Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_J10
(867 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 30 0.49
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 29 0.65
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 29 0.86
SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 29 1.1
SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch... 28 2.0
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 27 2.6
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 4.6
SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation... 26 6.0
SPAC11D3.08c |||amino acid permease, unknown 1|Schizosaccharomyc... 26 8.0
>SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 168
Score = 29.9 bits (64), Expect = 0.49
Identities = 13/46 (28%), Positives = 25/46 (54%)
Frame = +2
Query: 296 KDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEE 433
KDG+++ +LN FAK L + + +A + + IE+++ E
Sbjct: 111 KDGTDAFANELNLFAKKLGFSKNSFDARALDTESEDETEIEKSSSE 156
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 29.5 bits (63), Expect = 0.65
Identities = 20/76 (26%), Positives = 34/76 (44%), Gaps = 3/76 (3%)
Frame = +2
Query: 152 PKVAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLT---KSKDAQDFSKAWKDGSESVLQ 322
P V ++ DFFK + ++ H TL ++ NSL+ +K + G +
Sbjct: 50 PYVGNRKKSEQDFFKMLSSRDRDAHSTLRKRSNSLSSFLSTKSTSASENKFHGGLNWLSL 109
Query: 323 QLNAFAKSLQGALGDA 370
+LN + LQG + A
Sbjct: 110 KLNLLLR-LQGRMNSA 124
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 29.1 bits (62), Expect = 0.86
Identities = 23/89 (25%), Positives = 40/89 (44%)
Frame = +2
Query: 155 KVAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNA 334
K V R F + +EH+ K+LE+Q + L +SKDA A S+ +
Sbjct: 195 KSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQSKDASSNLTACNRSGSSLSSNFYS 253
Query: 335 FAKSLQGALGDANGKAKEALEQSRQNIER 421
S + +L N K++ +L+ ++ R
Sbjct: 254 SRLSKKTSLASLN-KSRASLQHKIMSLSR 281
>SPBC646.09c |int6|yin6|translation initiation factor
eIF3e|Schizosaccharomyces pombe|chr 2|||Manual
Length = 501
Score = 28.7 bits (61), Expect = 1.1
Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
Frame = +2
Query: 275 QDFSKAWKDGSESVLQQLNAFAKSLQGALGD-ANGKAKEALEQSR-QNIERTAE 430
+D + A+ + SVLQ+L+ + +QG LG N AL Q + QN++ E
Sbjct: 79 EDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALRQDKGQNLQHLQE 132
>SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 803
Score = 27.9 bits (59), Expect = 2.0
Identities = 24/74 (32%), Positives = 33/74 (44%), Gaps = 6/74 (8%)
Frame = +2
Query: 230 TLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGA------LGDANGKAKEA 391
T++ + SL K D + ++ ES L L L A L D+ KAK
Sbjct: 354 TIQIELESLRKETDTTSVER--REKLESKLTDLKEEQDKLSAAWEEERKLLDSIKKAKTE 411
Query: 392 LEQSRQNIERTAEE 433
LEQ+R +ERT E
Sbjct: 412 LEQARIELERTQRE 425
>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 631
Score = 27.5 bits (58), Expect = 2.6
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -2
Query: 407 ASTVPKPPWPCRSRLRALPG 348
AST+ K PWP + L +PG
Sbjct: 38 ASTLEKEPWPASTALLVMPG 57
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.6 bits (56), Expect = 4.6
Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
Frame = +2
Query: 176 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 295
D +F D++ H K FH E+ + + +K D K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706
>SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation
factor eIF2 beta subunit|Schizosaccharomyces pombe|chr
1|||Manual
Length = 321
Score = 26.2 bits (55), Expect = 6.0
Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
Frame = +2
Query: 212 TKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQ-GALGDA 370
+K+ + E+Q +T DFS K + L+AF K L+ + GDA
Sbjct: 89 SKKSSASAEEQTEDITTESGELDFSSMKKKKKKKKSADLSAFEKELEASSTGDA 142
>SPAC11D3.08c |||amino acid permease, unknown 1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 550
Score = 25.8 bits (54), Expect = 8.0
Identities = 20/60 (33%), Positives = 29/60 (48%), Gaps = 4/60 (6%)
Frame = +1
Query: 16 THYREFLRFDTLLCV-AVRFASPPHSVSRQYIMAAKF---VVLFACIALAQGSDGATRRS 183
T Y ++ + LL V + A P +++ I+ F V + IALA GSD TR S
Sbjct: 170 TKYEQYGIYAALLFVISAMTAIPSRVIAKVNIINITFQFLVSIILIIALAAGSDSTTRNS 229
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,360,362
Number of Sequences: 5004
Number of extensions: 35564
Number of successful extensions: 135
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 432473040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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