BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_I13
(903 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 34 0.032
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 32 0.097
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 32 0.097
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.30
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 31 0.30
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 2.8
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 27 3.6
SPBC19C2.04c |ubp11||ubiquitin C-terminal hydrolase Ubp11|Schizo... 26 8.4
SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces pom... 26 8.4
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 33.9 bits (74), Expect = 0.032
Identities = 16/47 (34%), Positives = 16/47 (34%)
Frame = +3
Query: 531 PPXXXXPPPXXXPPXXXPPXXXXXPXARXPPPXGGGXXXPPPPXNXP 671
PP P P P PP PPP G PPPP P
Sbjct: 736 PPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 32.7 bits (71), Expect = 0.073
Identities = 17/50 (34%), Positives = 17/50 (34%)
Frame = +2
Query: 551 PPXPXPPXXXPPXXXXXPXGPXSPPXXGGVXXAPPPXKXXLXGXLGPPPP 700
PP P P P P P GG PPP G PPPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 31.5 bits (68), Expect = 0.17
Identities = 20/66 (30%), Positives = 22/66 (33%)
Frame = +2
Query: 527 PXPXXXXXPPXPXPPXXXPPXXXXXPXGPXSPPXXGGVXXAPPPXKXXLXGXLGPPPPXQ 706
P P P P P P GP PP GV A PP PPPP
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPP-------PPPPAV 785
Query: 707 XXXGNK 724
G++
Sbjct: 786 SAGGSR 791
Score = 27.9 bits (59), Expect = 2.1
Identities = 18/50 (36%), Positives = 18/50 (36%)
Frame = +3
Query: 549 PPPXXXPPXXXPPXXXXXPXARXPPPXGGGXXXPPPPXNXPXXGXWGPPP 698
PPP P P P A P GG PPPP G PPP
Sbjct: 735 PPPAVIVPTPAPAPIPVPPPA---PIMGGPPP-PPPPPGVAGAGPPPPPP 780
Score = 26.6 bits (56), Expect = 4.8
Identities = 11/23 (47%), Positives = 11/23 (47%)
Frame = -2
Query: 698 GGGAPXPXXGXVXGGGGPXXPPP 630
GG P P V G G P PPP
Sbjct: 759 GGPPPPPPPPGVAGAGPPPPPPP 781
Score = 25.8 bits (54), Expect = 8.4
Identities = 13/44 (29%), Positives = 14/44 (31%)
Frame = +2
Query: 527 PXPXXXXXPPXPXPPXXXPPXXXXXPXGPXSPPXXGGVXXAPPP 658
P P PP P PP P P + G AP P
Sbjct: 754 PAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAP 797
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 32.3 bits (70), Expect = 0.097
Identities = 19/56 (33%), Positives = 19/56 (33%)
Frame = -3
Query: 697 GGGPQXPXXGXFXGGGGXXNPPPXGGGXRAXGXXXXXGGXXXGGXXXGGGXXXXGG 530
G G G F GG G P G G G GG G GGG GG
Sbjct: 215 GEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGPGGHGG 270
Score = 31.5 bits (68), Expect = 0.17
Identities = 17/42 (40%), Positives = 17/42 (40%), Gaps = 1/42 (2%)
Frame = -3
Query: 670 GXFXG-GGGXXNPPPXGGGXRAXGXXXXXGGXXXGGXXXGGG 548
G F G GGG PPP GG G G G GGG
Sbjct: 188 GGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGG 229
Score = 30.3 bits (65), Expect = 0.39
Identities = 22/64 (34%), Positives = 22/64 (34%), Gaps = 5/64 (7%)
Frame = -2
