BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP03_F_L08 (1044 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 37 0.004 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 36 0.007 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.36 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 1.1 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 29 1.4 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 29 1.4 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 26 7.6 SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 26 7.6 SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 22 8.4 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 37.1 bits (82), Expect = 0.004 Identities = 18/38 (47%), Positives = 19/38 (50%) Frame = +3 Query: 723 PPPPPXPXSXAXLPTPPPPXXRXSXLFXPPPPGPXGXP 836 PPPPP P S A P PP R + PPPP P P Sbjct: 337 PPPPPPPRSNAAGSIPLPPQGRSA----PPPPPPRSAP 370 Score = 31.1 bits (67), Expect = 0.27 Identities = 13/33 (39%), Positives = 14/33 (42%) Frame = +3 Query: 726 PPPPXPXSXAXLPTPPPPXXRXSXLFXPPPPGP 824 PPPP P S PPP + PP P P Sbjct: 361 PPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPP 393 Score = 29.9 bits (64), Expect = 0.62 Identities = 13/33 (39%), Positives = 14/33 (42%) Frame = +3 Query: 726 PPPPXPXSXAXLPTPPPPXXRXSXLFXPPPPGP 824 PPPP P S PP + PPPP P Sbjct: 311 PPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPP 343 Score = 29.1 bits (62), Expect = 1.1 Identities = 17/49 (34%), Positives = 18/49 (36%), Gaps = 8/49 (16%) Frame = +3 Query: 693 GGGSXLLXXXPPPP--------PXPXSXAXLPTPPPPXXRXSXLFXPPP 815 G + L PPPP P P P PPPP S PPP Sbjct: 330 GSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPP 378 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 36.3 bits (80), Expect = 0.007 Identities = 15/34 (44%), Positives = 16/34 (47%) Frame = +3 Query: 723 PPPPPXPXSXAXLPTPPPPXXRXSXLFXPPPPGP 824 P PPP P P PPPP + PPPP P Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783 Score = 34.7 bits (76), Expect = 0.022 Identities = 18/45 (40%), Positives = 20/45 (44%), Gaps = 4/45 (8%) Frame = +3 Query: 708 LLXXXPPPPP----XPXSXAXLPTPPPPXXRXSXLFXPPPPGPXG 830 LL PPPPP + A +P PPP PPPPG G Sbjct: 728 LLKSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAG 772 Score = 31.1 bits (67), Expect = 0.27 Identities = 11/24 (45%), Positives = 13/24 (54%) Frame = +3 Query: 708 LLXXXPPPPPXPXSXAXLPTPPPP 779 ++ PPPPP P P PPPP Sbjct: 757 IMGGPPPPPPPPGVAGAGPPPPPP 780 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 30.7 bits (66), Expect = 0.36 Identities = 14/33 (42%), Positives = 15/33 (45%) Frame = +3 Query: 723 PPPPPXPXSXAXLPTPPPPXXRXSXLFXPPPPG 821 PPPP P A P+ PPP S P PG Sbjct: 1715 PPPPSAPPMPAGPPSAPPPPLPASSAPSVPNPG 1747 Score = 28.7 bits (61), Expect = 1.4 Identities = 16/35 (45%), Positives = 17/35 (48%), Gaps = 1/35 (2%) Frame = +3 Query: 723 PPPPPXPXSXAXLPTPPP-PXXRXSXLFXPPPPGP 824 P PPP P S P+ PP P S PPPP P Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPS---APPPPLP 1736 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 29.1 bits (62), Expect = 1.1 Identities = 14/35 (40%), Positives = 16/35 (45%) Frame = +3 Query: 732 PPXPXSXAXLPTPPPPXXRXSXLFXPPPPGPXGXP 836 PP P S + P+ PPP S P P P G P Sbjct: 1050 PPVPKSSSGAPSAPPPVPAPSSEI-PSIPAPSGAP 1083 Score = 28.3 bits (60), Expect = 1.9 Identities = 15/40 (37%), Positives = 18/40 (45%), Gaps = 3/40 (7%) Frame = +3 Query: 726 PPPPXPXSXAXLPTPP---PPXXRXSXLFXPPPPGPXGXP 836 PP P P +P P PP + S + PP P P G P Sbjct: 1083 PPVPAPSGIPPVPKPSVAAPPVPKPS-VAVPPVPAPSGAP 1121 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 28.7 bits (61), Expect = 1.4 Identities = 15/33 (45%), Positives = 16/33 (48%) Frame = +3 Query: 723 PPPPPXPXSXAXLPTPPPPXXRXSXLFXPPPPG 821 PPPPP P P+ PPP PPPPG Sbjct: 9 PPPPPPPPGFEP-PSQPPP---------PPPPG 31 Score = 28.3 bits (60), Expect = 1.9 Identities = 11/23 (47%), Positives = 11/23 (47%) Frame = +3 Query: 762 PTPPPPXXRXSXLFXPPPPGPXG 830 P PPPP PPPP P G Sbjct: 9 PPPPPPPPGFEPPSQPPPPPPPG 31 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 28.7 bits (61), Expect = 1.4 Identities = 10/17 (58%), Positives = 10/17 (58%) Frame = +3 Query: 723 PPPPPXPXSXAXLPTPP 773 PPPPP P A P PP Sbjct: 6 PPPPPAPAPAAAAPAPP 22 Score = 27.1 bits (57), Expect = 4.4 Identities = 16/37 (43%), Positives = 16/37 (43%) Frame = +3 Query: 726 PPPPXPXSXAXLPTPPPPXXRXSXLFXPPPPGPXGXP 836 P PP P S PT PP R S PPP P P Sbjct: 128 PAPPTPQSELRPPTSAPP--RPSI----PPPSPASAP 158 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 26.2 bits (55), Expect = 7.6 Identities = 13/33 (39%), Positives = 16/33 (48%), Gaps = 3/33 (9%) Frame = +3 Query: 723 PPPPPXPXSXAXLP---TPPPPXXRXSXLFXPP 812 PPPPP A P +PPPP + + PP Sbjct: 908 PPPPPTASMTASAPAIASPPPP--KVGETYHPP 938 >SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Schizosaccharomyces pombe|chr 1|||Manual Length = 1995 Score = 26.2 bits (55), Expect = 7.6 Identities = 16/42 (38%), Positives = 18/42 (42%) Frame = +3 Query: 699 GSXLLXXXPPPPPXPXSXAXLPTPPPPXXRXSXLFXPPPPGP 824 GS L PPPP P + LP PP + PP GP Sbjct: 1873 GSDLSNQAAPPPPPPMA---LPKAGPPSAAPTSAL--PPAGP 1909 >SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1|||Manual Length = 1841 Score = 22.2 bits (45), Expect(2) = 8.4 Identities = 7/10 (70%), Positives = 7/10 (70%) Frame = +2 Query: 713 FXXPPPPPXP 742 F PPPPP P Sbjct: 944 FPPPPPPPPP 953 Score = 21.8 bits (44), Expect(2) = 8.4 Identities = 7/9 (77%), Positives = 7/9 (77%) Frame = +2 Query: 722 PPPPPXPXL 748 PPPPP P L Sbjct: 946 PPPPPPPPL 954 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,837,650 Number of Sequences: 5004 Number of extensions: 25424 Number of successful extensions: 271 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 53 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 183 length of database: 2,362,478 effective HSP length: 73 effective length of database: 1,997,186 effective search space used: 547228964 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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