BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_L07
(869 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 36 0.007
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 30 0.49
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 0.65
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 0.65
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 0.86
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 29 1.1
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 29 1.1
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 26 6.1
SPAC11H11.01 |sst6|cps23|ESCRT I complex subunit Vps23|Schizosac... 26 6.1
SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces ... 26 8.0
SPCC1739.06c |||uroporphyrin methyltransferase |Schizosaccharomy... 26 8.0
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 35.9 bits (79), Expect = 0.007
Identities = 20/57 (35%), Positives = 20/57 (35%)
Frame = +2
Query: 668 PXPPPXXXXXXXPPXXXPPPXXXXXPXQXXKXPPPXXPPPXPXXXXXXXXPPXPXPP 838
P PPP P P P P PPP PPP P PP P PP
Sbjct: 732 PPPPPPAVIVPTPA---PAPIPVPPPAPIMGGPPP--PPPPPGVAGAGPPPPPPPPP 783
Score = 34.7 bits (76), Expect = 0.017
Identities = 21/76 (27%), Positives = 21/76 (27%)
Frame = +2
Query: 614 PXXPXXXTXXXXXXPXXXPXPPPXXXXXXXPPXXXPPPXXXXXPXQXXKXPPPXXPPPXP 793
P P P P PPP PP PP P PPP PPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGP------PPPPPPPPAV 785
Query: 794 XXXXXXXXPPXPXPPP 841
P P P
Sbjct: 786 SAGGSRYYAPAPQAEP 801
Score = 27.9 bits (59), Expect = 2.0
Identities = 16/51 (31%), Positives = 16/51 (31%), Gaps = 5/51 (9%)
Frame = +2
Query: 719 PPPXXXXXPXQXXKXPPPXXPPPXPXXXXXXXXPPXP-----XPPPXXPXP 856
PPP P PPP P PP P PPP P P
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 27.1 bits (57), Expect = 3.5
Identities = 12/40 (30%), Positives = 13/40 (32%)
Frame = +2
Query: 668 PXPPPXXXXXXXPPXXXPPPXXXXXPXQXXKXPPPXXPPP 787
P PPP PP PPP + P P P
Sbjct: 764 PPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAPQAEPEP 803
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 29.9 bits (64), Expect = 0.49
Identities = 17/59 (28%), Positives = 17/59 (28%), Gaps = 1/59 (1%)
Frame = +2
Query: 677 PPXXXXXXXPPXXXPPPXXXXXPXQXXKXPPPXXPPPXPXXXXXXXXPPXP-XPPPXXP 850
PP PP PP P P P PP P P P PP P
Sbjct: 130 PPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAP 188
Score = 27.1 bits (57), Expect = 3.5
Identities = 18/54 (33%), Positives = 18/54 (33%), Gaps = 5/54 (9%)
Frame = +2
Query: 704 PPXXXPPPXXXXXPXQXXKXPP-PXXPPPXP--XXXXXXXXPPXP--XPPPXXP 850
PP PP PP P PPP P PP P PPP P
Sbjct: 124 PPSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQP 177
Score = 27.1 bits (57), Expect = 3.5
Identities = 18/81 (22%), Positives = 18/81 (22%)
Frame = +2
Query: 614 PXXPXXXTXXXXXXPXXXPXPPPXXXXXXXPPXXXPPPXXXXXPXQXXKXPPPXXPPPXP 793
P P P PPP P PP P P P
Sbjct: 130 PPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPS 189
Query: 794 XXXXXXXXPPXPXPPPXXPXP 856
PP PPP P
Sbjct: 190 LPSAVPPMPPKVPPPPLSQAP 210
Score = 26.2 bits (55), Expect = 6.1
Identities = 17/65 (26%), Positives = 18/65 (27%)
Frame = +3
Query: 672 PXPXXPXXXXXPPXXXXPXPXXXXXXXXXXXPPPXXPPPXPXXXXXXXXXPPPPXRXXXP 851
P P P PP P P P P PP P PPP + P
Sbjct: 128 PAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSL-------PPPAQPAAP 180