Query: 707 FXXGGGAPXPXXGXVXGGGGPXXPPPXXGG----XXGPGXXXXXXGGXXXG-GXXXGGXG 543
F G G P P G G GG GG GPG GG G G GG G
Sbjct: 193 FGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLG 252
Query: 542 XXXG 531
G
Sbjct: 253 GFGG 256
Score = 28.7 bits (61), Expect = 1.2
Identities = 18/49 (36%), Positives = 18/49 (36%)
Frame = -2
Query: 671 GXVXGGGGPXXPPPXXGGXXGPGXXXXXXGGXXXGGXXXGGXGXXXGXG 525
G G GGP P GG G G GG GG G G G G
Sbjct: 192 GFGGGSGGPPPGPGGFGGFGGFGGEGHHHGG--HGGFGGGPGGFEGGPG 238
Score = 28.7 bits (61), Expect = 1.2
Identities = 31/97 (31%), Positives = 33/97 (34%), Gaps = 1/97 (1%)
Frame = -2
Query: 890 GGGAXXPPXXXGXXXGKRXXXXWGGXRGLWGGXXGGXRERXXXXGXXGVXKXXARFCXPX 711
GGG+ PP G G G G GG GG G G P
Sbjct: 194 GGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGP------GGFEG---------GPG 238
Query: 710 XFXXGGGAPXPXXGXVXG-GGGPXXPPPXXGGXXGPG 603
F GG P G + G GGGP GG GPG
Sbjct: 239 GF---GGGPGGFGGGLGGFGGGPGGFGGGPGGHGGPG 272
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 32.3 bits (70), Expect = 0.097
Identities = 15/44 (34%), Positives = 15/44 (34%)
Frame = +2
Query: 527 PXPXXXXXPPXPXPPXXXPPXXXXXPXGPXSPPXXGGVXXAPPP 658
P P PP P P PP P P GV PPP
Sbjct: 1151 PVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPP 1194
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 30.7 bits (66), Expect = 0.30
Identities = 13/36 (36%), Positives = 13/36 (36%)
Frame = +3
Query: 552 PPXXXPPXXXPPXXXXXPXARXPPPXGGGXXXPPPP 659
PP P PP P PP G PPPP
Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Score = 28.7 bits (61), Expect = 1.2
Identities = 13/41 (31%), Positives = 14/41 (34%)
Frame = +3
Query: 534 PXXXXPPPXXXPPXXXPPXXXXXPXARXPPPXGGGXXXPPP 656
P PP P PP P + P P G PPP
Sbjct: 1694 PQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Score = 27.1 bits (57), Expect = 3.6
Identities = 14/42 (33%), Positives = 14/42 (33%), Gaps = 1/42 (2%)
Frame = +3
Query: 534 PXXXXPPPXXXPPXXXPPXXXXXPXARXPP-PXGGGXXXPPP 656
P PP PP PP P A PP P P P
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 30.7 bits (66), Expect = 0.30
Identities = 14/42 (33%), Positives = 16/42 (38%)
Frame = +3
Query: 570 PXXXPPXXXXXPXARXPPPXGGGXXXPPPPXNXPXXGXWGPP 695
P PP + PP G PPPP + P G PP
Sbjct: 337 PPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPP 378
Score = 28.7 bits (61), Expect = 1.2
Identities = 25/96 (26%), Positives = 28/96 (29%)
Frame = +2
Query: 524 IPXPXXXXXPPXPXPPXXXPPXXXXXPXGPXSPPXXGGVXXAPPPXKXXLXGXLGPPPPX 703
IP P P P PP P P P S APPP + G P P
Sbjct: 351 IPLPPQGRSAPPPPPPRSAPSTGRQPP--PLSSSRAVSNPPAPPP---AIPGRSAPALPP 405
Query: 704 QXXXGNKNAPXFWXHXXXPXXXFSPXTPXXXPPTTP 811
+ P P P P PP+ P
Sbjct: 406 LGNASRTSTPPVPTPPSLPPSA-PPSLPPSAPPSLP 440
Score = 28.3 bits (60), Expect = 1.6
Identities = 14/46 (30%), Positives = 17/46 (36%), Gaps = 2/46 (4%)
Frame = +2
Query: 524 IPXPXXXXXPPXPXP--PXXXPPXXXXXPXGPXSPPXXGGVXXAPP 655
+P PP P P P P P +PP G+ APP
Sbjct: 423 LPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPP 468
