BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_N04
(915 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 33 0.043
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 31 0.23
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 29 1.2
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 2.1
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 27 4.9
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 4.9
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 26 8.6
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 33.5 bits (73), Expect = 0.043
Identities = 23/91 (25%), Positives = 25/91 (27%), Gaps = 2/91 (2%)
Frame = +1
Query: 643 PPXXXPXXXXPSXXPPXXXRXXXQXXXFXGXPXAXXPXPP--PXGTPPXXPAKTXXXPPP 816
PP P P+ PP P PP P PP P PP
Sbjct: 391 PPPAIPGRSAPAL-PPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPL 449
Query: 817 XPXPXXXPPXXGGSXXTXFSSXSXXXPPPXP 909
P PP G + PPP P
Sbjct: 450 PPSAPIAPPLPAGMPAAPPLPPAAPAPPPAP 480
Score = 31.5 bits (68), Expect = 0.17
Identities = 24/86 (27%), Positives = 28/86 (32%), Gaps = 5/86 (5%)
Frame = +1
Query: 673 PSXXPPXXXRXXXQXXXFXGXPXAXXPXPPPXGTPPXXPAKTXXXPP-----PXPXPXXX 837
P PP R + G + P PPP P A + PP P P P
Sbjct: 311 PPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPP--PRSNAAGSIPLPPQGRSAPPPPPPRS 368
Query: 838 PPXXGGSXXTXFSSXSXXXPPPXPXA 915
P G SS + PP P A
Sbjct: 369 APSTGRQPPPLSSSRAVSNPPAPPPA 394
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 31.1 bits (67), Expect = 0.23
Identities = 25/91 (27%), Positives = 26/91 (28%), Gaps = 2/91 (2%)
Frame = +1
Query: 643 PPXXXPXXXXPSXXPPXXXRXXXQXXX--FXGXPXAXXPXPPPXGTPPXXPAKTXXXPPP 816
P P PS PP G P A P P P P PA + PP
Sbjct: 1027 PSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPS--GAPP 1084
Query: 817 XPXPXXXPPXXGGSXXTXFSSXSXXXPPPXP 909
P P PP S PP P
Sbjct: 1085 VPAPSGIPPVPKPSVAAPPVPKPSVAVPPVP 1115
Score = 29.9 bits (64), Expect = 0.53
Identities = 33/131 (25%), Positives = 36/131 (27%), Gaps = 3/131 (2%)
Frame = +1
Query: 532 PXXPPPXXXXPXXXXXXPXXXFPXXXGXPLFSR-GFLXPPXXXPXXXXPSXXPPXXXRXX 708
P P P P P G P + PP P P P
Sbjct: 1064 PPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPV 1123
Query: 709 XQXXXFXGXPXAXXPXPPPXGTPPXXPAKTXXXPPPXPXPXXXPPXXGGSXXT-XFSSXS 885
+ A P P P G PP K PP P P PP S + S
Sbjct: 1124 PKPSV------AAPPVPVPSGAPPV--PKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPS 1175
Query: 886 XXXPP-PXPXA 915
PP P P A
Sbjct: 1176 SGIPPVPKPAA 1186
Score = 29.9 bits (64), Expect = 0.53
Identities = 30/130 (23%), Positives = 34/130 (26%), Gaps = 2/130 (1%)
Frame = +1
Query: 532 PXXPPPXXXXPXXXXXX--PXXXFPXXXGXPLFSRGFLXPPXXXPXXXXPSXXPPXXXRX 705
P P P P P P P+ + PP P P PP
Sbjct: 1140 PPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPP 1199
Query: 706 XXQXXXFXGXPXAXXPXPPPXGTPPXXPAKTXXXPPPXPXPXXXPPXXGGSXXTXFSSXS 885
+ G P P PPP PP P P P P S S+
Sbjct: 1200 VPKPSV--GVP----PVPPPSTAPPVPTPSAGLPPVPVPTAKAPPVPAPSSEAPSVSTPR 1253
Query: 886 XXXPPPXPXA 915
P P A
Sbjct: 1254 SSVPSPHSNA 1263
Score = 28.7 bits (61), Expect = 1.2
Identities = 22/89 (24%), Positives = 23/89 (25%)
Frame = +1
Query: 643 PPXXXPXXXXPSXXPPXXXRXXXQXXXFXGXPXAXXPXPPPXGTPPXXPAKTXXXPPPXP 822
P P PS PP P P P P P PA + PP
Sbjct: 1126 PSVAAPPVPVPSGAPPVPKPSVAAPPV--PAPSGAPPVPKPSVAAPPVPAPSSGIPPVPK 1183
Query: 823 XPXXXPPXXGGSXXTXFSSXSXXXPPPXP 909
PP S S PP P
Sbjct: 1184 PAAGVPPVPPPSEAPPVPKPSVGVPPVPP 1212
Score = 26.2 bits (55), Expect = 6.5
