Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_M02
(844 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 30 0.47
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 29 0.82
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 28 1.4
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 1.9
SPBC1685.01 |pmp1||dual-specificity MAP kinase phosphatase Pmp1|... 27 2.5
SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein ... 27 3.3
SPCC622.15c |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 27 4.4
SPAC4D7.10c |||SAGA complex subunit Spt20 |Schizosaccharomyces p... 27 4.4
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 26 7.7
SPCC1753.03c |rec7||meiotic recombination protein Rec7 |Schizosa... 26 7.7
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 26 7.7
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 26 7.7
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 29.9 bits (64), Expect = 0.47
Identities = 14/34 (41%), Positives = 14/34 (41%)
Frame = +2
Query: 653 PPPPPPXXXXXPXXPXSXPXXXPXXPXXPPPXXP 754
P PPPP P P S P P PPP P
Sbjct: 1705 PTPPPPPMSVPP--PPSAPPMPAGPPSAPPPPLP 1736
Score = 26.6 bits (56), Expect = 4.4
Identities = 18/58 (31%), Positives = 18/58 (31%), Gaps = 3/58 (5%)
Frame = +3
Query: 657 PPPXPXXXXXXXXXFPXXXXXPXXPXXPPXXXPXPXXP---XPPPLXXLSFFSXPXXG 821
PP P P P PP P P P PPPL S S P G
Sbjct: 1690 PPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNPG 1747
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 29.1 bits (62), Expect = 0.82
Identities = 18/49 (36%), Positives = 18/49 (36%)
Frame = -2
Query: 753 GXXGGGXXGXXGXXXGXEXGXXGXXXXXGGGGGGXPXXXFFXXGGXXXP 607
G GGG G G G G G GG GGG P GG P
Sbjct: 224 GGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGG-PGGFGGGPGGHGGP 271
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 28.3 bits (60), Expect = 1.4
Identities = 12/34 (35%), Positives = 12/34 (35%)
Frame = +2
Query: 653 PPPPPPXXXXXPXXPXSXPXXXPXXPXXPPPXXP 754
P PPP P P P P PPP P
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 27.9 bits (59), Expect = 1.9
Identities = 12/34 (35%), Positives = 12/34 (35%)
Frame = +2
Query: 653 PPPPPPXXXXXPXXPXSXPXXXPXXPXXPPPXXP 754
PPPPPP P P P PP P
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPP 765
Score = 26.6 bits (56), Expect = 4.4
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = +2
Query: 605 GGXXXPPXXKKXXXGXPPPPPP 670
GG PP PPPPPP
Sbjct: 759 GGPPPPPPPPGVAGAGPPPPPP 780
Score = 25.8 bits (54), Expect = 7.7
Identities = 13/33 (39%), Positives = 13/33 (39%)
Frame = +2
Query: 647 GXPPPPPPXXXXXPXXPXSXPXXXPXXPXXPPP 745
G PPPPPP P P P PPP
Sbjct: 759 GGPPPPPP--------PPGVAGAGPPPPPPPPP 783
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 27.9 bits (59), Expect = 1.9
Identities = 13/34 (38%), Positives = 13/34 (38%), Gaps = 1/34 (2%)
Frame = +2
Query: 656 PPPPPXXXXXPXXPXSXPXXXPXXPXXP-PPXXP 754
PP PP P P P P P P PP P
Sbjct: 447 PPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAP 480
Score = 27.1 bits (57), Expect = 3.3
Identities = 16/64 (25%), Positives = 16/64 (25%)
Frame = +3
Query: 621 PPXXKXXXXGXXPPPXPXXXXXXXXXFPXXXXXPXXPXXPPXXXPXPXXPXPPPLXXLSF 800
PP PPP P P P PP PPPL
Sbjct: 325 PPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRA 384
Query: 801 FSXP 812
S P
Sbjct: 385 VSNP 388
Score = 26.6 bits (56), Expect = 4.4
Identities = 11/31 (35%), Positives = 11/31 (35%)
Frame = +2
Query: 653 PPPPPPXXXXXPXXPXSXPXXXPXXPXXPPP 745
