Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_J05
(914 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 35 0.014
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 32 0.099
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 32 0.099
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 31 0.17
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 0.92
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 3.7
SPCC330.10 |pcm1||mRNA capping methyltransferase|Schizosaccharom... 27 4.9
SPAC25B8.12c |||nucleotide-sugar phosphatase |Schizosaccharomyce... 26 6.5
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 35.1 bits (77), Expect = 0.014
Identities = 20/80 (25%), Positives = 20/80 (25%)
Frame = +3
Query: 672 PXPPPPXXXXXXPXPXXPXXXXXXXXXPXPPPPPXPXXXXXXXXPPXXXPXXPXPPXPXX 851
P PPPP P P PPP P P PP P
Sbjct: 361 PPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTP 420
Query: 852 XXXXXXXPXXXPXXXPPXXP 911
P P PP P
Sbjct: 421 PSLPPSAPPSLPPSAPPSLP 440
Score = 31.9 bits (69), Expect = 0.13
Identities = 17/56 (30%), Positives = 17/56 (30%)
Frame = +3
Query: 672 PXPPPPXXXXXXPXPXXPXXXXXXXXXPXPPPPPXPXXXXXXXXPPXXXPXXPXPP 839
P PPPP P P PPPPP PP P PP
Sbjct: 311 PPPPPPPSRRNRGKPPI-GNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPP 365
Score = 28.3 bits (60), Expect = 1.6
Identities = 14/38 (36%), Positives = 14/38 (36%)
Frame = +2
Query: 671 PPPPPPXXXXXXPXPXXPXXXXXXPXXPXXPPPPXXXP 784
PPPPPP P P P PPPP P
Sbjct: 338 PPPPPPRSNAAGSIPLPPQGRSAPP-----PPPPRSAP 370
Score = 27.9 bits (59), Expect = 2.1
Identities = 14/41 (34%), Positives = 14/41 (34%), Gaps = 1/41 (2%)
Frame = +2
Query: 665 PXPPPPPPXXXXXXPXP-XXPXXXXXXPXXPXXPPPPXXXP 784
P PP PP P P P P P PP P P
Sbjct: 444 PAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPAP 484
Score = 27.1 bits (57), Expect = 3.7
Identities = 18/63 (28%), Positives = 18/63 (28%), Gaps = 2/63 (3%)
Frame = +3
Query: 663 PXXPXPP--PPXXXXXXPXPXXPXXXXXXXXXPXPPPPPXPXXXXXXXXPPXXXPXXPXP 836
P P PP PP P P P P PP P P P P
Sbjct: 415 PPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAP-PLPPSAPIAPPLPAGMPAAPPLPPAA 473
Query: 837 PXP 845
P P
Sbjct: 474 PAP 476
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 32.3 bits (70), Expect = 0.099
Identities = 19/80 (23%), Positives = 19/80 (23%)
Frame = +3
Query: 663 PXXPXPPPPXXXXXXPXPXXPXXXXXXXXXPXPPPPPXPXXXXXXXXPPXXXPXXPXPPX 842
P P PP P P P PP P PP P P
Sbjct: 125 PSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSP 184
Query: 843 PXXXXXXXXXPXXXPXXXPP 902
P P P PP
Sbjct: 185 PSAPSLPSAVPPMPPKVPPP 204
Score = 25.8 bits (54), Expect = 8.6
Identities = 19/81 (23%), Positives = 19/81 (23%), Gaps = 1/81 (1%)
Frame = +1
Query: 664 PXXPXPP-PXXXARXPXXXXXXXXXXXXXXXXXPPPPXXXPXXXXXPXPXXXXXXPPXPX 840
P P PP P R P PP P P P P
Sbjct: 125 PSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSP 184
Query: 841 XPXPXXXXXXXPXXXPXPPPP 903
P P PPPP
Sbjct: 185 PSAPSLPSAVPPMPPKVPPPP 205
Score = 25.8 bits (54), Expect = 8.6
Identities = 11/34 (32%), Positives = 11/34 (32%)
Frame = +2
Query: 671 PPPPPPXXXXXXPXPXXPXXXXXXPXXPXXPPPP 772
PPP P P P P PPPP
Sbjct: 172 PPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPPPP 205
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 32.3 bits (70), Expect = 0.099
Identities = 13/34 (38%), Positives = 13/34 (38%)
Frame = +3
Query: 672 PXPPPPXXXXXXPXPXXPXXXXXXXXXPXPPPPP 773
P PPP P P P P PPPPP
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 31.1 bits (67), Expect = 0.23
Identities = 13/34 (38%), Positives = 13/34 (38%)
Frame = +2
Query: 671 PPPPPPXXXXXXPXPXXPXXXXXXPXXPXXPPPP 772
PPPPPP P P P PPPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPP 765
Score = 30.7 bits (66), Expect = 0.30
Identities = 15/51 (29%), Positives = 15/51 (29%)
Frame = +1
Query: 760 PPPPXXXPXXXXXPXPXXXXXXPPXPXXPXPXXXXXXXPXXXPXPPPPXXP 912
PPPP P P P P P P PPPP P
