Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_G20
(850 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g17483.1 68417.m02615 palmitoyl protein thioesterase family p... 48 8e-06
At3g60340.2 68416.m06746 palmitoyl protein thioesterase family p... 44 1e-04
At3g60340.1 68416.m06745 palmitoyl protein thioesterase family p... 44 1e-04
At5g47340.1 68418.m05835 palmitoyl protein thioesterase family p... 42 5e-04
At4g17470.1 68417.m02613 palmitoyl protein thioesterase family p... 41 0.001
At5g47330.1 68418.m05834 palmitoyl protein thioesterase family p... 40 0.002
At4g17480.1 68417.m02614 palmitoyl protein thioesterase family p... 40 0.003
At3g46810.1 68416.m05081 DC1 domain-containing protein contains ... 31 0.73
At3g11370.1 68416.m01382 DC1 domain-containing protein contains ... 31 0.97
At5g47350.1 68418.m05836 palmitoyl protein thioesterase family p... 30 1.7
At3g46800.1 68416.m05080 DC1 domain-containing protein contains ... 30 2.2
At4g28470.1 68417.m04073 26S proteasome regulatory subunit, puta... 29 3.0
At1g11380.1 68414.m01307 expressed protein contains Pfam profile... 29 3.0
At2g02620.1 68415.m00201 DC1 domain-containing protein / PHD fin... 29 3.9
At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329... 28 6.8
At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329... 28 6.8
At2g20380.1 68415.m02379 kelch repeat-containing F-box family pr... 28 6.8
>At4g17483.1 68417.m02615 palmitoyl protein thioesterase family
protein
Length = 300
Score = 48.0 bits (109), Expect = 8e-06
Identities = 30/84 (35%), Positives = 44/84 (52%)
Frame = +1
Query: 202 PIVLWHGMGDTCCMSFSLGSFKIFLEKNIPGVYVLSLQIGNNTVEDFENGYFMNPNLQVE 381
P +L+HG+GD C S + +F L N+ G L+IGN ++ +FM Q
Sbjct: 30 PFILFHGIGDKC--SGGVSNFTQLLT-NLSGSPGYCLEIGNGE----KDSWFMPLRKQTS 82
Query: 382 YVCEKLAADPKLSNGFNVMGFSQG 453
CEK+ A LS G+N++ SQG
Sbjct: 83 VACEKVKAMKVLSQGYNIVAESQG 106
>At3g60340.2 68416.m06746 palmitoyl protein thioesterase family
protein palmitoyl-protein thioesterase precursor, Mus
musculus, EMBL:AF071025
Length = 338
Score = 44.0 bits (99), Expect = 1e-04
Identities = 29/89 (32%), Positives = 48/89 (53%), Gaps = 1/89 (1%)
Frame = +1
Query: 190 ATPTPIVLWHGMGDTCCMSFSLGSFKIFLEKNIPGVYVLSLQIGNNTVEDFENGYFMNPN 369
A+ P ++ HG+GD C + + F L + G L+IGN + + + P
Sbjct: 20 ASSVPFIVLHGIGDKCSNA-GVTQFTELLS-DWSGSQGYCLEIGNGSWDSWTM-----PL 72
Query: 370 L-QVEYVCEKLAADPKLSNGFNVMGFSQG 453
L Q VCEK+ + P+LS+G++++G SQG
Sbjct: 73 LDQTSVVCEKVKSMPELSDGYSIVGLSQG 101
>At3g60340.1 68416.m06745 palmitoyl protein thioesterase family
protein palmitoyl-protein thioesterase precursor, Mus
musculus, EMBL:AF071025
Length = 338
Score = 44.0 bits (99), Expect = 1e-04
Identities = 29/89 (32%), Positives = 48/89 (53%), Gaps = 1/89 (1%)
Frame = +1
Query: 190 ATPTPIVLWHGMGDTCCMSFSLGSFKIFLEKNIPGVYVLSLQIGNNTVEDFENGYFMNPN 369
A+ P ++ HG+GD C + + F L + G L+IGN + + + P
Sbjct: 20 ASSVPFIVLHGIGDKCSNA-GVTQFTELLS-DWSGSQGYCLEIGNGSWDSWTM-----PL 72
Query: 370 L-QVEYVCEKLAADPKLSNGFNVMGFSQG 453
L Q VCEK+ + P+LS+G++++G SQG
Sbjct: 73 LDQTSVVCEKVKSMPELSDGYSIVGLSQG 101
>At5g47340.1 68418.m05835 palmitoyl protein thioesterase family
protein
Length = 317
