BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP03_F_N04 (915 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 33 0.043 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 31 0.23 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 29 1.2 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 2.1 SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 27 4.9 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 4.9 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 26 8.6 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 33.5 bits (73), Expect = 0.043 Identities = 23/91 (25%), Positives = 25/91 (27%), Gaps = 2/91 (2%) Frame = +1 Query: 643 PPXXXPXXXXPSXXPPXXXRXXXQXXXFXGXPXAXXPXPP--PXGTPPXXPAKTXXXPPP 816 PP P P+ PP P PP P PP P PP Sbjct: 391 PPPAIPGRSAPAL-PPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPL 449 Query: 817 XPXPXXXPPXXGGSXXTXFSSXSXXXPPPXP 909 P PP G + PPP P Sbjct: 450 PPSAPIAPPLPAGMPAAPPLPPAAPAPPPAP 480 Score = 31.5 bits (68), Expect = 0.17 Identities = 24/86 (27%), Positives = 28/86 (32%), Gaps = 5/86 (5%) Frame = +1 Query: 673 PSXXPPXXXRXXXQXXXFXGXPXAXXPXPPPXGTPPXXPAKTXXXPP-----PXPXPXXX 837 P PP R + G + P PPP P A + PP P P P Sbjct: 311 PPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPP--PRSNAAGSIPLPPQGRSAPPPPPPRS 368 Query: 838 PPXXGGSXXTXFSSXSXXXPPPXPXA 915 P G SS + PP P A Sbjct: 369 APSTGRQPPPLSSSRAVSNPPAPPPA 394 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 31.1 bits (67), Expect = 0.23 Identities = 25/91 (27%), Positives = 26/91 (28%), Gaps = 2/91 (2%) Frame = +1 Query: 643 PPXXXPXXXXPSXXPPXXXRXXXQXXX--FXGXPXAXXPXPPPXGTPPXXPAKTXXXPPP 816 P P PS PP G P A P P P P PA + PP Sbjct: 1027 PSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPS--GAPP 1084 Query: 817 XPXPXXXPPXXGGSXXTXFSSXSXXXPPPXP 909 P P PP S PP P Sbjct: 1085 VPAPSGIPPVPKPSVAAPPVPKPSVAVPPVP 1115 Score = 29.9 bits (64), Expect = 0.53 Identities = 33/131 (25%), Positives = 36/131 (27%), Gaps = 3/131 (2%) Frame = +1 Query: 532 PXXPPPXXXXPXXXXXXPXXXFPXXXGXPLFSR-GFLXPPXXXPXXXXPSXXPPXXXRXX 708 P P P P P G P + PP P P P Sbjct: 1064 PPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPV 1123 Query: 709 XQXXXFXGXPXAXXPXPPPXGTPPXXPAKTXXXPPPXPXPXXXPPXXGGSXXT-XFSSXS 885 + A P P P G PP K PP P P PP S + S Sbjct: 1124 PKPSV------AAPPVPVPSGAPPV--PKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPS 1175 Query: 886 XXXPP-PXPXA 915 PP P P A Sbjct: 1176 SGIPPVPKPAA 1186 Score = 29.9 bits (64), Expect = 0.53 Identities = 30/130 (23%), Positives = 34/130 (26%), Gaps = 2/130 (1%) Frame = +1 Query: 532 PXXPPPXXXXPXXXXXX--PXXXFPXXXGXPLFSRGFLXPPXXXPXXXXPSXXPPXXXRX 705 P P P P P P P+ + PP P P PP Sbjct: 1140 PPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPP 1199 Query: 706 XXQXXXFXGXPXAXXPXPPPXGTPPXXPAKTXXXPPPXPXPXXXPPXXGGSXXTXFSSXS 885 + G P P PPP PP P P P P S S+ Sbjct: 1200 VPKPSV--GVP----PVPPPSTAPPVPTPSAGLPPVPVPTAKAPPVPAPSSEAPSVSTPR 1253 Query: 886 XXXPPPXPXA 915 P P A Sbjct: 1254 SSVPSPHSNA 1263 Score = 28.7 bits (61), Expect = 1.2 Identities = 22/89 (24%), Positives = 23/89 (25%) Frame = +1 Query: 643 PPXXXPXXXXPSXXPPXXXRXXXQXXXFXGXPXAXXPXPPPXGTPPXXPAKTXXXPPPXP 822 P P PS PP P P P P P PA + PP Sbjct: 1126 PSVAAPPVPVPSGAPPVPKPSVAAPPV--PAPSGAPPVPKPSVAAPPVPAPSSGIPPVPK 1183 Query: 823 XPXXXPPXXGGSXXTXFSSXSXXXPPPXP 909 PP S S PP P Sbjct: 1184 PAAGVPPVPPPSEAPPVPKPSVGVPPVPP 1212 Score = 26.2 bits (55), Expect = 6.5 Identities = 17/53 (32%), Positives = 19/53 (35%) Frame = +1 Query: 751 PXPPPXGTPPXXPAKTXXXPPPXPXPXXXPPXXGGSXXTXFSSXSXXXPPPXP 909 P P P P P + PP P P PP SS + PPP P Sbjct: 1023 PVPLPSADAPPIPVPSTA--PPVPIPTSTPPVPKS------SSGAPSAPPPVP 1067 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 28.7 bits (61), Expect = 1.2 Identities = 12/31 (38%), Positives = 12/31 (38%) Frame = +1 Query: 736 PXAXXPXPPPXGTPPXXPAKTXXXPPPXPXP 828 P P P P P PA PPP P P Sbjct: 737 PAVIVPTPAPAPIPVPPPAPIMGGPPPPPPP 767 Score = 27.5 bits (58), Expect = 2.8 Identities = 16/55 (29%), Positives = 17/55 (30%) Frame = +1 Query: 751 PXPPPXGTPPXXPAKTXXXPPPXPXPXXXPPXXGGSXXTXFSSXSXXXPPPXPXA 915 P PPP P PPP P PP + PPP P A Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPP---PPPPGVAGAGPPPPPPPPPA 784 Score = 27.1 bits (57), Expect = 3.7 Identities = 13/31 (41%), Positives = 13/31 (41%) Frame = +1 Query: 736 PXAXXPXPPPXGTPPXXPAKTXXXPPPXPXP 828 P P PPP PP A PPP P P Sbjct: 756 PIMGGPPPPP---PPPGVAGAGPPPPPPPPP 783 Score = 25.8 bits (54), Expect = 8.6 Identities = 18/60 (30%), Positives = 20/60 (33%), Gaps = 6/60 (10%) Frame = +1 Query: 742 AXXPXPPP---XG---TPPXXPAKTXXXPPPXPXPXXXPPXXGGSXXTXFSSXSXXXPPP 903 A P PPP G PP P PPP P P PP ++ P P Sbjct: 747 APIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP---PPAVSAGGSRYYAPAPQAEPEP 803 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 27.9 bits (59), Expect = 2.1 Identities = 14/41 (34%), Positives = 15/41 (36%), Gaps = 1/41 (2%) Frame = +1 Query: 736 PXAXXPXPPPXGTPPXXPAKTXXXPP-PXPXPXXXPPXXGG 855 P + P P PP A T PP P P PP G Sbjct: 1686 PVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAG 1726 Score = 27.5 bits (58), Expect = 2.8 Identities = 12/33 (36%), Positives = 12/33 (36%) Frame = +1 Query: 742 AXXPXPPPXGTPPXXPAKTXXXPPPXPXPXXXP 840 A P PPP PP A PP P P Sbjct: 1704 APTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736 Score = 25.8 bits (54), Expect = 8.6 Identities = 10/29 (34%), Positives = 10/29 (34%) Frame = +1 Query: 736 PXAXXPXPPPXGTPPXXPAKTXXXPPPXP 822 P PPP PP PPP P Sbjct: 1708 PPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736 >SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 273 Score = 26.6 bits (56), Expect = 4.9 Identities = 16/41 (39%), Positives = 16/41 (39%), Gaps = 3/41 (7%) Frame = -2 Query: 842 GGXXXGXGXGGGXXWVFAGXXGGV---PXGGGXGXXAXGXP 729 GG G G GG F G GG P G G G G P Sbjct: 231 GGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGPGGHGGP 271 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.6 bits (56), Expect = 4.9 Identities = 15/51 (29%), Positives = 17/51 (33%) Frame = +2 Query: 758 PXPRAPLXPXXQKPXXPPXXXPXPLXXPPXXGXAXXPGFPXXXXXXPPXXP 910 P P++ L P P P P P PP A P PP P Sbjct: 131 PTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAP----PIPSSLPPPAQP 177 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 25.8 bits (54), Expect = 8.6 Identities = 10/26 (38%), Positives = 10/26 (38%) Frame = +1 Query: 763 PXGTPPXXPAKTXXXPPPXPXPXXXP 840 P G PP P PP P P P Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPP 30 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,692,867 Number of Sequences: 5004 Number of extensions: 46334 Number of successful extensions: 200 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 88 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 146 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 464508080 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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