BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP03_F_G12 (1245 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 33 0.062 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 33 0.082 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 31 0.33 SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 31 0.44 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 29 1.0 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 7.2 SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex p... 27 7.2 SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 26 9.5 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 33.5 bits (73), Expect = 0.062 Identities = 23/70 (32%), Positives = 25/70 (35%), Gaps = 6/70 (8%) Frame = +3 Query: 435 PXSXPPPXRXL---VXXPP--RXXPXPPPP-SXPSPXXXXPPXXXXXXLXXXXFXHPPAP 596 P PPP + PP R P PPPP S PS PP + P P Sbjct: 337 PPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIP 396 Query: 597 GGGXXXXPPL 626 G PPL Sbjct: 397 GRSAPALPPL 406 Score = 31.1 bits (67), Expect = 0.33 Identities = 16/55 (29%), Positives = 19/55 (34%) Frame = +2 Query: 1055 PPPPQNXTXXXXXXPPSPXXSXSPPXXXXPKXQKXXXPPPAXAXXXKXPXPLPPP 1219 PPPP++ PP S PP PPP + P PPP Sbjct: 340 PPPPRSNAAGSIPLPPQ-GRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPP 393 Score = 27.1 bits (57), Expect = 5.4 Identities = 36/177 (20%), Positives = 42/177 (23%), Gaps = 5/177 (2%) Frame = +1 Query: 112 PPPPXPXXXLXXPXGKFFXPTPNXLXL*XPXXXSPPPXGGXGKKXKXXGXXXLXPPXXPX 291 PPPP P + N P PP G PP P Sbjct: 311 PPPPPPPSRRNRGKPPIGNGSSNSSL---PPPPPPPRSNAAGSIPLPPQGRSAPPPPPPR 367 Query: 292 XXPPFSPPPXXXXHKQKXXXPPXTKKNKXXPCXXSSXXQKXXGXGXXTPSXXXPLXVLSX 471 P P + PP P G + + P+ Sbjct: 368 SAPSTGRQPPPLSSSRAVSNPPAPP-----PAIPGRSAPALPPLGNASRTSTPPVPTPPS 422 Query: 472 XPPLGXXPXPP--PPXXP---PPXXXLPPXXPXXXXXXXXFXTRPPPGGGXNXXPPS 627 PP PP PP P P LPP P PP PP+ Sbjct: 423 LPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPA 479 Score = 26.2 bits (55), Expect = 9.5 Identities = 11/30 (36%), Positives = 12/30 (40%) Frame = +3 Query: 435 PXSXPPPXRXLVXXPPRXXPXPPPPSXPSP 524 P PPP V PP P P P +P Sbjct: 253 PPPIPPPSNGTVSSPPNSPPRPIAPVSMNP 282 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 33.1 bits (72), Expect = 0.082 Identities = 19/55 (34%), Positives = 19/55 (34%) Frame = +3 Query: 435 PXSXPPPXRXLVXXPPRXXPXPPPPSXPSPXXXXPPXXXXXXLXXXXFXHPPAPG 599 P S PP PP PPPPS P P PP L P PG Sbjct: 1694 PQSAAPPQMSAPTPPPPPMSVPPPPSAP-PMPAGPPSAPPPPLPASSAPSVPNPG 1747 Score = 27.9 bits (59), Expect = 3.1 Identities = 15/43 (34%), Positives = 16/43 (37%), Gaps = 1/43 (2%) Frame = +1 Query: 418 GXGXXTPSXXXPLXVLSXXPPLGXXPXPPPPXXP-PPXXXLPP 543 G P P+ S PP P PPPP PP PP Sbjct: 1680 GMAPAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPP 1722 Score = 26.6 bits (56), Expect = 7.2 Identities = 16/55 (29%), Positives = 19/55 (34%) Frame = +1 Query: 436 PSXXXPLXVLSXXPPLGXXPXPPPPXXPPPXXXLPPXXPXXXXXXXXFXTRPPPG 600 P P + + PP PPPP PP PP P + P PG Sbjct: 1694 PQSAAPPQMSAPTPPPPPMSVPPPPSA-PPMPAGPPSAPPPPLPASSAPSVPNPG 1747 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 31.1 bits (67), Expect = 0.33 Identities = 12/30 (40%), Positives = 14/30 (46%) Frame = +1 Query: 463 LSXXPPLGXXPXPPPPXXPPPXXXLPPXXP 552 ++ PP P PPPP PP PP P Sbjct: 1 MASLPPGNPPPPPPPPGFEPPSQPPPPPPP 30 Score = 28.3 bits (60), Expect = 2.3 Identities = 11/25 (44%), Positives = 12/25 (48%) Frame = +3 Query: 435 PXSXPPPXRXLVXXPPRXXPXPPPP 509 P + PPP PP P PPPP Sbjct: 6 PGNPPPPPPPPGFEPPSQPPPPPPP 30 >SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 273 Score = 30.7 bits (66), Expect = 0.44 Identities = 23/67 (34%), Positives = 23/67 (34%), Gaps = 4/67 (5%) Frame = -3 Query: 622 