Score = 41.9 bits (94), Expect = 5e-04
Identities = 24/84 (28%), Positives = 39/84 (46%)
Frame = +1
Query: 202 PIVLWHGMGDTCCMSFSLGSFKIFLEKNIPGVYVLSLQIGNNTVEDFENGYFMNPNLQVE 381
P ++ HG+ C + ++ N+ G L+IGN + N F+ Q E
Sbjct: 27 PFIMLHGIASQCSDDTNANFTQLLT--NLSGSPGFCLEIGNGVI----NSMFLPLTQQAE 80
Query: 382 YVCEKLAADPKLSNGFNVMGFSQG 453
CE + +LS G+N++G SQG
Sbjct: 81 IACENVKEMKELSQGYNIVGRSQG 104
>At4g17470.1 68417.m02613 palmitoyl protein thioesterase family
protein
Length = 308
Score = 40.7 bits (91), Expect = 0.001
Identities = 27/84 (32%), Positives = 40/84 (47%)
Frame = +1
Query: 202 PIVLWHGMGDTCCMSFSLGSFKIFLEKNIPGVYVLSLQIGNNTVEDFENGYFMNPNLQVE 381
P VL+HG G C + + FL N G ++IG+ ++ FM Q
Sbjct: 28 PFVLFHGFGGECSND-KVSNLTQFLI-NHSGYPGTCVEIGDGV----KDSLFMPLRQQAS 81
Query: 382 YVCEKLAADPKLSNGFNVMGFSQG 453
CEK+ P+LS G+N++ SQG
Sbjct: 82 IACEKIKQMPELSEGYNIVAESQG 105
>At5g47330.1 68418.m05834 palmitoyl protein thioesterase family
protein
Length = 314
Score = 40.3 bits (90), Expect = 0.002
Identities = 24/84 (28%), Positives = 39/84 (46%)
Frame = +1
Query: 202 PIVLWHGMGDTCCMSFSLGSFKIFLEKNIPGVYVLSLQIGNNTVEDFENGYFMNPNLQVE 381
P ++ HG+ C + ++ N+ G L+IGN + + M Q E
Sbjct: 28 PFIMLHGISAQCSNARDANFTQLLT--NLSGSPGFCLEIGNGVADS----WLMPLTRQAE 81
Query: 382 YVCEKLAADPKLSNGFNVMGFSQG 453
CEK+ +LS G+N++G SQG
Sbjct: 82 IACEKVKQMKELSQGYNIVGRSQG 105
>At4g17480.1 68417.m02614 palmitoyl protein thioesterase family
protein
Length = 304
Score = 39.5 bits (88), Expect = 0.003
Identities = 26/84 (30%), Positives = 40/84 (47%)
Frame = +1
Query: 202 PIVLWHGMGDTCCMSFSLGSFKIFLEKNIPGVYVLSLQIGNNTVEDFENGYFMNPNLQVE 381
P +L+HG+ D C + SF + L N+ + L+IGN ++ M Q
Sbjct: 27 PFILFHGIRDQCSNG-GVSSF-VQLLSNLSSSHGSCLEIGNGE----QDSVTMPLTQQAS 80
Query: 382 YVCEKLAADPKLSNGFNVMGFSQG 453
CEK+ LS G+N++ SQG
Sbjct: 81 VACEKVKQMKDLSQGYNIVAQSQG 104
>At3g46810.1 68416.m05081 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 686
Score = 31.5 bits (68), Expect = 0.73
Identities = 15/48 (31%), Positives = 25/48 (52%), Gaps = 2/48 (4%)
Frame = +3
Query: 492 INYH--KLKTWCLSEVSTKGIYGIPHCGALRHETCDDVRKLLNYAAYN 629
IN H KL C+S+++T Y CG + H+TC ++ + + N
Sbjct: 395 INLHESKLCEACVSQINTDPFYSCELCGFILHQTCANMPRKKRHVLCN 442
>At3g11370.1 68416.m01382 DC1 domain-containing protein contains
Pfam protein PF03107 DC1 domain
Length = 589
Score = 31.1 bits (67), Expect = 0.97
Identities = 13/48 (27%), Positives = 24/48 (50%)
Frame = +3
Query: 483 DVVINYHKLKTWCLSEVSTKGIYGIPHCGALRHETCDDVRKLLNYAAY 626
D V + KL C+ ++++ Y HC HE C + + L++A +
Sbjct: 315 DAVRDAEKLCQACVCPINSRDFYNCIHCDFFLHEVCAGLLRKLDHALH 362
>At5g47350.1 68418.m05836 palmitoyl protein thioesterase family
protein
Length = 316
Score = 30.3 bits (65), Expect = 1.7
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +1
Query: 373 QVEYVCEKLAADPKLSNGFNVMGFSQGMSV 462
Q E C+K+ +LS G+N++G +QG V
Sbjct: 80 QAEVACDKVTQMEELSQGYNIVGRAQGSLV 109
Score = 27.9 bits (59), Expect = 9.0
Identities = 9/20 (45%), Positives = 14/20 (70%)
Frame = +2
Query: 791 WVMFDNDXIXXPKETEWVRF 850
+V+F+ND + PKE+ W F
Sbjct: 218 FVLFENDTVIVPKESSWFGF 237
>At3g46800.1 68416.m05080 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 682