PPPPPP P P PPP
Sbjct: 312 PPPPPPSRRNRGKPPIGNGSSNSSLPPPPPP 342
Score = 25.8 bits (54), Expect = 7.7
Identities = 14/47 (29%), Positives = 15/47 (31%), Gaps = 2/47 (4%)
Frame = +2
Query: 620 PPXXKKXXXGXPPPPP--PXXXXXPXXPXSXPXXXPXXPXXPPPXXP 754
PP PP PP P P + P P P PP P
Sbjct: 436 PPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAP 482
>SPBC1685.01 |pmp1||dual-specificity MAP kinase phosphatase
Pmp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 278
Score = 27.5 bits (58), Expect = 2.5
Identities = 17/56 (30%), Positives = 25/56 (44%)
Frame = +2
Query: 161 VVENADSGNGYEPIDNRPYIVNPPKDYNPNGNGYEPIDNGAYYVDPPQGRPYFKPT 328
++EN + + + P + PK PN N +P NG + PP Y KPT
Sbjct: 23 MLENEEEASHSQLFTPCPVPPSFPKASKPNSN--QPYPNGPVCIYPPNIYLYAKPT 76
>SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein
Lsb1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 296
Score = 27.1 bits (57), Expect = 3.3
Identities = 12/34 (35%), Positives = 12/34 (35%)
Frame = +2
Query: 653 PPPPPPXXXXXPXXPXSXPXXXPXXPXXPPPXXP 754
PPPPPP P S P PP P
Sbjct: 206 PPPPPPQQNYPPAASSSAPPMQYQQTAYPPQQAP 239
>SPCC622.15c |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 557
Score = 26.6 bits (56), Expect = 4.4
Identities = 14/32 (43%), Positives = 17/32 (53%), Gaps = 4/32 (12%)
Frame = +2
Query: 236 DYNPNG-NGYEPIDNGAYYVDPPQG---RPYF 319
DYN N N Y PI N Y+++ G PYF
Sbjct: 119 DYNNNRKNFYPPIQNSTYFINATGGIDSMPYF 150
>SPAC4D7.10c |||SAGA complex subunit Spt20 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 473
Score = 26.6 bits (56), Expect = 4.4
Identities = 13/59 (22%), Positives = 25/59 (42%)
Frame = +2
Query: 155 VKVVENADSGNGYEPIDNRPYIVNPPKDYNPNGNGYEPIDNGAYYVDPPQGRPYFKPTP 331
V+++++ S + + + +P N + N N +P D PP +PTP
Sbjct: 126 VRIIDHRQSPSADQTVQPQPGSTNQQQQNNTNPINNQPEDTKPNTNSPPVYHTVLRPTP 184
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 25.8 bits (54), Expect = 7.7
Identities = 12/34 (35%), Positives = 12/34 (35%)
Frame = -2
Query: 753 GXXGGGXXGXXGXXXGXEXGXXGXXXXXGGGGGG 652
G GG G G G G G GG GG
Sbjct: 30 GGRGGARGGGRGGARGGRGGRGGARGGRGGSSGG 63
>SPCC1753.03c |rec7||meiotic recombination protein Rec7
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 339
Score = 25.8 bits (54), Expect = 7.7
Identities = 11/37 (29%), Positives = 18/37 (48%)
Frame = +3
Query: 240 TTLMETATNLSTTVHITWTLPKADLTSSLPPFPGARG 350
TT + +TN + +P A++ S+ PP RG
Sbjct: 174 TTAVNNSTNYVNPAPLQHVMPNAEIFSNTPPLKRFRG 210
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 25.8 bits (54), Expect = 7.7
Identities = 17/56 (30%), Positives = 24/56 (42%)
Frame = +3
Query: 171 TLTQETATNLLTTARTSLILPKITTLMETATNLSTTVHITWTLPKADLTSSLPPFP 338
T T T TN TS T++ T + S + IT T+P +S+ P P
Sbjct: 153 TSTSSTDTNSNPLPTTSTSCTTSTSIPPTGGSSSLSTPITPTVPPTSTSSTSIPIP 208
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 25.8 bits (54), Expect = 7.7
Identities = 12/36 (33%), Positives = 12/36 (33%)
Frame = +2
Query: 647 GXPPPPPPXXXXXPXXPXSXPXXXPXXPXXPPPXXP 754
G PP P P P P S P PP P
Sbjct: 1177 GIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPP 1212
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,403,642
Number of Sequences: 5004
Number of extensions: 44476
Number of successful extensions: 233
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 114
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 416455520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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