Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_J21
(884 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 36 0.006
SPBC29A3.09c |||AAA family ATPase Gcn20 |Schizosaccharomyces pom... 33 0.071
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 31 0.22
SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3 Brl2|Schizosa... 30 0.38
SPBP8B7.11 |nxt3||ubiquitin protease cofactor |Schizosaccharomyc... 30 0.50
SPAC4F10.11 |spn1||septin Spn1|Schizosaccharomyces pombe|chr 1||... 30 0.50
SPAC16E8.06c |nop12||RNA-binding protein Nop12|Schizosaccharomyc... 29 0.88
SPAC139.06 |hat1|SPAC23C4.01|histone acetyltransferase Hat1|Schi... 29 1.2
SPAC6F6.12 |||autophagy associated protein Atg24|Schizosaccharom... 28 1.5
SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 28 1.5
SPCC63.11 |prp28||U5 snRNP-associated protein Prp28 |Schizosacch... 28 2.0
SPAC16E8.11c |tfb1||transcription factor TFIIH complex subunit T... 28 2.0
SPCC10H11.01 |prp11||ATP-dependent RNA helicase Prp11|Schizosacc... 27 2.7
SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schiz... 27 3.6
SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 27 4.7
SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting pro... 26 6.2
SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 26 6.2
SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|ch... 26 8.2
SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 26 8.2
SPAC6F12.08c |||exocyst complex subunit Exo84|Schizosaccharomyce... 26 8.2
>SPAC17C9.03 |tif471||translation initiation factor eIF4G
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1403
Score = 36.3 bits (80), Expect = 0.006
Identities = 26/91 (28%), Positives = 47/91 (51%)
Frame = +1
Query: 178 IEYSADLSALSRLEDKIRLIQDDLESERELRQRIEREKADLSVQVIQLSERLEEAEGGAE 357
I+ A+ A + E+K RL ++ ++RE ++ +RE + + + + + EAE A+
Sbjct: 537 IQEKAEAEAKRKAEEKARLEAEE-NAKREAEEQAKREAEEKAKREAEEKAK-REAEEKAK 594
Query: 358 SQFEINRKRDTELLKLRKLLEDVHLESEETA 450
+ E N KR+ E R+ E E+EE A
Sbjct: 595 REAEENAKREAEEKAKREAEEKAKREAEEKA 625
Score = 30.3 bits (65), Expect = 0.38
Identities = 23/87 (26%), Positives = 44/87 (50%)
Frame = +1
Query: 190 ADLSALSRLEDKIRLIQDDLESERELRQRIEREKADLSVQVIQLSERLEEAEGGAESQFE 369
A+ A E+K + ++ +++RE ++ +RE + + + + + EAE A+ + E
Sbjct: 613 AEEKAKREAEEKAKREAEE-KAKREAEEKAKREAEEKAKREAEEKAK-REAEENAKREAE 670
Query: 370 INRKRDTELLKLRKLLEDVHLESEETA 450
KR+ E R+ E V E+EE A
Sbjct: 671 EKAKREAEENAKREAEEKVKRETEENA 697
Score = 29.1 bits (62), Expect = 0.88
Identities = 23/92 (25%), Positives = 45/92 (48%)
Frame = +1
Query: 175 NIEYSADLSALSRLEDKIRLIQDDLESERELRQRIEREKADLSVQVIQLSERLEEAEGGA 354
