BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_M21
(937 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 25 0.58
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 25 1.3
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 5.0
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 6.6
SPAC23D3.10c |eng2||endo-1,3-beta-glucanase Eng2|Schizosaccharom... 26 6.6
SPAC8C9.17c |spc34||DASH complex subunit Spc34|Schizosaccharomyc... 26 6.6
SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces... 26 6.6
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 26 8.8
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 25.4 bits (53), Expect(2) = 0.58
Identities = 11/23 (47%), Positives = 12/23 (52%)
Frame = +3
Query: 417 PPPPPXXPPPXXXXGSGGPGXXP 485
PPPPP PPP +GG P
Sbjct: 945 PPPPP--PPPPLVSAAGGKFVSP 965
Score = 23.4 bits (48), Expect(2) = 7.4
Identities = 8/20 (40%), Positives = 9/20 (45%)
Frame = +1
Query: 415 PXXPPXPPPXPXXXXVPGAR 474
P PP PPP P G +
Sbjct: 942 PAFPPPPPPPPPLVSAAGGK 961
Score = 22.6 bits (46), Expect(2) = 0.58
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = +3
Query: 411 PXPPPPPXXP 440
P PPPPP P
Sbjct: 905 PTPPPPPPLP 914
Score = 20.6 bits (41), Expect(2) = 7.4
Identities = 6/14 (42%), Positives = 8/14 (57%)
Frame = +1
Query: 394 VVLTPXPPXXPPXP 435
++ P PP PP P
Sbjct: 901 IITHPTPPPPPPLP 914
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 24.6 bits (51), Expect(2) = 1.3
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = +3
Query: 387 SVCSTYXXPXPPPPP 431
S+ S Y P PPPPP
Sbjct: 182 SLPSDYNPPPPPPPP 196
Score = 22.2 bits (45), Expect(2) = 1.3
Identities = 11/30 (36%), Positives = 12/30 (40%)
Frame = +3
Query: 411 PXPPPPPXXPPPXXXXGSGGPGXXPLXRVN 500
P P PPP PPP S P+ N
Sbjct: 234 PLPAPPPP-PPPTLPPQSTNTSQLPMPSRN 262
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 26.6 bits (56), Expect = 5.0
Identities = 13/31 (41%), Positives = 14/31 (45%), Gaps = 6/31 (19%)
Frame = +3
Query: 411 PXPPP------PPXXPPPXXXXGSGGPGXXP 485
P PPP PP PPP G+G P P
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.2 bits (55), Expect = 6.6
Identities = 12/27 (44%), Positives = 12/27 (44%), Gaps = 2/27 (7%)
Frame = +3
Query: 411 PXPPPPP--XXPPPXXXXGSGGPGXXP 485
P PPPPP PPP GP P
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAP 1731
>SPAC23D3.10c |eng2||endo-1,3-beta-glucanase
Eng2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 706
Score = 26.2 bits (55), Expect = 6.6
Identities = 12/29 (41%), Positives = 17/29 (58%), Gaps = 3/29 (10%)
Frame = -3
Query: 278 HCGTSHGPTWS---TKSWTSGFSQSGSAI 201
+ GT H P+WS + +W +G S G AI
Sbjct: 45 YIGTRHNPSWSHPYSVTWLNGSSYYGLAI 73
>SPAC8C9.17c |spc34||DASH complex subunit Spc34|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 164
Score = 26.2 bits (55), Expect = 6.6
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = -1
Query: 181 NVHLSGIFSLENVAR*RNRQLPQ 113
N+H+SGI +LENV + QL Q
Sbjct: 103 NLHMSGIENLENVLLEQKEQLQQ 125
>SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 713
Score = 26.2 bits (55), Expect = 6.6
Identities = 10/20 (50%), Positives = 11/20 (55%)
Frame = -3
Query: 722 PXPXPPPXTLETIKKERPIG 663
P P PPP L K RP+G
Sbjct: 371 PPPPPPPELLNHSPKSRPLG 390
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 25.8 bits (54), Expect = 8.8
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = +3
Query: 411 PXPPPPPXXPPPXXXXGSGGPG 476
P PPPPP PP PG
Sbjct: 10 PPPPPPPGFEPPSQPPPPPPPG 31
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,917,674
Number of Sequences: 5004
Number of extensions: 52453
Number of successful extensions: 217
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 122
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 189
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 475330268
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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