BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP03_F_H10 (1102 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 31 0.12 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 25 0.27 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 31 0.29 SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 28 2.0 SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 23 5.5 SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M... 26 8.1 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 30.7 bits (66), Expect = 0.38 Identities = 11/20 (55%), Positives = 11/20 (55%) Frame = +2 Query: 761 PPPXXGXGGXXVXXPPPPPP 820 PPP G G PPPPPP Sbjct: 764 PPPPPGVAGAGPPPPPPPPP 783 Score = 29.9 bits (64), Expect = 0.66 Identities = 11/20 (55%), Positives = 11/20 (55%) Frame = +3 Query: 759 PPPPXXGXGGXXSFXPPPPP 818 PPPP G G PPPPP Sbjct: 763 PPPPPPGVAGAGPPPPPPPP 782 Score = 29.1 bits (62), Expect = 1.2 Identities = 12/24 (50%), Positives = 12/24 (50%) Frame = +1 Query: 748 GXGXPPPXXGGGGGXGXFXPPPPP 819 G PPP G G G PPPPP Sbjct: 759 GGPPPPPPPPGVAGAGPPPPPPPP 782 Score = 29.1 bits (62), Expect(2) = 0.12 Identities = 11/21 (52%), Positives = 11/21 (52%) Frame = +2 Query: 911 PXPPPPPKXPXXXFLPPPXPP 973 P PPPPP PPP PP Sbjct: 761 PPPPPPPPGVAGAGPPPPPPP 781 Score = 28.7 bits (61), Expect = 1.5 Identities = 11/21 (52%), Positives = 11/21 (52%) Frame = +2 Query: 911 PXPPPPPKXPXXXFLPPPXPP 973 P PPPPP PPP PP Sbjct: 762 PPPPPPPGVAGAGPPPPPPPP 782 Score = 27.9 bits (59), Expect = 2.7 Identities = 11/23 (47%), Positives = 11/23 (47%) Frame = +3 Query: 750 PGXPPPPXXGXGGXXSFXPPPPP 818 P PPPP G PPPPP Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPPP 783 Score = 27.1 bits (57), Expect = 4.6 Identities = 10/20 (50%), Positives = 10/20 (50%) Frame = -3 Query: 806 GXKXPXPPPPPXXGGGXPXP 747 G P PPPP G G P P Sbjct: 759 GGPPPPPPPPGVAGAGPPPP 778 Score = 26.2 bits (55), Expect = 8.1 Identities = 11/25 (44%), Positives = 11/25 (44%) Frame = +2 Query: 911 PXPPPPPKXPXXXFLPPPXPPXXGG 985 P P PPP PPP PP G Sbjct: 748 PIPVPPPAPIMGGPPPPPPPPGVAG 772 Score = 21.8 bits (44), Expect(2) = 0.12 Identities = 10/19 (52%), Positives = 10/19 (52%) Frame = +2 Query: 761 PPPXXGXGGXXVXXPPPPP 817 PPP GG PPPPP Sbjct: 752 PPPAPIMGGPPP--PPPPP 768 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 25.0 bits (52), Expect(2) = 0.27 Identities = 9/18 (50%), Positives = 9/18 (50%) Frame = +2 Query: 767 PXXGXGGXXVXXPPPPPP 820 P G G PPPPPP Sbjct: 325 PPIGNGSSNSSLPPPPPP 342 Score = 24.6 bits (51), Expect(2) = 0.46 Identities = 9/21 (42%), Positives = 10/21 (47%) Frame = +2 Query: 755 GXPPPXXGXGGXXVXXPPPPP 817 G PP G + PPPPP Sbjct: 323 GKPPIGNGSSNSSLPPPPPPP 343 Score = 24.6 bits (51), Expect(2) = 0.27 Identities = 9/18 (50%), Positives = 9/18 (50%) Frame = +2 Query: 911 PXPPPPPKXPXXXFLPPP 964 P PPPP P PPP Sbjct: 361 PPPPPPRSAPSTGRQPPP 378 Score = 24.2 bits (50), Expect(2) = 0.46 Identities = 12/30 (40%), Positives = 13/30 (43%), Gaps = 9/30 (30%) Frame = +2 Query: 911 PXPPPPPK---------XPXXXFLPPPXPP 973 P PPPPP+ P PPP PP Sbjct: 337 PPPPPPPRSNAAGSIPLPPQGRSAPPPPPP 366 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 31.1 bits (67), Expect = 0.29 Identities = 12/23 (52%), Positives = 12/23 (52%) Frame = +3 Query: 750 PGXPPPPXXGXGGXXSFXPPPPP 818 PG PPPP G PPPPP Sbjct: 6 PGNPPPPPPPPGFEPPSQPPPPP 28 Score = 29.1 bits (62), Expect = 1.2 Identities = 11/21 (52%), Positives = 11/21 (52%) Frame = +2 Query: 911 PXPPPPPKXPXXXFLPPPXPP 973 P PPPPP PPP PP Sbjct: 9 PPPPPPPPGFEPPSQPPPPPP 29 Score = 29.1 bits (62), Expect = 1.2 Identities = 11/21 (52%), Positives = 11/21 (52%) Frame = +2 Query: 911 PXPPPPPKXPXXXFLPPPXPP 973 P PPPPP PPP PP Sbjct: 10 PPPPPPPGFEPPSQPPPPPPP 30 Score = 26.2 bits (55), Expect = 8.1 Identities = 10/20 (50%), Positives = 10/20 (50%) Frame = +2 Query: 761 PPPXXGXGGXXVXXPPPPPP 820 PPP G PPPPPP Sbjct: 10 PPPPPPPGFEPPSQPPPPPP 29 Score = 26.2 bits (55), Expect = 8.1 Identities = 10/20 (50%), Positives = 10/20 (50%) Frame = +3 Query: 759 PPPPXXGXGGXXSFXPPPPP 818 PPPP G PPPPP Sbjct: 11 PPPPPPGFEPPSQPPPPPPP 30 >SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 273 Score = 28.3 bits (60), Expect = 2.0 Identities = 11/18 (61%), Positives = 11/18 (61%) Frame = +1 Query: 748 GXGXPPPXXGGGGGXGXF 801 G G PPP GG GG G F Sbjct: 196 GSGGPPPGPGGFGGFGGF 213 >SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family |Schizosaccharomyces pombe|chr 1|||Manual Length = 1420 Score = 22.6 bits (46), Expect(2) = 5.5 Identities = 7/10 (70%), Positives = 7/10 (70%) Frame = +2 Query: 911 PXPPPPPKXP 940 P PPPPP P Sbjct: 238 PPPPPPPTLP 247 Score = 22.2 bits (45), Expect(2) = 5.5 Identities = 8/20 (40%), Positives = 9/20 (45%) Frame = +2 Query: 761 PPPXXGXGGXXVXXPPPPPP 820 PP + PPPPPP Sbjct: 224 PPTYTPKQADPLPAPPPPPP 243 >SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||Manual Length = 500 Score = 26.2 bits (55), Expect = 8.1 Identities = 10/20 (50%), Positives = 12/20 (60%) Frame = -2 Query: 963 GGGRKXXXGXFGGGGGXGXK 904 GGG + G FGG GG G + Sbjct: 446 GGGSRGGRGGFGGRGGFGGR 465 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,451,287 Number of Sequences: 5004 Number of extensions: 36947 Number of successful extensions: 255 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 42 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 178 length of database: 2,362,478 effective HSP length: 74 effective length of database: 1,992,182 effective search space used: 581717144 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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