N + A+ A E+K + ++ +++RE ++ +RE + + + + + EAE A
Sbjct: 560 NAKREAEEQAKREAEEKAKREAEE-KAKREAEEKAKREAEENAKREAEEKAK-REAEEKA 617
Query: 355 ESQFEINRKRDTELLKLRKLLEDVHLESEETA 450
+ + E KR+ E R+ E E+EE A
Sbjct: 618 KREAEEKAKREAEEKAKREAEEKAKREAEEKA 649
Score = 28.3 bits (60), Expect = 1.5
Identities = 21/85 (24%), Positives = 45/85 (52%)
Frame = +1
Query: 190 ADLSALSRLEDKIRLIQDDLESERELRQRIEREKADLSVQVIQLSERLEEAEGGAESQFE 369
A+ A E+K + ++ +++RE ++ +RE + + + + + + EAE A+ + E
Sbjct: 621 AEEKAKREAEEKAKREAEE-KAKREAEEKAKREAEEKAKREAEENAK-REAEEKAKREAE 678
Query: 370 INRKRDTELLKLRKLLEDVHLESEE 444
N KR+ E R+ E+ ++EE
Sbjct: 679 ENAKREAEEKVKRETEENAKRKAEE 703
Score = 26.6 bits (56), Expect = 4.7
Identities = 21/85 (24%), Positives = 44/85 (51%)
Frame = +1
Query: 190 ADLSALSRLEDKIRLIQDDLESERELRQRIEREKADLSVQVIQLSERLEEAEGGAESQFE 369
A+ A E+K + ++ +++RE ++ +RE + + + + + EAE A+ + E
Sbjct: 629 AEEKAKREAEEKAKREAEE-KAKREAEEKAKREAEENAKREAEEKAK-REAEENAKREAE 686
Query: 370 INRKRDTELLKLRKLLEDVHLESEE 444
KR+TE RK E+ E+++
Sbjct: 687 EKVKRETEENAKRKAEEEGKREADK 711
>SPBC29A3.09c |||AAA family ATPase Gcn20 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 736
Score = 32.7 bits (71), Expect = 0.071
Identities = 14/54 (25%), Positives = 34/54 (62%), Gaps = 1/54 (1%)
Frame = +1
Query: 205 LSRLEDKIRLIQDDLESERELRQRIEREKADLSVQVIQLSERLEEAEGG-AESQ 363
LS +E ++ + D +++ + +R+ERE+ +L ++++ + +L E + AES+
Sbjct: 280 LSTIEKELEELSKDQTADQAISRRLERERDELDLRLLDIQNKLSEMDSDRAESR 333
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 31.1 bits (67), Expect = 0.22
Identities = 19/88 (21%), Positives = 47/88 (53%)
Frame = +1
Query: 223 KIRLIQDDLESERELRQRIEREKADLSVQVIQLSERLEEAEGGAESQFEINRKRDTELLK 402
+++ +++++++ +E +E+ S+Q +L+E L+ ++ E Q ++ K ELL
Sbjct: 607 QLKSLREEIDNTKEALDLSVKER---SIQEEKLNESLKTSKTNLEEQTQLAEKYHEELLD 663
Query: 403 LRKLLEDVHLESEETAXLLKXXTXEIVI 486
++ L D+ +E + T K E+ +
Sbjct: 664 NQQKLYDLRIELDYTKSNCKQMEEEMQV 691
>SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3
Brl2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 680
Score = 30.3 bits (65), Expect = 0.38
Identities = 16/61 (26%), Positives = 32/61 (52%)
Frame = +1
Query: 163 TTDVNIEYSADLSALSRLEDKIRLIQDDLESERELRQRIEREKADLSVQVIQLSERLEEA 342
+T ++ S+ S + LE+K+ +Q ++ +E + +DLS + LS++ EE
Sbjct: 540 STTFQVKKSSQKSTIQNLEEKVSYLQQFMDKNNATLTDLEFQCSDLSSSIDILSKQDEEH 599
Query: 343 E 345
E
Sbjct: 600 E 600
>SPBP8B7.11 |nxt3||ubiquitin protease cofactor |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 434
Score = 29.9 bits (64), Expect = 0.50
Identities = 16/48 (33%), Positives = 26/48 (54%), Gaps = 4/48 (8%)
