Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_L23
(873 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g00234.1 68417.m00028 expressed protein 30 1.8
At4g00290.1 68417.m00036 mechanosensitive ion channel domain-con... 29 3.1
At3g14140.1 68416.m01788 oxidoreductase, 2OG-Fe(II) oxygenase fa... 29 5.4
At1g80950.1 68414.m09498 phospholipid/glycerol acyltransferase f... 29 5.4
At1g04620.1 68414.m00456 coenzyme F420 hydrogenase family / dehy... 29 5.4
At4g02500.1 68417.m00341 galactosyl transferase GMA12/MNN10 fami... 28 9.4
At1g55350.4 68414.m06326 calpain-type cysteine protease family i... 28 9.4
At1g55350.3 68414.m06325 calpain-type cysteine protease family i... 28 9.4
At1g55350.2 68414.m06324 calpain-type cysteine protease family i... 28 9.4
At1g55350.1 68414.m06323 calpain-type cysteine protease family i... 28 9.4
>At4g00234.1 68417.m00028 expressed protein
Length = 263
Score = 30.3 bits (65), Expect = 1.8
Identities = 20/83 (24%), Positives = 37/83 (44%)
Frame = -1
Query: 441 MSPTICKSAFVFSSTVLALVSIWKSDICWFFRPVMFKFLKRITTPASFGAITSNDVTTSG 262
++PT + + FS TV V + + WF + R+ + SFG + + V T
Sbjct: 177 VAPTTIAAQY-FSPTVKGAVIL---SVVWFLYRWKTNVITRMLSAKSFGGLDRDKVLTLD 232
Query: 261 SIGHMSYCGIPLLVFSKNCSVAL 193
+ + I L+ ++ C VA+
Sbjct: 233 KVSSVGLFAIGLMASAEACGVAV 255
>At4g00290.1 68417.m00036 mechanosensitive ion channel
domain-containing protein / MS ion channel
domain-containing protein weak similarity to SP|P77338
Potassium efflux system kefA {Escherichia coli};
contains Pfam profile PF00924: Mechanosensitive ion
channel
Length = 497
Score = 29.5 bits (63), Expect = 3.1
Identities = 20/83 (24%), Positives = 36/83 (43%)
Frame = -1
Query: 441 MSPTICKSAFVFSSTVLALVSIWKSDICWFFRPVMFKFLKRITTPASFGAITSNDVTTSG 262
++PT + + FS TV V + + WF + R+ + SFG + V T
Sbjct: 229 VAPTTIAAQY-FSPTVKGAVIL---SLVWFLYRWKTNVITRMLSAKSFGGLDREKVLTLD 284
Query: 261 SIGHMSYCGIPLLVFSKNCSVAL 193
+ + I L+ ++ C VA+
Sbjct: 285 KVSSVGLFAIGLMASAEACGVAV 307
>At3g14140.1 68416.m01788 oxidoreductase, 2OG-Fe(II) oxygenase
family protein low similarity to alkB protein
[Escherichia coli][SP|P05050], alkB [Caulobacter
crescentus][GI:2055386]; contains Pfam domain PF03171
2OG-Fe(II) oxygenase superfamily
Length = 452
Score = 28.7 bits (61), Expect = 5.4
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = +3
Query: 747 NVVHNIRASAKILPASSFFRK 809
NV H +R+ KILP FFRK
Sbjct: 425 NVFHGVRSIRKILPPRLFFRK 445
>At1g80950.1 68414.m09498 phospholipid/glycerol acyltransferase
family protein low similarity to SP|Q59601
1-acyl-sn-glycerol-3-phosphate acyltransferase (EC
2.3.1.51) {Neisseria gonorrhoeae}; contains Pfam profile
PF01553: Acyltransferase
Length = 398
Score = 28.7 bits (61), Expect = 5.4
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = -1
Query: 741 SRILLFYK*LLVLTFSKAPYRGPKE 667
S +LL+Y V T APYRGP+E
Sbjct: 83 SILLLYYLICRVFTLFSAPYRGPEE 107
>At1g04620.1 68414.m00456 coenzyme F420 hydrogenase family /
dehydrogenase, beta subunit family contains Pfam
PF04432: Coenzyme F420 hydrogenase/dehydrogenase, beta
domain; similar to Coenzyme F420 hydrogenase beta
subunit (SP:Q00391) {Methanobacterium
thermoautotrophicum}
Length = 462
Score = 28.7 bits (61), Expect = 5.4
Identities = 14/30 (46%), Positives = 17/30 (56%)
Frame = +1
Query: 277 VIGCNCSKRCGSRDTLQKFKHHGSKEPANI 366
V+G NC G+RD L KF SKEP +
Sbjct: 230 VLGTNCVDN-GTRDGLDKFLKAASKEPETV 258
>At4g02500.1 68417.m00341 galactosyl transferase GMA12/MNN10 family
protein low similarity to
alpha-1,2-galactosyltransferase, Schizosaccharomyces
pombe [SP|Q09174]
Length = 461
Score = 27.9 bits (59), Expect = 9.4
Identities = 12/22 (54%), Positives = 13/22 (59%)
Frame = +2
Query: 77 TSKWLL*KYSWFLCLLDTWLPM 142
T +LL W L LLDTW PM
Sbjct: 270 TGSFLLRNNQWALDLLDTWAPM 291
>At1g55350.4 68414.m06326 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 27.9 bits (59), Expect = 9.4
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = -3
Query: 262 IDRPYVVLWYPFTGFLQKLLCRTAKALHVXQVCRV 158
ID +V +W F G+L LL TAKA V R+
Sbjct: 1223 IDTEWVYMWDKFGGYLLLLLGLTAKAERVQDEVRL 1257
>At1g55350.3 68414.m06325 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 27.9 bits (59), Expect = 9.4
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = -3
Query: 262 IDRPYVVLWYPFTGFLQKLLCRTAKALHVXQVCRV 158
ID +V +W F G+L LL TAKA V R+
Sbjct: 1223 IDTEWVYMWDKFGGYLLLLLGLTAKAERVQDEVRL 1257
>At1g55350.2 68414.m06324 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 27.9 bits (59), Expect = 9.4
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = -3
Query: 262 IDRPYVVLWYPFTGFLQKLLCRTAKALHVXQVCRV 158
ID +V +W F G+L LL TAKA V R+
Sbjct: 1223 IDTEWVYMWDKFGGYLLLLLGLTAKAERVQDEVRL 1257
>At1g55350.1 68414.m06323 calpain-type cysteine protease family
identical to calpain-like protein GI:20268660 from
[Arabidopsis thaliana]; contains Pfam profiles: PF00648
Calpain family cysteine protease, PF01067 Calpain large
subunit,domain III; identical to cDNA calpain-like
protein GI:20268659
Length = 2151
Score = 27.9 bits (59), Expect = 9.4
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = -3
Query: 262 IDRPYVVLWYPFTGFLQKLLCRTAKALHVXQVCRV 158
ID +V +W F G+L LL TAKA V R+
Sbjct: 1223 IDTEWVYMWDKFGGYLLLLLGLTAKAERVQDEVRL 1257
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,127,952
Number of Sequences: 28952
Number of extensions: 382085
Number of successful extensions: 925
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 896
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 924
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2048424000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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