BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_I24
(1096 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 40 8e-04
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 35 0.017
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 34 0.030
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M... 32 0.16
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 31 0.28
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 31 0.28
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 31 0.37
SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex p... 28 2.0
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 3.5
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 27 4.6
SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc... 24 4.9
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 6.1
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 39.5 bits (88), Expect = 8e-04
Identities = 28/67 (41%), Positives = 28/67 (41%), Gaps = 1/67 (1%)
Frame = -1
Query: 391 RGGGGGERXXXGGGGXPPPXGXGGXXFXRGGGGEXGXAXGRRXGXGXXRXKXPXGF-XEX 215
RG GG GGG PP G GG G GGE G G G G P GF
Sbjct: 183 RGHNGGGFGGFGGGSGGPPPGPGGFGGFGGFGGE-GHHHGGHGGFGG----GPGGFEGGP 237
Query: 214 XGXGGGP 194
G GGGP
Sbjct: 238 GGFGGGP 244
Score = 36.7 bits (81), Expect = 0.006
Identities = 25/67 (37%), Positives = 25/67 (37%), Gaps = 1/67 (1%)
Frame = -1
Query: 394 GRGGGGGERXXXGGGGXPP-PXGXGGXXFXRGGGGEXGXAXGRRXGXGXXRXKXPXGFXE 218
G GGG G GG PP P G GG G G G G G G P GF
Sbjct: 184 GHNGGGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEG-GPGGFGG 242
Query: 217 XXGXGGG 197
G GG
Sbjct: 243 GPGGFGG 249
Score = 35.5 bits (78), Expect = 0.013
Identities = 26/72 (36%), Positives = 26/72 (36%), Gaps = 9/72 (12%)
Frame = -1
Query: 382 GGGERXXXGGGGXPPPX--------GXGGXXFXRGGGGEXGXAXGRRXGXGXXRXKXPXG 227
GGG GG G PPP G GG GG G G G G P G
Sbjct: 187 GGGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGG 246
Query: 226 F-XEXXGXGGGP 194
F G GGGP
Sbjct: 247 FGGGLGGFGGGP 258
Score = 33.1 bits (72), Expect = 0.070
Identities = 26/72 (36%), Positives = 26/72 (36%)
Frame = -1
Query: 409 PXXXXGRGGGGGERXXXGGGGXPPPXGXGGXXFXRGGGGEXGXAXGRRXGXGXXRXKXPX 230
P G GG GGE GG G G G F GG G G G G P
Sbjct: 204 PGGFGGFGGFGGEGHHHGGHGG---FGGGPGGF-EGGPGGFGGGPGGFGGGLGGFGGGPG 259
Query: 229 GFXEXXGXGGGP 194
GF G GGP
Sbjct: 260 GFGGGPGGHGGP 271
Score = 29.9 bits (64), Expect = 0.65
Identities = 12/22 (54%), Positives = 12/22 (54%)
Frame = -3
Query: 638 GGXGGLGGGXXGPPXPPXXXRG 573
GG GG GGG GPP P G
Sbjct: 188 GGFGGFGGGSGGPPPGPGGFGG 209
Score = 29.1 bits (62), Expect = 1.1
Identities = 15/37 (40%), Positives = 15/37 (40%)
Frame = -3
Query: 701 GGAXRAGGXGXGGRGXXXXXXGGXGGLGGGXXGPPXP 591
GG GG G G GG GG GGG G P
Sbjct: 235 GGPGGFGGGPGGFGGGLGGFGGGPGGFGGGPGGHGGP 271
Score = 28.7 bits (61), Expect = 1.5
Identities = 13/27 (48%), Positives = 13/27 (48%)
Frame = -3
Query: 683 GGXGXGGRGXXXXXXGGXGGLGGGXXG 603
G G GG G GG GG GGG G
Sbjct: 206 GFGGFGGFGGEGHHHGGHGGFGGGPGG 232
Score = 28.7 bits (61), Expect = 1.5
Identities = 14/33 (42%), Positives = 14/33 (42%)
Frame = -3
Query: 701 GGAXRAGGXGXGGRGXXXXXXGGXGGLGGGXXG 603
GG GG G G GG GG GGG G
Sbjct: 221 GGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGG 253
Score = 28.7 bits (61), Expect = 1.5