Identities = 17/53 (32%), Positives = 19/53 (35%)
Frame = +1
Query: 751 PXPPPXGTPPXXPAKTXXXPPPXPXPXXXPPXXGGSXXTXFSSXSXXXPPPXP 909
P P P P P + PP P P PP SS + PPP P
Sbjct: 1023 PVPLPSADAPPIPVPSTA--PPVPIPTSTPPVPKS------SSGAPSAPPPVP 1067
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 28.7 bits (61), Expect = 1.2
Identities = 12/31 (38%), Positives = 12/31 (38%)
Frame = +1
Query: 736 PXAXXPXPPPXGTPPXXPAKTXXXPPPXPXP 828
P P P P P PA PPP P P
Sbjct: 737 PAVIVPTPAPAPIPVPPPAPIMGGPPPPPPP 767
Score = 27.5 bits (58), Expect = 2.8
Identities = 16/55 (29%), Positives = 17/55 (30%)
Frame = +1
Query: 751 PXPPPXGTPPXXPAKTXXXPPPXPXPXXXPPXXGGSXXTXFSSXSXXXPPPXPXA 915
P PPP P PPP P PP + PPP P A
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPP---PPPPGVAGAGPPPPPPPPPA 784
Score = 27.1 bits (57), Expect = 3.7
Identities = 13/31 (41%), Positives = 13/31 (41%)
Frame = +1
Query: 736 PXAXXPXPPPXGTPPXXPAKTXXXPPPXPXP 828
P P PPP PP A PPP P P
Sbjct: 756 PIMGGPPPPP---PPPGVAGAGPPPPPPPPP 783
Score = 25.8 bits (54), Expect = 8.6
Identities = 18/60 (30%), Positives = 20/60 (33%), Gaps = 6/60 (10%)
Frame = +1
Query: 742 AXXPXPPP---XG---TPPXXPAKTXXXPPPXPXPXXXPPXXGGSXXTXFSSXSXXXPPP 903
A P PPP G PP P PPP P P PP ++ P P
Sbjct: 747 APIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP---PPAVSAGGSRYYAPAPQAEPEP 803
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 2.1
Identities = 14/41 (34%), Positives = 15/41 (36%), Gaps = 1/41 (2%)
Frame = +1
Query: 736 PXAXXPXPPPXGTPPXXPAKTXXXPP-PXPXPXXXPPXXGG 855
P + P P PP A T PP P P PP G
Sbjct: 1686 PVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAG 1726
Score = 27.5 bits (58), Expect = 2.8
Identities = 12/33 (36%), Positives = 12/33 (36%)
Frame = +1
Query: 742 AXXPXPPPXGTPPXXPAKTXXXPPPXPXPXXXP 840
A P PPP PP A PP P P
Sbjct: 1704 APTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
Score = 25.8 bits (54), Expect = 8.6
Identities = 10/29 (34%), Positives = 10/29 (34%)
Frame = +1
Query: 736 PXAXXPXPPPXGTPPXXPAKTXXXPPPXP 822
P PPP PP PPP P
Sbjct: 1708 PPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 26.6 bits (56), Expect = 4.9
Identities = 16/41 (39%), Positives = 16/41 (39%), Gaps = 3/41 (7%)
Frame = -2
Query: 842 GGXXXGXGXGGGXXWVFAGXXGGV---PXGGGXGXXAXGXP 729
GG G G GG F G GG P G G G G P
Sbjct: 231 GGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGPGGHGGP 271
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.6 bits (56), Expect = 4.9
Identities = 15/51 (29%), Positives = 17/51 (33%)
Frame = +2
Query: 758 PXPRAPLXPXXQKPXXPPXXXPXPLXXPPXXGXAXXPGFPXXXXXXPPXXP 910
P P++ L P P P P P PP A P PP P
Sbjct: 131 PTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAP----PIPSSLPPPAQP 177
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 25.8 bits (54), Expect = 8.6
Identities = 10/26 (38%), Positives = 10/26 (38%)
Frame = +1
Query: 763 PXGTPPXXPAKTXXXPPPXPXPXXXP 840
P G PP P PP P P P
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPP 30
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,692,867
Number of Sequences: 5004
Number of extensions: 46334
Number of successful extensions: 200
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 88
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 146
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 464508080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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