BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP03_F_L14 (880 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC613.05c |rpl35||60S ribosomal protein L35|Schizosaccharomyce... 105 6e-24 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 31 0.057 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 30 0.50 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 0.66 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 2.7 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 2.7 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 27 4.7 SPAC3H8.06 |aur1||inositol phosphorylceramide synthase |Schizosa... 27 4.7 SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 26 6.1 >SPCC613.05c |rpl35||60S ribosomal protein L35|Schizosaccharomyces pombe|chr 3|||Manual Length = 122 Score = 105 bits (253), Expect = 6e-24 Identities = 56/120 (46%), Positives = 73/120 (60%) Frame = +1 Query: 121 VKCSELRTKDXXXXXXXXXXXXXXXTNLRVAKVTGGVASKLSKIRVVRKAIARVYIVYHQ 300 +K ELR + +LRV K+ GG SKLSKI+ RK IAR+ V ++ Sbjct: 3 LKTFELRKQSQENLAEQLQELRQELASLRVQKIAGGSGSKLSKIKTTRKDIARILTVINE 62 Query: 301 KMKVNLRNHYKNKKYKPLDLRAKKTRAMRKALTKHEAKIKTRKEIRKKSLFPPRVYAVKA 480 ++ R YKNKKY PLDLR KKTRA+R+ALT +E KT K+I+K+ FP R YA+KA Sbjct: 63 SNRLAAREAYKNKKYIPLDLRQKKTRAIRRALTPYEQSRKTLKQIKKERYFPLRKYALKA 122 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 31.1 bits (67), Expect(2) = 0.057 Identities = 20/58 (34%), Positives = 23/58 (39%), Gaps = 2/58 (3%) Frame = +2 Query: 683 PPPPXKXXRXQXTXFSXGPXXGAGXPXXXPPPXQPPPXPXX--SQPXPGXXLFSPPPP 850 PPPP + R + P G G PP PPP S P P +PPPP Sbjct: 314 PPPPSRRNRGK-------PPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPP 364 Score = 27.1 bits (57), Expect = 3.5 Identities = 21/78 (26%), Positives = 21/78 (26%) Frame = +2 Query: 614 PPXGGGXXXPPPSXNTXLGXXXXPPPPXKXXRXQXTXFSXGPXXGAGXPXXXPPPXQPPP 793 PP PP N PPPP R P G P PPP P Sbjct: 315 PPPSRRNRGKPPIGNGSSNSSLPPPPP--PPRSNAAGSIPLPPQGRSAP-PPPPPRSAPS 371 Query: 794 XPXXSQPXPGXXLFSPPP 847 P S PP Sbjct: 372 TGRQPPPLSSSRAVSNPP 389 Score = 27.1 bits (57), Expect = 3.5 Identities = 18/72 (25%), Positives = 22/72 (30%), Gaps = 2/72 (2%) Frame = +2 Query: 641 PPPSXNTXLGXXXXPPPPXKXXRXQXTXFSXGPXXGAGXPXXXPPPXQPPPXPXXSQP-- 814 PPP ++ PPP R G P PP PP P P Sbjct: 376 PPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSA 435 Query: 815 XPGXXLFSPPPP 850 P + +P P Sbjct: 436 PPSLPMGAPAAP 447 Score = 27.1 bits (57), Expect = 3.5 Identities = 10/23 (43%), Positives = 10/23 (43%) Frame = +1 Query: 517 PXPPXTPXXPPPXXPPXPXPXXP 585 P PP P PP PP P P Sbjct: 418 PTPPSLPPSAPPSLPPSAPPSLP 440 Score = 20.6 bits (41), Expect(2) = 0.057 Identities = 8/18 (44%), Positives = 8/18 (44%) Frame = +2 Query: 641 PPPSXNTXLGXXXXPPPP 694 PPPS T PP P Sbjct: 257 PPPSNGTVSSPPNSPPRP 274 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 29.9 bits (64), Expect = 0.50 Identities = 14/30 (46%), Positives = 15/30 (50%) Frame = +3 Query: 762 LXSRPPXNXPPXPXXPNPXXGXXFFPPPPP 851 + S PP N PP P P P PPPPP Sbjct: 1 MASLPPGNPPPPP--PPPGFEPPSQPPPPP 28 Score = 28.7 bits (61), Expect = 1.2 Identities = 16/35 (45%), Positives = 16/35 (45%), Gaps = 1/35 (2%) Frame = +2 Query: 749 AGXPXXXPPPXQPPP-XPXXSQPXPGXXLFSPPPP 850 A P PPP PPP SQP P PPPP Sbjct: 2 ASLPPGNPPPPPPPPGFEPPSQPPP------PPPP 30 Score = 28.3 bits (60), Expect = 1.5 Identities = 10/20 (50%), Positives = 10/20 (50%) Frame = +1 Query: 517 PXPPXTPXXPPPXXPPXPXP 576 P PP P PP PP P P Sbjct: 10 PPPPPPPGFEPPSQPPPPPP 29 Score = 27.5 bits (58), Expect = 2.7 Identities = 14/31 (45%), Positives = 14/31 (45%) Frame = +2 Query: 773 PPXQPPPXPXXSQPXPGXXLFSPPPPXGXXG 865 PP PPP P P PG S PPP G Sbjct: 5 