Query: 852 XPXXP 866
P
Sbjct: 181 VKSPP 185
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 29.5 bits (63), Expect = 0.65
Identities = 35/157 (22%), Positives = 37/157 (23%), Gaps = 10/157 (6%)
Frame = +2
Query: 410 PXXPPPK------IFFPXXGXXXXXPPPPXFWEXKXXFXLPXKXXXXXXXXXXPQXPXXP 571
P PPP+ I P G PPPP P P P P
Sbjct: 338 PPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPP-PAIP 396
Query: 572 XXXXPXXXXXXXXX----PXXPXXXTXXXXXXPXXXPXPPPXXXXXXXPPXXXPPPXXXX 739
P P P + P P PP PP
Sbjct: 397 GRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIA 456
Query: 740 XPXQXXKXPPPXXPPPXPXXXXXXXXPPXPXPPPXXP 850
P P PP P PP P P P P
Sbjct: 457 PPLPAGMPAAPPLPPAAP------APPPAPAPAPAAP 487
Score = 28.3 bits (60), Expect = 1.5
Identities = 19/67 (28%), Positives = 19/67 (28%), Gaps = 2/67 (2%)
Frame = +2
Query: 656 PXXXPXPPPXXXXXXXPPXXXPPP--XXXXXPXQXXKXPPPXXPPPXPXXXXXXXXPPXP 829
P P PPP PP P P P PP PPP
Sbjct: 251 PAPPPIPPPSNGTVSSPPNSPPRPIAPVSMNPAINSTSKPP-LPPPSSRVSAAALAANKK 309
Query: 830 XPPPXXP 850
PPP P
Sbjct: 310 RPPPPPP 316
Score = 28.3 bits (60), Expect = 1.5
Identities = 19/68 (27%), Positives = 19/68 (27%), Gaps = 2/68 (2%)
Frame = +2
Query: 668 PXPPPXXXXXXXPPXXXPPPXXXXXPXQXXKXPP--PXXPPPXPXXXXXXXXPPXPXPPP 841
P P P PP PP P PP P P P PP P P
Sbjct: 416 PVPTPPSLPPSAPPSL-PPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAP 474
Query: 842 XXPXPXXP 865
P P
Sbjct: 475 APPPAPAP 482
Score = 27.9 bits (59), Expect = 2.0
Identities = 17/66 (25%), Positives = 18/66 (27%)
Frame = +2
Query: 668 PXPPPXXXXXXXPPXXXPPPXXXXXPXQXXKXPPPXXPPPXPXXXXXXXXPPXPXPPPXX 847
P PPP PP P + P P P P P PPP
Sbjct: 337 PPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNP-PAPPPAI 395
Query: 848 PXPXXP 865
P P
Sbjct: 396 PGRSAP 401
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 29.5 bits (63), Expect = 0.65
Identities = 19/66 (28%), Positives = 19/66 (28%)
Frame = +2
Query: 668 PXPPPXXXXXXXPPXXXPPPXXXXXPXQXXKXPPPXXPPPXPXXXXXXXXPPXPXPPPXX 847
P P P PP PP PPP PP P PP P P
Sbjct: 1180 PVPKPAAGVPPVPPPSEAPP-VPKPSVGVPPVPPPSTAPPVP--TPSAGLPPVPVPTAKA 1236
Query: 848 PXPXXP 865
P P
Sbjct: 1237 PPVPAP 1242
Score = 28.3 bits (60), Expect = 1.5
Identities = 16/63 (25%), Positives = 16/63 (25%)
Frame = +2
Query: 668 PXPPPXXXXXXXPPXXXPPPXXXXXPXQXXKXPPPXXPPPXPXXXXXXXXPPXPXPPPXX 847
P P P P P PPP PP P P P P
Sbjct: 1160 PVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPV 1219
Query: 848 PXP 856
P P
Sbjct: 1220 PTP 1222
Score = 27.5 bits (58), Expect = 2.6
Identities = 17/63 (26%), Positives = 17/63 (26%), Gaps = 3/63 (4%)
Frame = +2
Query: 677 PPXXXXXXXPPXXXPPPXXXXXPXQXXKXPP---PXXPPPXPXXXXXXXXPPXPXPPPXX 847
PP PP P P PP P PP P P P P
Sbjct: 1083 PPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPV 1142
Query: 848 PXP 856
P P
Sbjct: 1143 PKP 1145
Score = 27.1 bits (57), Expect = 3.5
Identities = 17/66 (25%), Positives = 17/66 (25%), Gaps = 2/66 (3%)
Frame = +2
Query: 674 PPPXXXXXXXPPXXXPPPXXXXXPXQXXKXPPPXX--PPPXPXXXXXXXXPPXPXPPPXX 847
PP PP P P P P PP P P P P P
Sbjct: 1012 PPVPKLSSKAPPVPLPSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSS 1071
Query: 848 PXPXXP 865
P P
Sbjct: 1072 EIPSIP 1077
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 29.1 bits (62), Expect = 0.86
