BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_L08
(1044 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 37 0.004
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 36 0.007
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.36
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 1.1
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 29 1.4
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 29 1.4
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 26 7.6
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 26 7.6
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 22 8.4
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 37.1 bits (82), Expect = 0.004
Identities = 18/38 (47%), Positives = 19/38 (50%)
Frame = +3
Query: 723 PPPPPXPXSXAXLPTPPPPXXRXSXLFXPPPPGPXGXP 836
PPPPP P S A P PP R + PPPP P P
Sbjct: 337 PPPPPPPRSNAAGSIPLPPQGRSA----PPPPPPRSAP 370
Score = 31.1 bits (67), Expect = 0.27
Identities = 13/33 (39%), Positives = 14/33 (42%)
Frame = +3
Query: 726 PPPPXPXSXAXLPTPPPPXXRXSXLFXPPPPGP 824
PPPP P S PPP + PP P P
Sbjct: 361 PPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPP 393
Score = 29.9 bits (64), Expect = 0.62
Identities = 13/33 (39%), Positives = 14/33 (42%)
Frame = +3
Query: 726 PPPPXPXSXAXLPTPPPPXXRXSXLFXPPPPGP 824
PPPP P S PP + PPPP P
Sbjct: 311 PPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPP 343
Score = 29.1 bits (62), Expect = 1.1
Identities = 17/49 (34%), Positives = 18/49 (36%), Gaps = 8/49 (16%)
Frame = +3
Query: 693 GGGSXLLXXXPPPP--------PXPXSXAXLPTPPPPXXRXSXLFXPPP 815
G + L PPPP P P P PPPP S PPP
Sbjct: 330 GSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPP 378
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 36.3 bits (80), Expect = 0.007
Identities = 15/34 (44%), Positives = 16/34 (47%)
Frame = +3
Query: 723 PPPPPXPXSXAXLPTPPPPXXRXSXLFXPPPPGP 824
P PPP P P PPPP + PPPP P
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 34.7 bits (76), Expect = 0.022
Identities = 18/45 (40%), Positives = 20/45 (44%), Gaps = 4/45 (8%)
Frame = +3
Query: 708 LLXXXPPPPP----XPXSXAXLPTPPPPXXRXSXLFXPPPPGPXG 830
LL PPPPP + A +P PPP PPPPG G
Sbjct: 728 LLKSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAG 772
Score = 31.1 bits (67), Expect = 0.27
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = +3
Query: 708 LLXXXPPPPPXPXSXAXLPTPPPP 779
++ PPPPP P P PPPP
Sbjct: 757 IMGGPPPPPPPPGVAGAGPPPPPP 780
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 30.7 bits (66), Expect = 0.36
Identities = 14/33 (42%), Positives = 15/33 (45%)
Frame = +3
Query: 723 PPPPPXPXSXAXLPTPPPPXXRXSXLFXPPPPG 821
PPPP P A P+ PPP S P PG
Sbjct: 1715 PPPPSAPPMPAGPPSAPPPPLPASSAPSVPNPG 1747
Score = 28.7 bits (61), Expect = 1.4
Identities = 16/35 (45%), Positives = 17/35 (48%), Gaps = 1/35 (2%)
Frame = +3
Query: 723 PPPPPXPXSXAXLPTPPP-PXXRXSXLFXPPPPGP 824
P PPP P S P+ PP P S PPPP P
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPS---APPPPLP 1736
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 29.1 bits (62), Expect = 1.1
Identities = 14/35 (40%), Positives = 16/35 (45%)
Frame = +3
Query: 732 PPXPXSXAXLPTPPPPXXRXSXLFXPPPPGPXGXP 836
PP P S + P+ PPP S P P P G P
Sbjct: 1050 PPVPKSSSGAPSAPPPVPAPSSEI-PSIPAPSGAP 1083
Score = 28.3 bits (60), Expect = 1.9
Identities = 15/40 (37%), Positives = 18/40 (45%), Gaps = 3/40 (7%)
Frame = +3
Query: 726 PPPPXPXSXAXLPTPP---PPXXRXSXLFXPPPPGPXGXP 836
PP P P +P P PP + S + PP P P G P
Sbjct: 1083 PPVPAPSGIPPVPKPSVAAPPVPKPS-VAVPPVPAPSGAP 1121
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 28.7 bits (61), Expect = 1.4
Identities = 15/33 (45%), Positives = 16/33 (48%)
Frame = +3
Query: 723 PPPPPXPXSXAXLPTPPPPXXRXSXLFXPPPPG 821
PPPPP P P+ PPP PPPPG
Sbjct: 9 PPPPPPPPGFEP-PSQPPP---------PPPPG 31
Score = 28.3 bits (60), Expect = 1.9
Identities = 11/23 (47%), Positives = 11/23 (47%)
Frame = +3
Query: 762 PTPPPPXXRXSXLFXPPPPGPXG 830
P PPPP PPPP P G
Sbjct: 9 PPPPPPPPGFEPPSQPPPPPPPG 31
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 28.7 bits (61), Expect = 1.4
Identities = 10/17 (58%), Positives = 10/17 (58%)
Frame = +3
Query: 723 PPPPPXPXSXAXLPTPP 773
PPPPP P A P PP
Sbjct: 6 PPPPPAPAPAAAAPAPP 22
Score = 27.1 bits (57), Expect = 4.4
Identities = 16/37 (43%), Positives = 16/37 (43%)
Frame = +3
Query: 726 PPPPXPXSXAXLPTPPPPXXRXSXLFXPPPPGPXGXP 836
P PP P S PT PP R S PPP P P
Sbjct: 128 PAPPTPQSELRPPTSAPP--RPSI----PPPSPASAP 158
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 26.2 bits (55), Expect = 7.6
Identities = 13/33 (39%), Positives = 16/33 (48%), Gaps = 3/33 (9%)
Frame = +3
Query: 723 PPPPPXPXSXAXLP---TPPPPXXRXSXLFXPP 812
PPPPP A P +PPPP + + PP
Sbjct: 908 PPPPPTASMTASAPAIASPPPP--KVGETYHPP 938
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 26.2 bits (55), Expect = 7.6
Identities = 16/42 (38%), Positives = 18/42 (42%)
Frame = +3
Query: 699 GSXLLXXXPPPPPXPXSXAXLPTPPPPXXRXSXLFXPPPPGP 824
GS L PPPP P + LP PP + PP GP
Sbjct: 1873 GSDLSNQAAPPPPPPMA---LPKAGPPSAAPTSAL--PPAGP 1909
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 22.2 bits (45), Expect(2) = 8.4
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = +2
Query: 713 FXXPPPPPXP 742
F PPPPP P
Sbjct: 944 FPPPPPPPPP 953
Score = 21.8 bits (44), Expect(2) = 8.4
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = +2
Query: 722 PPPPPXPXL 748
PPPPP P L
Sbjct: 946 PPPPPPPPL 954
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,837,650
Number of Sequences: 5004
Number of extensions: 25424
Number of successful extensions: 271
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 53
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 183
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 547228964
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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