Identities = 19/48 (39%), Positives = 19/48 (39%), Gaps = 1/48 (2%)
Frame = -1
Query: 394 GRGGGGGERXXXGGGGXPPPXGXGGXXFXRGGG-GEXGXAXGRRXGXG 254
G GGG G GG P G GG GGG G G G G G
Sbjct: 225 GFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGPGGHGGPG 272
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 35.1 bits (77), Expect = 0.017
Identities = 21/47 (44%), Positives = 22/47 (46%), Gaps = 2/47 (4%)
Frame = -1
Query: 394 GRGG-GGGERXXXGGGGXPPPXGXGGXXFXRGG-GGEXGXAXGRRXG 260
GRGG GGG GGG G GG RGG GG G G + G
Sbjct: 24 GRGGFGGGRGGARGGGRGGARGGRGGRGGARGGRGGSSGGRGGAKGG 70
Score = 34.3 bits (75), Expect = 0.030
Identities = 26/67 (38%), Positives = 26/67 (38%), Gaps = 2/67 (2%)
Frame = -1
Query: 391 RGGGGGERXXXGGGGXPPPXGXGGXXFXRGG--GGEXGXAXGRRXGXGXXRXKXPXGFXE 218
RGG GG R GG G GG RGG GG G A G R G G R
Sbjct: 8 RGGRGGSRGGRGGFN----GGRGGFGGGRGGARGGGRGGARGGRGGRGGARGGRGGSSGG 63
Query: 217 XXGXGGG 197
G GG
Sbjct: 64 RGGAKGG 70
Score = 31.1 bits (67), Expect = 0.28
Identities = 16/34 (47%), Positives = 16/34 (47%), Gaps = 2/34 (5%)
Frame = -3
Query: 698 GAXRAGGXGX--GGRGXXXXXXGGXGGLGGGXXG 603
G R GG G GGRG GG GG GG G
Sbjct: 33 GGARGGGRGGARGGRGGRGGARGGRGGSSGGRGG 66
Score = 29.1 bits (62), Expect = 1.1
Identities = 14/27 (51%), Positives = 14/27 (51%)
Frame = -3
Query: 683 GGXGXGGRGXXXXXXGGXGGLGGGXXG 603
GG G G RG GG GG GGG G
Sbjct: 9 GGRG-GSRGGRGGFNGGRGGFGGGRGG 34
Score = 26.2 bits (55), Expect = 8.1
Identities = 13/33 (39%), Positives = 13/33 (39%)
Frame = -3
Query: 701 GGAXRAGGXGXGGRGXXXXXXGGXGGLGGGXXG 603
GG G GGRG GG G GG G
Sbjct: 37 GGGRGGARGGRGGRGGARGGRGGSSGGRGGAKG 69
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 34.3 bits (75), Expect = 0.030
Identities = 13/24 (54%), Positives = 13/24 (54%)
Frame = +1
Query: 616 PPNPPXPPXXXXXXPRPPXPXPPA 687
PP PP PP P PP P PPA
Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPPPA 784
Score = 33.9 bits (74), Expect = 0.040
Identities = 15/29 (51%), Positives = 15/29 (51%), Gaps = 4/29 (13%)
Frame = +3
Query: 321 PPXPXGGGXPPPP----XXXLSPPPPPRP 395
PP P GG PPPP PPPPP P
Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 29.5 bits (63), Expect = 0.87
Identities = 15/35 (42%), Positives = 15/35 (42%), Gaps = 5/35 (14%)
Frame = +1
Query: 328 PPXGGXXPPPPXXX-----SPPPPPPAXNXXXGAS 417
P GG PPPP PPPPPP G S
Sbjct: 756 PIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGS 790
Score = 27.9 bits (59), Expect = 2.6
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = +1
Query: 349 PPPPXXXSPPPPPP 390
PP P PPPPPP
Sbjct: 753 PPAPIMGGPPPPPP 766
Score = 27.5 bits (58), Expect = 3.5
Identities = 12/29 (41%), Positives = 12/29 (41%)
Frame = +3
Query: 324 PXPXGGGXPPPPXXXLSPPPPPRPXXXXG 410
P P PPP PPPPP P G
Sbjct: 744 PAPAPIPVPPPAPIMGGPPPPPPPPGVAG 772
Score = 27.1 bits (57), Expect = 4.6
Identities = 11/25 (44%), Positives = 11/25 (44%)
Frame = +1
Query: 349 PPPPXXXSPPPPPPAXNXXXGASLP 423
PPP PPPPP GA P
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPP 776
>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
1|||Manual
Length = 500
Score = 31.9 bits (69), Expect = 0.16
Identities = 16/30 (53%), Positives = 16/30 (53%)
Frame = -3
Query: 701 GGAXRAGGXGXGGRGXXXXXXGGXGGLGGG 612
GG R G G GGRG GG GG GGG
Sbjct: 446 GGGSRGGRGGFGGRG----GFGGRGGFGGG 471