Frame = +1
Query: 214 LEDKIRLIQDDLESERELRQRIEREKADLS----VQVIQLSERLEEAE 345
L D R +++D+E E E +E+EK D++ V +Q E L A+
Sbjct: 128 LNDIFRFLREDVEEEEESPDAVEKEKKDVASEPYVNGVQSQEHLPSAK 175
>SPAC4F10.11 |spn1||septin Spn1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 469
Score = 29.9 bits (64), Expect = 0.50
Identities = 16/50 (32%), Positives = 32/50 (64%), Gaps = 1/50 (2%)
Frame = +1
Query: 217 EDKIRLIQDDLESE-RELRQRIEREKADLSVQVIQLSERLEEAEGGAESQ 363
ED+++ +++L + RE++ +E++KADL I RL +A+ AE++
Sbjct: 416 EDRLKQSENELRTRHREMKAALEKQKADL----IDHKNRLMQAKAAAENE 461
>SPAC16E8.06c |nop12||RNA-binding protein Nop12|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 438
Score = 29.1 bits (62), Expect = 0.88
Identities = 21/61 (34%), Positives = 35/61 (57%), Gaps = 2/61 (3%)
Frame = +1
Query: 172 VNIEYSADLSALSRLEDKIRLIQDDLESERELRQRI--EREKADLSVQVIQLSERLEEAE 345
+N E + S LE+ R Q+D++SERE+ +++ E EK+D +V V L R+ +
Sbjct: 122 INDEEDKSPAKQSVLEE--RTSQEDVKSEREVAEKLANELEKSDKTVFVNNLPARVVTNK 179
Query: 346 G 348
G
Sbjct: 180 G 180
>SPAC139.06 |hat1|SPAC23C4.01|histone acetyltransferase
Hat1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 378
Score = 28.7 bits (61), Expect = 1.2
Identities = 15/49 (30%), Positives = 31/49 (63%)
Frame = +1
Query: 265 LRQRIEREKADLSVQVIQLSERLEEAEGGAESQFEINRKRDTELLKLRK 411
+++RI R+ D+ +Q + SER+E+ E+QF+ ++ +L KL++
Sbjct: 317 IKERIFRQNLDVLLQ-LDKSERIEKIHNAYENQFDEYKQIVKKLPKLKE 364
>SPAC6F6.12 |||autophagy associated protein
Atg24|Schizosaccharomyces pombe|chr 1|||Manual
Length = 401
Score = 28.3 bits (60), Expect = 1.5
Identities = 22/78 (28%), Positives = 35/78 (44%), Gaps = 4/78 (5%)
Frame = +1
Query: 160 GTTDVNIEY----SADLSALSRLEDKIRLIQDDLESERELRQRIEREKADLSVQVIQLSE 327
G+ D ++ Y +LS +R E K+R D +E+ R+ D V +
Sbjct: 31 GSRDTHVSYLIITKTNLSIFTRAECKVRRRFSDFVKLQEILSRMNE---DCVVPPLPAKH 87
Query: 328 RLEEAEGGAESQFEINRK 381
+LE +GG S INR+
Sbjct: 88 KLEYIKGGRFSDNFINRR 105
>SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1190
Score = 28.3 bits (60), Expect = 1.5
Identities = 20/64 (31%), Positives = 30/64 (46%), Gaps = 1/64 (1%)
Frame = +1
Query: 229 RLIQDDLESERELRQRIEREKADLSVQVIQLSERLEEAEGGAESQFEINRKRDTEL-LKL 405
R QD+ ESE E R + + ++ V ++E +G SQ +R R T L +
Sbjct: 131 RRSQDEEESEEEHRPILRERTSRINYSVPLAFPPVDEMDGDPSSQVNQSRSRKTHSELAI 190
Query: 406 RKLL 417
KLL
Sbjct: 191 TKLL 194
>SPCC63.11 |prp28||U5 snRNP-associated protein Prp28
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 27.9 bits (59), Expect = 2.0
Identities = 25/74 (33%), Positives = 37/74 (50%), Gaps = 3/74 (4%)
Frame = +1
Query: 238 QDDLESERELRQ--RIEREKADLSVQVIQLSERLEEA-EGGAESQFEINRKRDTELLKLR 408