GGXXXXPPPGAGGCXKXXSXXXXXXXXGGXXXXG---EGXEGG-GGXGXXRGGXXTRXRX 455 GG PPPG GG GG G G EGG GG G GG Sbjct: 194 GGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGG 253 Query: 454 GGGXEXG 434 GG G Sbjct: 254 FGGGPGG 260 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 29.5 bits (63), Expect = 1.0 Identities = 15/52 (28%), Positives = 17/52 (32%) Frame = +3 Query: 441 SXPPPXRXLVXXPPRXXPXPPPPSXPSPXXXXPPXXXXXXLXXXXFXHPPAP 596 S PPP ++ P P P PP P PP PP P Sbjct: 731 SPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782 Score = 29.5 bits (63), Expect = 1.0 Identities = 17/55 (30%), Positives = 18/55 (32%) Frame = +2 Query: 1055 PPPPQNXTXXXXXXPPSPXXSXSPPXXXXPKXQKXXXPPPAXAXXXKXPXPLPPP 1219 PPPP P+P PP PPP A P P PPP Sbjct: 732 PPPPPPAVIVPT---PAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783 Score = 29.5 bits (63), Expect = 1.0 Identities = 11/23 (47%), Positives = 11/23 (47%) Frame = +3 Query: 1056 PPPPKTXXXXXXANPPPXPXXPP 1124 PPPP A PPP P PP Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPPP 783 Score = 26.6 bits (56), Expect = 7.2 Identities = 16/54 (29%), Positives = 19/54 (35%) Frame = +3 Query: 435 PXSXPPPXRXLVXXPPRXXPXPPPPSXPSPXXXXPPXXXXXXLXXXXFXHPPAP 596 P PPP ++ PP P PPPP PP + PAP Sbjct: 748 PIPVPPP-APIMGGPP---PPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAP 797 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 26.6 bits (56), Expect = 7.2 Identities = 15/45 (33%), Positives = 17/45 (37%), Gaps = 4/45 (8%) Frame = +2 Query: 1097 PPSPXXSXSPPXXXXPKXQKXXXPPPAXAXXXKXP----XPLPPP 1219 PP P S P P PPP+ A P P+PPP Sbjct: 1169 PPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPP 1213 Score = 26.6 bits (56), Expect = 7.2 Identities = 12/29 (41%), Positives = 13/29 (44%), Gaps = 3/29 (10%) Frame = +1 Query: 475 PPLGXXPXPPP---PXXPPPXXXLPPXXP 552 P G P PPP P P P +PP P Sbjct: 1184 PAAGVPPVPPPSEAPPVPKPSVGVPPVPP 1212 Score = 26.6 bits (56), Expect = 7.2 Identities = 12/26 (46%), Positives = 13/26 (50%), Gaps = 3/26 (11%) Frame = +1 Query: 475 PPLGXXPXPPP---PXXPPPXXXLPP 543 P +G P PPP P P P LPP Sbjct: 1203 PSVGVPPVPPPSTAPPVPTPSAGLPP 1228 Score = 26.2 bits (55), Expect = 9.5 Identities = 12/36 (33%), Positives = 13/36 (36%) Frame = +3 Query: 435 PXSXPPPXRXLVXXPPRXXPXPPPPSXPSPXXXXPP 542 P PP + V PP P P P P PP Sbjct: 1155 PSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPP 1190 >SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex protein Gar1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 194 Score = 26.6 bits (56), Expect = 7.2 Identities = 17/53 (32%), Positives = 18/53 (33%) Frame = -2 Query: 632 KEEGGXXXXPPPGGGRVXKXXXXQXXXGXXGGXXXXGGGXXGGGGXGXXPRGG 474 K+ G P GGR G GG G G GG G RGG Sbjct: 125 KKPKGARNGPAGRGGRGGFRGGRGGSRGGFGGNSRGGFGGGSRGGFGGGSRGG 177 >SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr 2|||Manual Length = 305 Score = 26.2 bits (55), Expect = 9.5 Identities = 13/31 (41%), Positives = 13/31 (41%) Frame = -3 Query: 538 GXXXXGEGXEGGGGXGXXRGGXXTRXRXGGG 446 G G G GGG G RGG R GG Sbjct: 26 GGFGGGRGGARGGGRGGARGGRGGRGGARGG 56 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.315 0.146 0.510 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,242,897 Number of Sequences: 5004 Number of extensions: 35824 Number of successful extensions: 264 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 41 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 172 length of database: 2,362,478 effective HSP length: 74 effective length of database: 1,992,182 effective search space used: 677341880 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 42 (21.9 bits)
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