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 31.1 bits (67), Expect = 0.28
Identities = 13/26 (50%), Positives = 14/26 (53%), Gaps = 2/26 (7%)
Frame = +1
Query: 613 PPPNPPXPPXXXXXXP--RPPXPXPP 684
PP NPP PP P +PP P PP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPP 30
Score = 28.7 bits (61), Expect = 1.5
Identities = 12/24 (50%), Positives = 12/24 (50%), Gaps = 2/24 (8%)
Frame = +3
Query: 330 PXGGGXPPPPXXXLSPP--PPPRP 395
P G PPPP PP PPP P
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPP 28
Score = 28.7 bits (61), Expect = 1.5
Identities = 11/24 (45%), Positives = 11/24 (45%)
Frame = +1
Query: 604 PXXPPPNPPXPPXXXXXXPRPPXP 675
P PPP PP P P PP P
Sbjct: 6 PGNPPPPPPPPGFEPPSQPPPPPP 29
Score = 27.1 bits (57), Expect = 4.6
Identities = 13/28 (46%), Positives = 13/28 (46%)
Frame = +3
Query: 312 NXXPPXPXGGGXPPPPXXXLSPPPPPRP 395
N PP P G PP PPPPP P
Sbjct: 8 NPPPPPPPPGFEPPS-----QPPPPPPP 30
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 31.1 bits (67), Expect = 0.28
Identities = 11/25 (44%), Positives = 14/25 (56%)
Frame = +3
Query: 321 PPXPXGGGXPPPPXXXLSPPPPPRP 395
PP P G PP P ++ PP P+P
Sbjct: 1083 PPVPAPSGIPPVPKPSVAAPPVPKP 1107
Score = 29.9 bits (64), Expect = 0.65
Identities = 11/25 (44%), Positives = 13/25 (52%)
Frame = +3
Query: 321 PPXPXGGGXPPPPXXXLSPPPPPRP 395
PP P G PP P ++ PP P P
Sbjct: 1131 PPVPVPSGAPPVPKPSVAAPPVPAP 1155
Score = 29.9 bits (64), Expect = 0.65
Identities = 11/25 (44%), Positives = 13/25 (52%)
Frame = +3
Query: 321 PPXPXGGGXPPPPXXXLSPPPPPRP 395
PP P G PP P ++ PP P P
Sbjct: 1150 PPVPAPSGAPPVPKPSVAAPPVPAP 1174
Score = 29.1 bits (62), Expect = 1.1
Identities = 11/25 (44%), Positives = 13/25 (52%)
Frame = +3
Query: 321 PPXPXGGGXPPPPXXXLSPPPPPRP 395
PP P G PP P ++ PP P P
Sbjct: 1112 PPVPAPSGAPPVPKPSVAAPPVPVP 1136
Score = 26.6 bits (56), Expect = 6.1
Identities = 18/64 (28%), Positives = 21/64 (32%), Gaps = 4/64 (6%)
Frame = +2
Query: 146 PXPAAFSXPGXXLFXLGXPPXPXXLXKPXXXFXXXRSXPXXAPXRXPXLPPP----PPXK 313
P PA P + PP P P + P AP P +P P PP
Sbjct: 1113 PVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVP 1172
Query: 314 XPXS 325
P S
Sbjct: 1173 APSS 1176
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 30.7 bits (66), Expect = 0.37
Identities = 25/104 (24%), Positives = 25/104 (24%)
Frame = +1
Query: 373 PPPPPPAXNXXXGASLPHXXXXXXXXXXXXXXXXXXRXXXXXXXXXXXXXXXCXPPXPRG 552
PPPPPP N LP R PP G
Sbjct: 338 PPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPG 397
Query: 553 XRXXAXLPLXXXGGXGGPXXPPPNPPXPPXXXXXXPRPPXPXPP 684
A PL P P P P PP P P P
Sbjct: 398 RSAPALPPLGNASRTSTPPVPTP-PSLPPSAPPSLPPSAPPSLP 440
Score = 28.7 bits (61), Expect = 1.5
Identities = 29/127 (22%), Positives = 30/127 (23%), Gaps = 7/127 (5%)
Frame = +1
Query: 337 GGXXPPPPXXXSPPPPPPAXNXXXGASLPHXXXXXXXXXXXXXXXXXXRXXXXXXXXXXX 516
G PP +PPPPPP G P
Sbjct: 349 GSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGN 408
Query: 517 XXXXCXPPXPRGXRXXAXLP--LXXXGGXGGPXXPPPNPPXPPXXXXXXPRP-----PXP 675
PP P P L P P PP PP P P P
Sbjct: 409 ASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPP 468
Query: 676 XPPARXA 696
PPA A
Sbjct: 469 LPPAAPA 475
Score = 27.1 bits (57), Expect = 4.6
Identities = 19/59 (32%), Positives = 19/59 (32%), Gaps = 5/59 (8%)
Frame = +1
