BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_I07
(914 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 37 0.005
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 36 0.006
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 29 0.043
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 31 0.17
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 31 0.30
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 30 0.40
SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 29 0.70
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 29 1.2
SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces... 27 2.8
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 27 3.7
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 27 3.7
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 27 3.7
SPBC660.05 |||conserved fungal protein|Schizosaccharomyces pombe... 26 6.5
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 6.5
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 36.7 bits (81), Expect = 0.005
Identities = 13/21 (61%), Positives = 13/21 (61%)
Frame = +3
Query: 705 PPPPPPPPXXXXXXFPXPPPP 767
PPPPPPPP P PPPP
Sbjct: 9 PPPPPPPPGFEPPSQPPPPPP 29
Score = 33.9 bits (74), Expect = 0.032
Identities = 12/22 (54%), Positives = 12/22 (54%)
Frame = +2
Query: 707 PPPPPPPXXXXXXFSXSPPPPP 772
PPPPPPP PPPPP
Sbjct: 9 PPPPPPPPGFEPPSQPPPPPPP 30
Score = 32.7 bits (71), Expect = 0.075
Identities = 12/21 (57%), Positives = 12/21 (57%)
Frame = +3
Query: 705 PPPPPPPPXXXXXXFPXPPPP 767
PPPPPPP P PPPP
Sbjct: 10 PPPPPPPGFEPPSQPPPPPPP 30
Score = 29.5 bits (63), Expect = 0.70
Identities = 13/25 (52%), Positives = 13/25 (52%), Gaps = 1/25 (4%)
Frame = +2
Query: 701 LPPP-PPPPPXXXXXXFSXSPPPPP 772
LPP PPPPP PPPPP
Sbjct: 4 LPPGNPPPPPPPPGFEPPSQPPPPP 28
Score = 26.6 bits (56), Expect = 4.9
Identities = 16/39 (41%), Positives = 17/39 (43%)
Frame = +3
Query: 705 PPPPPPPPXXXXXXFPXPPPPXLFXLFFXXGGRXPPPPP 821
PP PPPP PPPP F + PPPPP
Sbjct: 5 PPGNPPPP---------PPPPG-----FEPPSQPPPPPP 29
Score = 26.2 bits (55), Expect = 6.5
Identities = 9/13 (69%), Positives = 9/13 (69%)
Frame = +1
Query: 688 FXXXSXPPPPPPP 726
F S PPPPPPP
Sbjct: 18 FEPPSQPPPPPPP 30
Score = 25.8 bits (54), Expect = 8.6
Identities = 10/20 (50%), Positives = 10/20 (50%)
Frame = +2
Query: 758 PPPPPFXXFFXXGGPXXPPP 817
PPPPP F P PPP
Sbjct: 10 PPPPPPPGFEPPSQPPPPPP 29
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 36.3 bits (80), Expect = 0.006
Identities = 13/21 (61%), Positives = 13/21 (61%)
Frame = +3
Query: 705 PPPPPPPPXXXXXXFPXPPPP 767
PPPPPPPP P PPPP
Sbjct: 761 PPPPPPPPGVAGAGPPPPPPP 781
Score = 35.1 bits (77), Expect = 0.014
Identities = 18/42 (42%), Positives = 19/42 (45%), Gaps = 3/42 (7%)
Frame = +3
Query: 705 PPPPPPP---PXXXXXXFPXPPPPXLFXLFFXXGGRXPPPPP 821
PPPPPP P P PPP + GG PPPPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIM------GGPPPPPPP 767
Score = 32.3 bits (70), Expect = 0.099
Identities = 14/37 (37%), Positives = 14/37 (37%)
Frame = +2
Query: 707 PPPPPPPXXXXXXFSXSPPPPPFXXFFXXGGPXXPPP 817
P P P P PPPPP GP PPP
Sbjct: 744 PAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780
Score = 31.9 bits (69), Expect = 0.13
Identities = 17/40 (42%), Positives = 19/40 (47%), Gaps = 1/40 (2%)
Frame = +2
Query: 701 LPPPPPPPPXXXXXXFSXSP-PPPPFXXFFXXGGPXXPPP 817
L PPPPPP + +P P PP GGP PPP
Sbjct: 729 LKSPPPPPPAVIVPTPAPAPIPVPPPAPIM--GGPPPPPP 766
Score = 31.1 bits (67), Expect = 0.23
Identities = 14/38 (36%), Positives = 14/38 (36%)
Frame = +2
Query: 704 PPPPPPPPXXXXXXFSXSPPPPPFXXFFXXGGPXXPPP 817
P P P P PPPPP G P PPP
Sbjct: 744 PAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 29.5 bits (63), Expect = 0.70