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 30.7 bits (66), Expect = 0.30
Identities = 13/36 (36%), Positives = 13/36 (36%)
Frame = +3
Query: 672 PXPPPPXXXXXXPXPXXPXXXXXXXXXPXPPPPPXP 779
P P PP P P P PPPPP P
Sbjct: 748 PIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 28.3 bits (60), Expect = 1.6
Identities = 14/50 (28%), Positives = 14/50 (28%)
Frame = +1
Query: 763 PPPXXXPXXXXXPXPXXXXXXPPXPXXPXPXXXXXXXPXXXPXPPPPXXP 912
PPP P P PP P P PPPP P
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 27.1 bits (57), Expect = 3.7
Identities = 12/36 (33%), Positives = 12/36 (33%)
Frame = +2
Query: 665 PXPPPPPPXXXXXXPXPXXPXXXXXXPXXPXXPPPP 772
P PPPP P P P PPPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPP 767
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 31.5 bits (68), Expect = 0.17
Identities = 24/83 (28%), Positives = 24/83 (28%)
Frame = -2
Query: 910 GXXGGXXXGXXXGXXXXXXXXXGXGGXGXXGXXXGGXXXXXXXXGXGGGGGXGXXXXXXX 731
G GG G G G GG G G G G GGG G
Sbjct: 184 GHNGGGFGGFGGGSGGPPP---GPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGF 240
Query: 730 XXGXXGXGXXXXXXGGGGXGXXG 662
G G G GGG G G
Sbjct: 241 GGGPGGFGGGLGGFGGGPGGFGG 263
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 29.1 bits (62), Expect = 0.92
Identities = 13/32 (40%), Positives = 13/32 (40%), Gaps = 1/32 (3%)
Frame = +3
Query: 753 PXPPPPPXPXXXXXXXXP-PXXXPXXPXPPXP 845
P PPPPP P P P P PP P
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
Score = 27.5 bits (58), Expect = 2.8
Identities = 14/40 (35%), Positives = 14/40 (35%)
Frame = +2
Query: 665 PXPPPPPPXXXXXXPXPXXPXXXXXXPXXPXXPPPPXXXP 784
P PPPPP P P P P P P P P
Sbjct: 1705 PTPPPPP---MSVPPPPSAPPMPAGPPSAPPPPLPASSAP 1741
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.1 bits (57), Expect = 3.7
Identities = 16/59 (27%), Positives = 16/59 (27%), Gaps = 1/59 (1%)
Frame = +3
Query: 663 PXXPXPPPPXXXXXXPXPXXPXXXXXXXXXPXPPPP-PXPXXXXXXXXPPXXXPXXPXP 836
P PP P P P P PP P P PP P P P
Sbjct: 1164 PSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTP 1222
Score = 26.6 bits (56), Expect = 4.9
Identities = 18/75 (24%), Positives = 18/75 (24%)
Frame = +3
Query: 678 PPPPXXXXXXPXPXXPXXXXXXXXXPXPPPPPXPXXXXXXXXPPXXXPXXPXPPXPXXXX 857
PP P P P P P P PP P PP P
Sbjct: 1050 PPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVP--KP 1107
Query: 858 XXXXXPXXXPXXXPP 902
P P PP
Sbjct: 1108 SVAVPPVPAPSGAPP 1122
Score = 26.2 bits (55), Expect = 6.5
Identities = 19/83 (22%), Positives = 19/83 (22%), Gaps = 3/83 (3%)
Frame = +3
Query: 672 PXPPPPXXXXXXPXPXX---PXXXXXXXXXPXPPPPPXPXXXXXXXXPPXXXPXXPXPPX 842
P P P P P P P PPP P P P PP
Sbjct: 1160 PVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPV 1219
Query: 843 PXXXXXXXXXPXXXPXXXPPXXP 911
P P P P
Sbjct: 1220 PTPSAGLPPVPVPTAKAPPVPAP 1242
>SPCC330.10 |pcm1||mRNA capping
methyltransferase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 389
Score = 26.6 bits (56), Expect = 4.9
Identities = 14/40 (35%), Positives = 19/40 (47%)
Frame = -3
Query: 348 IYGSRFKDHFPIALAEPKDLDRRTRFDDAVTDVPAHFFNF 229
IY RF + P + P + +DAVTDVP + F
Sbjct: 278 IYKVRFPESPPRSFRPPYGIQYYFYLEDAVTDVPEYVVPF 317
>SPAC25B8.12c |||nucleotide-sugar phosphatase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 303
Score = 26.2 bits (55), Expect = 6.5
Identities = 14/40 (35%), Positives = 20/40 (50%)
Frame = -3
Query: 330 KDHFPIALAEPKDLDRRTRFDDAVTDVPAHFFNFLEDLPP 211
K+ PIA+ + K + R + AV +V FN ED P
Sbjct: 259 KNGMPIAIEKAKAVSRVGNEEGAVGEVLERIFNIPEDYTP 298
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,306,779
Number of Sequences: 5004
Number of extensions: 42077
Number of successful extensions: 309
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 93
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 464508080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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