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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP03_F_M21
         (937 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1...    25   0.58 
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ...    25   1.3  
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||...    27   5.0  
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    26   6.6  
SPAC23D3.10c |eng2||endo-1,3-beta-glucanase Eng2|Schizosaccharom...    26   6.6  
SPAC8C9.17c |spc34||DASH complex subunit Spc34|Schizosaccharomyc...    26   6.6  
SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces...    26   6.6  
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac...    26   8.8  

>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1841

 Score = 25.4 bits (53), Expect(2) = 0.58
 Identities = 11/23 (47%), Positives = 12/23 (52%)
 Frame = +3

Query: 417  PPPPPXXPPPXXXXGSGGPGXXP 485
            PPPPP  PPP     +GG    P
Sbjct: 945  PPPPP--PPPPLVSAAGGKFVSP 965



 Score = 23.4 bits (48), Expect(2) = 7.4
 Identities = 8/20 (40%), Positives = 9/20 (45%)
 Frame = +1

Query: 415  PXXPPXPPPXPXXXXVPGAR 474
            P  PP PPP P      G +
Sbjct: 942  PAFPPPPPPPPPLVSAAGGK 961



 Score = 22.6 bits (46), Expect(2) = 0.58
 Identities = 7/10 (70%), Positives = 7/10 (70%)
 Frame = +3

Query: 411 PXPPPPPXXP 440
           P PPPPP  P
Sbjct: 905 PTPPPPPPLP 914



 Score = 20.6 bits (41), Expect(2) = 7.4
 Identities = 6/14 (42%), Positives = 8/14 (57%)
 Frame = +1

Query: 394 VVLTPXPPXXPPXP 435
           ++  P PP  PP P
Sbjct: 901 IITHPTPPPPPPLP 914


>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1420

 Score = 24.6 bits (51), Expect(2) = 1.3
 Identities = 9/15 (60%), Positives = 10/15 (66%)
 Frame = +3

Query: 387 SVCSTYXXPXPPPPP 431
           S+ S Y  P PPPPP
Sbjct: 182 SLPSDYNPPPPPPPP 196



 Score = 22.2 bits (45), Expect(2) = 1.3
 Identities = 11/30 (36%), Positives = 12/30 (40%)
 Frame = +3

Query: 411 PXPPPPPXXPPPXXXXGSGGPGXXPLXRVN 500
           P P PPP  PPP     S      P+   N
Sbjct: 234 PLPAPPPP-PPPTLPPQSTNTSQLPMPSRN 262


>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1461

 Score = 26.6 bits (56), Expect = 5.0
 Identities = 13/31 (41%), Positives = 14/31 (45%), Gaps = 6/31 (19%)
 Frame = +3

Query: 411 PXPPP------PPXXPPPXXXXGSGGPGXXP 485
           P PPP      PP  PPP    G+G P   P
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 26.2 bits (55), Expect = 6.6
 Identities = 12/27 (44%), Positives = 12/27 (44%), Gaps = 2/27 (7%)
 Frame = +3

Query: 411  PXPPPPP--XXPPPXXXXGSGGPGXXP 485
            P PPPPP    PPP       GP   P
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAP 1731


>SPAC23D3.10c |eng2||endo-1,3-beta-glucanase
           Eng2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 706

 Score = 26.2 bits (55), Expect = 6.6
 Identities = 12/29 (41%), Positives = 17/29 (58%), Gaps = 3/29 (10%)
 Frame = -3

Query: 278 HCGTSHGPTWS---TKSWTSGFSQSGSAI 201
           + GT H P+WS   + +W +G S  G AI
Sbjct: 45  YIGTRHNPSWSHPYSVTWLNGSSYYGLAI 73


>SPAC8C9.17c |spc34||DASH complex subunit Spc34|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 164

 Score = 26.2 bits (55), Expect = 6.6
 Identities = 12/23 (52%), Positives = 16/23 (69%)
 Frame = -1

Query: 181 NVHLSGIFSLENVAR*RNRQLPQ 113
           N+H+SGI +LENV   +  QL Q
Sbjct: 103 NLHMSGIENLENVLLEQKEQLQQ 125


>SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 713

 Score = 26.2 bits (55), Expect = 6.6
 Identities = 10/20 (50%), Positives = 11/20 (55%)
 Frame = -3

Query: 722 PXPXPPPXTLETIKKERPIG 663
           P P PPP  L    K RP+G
Sbjct: 371 PPPPPPPELLNHSPKSRPLG 390


>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
           Spp42|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2363

 Score = 25.8 bits (54), Expect = 8.8
 Identities = 10/22 (45%), Positives = 10/22 (45%)
 Frame = +3

Query: 411 PXPPPPPXXPPPXXXXGSGGPG 476
           P PPPPP   PP        PG
Sbjct: 10  PPPPPPPGFEPPSQPPPPPPPG 31


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,917,674
Number of Sequences: 5004
Number of extensions: 52453
Number of successful extensions: 217
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 122
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 189
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 475330268
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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