Identities = 12/32 (37%), Positives = 12/32 (37%)
Frame = +2
Query: 770 PXXPPPXPXXXXXXXXPPXPXPPPXXPXPXXP 865
P PPP PP P PP P P P
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
Score = 28.7 bits (61), Expect = 1.1
Identities = 18/61 (29%), Positives = 18/61 (29%), Gaps = 1/61 (1%)
Frame = +2
Query: 677 PPXXXXXXXPPXXXPPPXXXXXPXQXXKXPPPXXPPPXPXXXXXXXXPPXP-XPPPXXPX 853
PP PP P P PPP PP P PP P P P
Sbjct: 1690 PPVRPQSAAPPQMSAPTP----PPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPN 1745
Query: 854 P 856
P
Sbjct: 1746 P 1746
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 28.7 bits (61), Expect = 1.1
Identities = 11/26 (42%), Positives = 11/26 (42%)
Frame = +2
Query: 764 PPPXXPPPXPXXXXXXXXPPXPXPPP 841
PP PPP P P P PPP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPP 30
Score = 27.1 bits (57), Expect = 3.5
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = +2
Query: 767 PPXXPPPXPXXXXXXXXPPXPXPPPXXP 850
PP PPP P PP PPP P
Sbjct: 5 PPGNPPPPPPPPGFE--PPSQPPPPPPP 30
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 28.7 bits (61), Expect = 1.1
Identities = 24/70 (34%), Positives = 24/70 (34%), Gaps = 3/70 (4%)
Frame = -3
Query: 855 GXGXXGGGXGXGGXXXXXXXXGX-GGGXXG--GGXXXXXXGXXXXXGGGXXXGGXXXXXX 685
G G GG G GG G GGG G GG G GG GG
Sbjct: 203 GPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGG--GPGG 260
Query: 684 XGGGXGXXXG 655
GGG G G
Sbjct: 261 FGGGPGGHGG 270
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 26.2 bits (55), Expect = 6.1
Identities = 18/51 (35%), Positives = 18/51 (35%), Gaps = 2/51 (3%)
Frame = -3
Query: 849 GXXGGGXGXGGXXXXXXXXGXGGGXXG-GGXXXXXXGXXXXXGG-GXXXGG 703
G GG G GG G GG G GG G GG G GG
Sbjct: 20 GFNGGRGGFGGGRGGARGGGRGGARGGRGGRGGARGGRGGSSGGRGGAKGG 70
>SPAC11H11.01 |sst6|cps23|ESCRT I complex subunit
Vps23|Schizosaccharomyces pombe|chr 1|||Manual
Length = 487
Score = 26.2 bits (55), Expect = 6.1
Identities = 14/40 (35%), Positives = 20/40 (50%)
Frame = +2
Query: 149 VHAFVKRDAPKEDNSLNTLAESAKKTIEELREKVESRLGP 268
V A +K+D +E S L TIE+L+ K E+ P
Sbjct: 128 VQALIKQDFEREHTSPPELPTKLVNTIEKLKVKEENEAPP 167
>SPCC74.06 |mak3|phk2|histidine kinase Mak3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 2344
Score = 25.8 bits (54), Expect = 8.0
Identities = 13/50 (26%), Positives = 26/50 (52%)
Frame = -3
Query: 246 FSLNSSIVFFALSASVFRLLSSLGASRLTNACTLARQIANKIISSLFILD 97
F ++ SIV+ A+ ++V + + A ++ NK+ S L++LD
Sbjct: 417 FEVSQSIVYSAIVSAVAEFIRQILAEDQLLLNNFFEELKNKLESDLYLLD 466
>SPCC1739.06c |||uroporphyrin methyltransferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 496
Score = 25.8 bits (54), Expect = 8.0
Identities = 13/43 (30%), Positives = 22/43 (51%)
Frame = -3
Query: 237 NSSIVFFALSASVFRLLSSLGASRLTNACTLARQIANKIISSL 109
N S+ F L A+ + S +N C LA+++ ++SSL
Sbjct: 121 NPSLCSFTLPATWSEPPLQISLSTSSNGCRLAQRLLRHVVSSL 163
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,960,034
Number of Sequences: 5004
Number of extensions: 33698
Number of successful extensions: 312
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 85
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 434475230
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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