Query: 535 PPXPRGXRXXAXLPLXXXGGXGGPXXPPPNPPXPPXXXXXXPRPP-----XPXPPARXA 696
PP P R P G PPP PP P PP P PP R A
Sbjct: 312 PPPPPPSRRNRGKPPIGNGSSNSSLPPPP-PPPRSNAAGSIPLPPQGRSAPPPPPPRSA 369
Score = 27.1 bits (57), Expect = 4.6
Identities = 12/26 (46%), Positives = 13/26 (50%), Gaps = 1/26 (3%)
Frame = +3
Query: 321 PPXPXG-GGXPPPPXXXLSPPPPPRP 395
PP P G PP P +PPP P P
Sbjct: 457 PPLPAGMPAAPPLPPAAPAPPPAPAP 482
Score = 26.6 bits (56), Expect = 6.1
Identities = 14/33 (42%), Positives = 14/33 (42%), Gaps = 2/33 (6%)
Frame = +3
Query: 321 PPXPXGGGXPPPPXXXLS--PPPPPRPXXXXGR 413
PP G P P S PPPPPR GR
Sbjct: 342 PPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGR 374
>SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex
protein Gar1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 194
Score = 28.3 bits (60), Expect = 2.0
Identities = 23/59 (38%), Positives = 23/59 (38%), Gaps = 2/59 (3%)
Frame = -1
Query: 394 GRGGGGGERXXXGGGGXPPPXGXGGXXFXR-GGGGEXGXAXGRRXG-XGXXRXKXPXGF 224
GRGG GG R GG G GG GGG G G R G G R GF
Sbjct: 136 GRGGRGGFRGGRGGSRG----GFGGNSRGGFGGGSRGGFGGGSRGGSRGGFRGGSRGGF 190
Score = 27.5 bits (58), Expect = 3.5
Identities = 17/36 (47%), Positives = 17/36 (47%), Gaps = 3/36 (8%)
Frame = -3
Query: 701 GGAXRAGGXGX-GGRGXXXXXXGGX--GGLGGGXXG 603
G A R G G GGRG GG GG GGG G
Sbjct: 133 GPAGRGGRGGFRGGRGGSRGGFGGNSRGGFGGGSRG 168
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.5 bits (58), Expect = 3.5
Identities = 11/22 (50%), Positives = 11/22 (50%)
Frame = +1
Query: 328 PPXGGXXPPPPXXXSPPPPPPA 393
PP PPP S PPPP A
Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSA 1720
Score = 26.2 bits (55), Expect = 8.1
Identities = 12/26 (46%), Positives = 12/26 (46%), Gaps = 1/26 (3%)
Frame = +3
Query: 321 PPXPXG-GGXPPPPXXXLSPPPPPRP 395
PP P G PPPP S P P P
Sbjct: 1721 PPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 27.1 bits (57), Expect = 4.6
Identities = 10/26 (38%), Positives = 13/26 (50%)
Frame = +1
Query: 940 PXXARXPSSPLLXSXXPPXXXPPPPP 1017
P PS+P+ + P PPPPP
Sbjct: 1074 PSNISKPSAPVANNVSKPSAVPPPPP 1099
>SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 354
Score = 24.2 bits (50), Expect(2) = 4.9
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = -3
Query: 680 GXGXGGRGXXXXXXGGXGGLGG 615
G G GG G G GG GG
Sbjct: 225 GGGLGGAGLGGLGGAGLGGFGG 246
Score = 21.0 bits (42), Expect(2) = 4.9
Identities = 8/16 (50%), Positives = 8/16 (50%)
Frame = -3
Query: 635 GXGGLGGGXXGPPXPP 588
G GLG G G PP
Sbjct: 286 GDAGLGAGLGGAASPP 301
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.6 bits (56), Expect = 6.1
Identities = 13/32 (40%), Positives = 15/32 (46%), Gaps = 1/32 (3%)
Frame = +1
Query: 604 PXXPPPNP-PXPPXXXXXXPRPPXPXPPARXA 696
P PPP+P PP P P PPA+ A
Sbjct: 147 PSIPPPSPASAPPIPSKAPPIPSSLPPPAQPA 178
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,542,724
Number of Sequences: 5004
Number of extensions: 21868
Number of successful extensions: 435
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 58
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 280
length of database: 2,362,478
effective HSP length: 74
effective length of database: 1,992,182
effective search space used: 577732780
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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