BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP03_F_N08 (862 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 28 2.0 SPAC11H11.01 |sst6|cps23|ESCRT I complex subunit Vps23|Schizosac... 28 2.0 SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces ... 27 4.5 SPCC1450.12 |||conserved fungal protein|Schizosaccharomyces pomb... 26 6.0 SPAC1F12.06c |||endonuclease |Schizosaccharomyces pombe|chr 1|||... 26 7.9 SPBC28F2.02 |mep33||mRNA export protein Mep33|Schizosaccharomyce... 26 7.9 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 27.9 bits (59), Expect = 2.0 Identities = 11/20 (55%), Positives = 11/20 (55%) Frame = +3 Query: 801 PPPPPGGXGGXXFFRPPPXP 860 PPPPP G G PPP P Sbjct: 763 PPPPPPGVAGAGPPPPPPPP 782 Score = 27.5 bits (58), Expect = 2.6 Identities = 11/20 (55%), Positives = 11/20 (55%) Frame = +3 Query: 801 PPPPPGGXGGXXFFRPPPXP 860 PPPPPG G PPP P Sbjct: 764 PPPPPGVAGAGPPPPPPPPP 783 Score = 26.2 bits (55), Expect = 6.0 Identities = 11/26 (42%), Positives = 13/26 (50%) Frame = +3 Query: 783 SPL*XXPPPPPGGXGGXXFFRPPPXP 860 +P+ PPPPP G PPP P Sbjct: 755 APIMGGPPPPPPPPGVAGAGPPPPPP 780 >SPAC11H11.01 |sst6|cps23|ESCRT I complex subunit Vps23|Schizosaccharomyces pombe|chr 1|||Manual Length = 487 Score = 27.9 bits (59), Expect = 2.0 Identities = 16/49 (32%), Positives = 23/49 (46%) Frame = +2 Query: 143 VHAFVKRDAPKEDNSINTLAESAKKTIEELREKVESALAPETVKKNFGT 289 V A +K+D +E S L TIE+L+ K E+ P K F + Sbjct: 128 VQALIKQDFEREHTSPPELPTKLVNTIEKLKVKEENEAPPVIPAKPFSS 176 >SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 2344 Score = 26.6 bits (56), Expect = 4.5 Identities = 15/57 (26%), Positives = 29/57 (50%) Frame = -2 Query: 261 GAKADSTFSLNSSIVFFALSASVFMLLSSLGASRLTNACTLARQIANKIISSLFILD 91 G A + F ++ SIV+ A+ ++V + + A ++ NK+ S L++LD Sbjct: 410 GYMAITKFEVSQSIVYSAIVSAVAEFIRQILAEDQLLLNNFFEELKNKLESDLYLLD 466 >SPCC1450.12 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 821 Score = 26.2 bits (55), Expect = 6.0 Identities = 13/28 (46%), Positives = 18/28 (64%) Frame = +2 Query: 173 KEDNSINTLAESAKKTIEELREKVESAL 256 KE NSI+ L+ S +KTIE R + S + Sbjct: 371 KEKNSISELSPSLQKTIEWARVNLASTI 398 >SPAC1F12.06c |||endonuclease |Schizosaccharomyces pombe|chr 1|||Manual Length = 252 Score = 25.8 bits (54), Expect = 7.9 Identities = 16/75 (21%), Positives = 36/75 (48%) Frame = -1 Query: 427 YSITSRTLLHSIYLGLCT*ILKNYFSGFRCFRGLQIFIKFVEAVYHRAKVFLNSLRGQGG 248 Y + R +++ YL + + ++Y GF FR ++ ++ + + H+ ++ + + G G Sbjct: 60 YDLEQRMIIYKDYLCIEK-LEEDYVPGFLSFREIKWYLPLLNHIPHQFRIDIILVDGNGV 118 Query: 247 FNFFPQFLNCFLRTL 203 + L C L L Sbjct: 119 LHPVGFGLACHLGVL 133 >SPBC28F2.02 |mep33||mRNA export protein Mep33|Schizosaccharomyces pombe|chr 2|||Manual Length = 292 Score = 25.8 bits (54), Expect = 7.9 Identities = 16/39 (41%), Positives = 22/39 (56%), Gaps = 1/39 (2%) Frame = +1 Query: 277 KLWHDGRQLQRIL*KSEARGSTESL-RSNFLVSKYITPN 390 K W DGR+ RIL ++E R S ++ RSN +Y N Sbjct: 216 KKWSDGRR-DRILKQAEERRSNRAVGRSNLSGREYFESN 253 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,439,310 Number of Sequences: 5004 Number of extensions: 41035 Number of successful extensions: 148 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 138 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 146 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 428468660 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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