006 Identities = 24/69 (34%), Positives = 24/69 (34%), Gaps = 4/69 (5%) Frame = +2 Query: 245 PXPXPPX-PP--PPXXXPXXXXXXXXXXXAXXXXXXXXXXXPPPPXPXPAXXP-PPXAPX 412 P P PP PP PP P A PP P PA P PP AP Sbjct: 416 PVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPA 475 Query: 413 PXPPPXXPP 439 P P P P Sbjct: 476 PPPAPAPAP 484 Score = 31.1 bits (67), Expect = 0.24 Identities = 17/46 (36%), Positives = 18/46 (39%), Gaps = 3/46 (6%) Frame = +2 Query: 359 PPPPXPXPAXXPPPXAP---XPXPPPXXPPXXXXXGXXXGXPGLPP 487 PP P P+ PP P P PPP PP G P PP Sbjct: 230 PPIPPSIPSSRPPERVPSLSAPAPPPIPPP---SNGTVSSPPNSPP 272 Score = 29.1 bits (62), Expect = 0.97 Identities = 24/96 (25%), Positives = 26/96 (27%), Gaps = 4/96 (4%) Frame = +2 Query: 257 PPXPPP--PXXXPXXXXXXXXXXXAXXXXXXXXXXXPP--PPXPXPAXXPPPXAPXPXPP 424 PP PPP P PP PP P+ P P P Sbjct: 388 PPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAP 447 Query: 425 PXXPPXXXXXGXXXGXPGLPPNRGIPXXLXRXPPXP 532 P P G P PP +P PP P Sbjct: 448 PLPPSAPIAPPLPAGMPAAPP---LPPAAPAPPPAP 480 Score = 26.2 bits (55), Expect = 6.8 Identities = 13/42 (30%), Positives = 14/42 (33%) Frame = +2 Query: 362 PPPXPXPAXXPPPXAPXPXPPPXXPPXXXXXGXXXGXPGLPP 487 PPP P P+ P P P P P LPP Sbjct: 253 PPPIPPPSNGTVSSPPNSPPRPIAPVSMNPAINSTSKPPLPP 294 Score = 25.8 bits (54), Expect = 9.0 Identities = 23/100 (23%), Positives = 25/100 (25%), Gaps = 9/100 (9%) Frame = +2 Query: 260 PXPPPPXXXPXXXXXXXXXXXAXXXXXXXXXXXPPPPXPXPAXXP---------PPXAPX 412 P PPPP P + P PA P PP Sbjct: 361 PPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTP 420 Query: 413 PXPPPXXPPXXXXXGXXXGXPGLPPNRGIPXXLXRXPPXP 532 P PP PP G P +P PP P Sbjct: 421 PSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLP 460 >SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Schizosaccharomyces pombe|chr 1|||Manual Length = 1995 Score = 35.9 bits (79), Expect = 0.008 Identities = 17/42 (40%), Positives = 18/42 (42%) Frame = +2 Query: 359 PPPPXPXPAXXPPPXAPXPXPPPXXPPXXXXXGXXXGXPGLP 484 PPPP P PP AP PP PP G PG+P Sbjct: 1884 PPPPMALPKAGPPSAAPTSALPPAGPPAGAT--AISGNPGMP 1923 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 35.9 bits (79), Expect = 0.008 Identities = 28/104 (26%), Positives = 31/104 (29%), Gaps = 8/104 (7%) Frame = +2 Query: 245 PXPXPPXPPPPXXXPXXXXXXXXXXXAXXXXXXXXXXXPP--------PPXPXPAXXPPP 400 P P P PP P A PP PP P P+ PP Sbjct: 1141 PVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPV 1200 Query: 401 XAPXPXPPPXXPPXXXXXGXXXGXPGLPPNRGIPXXLXRXPPXP 532 P PP PP GLPP +P + PP P Sbjct: 1201 PKPSVGVPPVPPPSTAPP-VPTPSAGLPP---VPVPTAKAPPVP 1240 Score = 31.1 bits (67), Expect = 0.24 Identities = 24/103 (23%), Positives = 27/103 (26%), Gaps = 7/103 (6%) Frame = +2 Query: 245 PXPXPPXPPPPXXXPXXXXXXXXXXXAXXXXXXXXXXXPP-------PPXPXPAXXPPPX 403 P P P PP P PP PP P P+ PP Sbjct: 1093 PVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPV 1152 Query: 404 APXPXPPPXXPPXXXXXGXXXGXPGLPPNRGIPXXLXRXPPXP 532 PP P G+PP +P PP P Sbjct: 1153 PAPSGAPPVPKPSVAAPPVPAPSSGIPP---VPKPAAGVPPVP 1192 Score = 29.1 bits (62), Expect = 0.97 Identities = 19/66 (28%), Positives = 22/66 (33%) Frame = +2 Query: 365 PPXPXPAXXPPPXAPXPXPPPXXPPXXXXXGXXXGXPGLPPNRGIPXXLXRXPPXP*IXG 544 PP P P+ PP P PP P G PP +P PP P + Sbjct: 1083 PPVPAPSGIPPVPKPSVAAPPVPKPSVAVP-PVPAPSGAPP---VPKPSVAAPPVP-VPS 1137 Query: 545 GGKTXP 562 G P Sbjct: 1138 GAPPVP 1143 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 34.3 bits (75), Expect = 0.026 Identities = 18/48 (37%), Positives = 18/48 (37%), Gaps = 5/48 (10%) Frame = +2 Query: 359 PPPPXPXPAXXPPPXAPXPXPPP-----XXPPXXXXXGXXXGXPGLPP 487 PPPP P P AP P PPP PP G P PP Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPP 779 Score = 33.9 bits (74), Expect = 0.034 Identities = 17/42 (40%), Positives = 17/42 (40%) Frame = +2 Query: 362 PPPXPXPAXXPPPXAPXPXPPPXXPPXXXXXGXXXGXPGLPP 487 P P P P PPP AP PP PP G P PP Sbjct: 742 PTPAPAPIPVPPP-APIMGGPPPPPPPPGVAGAGPPPPPPPP 782 Score = 30.7 bits (66), Expect = 0.32 Identities = 15/38 (39%), Positives = 15/38 (39%), Gaps = 6/38 (15%) Frame = +2 Query: 362 PPPXPXPAXXPPPXAPX------PXPPPXXPPXXXXXG 457 PPP P PPP P P PPP PP G Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGG 789 Score = 30.3 bits (65), Expect = 0.42 Identities = 20/49 (40%), Positives = 20/49 (40%), Gaps = 7/49 (14%) Frame = +2 Query: 359 PPP----PXPXPAXXP-PPXAPXPX--PPPXXPPXXXXXGXXXGXPGLP 484 PPP P P PA P PP AP PPP PP G P P Sbjct: 735 PPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783 Score = 29.1 bits (62), Expect = 0.97 Identities = 15/47 (31%), Positives = 16/47 (34%) Frame = +2 Query: 392 PPPXAPXPXPPPXXPPXXXXXGXXXGXPGLPPNRGIPXXLXRXPPXP 532 PPP P P P P G P PP G+ PP P Sbjct: 735 PPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781 Score = 27.9 bits (59), Expect = 2.2 Identities = 14/47 (29%), Positives = 15/47 (31%) Frame = +3 Query: 423 PPXPPPXXXXXXRXXAXXXSPPTGESLXXXSXXPXTPKXWGGEKPPP 563 PP PPP A PP + P P G PPP Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPP 778 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 33.9 bits (74), Expect = 0.034 Identities = 17/43 (39%), Positives = 17/43 (39%), Gaps = 1/43 (2%) Frame = +2 Query: 362 PPPXPXPAXXPPPXAPXPXPPP-XXPPXXXXXGXXXGXPGLPP 487 PP P A P AP P PPP PP G P PP Sbjct: 1690 PPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPP 1732 Score = 32.7 bits (71), Expect = 0.079 Identities = 14/28 (50%), Positives = 15/28 (53%), Gaps = 1/28 (3%) Frame = +2 Query: 359 PPPPXPXPAXXPPPXAPX-PXPPPXXPP 439 P PP P + PPP AP P PP PP Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAPP 1732 Score = 29.9 bits (64), Expect = 0.55 Identities = 13/33 (39%), Positives = 13/33 (39%) Frame = +2 Query: 359 PPPPXPXPAXXPPPXAPXPXPPPXXPPXXXXXG 457 PPPP P PP AP P P P G Sbjct: 1715 PPPPSAPPMPAGPPSAPPPPLPASSAPSVPNPG 1747 >SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 1420 Score = 32.3 bits (70), Expect = 0.10 Identities = 20/62 (32%), Positives = 20/62 (32%), Gaps = 1/62 (1%) Frame = +2 Query: 257 PPXPPPPXXXPXXXXXXXXXXXAXXXXXXXXXXXPP-PPXPXPAXXPPPXAPXPXPPPXX 433 PP PPPP P P PP P P AP P PPP Sbjct: 189 PPPPPPPP--PAVEDQAADANEPDDYYSSGRAVSPEIPPTYTPKQADPLPAPPPPPPPTL 246 Query: 434 PP 439 PP Sbjct: 247 