Score = 26.6 bits (56), Expect = 4.8
Identities = 19/76 (25%), Positives = 22/76 (28%)
Frame = +3
Query: 552 PPXXXPPXXXPPXXXXXPXARXPPPXGGGXXXPPPPXNXPXXGXWGPPPXXKXGXXTKTR 731
PP PP R PPP PP P P G ++T
Sbjct: 354 PPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRT- 412
Query: 732 XXFGNTXGTPXXXSLP 779
+T P SLP
Sbjct: 413 ----STPPVPTPPSLP 424
Score = 26.6 bits (56), Expect = 4.8
Identities = 20/64 (31%), Positives = 21/64 (32%), Gaps = 6/64 (9%)
Frame = +2
Query: 527 PXPXXXXXPPXPXPPXXXPPXXXXXPXG-PXSPPXXGGVXXAPP-----PXKXXLXGXLG 688
P P PP PP P P G P +PP APP P L
Sbjct: 416 PVPTPPSLPP-SAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAP 474
Query: 689 PPPP 700
PPP
Sbjct: 475 APPP 478
Score = 26.6 bits (56), Expect = 4.8
Identities = 12/36 (33%), Positives = 12/36 (33%)
Frame = +2
Query: 551 PPXPXPPXXXPPXXXXXPXGPXSPPXXGGVXXAPPP 658
PP P PP P P PP AP P
Sbjct: 447 PPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAP 482
Score = 25.8 bits (54), Expect = 8.4
Identities = 12/40 (30%), Positives = 14/40 (35%)
Frame = +3
Query: 552 PPXXXPPXXXPPXXXXXPXARXPPPXGGGXXXPPPPXNXP 671
PP PP P P + P G PP P + P
Sbjct: 415 PPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAP 454
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 27.5 bits (58), Expect = 2.8
Identities = 20/85 (23%), Positives = 25/85 (29%)
Frame = +3
Query: 525 SXPPXXXXPPPXXXPPXXXPPXXXXXPXARXPPPXGGGXXXPPPPXNXPXXGXWGPPPXX 704
S PP PPP P P + PP PP + P PP
Sbjct: 142 SAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPP--SAPSLPSAVPPMPP 199
Query: 705 KXGXXTKTRXXFGNTXGTPXXXSLP 779
K ++ NT P + P
Sbjct: 200 KVPPPPLSQAPVANTSSRPSSFAPP 224
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 27.1 bits (57), Expect = 3.6
Identities = 16/47 (34%), Positives = 16/47 (34%)
Frame = +3
Query: 519 T*SXPPXXXXPPPXXXPPXXXPPXXXXXPXARXPPPXGGGXXXPPPP 659
T S P PPP PP P PPP PPPP
Sbjct: 157 TVSAPNSMVSPPPSFQPPSAAAPATSLPSDYNPPPP------PPPPP 197
>SPBC19C2.04c |ubp11||ubiquitin C-terminal hydrolase
Ubp11|Schizosaccharomyces pombe|chr 2|||Manual
Length = 350
Score = 25.8 bits (54), Expect = 8.4
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +3
Query: 12 LPPHYSGILKISTFSIVAHQRDQY 83
LPP YSG +S S++ R+Q+
Sbjct: 194 LPPEYSGNSNVSLLSLMEADREQH 217
>SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1562
Score = 25.8 bits (54), Expect = 8.4
Identities = 15/48 (31%), Positives = 32/48 (66%)
Frame = +2
Query: 98 LNVRALNLHKLTMNFAKILSFVFALVLALSMTSAAPEPRWKIFKKIEK 241
++V+A ++ K+T +I+ F+FALV+++++ A W+ KK+E+
Sbjct: 561 VSVKAPSMQKVTN---RIVIFIFALVVSMAIYCTAAYFVWQ--KKVER 603
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,737,029
Number of Sequences: 5004
Number of extensions: 48131
Number of successful extensions: 273
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 101
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 456499320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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