Identities = 14/37 (37%), Positives = 14/37 (37%)
Frame = +3
Query: 711 PPPPPPXXXXXXFPXPPPPXLFXLFFXXGGRXPPPPP 821
PPPPPP PPPP G R P P
Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAP 797
Score = 27.1 bits (57), Expect = 3.7
Identities = 11/23 (47%), Positives = 11/23 (47%)
Frame = +2
Query: 704 PPPPPPPPXXXXXXFSXSPPPPP 772
PPPPPPPP S P P
Sbjct: 775 PPPPPPPPPAVSAGGSRYYAPAP 797
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 29.5 bits (63), Expect = 0.70
Identities = 13/43 (30%), Positives = 17/43 (39%)
Frame = +2
Query: 704 PPPPPPPPXXXXXXFSXSPPPPPFXXFFXXGGPXXPPPXXXKK 832
PPPPPPPP + + P + P PP K+
Sbjct: 189 PPPPPPPPPAVEDQAADANEPDDYYSSGRAVSPEIPPTYTPKQ 231
Score = 27.5 bits (58), Expect = 2.8
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = +3
Query: 699 FXPPPPPPPP 728
+ PPPPPPPP
Sbjct: 187 YNPPPPPPPP 196
Score = 27.1 bits (57), Expect(2) = 0.043
Identities = 13/37 (35%), Positives = 14/37 (37%)
Frame = +2
Query: 707 PPPPPPPXXXXXXFSXSPPPPPFXXFFXXGGPXXPPP 817
PPPPPPP + S P P G PP
Sbjct: 238 PPPPPPPTLPPQSTNTSQLPMPSRNVNNLGSQVNIPP 274
Score = 25.0 bits (52), Expect(2) = 0.043
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = +2
Query: 701 LPPPPPPPP 727
LP PPPPPP
Sbjct: 235 LPAPPPPPP 243
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 31.5 bits (68), Expect = 0.17
Identities = 17/42 (40%), Positives = 17/42 (40%), Gaps = 5/42 (11%)
Frame = +3
Query: 705 PPPPPPPPXXXXXXFPXPP-----PPXLFXLFFXXGGRXPPP 815
PPPPPPP P PP PP GR PPP
Sbjct: 337 PPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPP 378
Score = 29.1 bits (62), Expect = 0.92
Identities = 17/44 (38%), Positives = 17/44 (38%), Gaps = 7/44 (15%)
Frame = +2
Query: 704 PPPPPP-------PPXXXXXXFSXSPPPPPFXXFFXXGGPXXPP 814
PPPPPP PP S PPPPP G PP
Sbjct: 312 PPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPP 355
Score = 28.7 bits (61), Expect = 1.2
Identities = 14/36 (38%), Positives = 14/36 (38%)
Frame = +2
Query: 710 PPPPPPXXXXXXFSXSPPPPPFXXFFXXGGPXXPPP 817
PPPPPP S PPP P PPP
Sbjct: 361 PPPPPPRSAP---STGRQPPPLSSSRAVSNPPAPPP 393
Score = 26.6 bits (56), Expect = 4.9
Identities = 10/21 (47%), Positives = 10/21 (47%)
Frame = +1
Query: 700 SXPPPPPPPPXXXXXFFXFPP 762
S PPPPPPP PP
Sbjct: 335 SLPPPPPPPRSNAAGSIPLPP 355
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 30.7 bits (66), Expect = 0.30
Identities = 17/43 (39%), Positives = 17/43 (39%)
Frame = -2
Query: 832 FFXXGGGGGXRPPXXKKXXKRXGGGGXGKXXXXXXGGGGGGGG 704
F GGG G PP G GG G G GGG GG
Sbjct: 190 FGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGG 232
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 30.3 bits (65), Expect = 0.40
Identities = 16/37 (43%), Positives = 16/37 (43%)
Frame = +2
Query: 707 PPPPPPPXXXXXXFSXSPPPPPFXXFFXXGGPXXPPP 817
P PPPPP S PPPP G P PPP
Sbjct: 1705 PTPPPPPM--------SVPPPPSAPPMPAGPPSAPPP 1733
>SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein
Cap1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 551
Score = 29.5 bits (63), Expect = 0.70
Identities = 11/23 (47%), Positives = 12/23 (52%)
Frame = +2
Query: 701 LPPPPPPPPXXXXXXFSXSPPPP 769
LPPPPPPPP + P P
Sbjct: 305 LPPPPPPPPPSNDFWKDSNEPAP 327
Score = 27.1 bits (57), Expect = 3.7
Identities = 10/22 (45%), Positives = 11/22 (50%)
Frame = +2
Query: 707 PPPPPPPXXXXXXFSXSPPPPP 772
PPPPPPP + S P P
Sbjct: 306 PPPPPPPPPSNDFWKDSNEPAP 327
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 28.7 bits (61), Expect = 1.2
Identities = 9/10 (90%), Positives = 9/10 (90%)
Frame = +3
Query: 699 FXPPPPPPPP 728
F PPPPPPPP
Sbjct: 944 FPPPPPPPPP 953
Score = 26.6 bits (56), Expect = 4.9
