Bombyx BLAST Result against tribolium

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP03_F_J17
         (884 letters)

Database: tribolium 
           336 sequences; 122,585 total letters

Searching.......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF322227-1|AAK01654.1|  782|Tribolium castaneum cell surface pro...    52   6e-09
AJ829922-1|CAH25640.1|  193|Tribolium castaneum twist bHLH trans...    21   9.7  

>AF322227-1|AAK01654.1|  782|Tribolium castaneum cell surface
           protein chaoptin protein.
          Length = 782

 Score = 52.0 bits (119), Expect = 6e-09
 Identities = 44/133 (33%), Positives = 66/133 (49%), Gaps = 5/133 (3%)
 Frame = +1

Query: 145 QVLDLSECQLMQVPDAVYHLMRHTE-LKSCDLSGNVITKIPPKFAVKFSLITDLNLSNNQ 321
           QVLDLS   L ++    +   R+T+ L+  D S N I++IP         +  ++ S+N+
Sbjct: 275 QVLDLSHNSLYELD---FDTFRNTKKLQWLDTSHNRISEIPNDLFRFLGNLRIVDFSHNR 331

Query: 322 MARLPDELCTLACLQRLDISHNTFVALPHITFQCPSLHTLL---AHHNQIIEVD-VDRLA 489
           +  LPD L     L+RLD+SHN    LP  +    S  TL       N I  +    +LA
Sbjct: 332 LRSLPDNLFRETGLERLDVSHNLLGKLPLTSLSLASAQTLSELDLSWNSISSLSHGGQLA 391

Query: 490 RSQALEYVDLSDN 528
           R + L ++DLS N
Sbjct: 392 RFKCLSWLDLSYN 404



 Score = 34.3 bits (75), Expect = 0.001
 Identities = 32/111 (28%), Positives = 50/111 (45%), Gaps = 8/111 (7%)
 Frame = +1

Query: 265 PKFAVKF-SLITDLNLSNNQMARLPD-ELCTLACLQRLDISHNTFVALPHITFQCP---S 429
           P  A+K  + + +L+LSNN++  +PD     L  L+++ +  NT   +   TFQ      
Sbjct: 34  PSDAIKILNRLEELDLSNNRLRNVPDNSFHFLRSLKKVHLQDNTIEMIHRGTFQGDIHRD 93

Query: 430 LHTLLAHHNQIIEVDVDRLARSQALEYVDLSDNPIPA---RTHDDLKQLSR 573
           L  +    N +  V     A    LE + L DN I +   R   +LK L R
Sbjct: 94  LTEVYFSFNSVRNVQQHTFADLIQLEQIHLDDNRIESLERRAFMNLKSLKR 144



 Score = 33.5 bits (73), Expect = 0.002
 Identities = 19/65 (29%), Positives = 31/65 (47%), Gaps = 1/65 (1%)
 Frame = +1

Query: 361 LQRLDISHNTFV-ALPHITFQCPSLHTLLAHHNQIIEVDVDRLARSQALEYVDLSDNPIP 537
           L +L + HN  + A   +    P L  L   HN + E+D D    ++ L+++D S N I 
Sbjct: 250 LMQLYLGHNKLLNATKDLFGNMPHLQVLDLSHNSLYELDFDTFRNTKKLQWLDTSHNRIS 309

Query: 538 ARTHD 552
              +D
Sbjct: 310 EIPND 314



 Score = 32.7 bits (71), Expect = 0.004
 Identities = 36/132 (27%), Positives = 54/132 (40%), Gaps = 4/132 (3%)
 Frame = +1

Query: 151 LDLSECQLMQVPDAVYHLMRHTELKSCDLSGNVITKI-PPKFAVKFSL-ITDLNLSNNQM 324
           LDLS  +L  VPD  +H +R   LK   L  N I  I    F       +T++  S N +
Sbjct: 47  LDLSNNRLRNVPDNSFHFLR--SLKKVHLQDNTIEMIHRGTFQGDIHRDLTEVYFSFNSV 104

