Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_K23
(869 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 33 0.070
SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 30 0.37
SPBC428.08c |clr4||histone H3 methyltransferase Clr4|Schizosacch... 30 0.49
SPCC584.15c |||arrestin/PY protein 2|Schizosaccharomyces pombe|c... 29 0.65
SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces ... 28 2.0
SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac... 28 2.0
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 27 3.5
SPBC31F10.13c |hip1|hir1|hira protein Hip1|Schizosaccharomyces p... 27 3.5
SPAC824.09c |||GTPase activating protein |Schizosaccharomyces po... 27 4.6
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 4.6
SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large... 26 6.1
SPAC4A8.11c |fas2|lsd1|fatty acid synthase alpha subunit Lsd1 |S... 26 6.1
SPBC16H5.11c |skb1|rmt5|type II protein arginine N-methyltransfe... 26 8.0
SPCC1739.14 |npp106||nucleoporin Npp106|Schizosaccharomyces pomb... 26 8.0
SPAC3G9.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 26 8.0
SPCC18B5.03 |wee1||dual specificity protein kinase Wee1|Schizosa... 26 8.0
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 32.7 bits (71), Expect = 0.070
Identities = 17/63 (26%), Positives = 29/63 (46%)
Frame = +1
Query: 352 SPTSLTRTVTNPREAHLPTPHPIPEAIARALAYIRGPPPQPLRRGKKSRRQLVRISQDNN 531
+P S+ + + + LP P A A A R PPP P R + + + S +++
Sbjct: 276 APVSMNPAINSTSKPPLPPPSSRVSAAALAANKKRPPPPPPPSRRNRGKPPIGNGSSNSS 335
Query: 532 IPP 540
+PP
Sbjct: 336 LPP 338
Score = 28.7 bits (61), Expect = 1.1
Identities = 25/102 (24%), Positives = 40/102 (39%), Gaps = 5/102 (4%)
Frame = +3
Query: 213 SSSTTRPATEFTPRLKVPSRTSTQNTP-PSKLRVP---TSTLPLDGQPIDLAYVADENGY 380
+S++ P P + P R + + P P + P T + P + P +A V+
Sbjct: 225 TSTSAPPIPPSIPSSRPPERVPSLSAPAPPPIPPPSNGTVSSPPNSPPRPIAPVSMNPAI 284
Query: 381 QPQGSPSAHPSPNSRGDRPRSCLHS-RATPPAPPSWKEKSSP 503
P P P+SR + R PP PPS + + P
Sbjct: 285 NSTSKPPL-PPPSSRVSAAALAANKKRPPPPPPPSRRNRGKP 325
Score = 28.3 bits (60), Expect = 1.5
Identities = 25/78 (32%), Positives = 30/78 (38%)
Frame = +3
Query: 249 PRLKVPSRTSTQNTPPSKLRVPTSTLPLDGQPIDLAYVADENGYQPQGSPSAHPSPNSRG 428
P L S +TPP VPT P L A + P G+P+A P P S
Sbjct: 401 PALPPLGNASRTSTPP----VPTPPSLPPSAPPSLPPSAPPS--LPMGAPAAPPLPPSAP 454
Query: 429 DRPRSCLHSRATPPAPPS 482
P A PP PP+
Sbjct: 455 IAPPLPAGMPAAPPLPPA 472
Score = 26.6 bits (56), Expect = 4.6
Identities = 13/38 (34%), Positives = 18/38 (47%)
Frame = +2
Query: 395 PICPPLTQFPRRSPALLPTFEGHPPSPSVVERKVVANL 508
PI PPL +P L P PP+P+ VA++
Sbjct: 454 PIAPPLPAGMPAAPPLPPAAPAPPPAPAPAPAAPVASI 491
>SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1752
Score = 30.3 bits (65), Expect = 0.37
Identities = 32/106 (30%), Positives = 42/106 (39%), Gaps = 4/106 (3%)
Frame = +3
Query: 201 WKATSSSTTRPATEFTPRLKVPSRTSTQNTPPSKLRVPTSTLPLDGQPIDLAYVADENGY 380
+ ATS S + + ++P S TS +P S PTS P +Y Y
Sbjct: 1606 YSATSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP---SYSPTSPSYSPTSPSY 1662
Query: 381 QPQGSPSAHP-SPNSRGDRPRSCLHSRATPPAPPSWKEKS---SPT 506
P SPS P SP+ P S + P PS+ S SPT
Sbjct: 1663 SPT-SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPT 1707
Score = 27.1 bits (57), Expect = 3.5
Identities = 32/99 (32%), Positives = 39/99 (39%), Gaps = 4/99 (4%)