PPGNPPPPP----PPPGFEPPSQPPPPPPPG 31 Score = 26.2 bits (55), Expect = 6.1 Identities = 10/27 (37%), Positives = 12/27 (44%) Frame = +3 Query: 495 TIPKXNXXXXPXNPPXPPPXXPPXPXP 575 ++P N P P PP PP P P Sbjct: 3 SLPPGNPPPPPPPPGFEPPSQPPPPPP 29 Score = 25.8 bits (54), Expect = 8.1 Identities = 12/29 (41%), Positives = 12/29 (41%) Frame = +2 Query: 770 PPPXQPPPXPXXSQPXPGXXLFSPPPPXG 856 PP PPP P P PPPP G Sbjct: 5 PPGNPPPPPPPPGFEPPSQP--PPPPPPG 31 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 29.5 bits (63), Expect = 0.66 Identities = 14/40 (35%), Positives = 15/40 (37%) Frame = +3 Query: 759 PLXSRPPXNXPPXPXXPNPXXGXXFFPPPPPXXXXAXXXP 878 P PP + PP P P G PPPP A P Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVP 1744 Score = 26.6 bits (56), Expect = 4.7 Identities = 10/25 (40%), Positives = 10/25 (40%) Frame = +1 Query: 496 PSXXXTXPXPPXTPXXPPPXXPPXP 570 P P PP PPP PP P Sbjct: 1700 PQMSAPTPPPPPMSVPPPPSAPPMP 1724 Score = 25.8 bits (54), Expect = 8.1 Identities = 16/63 (25%), Positives = 17/63 (26%) Frame = +2 Query: 626 GGXXXPPPSXNTXLGXXXXPPPPXKXXRXQXTXFSXGPXXGAGXPXXXPPPXQPPPXPXX 805 GG P + PP S P A PP PPP P Sbjct: 1679 GGMAPAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPAS 1738 Query: 806 SQP 814 S P Sbjct: 1739 SAP 1741 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 27.5 bits (58), Expect = 2.7 Identities = 15/38 (39%), Positives = 16/38 (42%) Frame = +2 Query: 737 PXXGAGXPXXXPPPXQPPPXPXXSQPXPGXXLFSPPPP 850 P AG P PPP + PP P S P S PP Sbjct: 1182 PKPAAGVPPV-PPPSEAPPVPKPSVGVPPVPPPSTAPP 1218 Score = 26.2 bits (55), Expect = 6.1 Identities = 14/49 (28%), Positives = 17/49 (34%), Gaps = 1/49 (2%) Frame = +2 Query: 737 PXXGAGXPXXXPPPXQPP-PXPXXSQPXPGXXLFSPPPPXGXXGXXVXP 880 P P P PP P P + P P + +PP P G P Sbjct: 1134 PVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVP 1182 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 27.5 bits (58), Expect = 2.7 Identities = 17/56 (30%), Positives = 19/56 (33%) Frame = +2 Query: 683 PPPPXKXXRXQXTXFSXGPXXGAGXPXXXPPPXQPPPXPXXSQPXPGXXLFSPPPP 850 PPPP + P PPP PPP + P P PPPP Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPP----PPPPP 783 Score = 25.8 bits (54), Expect = 8.1 Identities = 11/27 (40%), Positives = 11/27 (40%) Frame = +2 Query: 614 PPXGGGXXXPPPSXNTXLGXXXXPPPP 694 P GG PPP G PPPP Sbjct: 756 PIMGGPPPPPPPPGVAGAGPPPPPPPP 782 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 26.6 bits (56), Expect = 4.7 Identities = 10/22 (45%), Positives = 11/22 (50%) Frame = +2 Query: 785 PPPXPXXSQPXPGXXLFSPPPP 850 PPP P S + SPPPP Sbjct: 908 PPPPPTASMTASAPAIASPPPP 929 >SPAC3H8.06 |aur1||inositol phosphorylceramide synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 422 Score = 26.6 bits (56), Expect = 4.7 Identities = 11/32 (34%), Positives = 18/32 (56%), Gaps = 3/32 (9%) Frame = -2 Query: 492 IIYSSFNGIDSRW---EERFLSDLFPRLDLCF 406 +++ +F + + W E FLS +FPR CF Sbjct: 246 VVFGAFPSLHAGWAMLEALFLSHVFPRYRFCF 277 >SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 1420 Score = 26.2 bits (55), Expect = 6.1 Identities = 11/30 (36%), Positives = 13/30 (43%) Frame = +3 Query: 762 LXSRPPXNXPPXPXXPNPXXGXXFFPPPPP 851 + S PP PP P + PPPPP Sbjct: 164 MVSPPPSFQPPSAAAPATSLPSDYNPPPPP 193 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,635,464 Number of Sequences: 5004 Number of extensions: 47054 Number of successful extensions: 310 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 131 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 256 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 440481800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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