QD + S +L Q R++ EKA + + +ER A E + E K++ +LL LR
Sbjct: 4 QDSIPSLEQLVQQKRVKEEKA-ARPKFLSKAERARLALERRQKEVEEAKAKQNDKLLDLR 62
Query: 409 KLLEDVHLESEETA 450
K HLE+ E A
Sbjct: 63 KRTFTNHLENNELA 76
>SPAC16E8.11c |tfb1||transcription factor TFIIH complex subunit
Tfb1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 477
Score = 27.9 bits (59), Expect = 2.0
Identities = 20/71 (28%), Positives = 33/71 (46%)
Frame = +1
Query: 250 ESERELRQRIEREKADLSVQVIQLSERLEEAEGGAESQFEINRKRDTELLKLRKLLEDVH 429
E ++ RI++E + QLSERL E G +F N + + LK D+
Sbjct: 238 ELRPDITMRIDKEALPFMKNINQLSERLLEKSLGNSKRF--NNENEETYLK-ESGFHDLE 294
Query: 430 LESEETAXLLK 462
E+ ++ +LK
Sbjct: 295 EEASDSKVVLK 305
>SPCC10H11.01 |prp11||ATP-dependent RNA helicase
Prp11|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1014
Score = 27.5 bits (58), Expect = 2.7
Identities = 24/84 (28%), Positives = 44/84 (52%)
Frame = +1
Query: 193 DLSALSRLEDKIRLIQDDLESERELRQRIEREKADLSVQVIQLSERLEEAEGGAESQFEI 372
D L+RLE ++R ++ ++++E ++ E K + QV + + G S FEI
Sbjct: 192 DAKLLARLE-RVRAWKES-KAKQEASKK-EEHKLNTKPQVTAKDQNAMPSTG--ISGFEI 246
Query: 373 NRKRDTELLKLRKLLEDVHLESEE 444
NR++DT +K VH++ E+
Sbjct: 247 NRQKDTSDMKRN---NRVHMDDED 267
>SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 527
Score = 27.1 bits (57), Expect = 3.6
Identities = 16/73 (21%), Positives = 33/73 (45%)
Frame = +1
Query: 184 YSADLSALSRLEDKIRLIQDDLESERELRQRIEREKADLSVQVIQLSERLEEAEGGAESQ 363
+ +L LE+K++L + + + R+E E DL+ + + + R+ A +
Sbjct: 76 FDEELVKFHNLEEKLQLTETKCRNAESEKSRVENELEDLTSSLFEEANRM---VANARKE 132
Query: 364 FEINRKRDTELLK 402
+ KR +L K
Sbjct: 133 TVASEKRVNQLKK 145
>SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1526
Score = 26.6 bits (56), Expect = 4.7
Identities = 24/107 (22%), Positives = 48/107 (44%), Gaps = 3/107 (2%)
Frame = +1
Query: 133 KYTYRSSGGGTTDVN---IEYSADLSALSRLEDKIRLIQDDLESERELRQRIEREKADLS 303
KY + ++V +E + L+A+ L R I D E E+ +R + A++
Sbjct: 837 KYELKKQQNSKSEVERDLVETNNSLTAVENLLTTERAIALDKE---EILRRTQERLANIE 893
Query: 304 VQVIQLSERLEEAEGGAESQFEINRKRDTELLKLRKLLEDVHLESEE 444
+ ++ E + + S +IN + ++ELL+ +E + E E
Sbjct: 894 DSFSETKQQNENLQRESASLKQINNELESELLEKTSKVETLLSEQNE 940
Score = 26.2 bits (55), Expect = 6.2
Identities = 17/73 (23%), Positives = 34/73 (46%), Gaps = 1/73 (1%)
Frame = +1
Query: 205 LSRLEDKIRLIQDDLESERELRQRIEREKADLSVQVIQLSERLEEAEGGAESQF-EINRK 381
L R ++++ I+D ++ + ++RE A L +L L E E+ E N
Sbjct: 882 LRRTQERLANIEDSFSETKQQNENLQRESASLKQINNELESELLEKTSKVETLLSEQNEL 941
Query: 382 RDTELLKLRKLLE 420
++ L+ + LL+
Sbjct: 942 KEKLSLEEKDLLD 954
>SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting
protein 3 homolog Bud6|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1385
Score = 26.2 bits (55), Expect = 6.2
Identities = 18/66 (27%), Positives = 35/66 (53%), Gaps = 2/66 (3%)
Frame = +1
Query: 112 SAMAKTSKYTYRSSGGGTTDV--NIEYSADLSALSRLEDKIRLIQDDLESERELRQRIER 285
S+ +K + + G T + +I + + +A +LED ++ D S+REL +R +
Sbjct: 448 SSTLSVNKKQFNADDGSTLNSPNSIRETEEYAASPKLEDIADEVETDATSQRELLER-QI 506
Query: 286 EKADLS 303
+KA+ S
Sbjct: 507 QKAESS 512
>SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 547
Score = 26.2 bits (55), Expect = 6.2
Identities = 20/80 (25%), Positives = 37/80 (46%), Gaps = 4/80 (5%)
Frame = +1
Query: 148 SSGGGTTDVNIEYSADLSALSRLEDKIRLIQDDLESERELRQRIEREKA---DLSVQVIQ 318
+S TD+ S L + +DK++ + LE L+ E EK+ +L V++
Sbjct: 403 ASSNANTDLRSRVDISESKLKKRDDKLKRVSSQLE---HLKHNYEEEKSMNENLLVRIQT 459
Query: 319 L-SERLEEAEGGAESQFEIN 375
L + +++ QF+IN
Sbjct: 460 LEKQNTTKSDQIVSMQFQIN 479
>SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1208
Score = 25.8 bits (54), Expect = 8.2
Identities = 18/85 (21%), Positives = 38/85 (44%)
Frame = +1
Query: 181 EYSADLSALSRLEDKIRLIQDDLESERELRQRIEREKADLSVQVIQLSERLEEAEGGAES 360
+Y+ + D+I+++ DLE E+E Q + +A + + L+E A
Sbjct: 346 DYAILQAKCDEFADRIQVLTADLEKEKE-NQIMHESEASIGLTDSMQVHTLQEQLHKANE 404
Query: 361 QFEINRKRDTELLKLRKLLEDVHLE 435
+ E + + + + K ED+ L+
Sbjct: 405 EIEFLHDQISRMNEEGKNFEDIMLQ 429
>SPBC17D11.05 |tif32||translation initiation factor
eIF3a|Schizosaccharomyces pombe|chr 2|||Manual
Length = 932
Score = 25.8 bits (54), Expect = 8.2
Identities = 22/80 (27%), Positives = 40/80 (50%), Gaps = 5/80 (6%)
Frame = +1
Query: 190 ADLSALSRLEDKIRLIQDDLESERELR----QRIEREKADLSV-QVIQLSERLEEAEGGA 354
A+L+A L+ K + L + E+ +RI REK + + + +L+E L +A+GG
Sbjct: 583 AELAAQRALKQKQESEAESLRVQEEINKRNAERIRREKEAIRINEAKKLAEEL-KAKGGL 641
Query: 355 ESQFEINRKRDTELLKLRKL 414
E E D + L+ ++
Sbjct: 642 EVNAEDLEHLDADKLRAMQI 661
>SPAC6F12.08c |||exocyst complex subunit Exo84|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 578
Score = 25.8 bits (54), Expect = 8.2
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +1
Query: 205 LSRLEDKIRLIQDDLESERELRQRIEREKADL 300
L LE+ R +DLE REL + E+AD+
Sbjct: 302 LQELENWSRKHDEDLEFSRELEYHTKSEQADM 333
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,205,277
Number of Sequences: 5004
Number of extensions: 30283
Number of successful extensions: 177
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 168
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 444486180
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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