Frame = +3
Query: 222 TTRPATEFTPRLKVPSRTSTQNTPPSKLRVPTSTLPLDGQPIDLAYVADENGYQPQGSPS 401
+T PA + P S TS +P S PTS P +Y A Y P SPS
Sbjct: 1566 STSPA--YMPSSPSYSPTSPSYSPTSPSYSPTSP---SYSPTSPSYSATSPSYSPT-SPS 1619
Query: 402 AHP-SPNSRGDRPRSCLHSRATPPAPPSWKEKS---SPT 506
P SP+ P S + P PS+ S SPT
Sbjct: 1620 YSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPT 1658
>SPBC428.08c |clr4||histone H3 methyltransferase
Clr4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 490
Score = 29.9 bits (64), Expect = 0.49
Identities = 23/66 (34%), Positives = 33/66 (50%), Gaps = 8/66 (12%)
Frame = +3
Query: 399 SAHPSPNSRGDR--------PRSCLHSRATPPAPPSWKEKSSPTC*DKSRQQHTATSTNE 554
+++P PNSR PRS SR + K SS T +SR+Q TA +TN+
Sbjct: 77 ASNPHPNSRQKHQHQTSKSVPRSQRFSRELNVKKENKKVFSSQTTKRQSRKQSTALTTND 136
Query: 555 TDIMXE 572
T I+ +
Sbjct: 137 TSIILD 142
>SPCC584.15c |||arrestin/PY protein 2|Schizosaccharomyces pombe|chr
3|||Manual
Length = 594
Score = 29.5 bits (63), Expect = 0.65
Identities = 15/40 (37%), Positives = 19/40 (47%)
Frame = +1
Query: 346 STSPTSLTRTVTNPREAHLPTPHPIPEAIARALAYIRGPP 465
+T P+S T +V P P P+P IA AL PP
Sbjct: 532 ATRPSSPTESVEIPSNTTTIAPSPVPTIIAPALPSTPAPP 571
>SPCC16C4.07 |scw1||RNA-binding protein Scw1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 561
Score = 27.9 bits (59), Expect = 2.0
Identities = 21/76 (27%), Positives = 35/76 (46%), Gaps = 3/76 (3%)
Frame = +3
Query: 264 PSRTST---QNTPPSKLRVPTSTLPLDGQPIDLAYVADENGYQPQGSPSAHPSPNSRGDR 434
PS++ST Q+ P LR + ++ + + Y++ E GY S+ SP+ R
Sbjct: 305 PSKSSTNLRQDGVPPILRFNSLSINTN---VARNYLSSEKGYSAHTQNSSAQSPHPRVFS 361
Query: 435 PRSCLHSRATPPAPPS 482
S + + PP PS
Sbjct: 362 ANSAFSTTSPPPLTPS 377
>SPCC736.14 |dis1||microtubule-associated protein Dis1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 882
Score = 27.9 bits (59), Expect = 2.0
Identities = 21/68 (30%), Positives = 30/68 (44%)
Frame = +3
Query: 294 PSKLRVPTSTLPLDGQPIDLAYVADENGYQPQGSPSAHPSPNSRGDRPRSCLHSRATPPA 473
PS+ R+P PL P+ LA P PS++PS S + S S T P+
Sbjct: 541 PSRPRLPRVASPLKTSPVKLAVTPQ----APSPLPSSNPSQASLTEESLS-TRSSPTKPS 595
Query: 474 PPSWKEKS 497
S + +S
Sbjct: 596 TTSLRSQS 603
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 27.1 bits (57), Expect = 3.5
Identities = 28/113 (24%), Positives = 43/113 (38%), Gaps = 3/113 (2%)
Frame = +1
Query: 142 IAKSDEYAAPVVKSSYDITPG--RPLPVQLRDRQRNLRPG*RCRQERQLRIPRHRS*GCL 315
+ ++ Y +P +S ITP PLP Q +P R+ +
Sbjct: 826 VPRTTSYTSPYATTSSHITPADVHPLPPPSTSTTAGWNDAPMLGQ-----LPMRRAAPSM 880
Query: 316 Q-VHFPWTDNPSTSPTSLTRTVTNPREAHLPTPHPIPEAIARALAYIRGPPPQ 471
V P+ S P +++RT + + LP P P A A A PPP+
Sbjct: 881 APVRSPFPGASSAQPAAMSRTSS---VSTLPPPPPTASMTASAPAIASPPPPK 930
>SPBC31F10.13c |hip1|hir1|hira protein Hip1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 932
Score = 27.1 bits (57), Expect = 3.5
Identities = 16/44 (36%), Positives = 20/44 (45%), Gaps = 2/44 (4%)
Frame = +3
Query: 222 TTRPATEFTPRLKVPS--RTSTQNTPPSKLRVPTSTLPLDGQPI 347
TT AT T RL T + PP + P ++LP G PI
Sbjct: 493 TTLSATPSTSRLASTQLQHTGSSQLPPQQFSQPINSLPKGGVPI 536
>SPAC824.09c |||GTPase activating protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 320
Score = 26.6 bits (56), Expect = 4.6
Identities = 27/87 (31%), Positives = 34/87 (39%), Gaps = 16/87 (18%)
Frame = +3