PP 248 Score = 31.9 bits (69), Expect = 0.14 Identities = 15/52 (28%), Positives = 17/52 (32%) Frame = +2 Query: 245 PXPXPPXPPPPXXXPXXXXXXXXXXXAXXXXXXXXXXXPPPPXPXPAXXPPP 400 P P PP PPPP P + PPP P+ P P Sbjct: 234 PLPAPPPPPPPTLPPQSTNTSQLPMPSRNVNNLGSQVNIPPPPATPSQPPRP 285 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 31.5 bits (68), Expect = 0.18 Identities = 17/59 (28%), Positives = 20/59 (33%), Gaps = 1/59 (1%) Frame = +2 Query: 359 PPPPXPXPAXXPPPXAPXPXPPPXXPPXXXXXGXXXGXPGLPP-NRGIPXXLXRXPPXP 532 P P P PA PP + P P PP P P +P + PP P Sbjct: 147 PSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPPPP 205 Score = 27.1 bits (57), Expect = 3.9 Identities = 12/22 (54%), Positives = 12/22 (54%) Frame = +2 Query: 359 PPPPXPXPAXXPPPXAPXPXPP 424 P PP P PA P P A P PP Sbjct: 3 PAPPPPPPA--PAPAAAAPAPP 22 Score = 25.8 bits (54), Expect = 9.0 Identities = 10/20 (50%), Positives = 10/20 (50%) Frame = +2 Query: 380 PAXXPPPXAPXPXPPPXXPP 439 PA PPP AP P PP Sbjct: 3 PAPPPPPPAPAPAAAAPAPP 22 Score = 25.8 bits (54), Expect = 9.0 Identities = 12/27 (44%), Positives = 12/27 (44%), Gaps = 1/27 (3%) Frame = +2 Query: 359 PPPPXPXPAXXPPPXA-PXPXPPPXXP 436 P PP P PP A P P PP P Sbjct: 128 PAPPTPQSELRPPTSAPPRPSIPPPSP 154 >SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 331 Score = 28.7 bits (61), Expect = 1.3 Identities = 17/49 (34%), Positives = 18/49 (36%), Gaps = 3/49 (6%) Frame = +2 Query: 365 PPXPXPAXXPPPXAPXPXPP-PXXPPXXXXXGXXXGXPGLP--PNRGIP 502 P P P P P P P P P PP PG P PN +P Sbjct: 99 PEEPLPREPPLPNEPVPEEPLPGEPPLPDEPVPEEPLPGEPPLPNEPVP 147 Score = 26.6 bits (56), Expect = 5.2 Identities = 12/28 (42%), Positives = 12/28 (42%), Gaps = 1/28 (3%) Frame = +2 Query: 359 PPPPXPXPAXXPPPXAPXPXPP-PXXPP 439 P P P P P P P P P P PP Sbjct: 113 PVPEEPLPGEPPLPDEPVPEEPLPGEPP 140 >SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2|||Manual Length = 1217 Score = 22.2 bits (45), Expect(2) = 7.9 Identities = 7/11 (63%), Positives = 7/11 (63%) Frame = +2 Query: 245 PXPXPPXPPPP 277 P PP PPPP Sbjct: 1091 PSAVPPPPPPP 1101 Score = 21.8 bits (44), Expect(2) = 7.9 Identities = 7/9 (77%), Positives = 7/9 (77%) Frame = +2 Query: 359 PPPPXPXPA 385 PPPP P PA Sbjct: 1095 PPPPPPPPA 1103 >SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizosaccharomyces pombe|chr 2|||Manual Length = 872 Score = 25.8 bits (54), Expect = 9.0 Identities = 12/29 (41%), Positives = 12/29 (41%), Gaps = 2/29 (6%) Frame = +2 Query: 359 PPPPXPXPAXXPP--PXAPXPXPPPXXPP 439 PPPP P P P P P P P P Sbjct: 358 PPPPMPAPIYNVPNVPTVPTVSPNPFLQP 386 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.317 0.159 0.615 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,445,864 Number of Sequences: 5004 Number of extensions: 21163 Number of successful extensions: 255 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 38 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 127 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 489310570 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 42 (21.8 bits)
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