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +1
Query: 706 PPPPPPPP 729
PPPPPPPP
Sbjct: 945 PPPPPPPP 952
Score = 26.6 bits (56), Expect = 4.9
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +1
Query: 706 PPPPPPPP 729
PPPPPPPP
Sbjct: 946 PPPPPPPP 953
>SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 713
Score = 27.5 bits (58), Expect = 2.8
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = +3
Query: 699 FXPPPPPPPP 728
+ PPPPPPPP
Sbjct: 368 YSPPPPPPPP 377
Score = 26.6 bits (56), Expect = 4.9
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +1
Query: 706 PPPPPPPP 729
PPPPPPPP
Sbjct: 370 PPPPPPPP 377
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 27.1 bits (57), Expect = 3.7
Identities = 17/47 (36%), Positives = 18/47 (38%), Gaps = 9/47 (19%)
Frame = +3
Query: 705 PPPP---------PPPPXXXXXXFPXPPPPXLFXLFFXXGGRXPPPP 818
PPPP PPPP PPPP + G PPPP
Sbjct: 221 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPP----MHHEPGEHMPPPP 263
Score = 27.1 bits (57), Expect = 3.7
Identities = 17/47 (36%), Positives = 18/47 (38%), Gaps = 9/47 (19%)
Frame = +3
Query: 705 PPPP---------PPPPXXXXXXFPXPPPPXLFXLFFXXGGRXPPPP 818
PPPP PPPP PPPP + G PPPP
Sbjct: 247 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPP----MHHEPGEHMPPPP 289
Score = 27.1 bits (57), Expect = 3.7
Identities = 17/47 (36%), Positives = 18/47 (38%), Gaps = 9/47 (19%)
Frame = +3
Query: 705 PPPP---------PPPPXXXXXXFPXPPPPXLFXLFFXXGGRXPPPP 818
PPPP PPPP PPPP + G PPPP
Sbjct: 273 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPP----MHHEPGEHMPPPP 315
Score = 27.1 bits (57), Expect = 3.7
Identities = 17/47 (36%), Positives = 18/47 (38%), Gaps = 9/47 (19%)
Frame = +3
Query: 705 PPPP---------PPPPXXXXXXFPXPPPPXLFXLFFXXGGRXPPPP 818
PPPP PPPP PPPP + G PPPP
Sbjct: 299 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPP----MHHEPGEHMPPPP 341
Score = 26.6 bits (56), Expect = 4.9
Identities = 13/34 (38%), Positives = 14/34 (41%)
Frame = +3
Query: 717 PPPPXXXXXXFPXPPPPXLFXLFFXXGGRXPPPP 818
PPPP PPPP + G PPPP
Sbjct: 208 PPPPMHHKPGEHMPPPP----MHHEPGEHMPPPP 237
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 27.1 bits (57), Expect = 3.7
Identities = 11/23 (47%), Positives = 11/23 (47%)
Frame = +2
Query: 701 LPPPPPPPPXXXXXXFSXSPPPP 769
LPPPPP SPPPP
Sbjct: 907 LPPPPPTASMTASAPAIASPPPP 929
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 27.1 bits (57), Expect = 3.7
Identities = 8/9 (88%), Positives = 9/9 (100%)
Frame = +2
Query: 701 LPPPPPPPP 727
+PPPPPPPP
Sbjct: 1094 VPPPPPPPP 1102
Score = 26.6 bits (56), Expect = 4.9
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +1
Query: 706 PPPPPPPP 729
PPPPPPPP
Sbjct: 1095 PPPPPPPP 1102
>SPBC660.05 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 143
Score = 26.2 bits (55), Expect = 6.5
Identities = 13/32 (40%), Positives = 13/32 (40%)
Frame = -2
Query: 799 PPXXKKXXKRXGGGGXGKXXXXXXGGGGGGGG 704
PP R GGG G G GG GGG
Sbjct: 101 PPRGPLFGPRIGGGHHGPLHGPHGGFGGRGGG 132
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.2 bits (55), Expect = 6.5
Identities = 9/20 (45%), Positives = 11/20 (55%)
Frame = +2
Query: 704 PPPPPPPPXXXXXXFSXSPP 763
P PPPPPP + +PP
Sbjct: 3 PAPPPPPPAPAPAAAAPAPP 22
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,157,955
Number of Sequences: 5004
Number of extensions: 36065
Number of successful extensions: 472
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 50
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 163
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 464508080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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