Score = 29.9 bits (64), Expect = 2.2
Identities = 10/37 (27%), Positives = 19/37 (51%)
Frame = +3
Query: 519 CLSEVSTKGIYGIPHCGALRHETCDDVRKLLNYAAYN 629
C S+++ YG C + HETC + + + + + N
Sbjct: 413 CASKINNDPFYGCEQCDFILHETCASLPRKIRHVSNN 449
>At4g28470.1 68417.m04073 26S proteasome regulatory subunit,
putative contains Pfam domain PF01851:
Proteasome/cyclosome repeat
Length = 1103
Score = 29.5 bits (63), Expect = 3.0
Identities = 17/44 (38%), Positives = 24/44 (54%)
Frame = -1
Query: 847 PDPFRFLRXXYXIIIEHHPARNLXRLFKFCIYVLLTVLTLLMSA 716
P P +FLR Y ++ E H A+ K + +L+VL L MSA
Sbjct: 87 PKPLKFLRPHYGVLKEFH-AKMAESDLKKMLADILSVLALTMSA 129
>At1g11380.1 68414.m01307 expressed protein contains Pfam profile
PF04749: Protein of unknown function, DUF614
Length = 254
Score = 29.5 bits (63), Expect = 3.0
Identities = 15/30 (50%), Positives = 19/30 (63%), Gaps = 1/30 (3%)
Frame = +1
Query: 211 LWHGMGDTCCM-SFSLGSFKIFLEKNIPGV 297
+WHG GDT C+ +S G K FLE + P V
Sbjct: 217 IWHGRGDTLCIGGYSEG--KAFLELHSPPV 244
>At2g02620.1 68415.m00201 DC1 domain-containing protein / PHD finger
protein-related contains Pfam profiles PF03107: DC1
domain, weak hit to PF00628: PHD-finger
Length = 513
Score = 29.1 bits (62), Expect = 3.9
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = +3
Query: 519 CLSEVSTKGIYGIPHCGALRHETCDD 596
C+ ++ K +Y CG + HETC D
Sbjct: 255 CVLPINEKNLYVCVECGFILHETCAD 280
>At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 442
Score = 28.3 bits (60), Expect = 6.8
Identities = 16/61 (26%), Positives = 33/61 (54%), Gaps = 1/61 (1%)
Frame = +1
Query: 391 EKLAADPKLSNGFNVMGFSQGMSVYSSCST-KMWS*TTTN*KHGVSRRSAPRASMAYLIV 567
+K+ P++SN N++GF + + CS K W+ T +S+RS ++S ++ +
Sbjct: 72 KKIEYKPEISNWINLIGFVEQPVQFGPCSDGKFWAGTV------ISQRSGSKSSNFWIPI 125
Query: 568 V 570
+
Sbjct: 126 I 126
>At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 440
Score = 28.3 bits (60), Expect = 6.8
Identities = 16/61 (26%), Positives = 33/61 (54%), Gaps = 1/61 (1%)
Frame = +1
Query: 391 EKLAADPKLSNGFNVMGFSQGMSVYSSCST-KMWS*TTTN*KHGVSRRSAPRASMAYLIV 567
+K+ P++SN N++GF + + CS K W+ T +S+RS ++S ++ +
Sbjct: 72 KKIEYKPEISNWINLIGFVEQPVQFGPCSDGKFWAGTV------ISQRSGSKSSNFWIPI 125
Query: 568 V 570
+
Sbjct: 126 I 126
>At2g20380.1 68415.m02379 kelch repeat-containing F-box family
protein contains F-box domain Pfam:PF00646 and Kelch
motif Pfam:PF01344
Length = 348
Score = 28.3 bits (60), Expect = 6.8
Identities = 18/62 (29%), Positives = 34/62 (54%), Gaps = 1/62 (1%)
Frame = +1
Query: 265 KIFLEKNIPGV-YVLSLQIGNNTVEDFENGYFMNPNLQVEYVCEKLAADPKLSNGFNVMG 441
+IF +++ G+ Y+ ++ + +D E + P L+ E +A PK+S G+ V+G
Sbjct: 164 RIFKLQHMKGMLYMKVSKLLSFVAQDAEELAPLFPKLRSLLSTEVVAFKPKVSEGYEVLG 223
Query: 442 FS 447
FS
Sbjct: 224 FS 225
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,716,222
Number of Sequences: 28952
Number of extensions: 361241
Number of successful extensions: 875
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 829
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 870
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1970388800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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