Query: 246 TPRLKVPSRTSTQNTPPSKLRVPTSTLPLDGQPIDL---------AYVADE------NGY 380
+PR +V S + T N L P ST QP ++VAD+
Sbjct: 225 SPRPQVSSSSITTNATYQNLPSPVSTSTTSSQPYGAFHQPATTGSSFVADKWKMPMFTSN 284
Query: 381 QPQGSPSAHPSP-NSRGDRPRSCLHSR 458
PSA SP S RPRS L S+
Sbjct: 285 VTSAQPSARASPVQSNSSRPRSSLDSK 311
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 26.6 bits (56), Expect = 4.6
Identities = 11/30 (36%), Positives = 14/30 (46%)
Frame = +1
Query: 385 PREAHLPTPHPIPEAIARALAYIRGPPPQP 474
P +PTP P P + + GPPP P
Sbjct: 736 PPAVIVPTPAPAPIPVPPPAPIMGGPPPPP 765
>SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large
subunit Rpc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1405
Score = 26.2 bits (55), Expect = 6.1
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +3
Query: 246 TPRLKVPSRTSTQNTPPSKLRVPTST 323
TP+LK+PS+ T N ++ V TS+
Sbjct: 1169 TPKLKIPSQQVTVNNTLQQIHVHTSS 1194
>SPAC4A8.11c |fas2|lsd1|fatty acid synthase alpha subunit Lsd1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1842
Score = 26.2 bits (55), Expect = 6.1
Identities = 22/83 (26%), Positives = 34/83 (40%), Gaps = 8/83 (9%)
Frame = +1
Query: 337 DNPSTSPTSLTRTV---TNPREAHLPTPHPIPEAIARALAYIRGPPPQPLR-----RGKK 492
D P+ +P S + T T A TP P P + A + PP+ L +K
Sbjct: 99 DEPAEAPASTSSTPKVETAAAAAPAATPAPAPAQTSAPAAALPDEPPKALEVLHTLVAQK 158
Query: 493 SRRQLVRISQDNNIPPLVQTKLT 561
++ + +S +I LV K T
Sbjct: 159 LKKSIEEVSPQKSIKDLVGGKST 181
>SPBC16H5.11c |skb1|rmt5|type II protein arginine
N-methyltransferase Skb1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 645
Score = 25.8 bits (54), Expect = 8.0
Identities = 18/58 (31%), Positives = 27/58 (46%), Gaps = 1/58 (1%)
Frame = +3
Query: 222 TTRPATEFTPRLKVPSRTSTQNTPPSKLRVPTSTLPLDGQPID-LAYVADENGYQPQG 392
T R A + PRLKV +PP +L P++ + +A+V + NGY G
Sbjct: 181 TIRSACGYHPRLKVALELPPACSPPIELVNRWYAEPIEMITMSCMAFVPNPNGYPVLG 238
>SPCC1739.14 |npp106||nucleoporin Npp106|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 933
Score = 25.8 bits (54), Expect = 8.0
Identities = 12/22 (54%), Positives = 13/22 (59%)
Frame = +1
Query: 169 PVVKSSYDITPGRPLPVQLRDR 234
P+ SY P PLP QLRDR
Sbjct: 427 PLYFYSYAKNPSLPLPKQLRDR 448
>SPAC3G9.01 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 462
Score = 25.8 bits (54), Expect = 8.0
Identities = 13/46 (28%), Positives = 26/46 (56%)
Frame = +3
Query: 267 SRTSTQNTPPSKLRVPTSTLPLDGQPIDLAYVADENGYQPQGSPSA 404
S +ST +PPS++++PTS L P+ + + + P +P++
Sbjct: 199 SLSSTIKSPPSRVKLPTSILS-KSPPLKVPNKNRSSTFSPLRTPTS 243
>SPCC18B5.03 |wee1||dual specificity protein kinase
Wee1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 877
Score = 25.8 bits (54), Expect = 8.0
Identities = 14/51 (27%), Positives = 26/51 (50%)
Frame = +3
Query: 171 SGQIQLRHHPWKATSSSTTRPATEFTPRLKVPSRTSTQNTPPSKLRVPTST 323
+G + +H P K + + P+T P V +S+ ++PPS P++T
Sbjct: 383 TGLLSKQHRPRKNINFTPLPPSTPSKPSTFVRPHSSSTDSPPSP-STPSNT 432
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,463,110
Number of Sequences: 5004
Number of extensions: 48899
Number of successful extensions: 224
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 198
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 221
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 434475230
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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