Query: 325 ARLPDE-LCTLACLQRLDISHNTFVALPHITF-QCPSLHTLLAHHNQIIEVDVDRLARSQ 498
             +       L  L+++ +  N   +L    F    SL  L    N+I  +  +      
Sbjct: 105 RNVQQHTFADLIQLEQIHLDDNRIESLERRAFMNLKSLKRLNLKGNKIATIAYETFQNLP 164

Query: 499 ALEYVDLSDNPI 534
            LE +DL+ N I
Sbjct: 165 ELEDLDLAYNSI 176



 Score = 25.8 bits (54), Expect = 0.45
 Identities = 35/120 (29%), Positives = 55/120 (45%), Gaps = 14/120 (11%)
 Frame = +1

Query: 217 ELKSCDLSGNVITKIPPK-FAVKFSL-ITDLNLSNNQMARL------PDELCT-LACLQR 369
           EL+  DL+ N I+ +    F    SL +  +N+S+N++  L      P E  T L  LQ 
Sbjct: 165 ELEDLDLAYNSISSLDFNIFDQVGSLGMFHVNMSHNKLINLVVAPSVPFEQDTGLGGLQN 224

Query: 370 ---LDISHNTFVALPHITFQCP--SLHTLLAHHNQIIEVDVDRLARSQALEYVDLSDNPI 534
              LD+S N   ++    F+    SL  L   HN+++    D       L+ +DLS N +
Sbjct: 225 IKVLDLSFNNITSVAKQFFRPVELSLMQLYLGHNKLLNATKDLFGNMPHLQVLDLSHNSL 284



 Score = 24.2 bits (50), Expect = 1.4
 Identities = 43/169 (25%), Positives = 65/169 (38%), Gaps = 10/169 (5%)
 Frame = +1

Query: 118 LRCEDAQETQVLDLSECQLMQVPDAVYHLMRHTELKSCDLSGNVITKIPPKFAVKFSLIT 297
           L    AQ    LDLS   +  +      L R   L   DLS N + +I          + 
Sbjct: 363 LSLASAQTLSELDLSWNSISSLSHGG-QLARFKCLSWLDLSYNRLGQIDAGTFKGIPRLA 421

Query: 298 DLNLSNNQMARLPDELCTLACLQRLDISHNTF-VALPHI-TFQCPSLHTLLAHHNQIIEV 471
            LNL +N    L     +   L+   +  N   V+L  +     P+L +L    N +  V
Sbjct: 422 SLNLGHNSQLTLEINGLSFQGLEYTLLHLNLDNVSLSQVPALSTPNLLSLSLAFNSLPTV 481

Query: 472 DVDRLARSQALEYV-----DLSDNPIPARTHDDLKQLS---RPSVTLSD 594
            ++      +L Y+     DLS  PI   +  +L+ LS    P  TLS+
Sbjct: 482 ALEVAGNISSLRYLNLDYNDLSAVPIVTHSLTELRHLSLEGNPITTLSN 530


>AJ829922-1|CAH25640.1|  193|Tribolium castaneum twist bHLH
           transcription factor protein.
          Length = 193

 Score = 21.4 bits (43), Expect = 9.7
 Identities = 8/25 (32%), Positives = 14/25 (56%)
 Frame = +1

Query: 187 DAVYHLMRHTELKSCDLSGNVITKI 261
           D +YH++ +      DL GNV + +
Sbjct: 145 DFLYHVLSNENALDVDLIGNVCSYV 169


  Database: tribolium
    Posted date:  Oct 23, 2007  1:18 PM
  Number of letters in database: 122,585
  Number of sequences in database:  336
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 192,828
Number of Sequences: 336
Number of extensions: 4167
Number of successful extensions: 12
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 10
length of database: 122,585
effective HSP length: 57
effective length of database: 103,433
effective search space used: 24513621
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)