BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_G12
(1245 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 33 0.062
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 33 0.082
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 31 0.33
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 31 0.44
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 29 1.0
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 7.2
SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex p... 27 7.2
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 26 9.5
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 33.5 bits (73), Expect = 0.062
Identities = 23/70 (32%), Positives = 25/70 (35%), Gaps = 6/70 (8%)
Frame = +3
Query: 435 PXSXPPPXRXL---VXXPP--RXXPXPPPP-SXPSPXXXXPPXXXXXXLXXXXFXHPPAP 596
P PPP + PP R P PPPP S PS PP + P P
Sbjct: 337 PPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIP 396
Query: 597 GGGXXXXPPL 626
G PPL
Sbjct: 397 GRSAPALPPL 406
Score = 31.1 bits (67), Expect = 0.33
Identities = 16/55 (29%), Positives = 19/55 (34%)
Frame = +2
Query: 1055 PPPPQNXTXXXXXXPPSPXXSXSPPXXXXPKXQKXXXPPPAXAXXXKXPXPLPPP 1219
PPPP++ PP S PP PPP + P PPP
Sbjct: 340 PPPPRSNAAGSIPLPPQ-GRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPP 393
Score = 27.1 bits (57), Expect = 5.4
Identities = 36/177 (20%), Positives = 42/177 (23%), Gaps = 5/177 (2%)
Frame = +1
Query: 112 PPPPXPXXXLXXPXGKFFXPTPNXLXL*XPXXXSPPPXGGXGKKXKXXGXXXLXPPXXPX 291
PPPP P + N P PP G PP P
Sbjct: 311 PPPPPPPSRRNRGKPPIGNGSSNSSL---PPPPPPPRSNAAGSIPLPPQGRSAPPPPPPR 367
Query: 292 XXPPFSPPPXXXXHKQKXXXPPXTKKNKXXPCXXSSXXQKXXGXGXXTPSXXXPLXVLSX 471
P P + PP P G + + P+
Sbjct: 368 SAPSTGRQPPPLSSSRAVSNPPAPP-----PAIPGRSAPALPPLGNASRTSTPPVPTPPS 422
Query: 472 XPPLGXXPXPP--PPXXP---PPXXXLPPXXPXXXXXXXXFXTRPPPGGGXNXXPPS 627
PP PP PP P P LPP P PP PP+
Sbjct: 423 LPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPA 479
Score = 26.2 bits (55), Expect = 9.5
Identities = 11/30 (36%), Positives = 12/30 (40%)
Frame = +3
Query: 435 PXSXPPPXRXLVXXPPRXXPXPPPPSXPSP 524
P PPP V PP P P P +P
Sbjct: 253 PPPIPPPSNGTVSSPPNSPPRPIAPVSMNP 282
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 33.1 bits (72), Expect = 0.082
Identities = 19/55 (34%), Positives = 19/55 (34%)
Frame = +3
Query: 435 PXSXPPPXRXLVXXPPRXXPXPPPPSXPSPXXXXPPXXXXXXLXXXXFXHPPAPG 599
P S PP PP PPPPS P P PP L P PG
Sbjct: 1694 PQSAAPPQMSAPTPPPPPMSVPPPPSAP-PMPAGPPSAPPPPLPASSAPSVPNPG 1747
Score = 27.9 bits (59), Expect = 3.1
Identities = 15/43 (34%), Positives = 16/43 (37%), Gaps = 1/43 (2%)
Frame = +1
Query: 418 GXGXXTPSXXXPLXVLSXXPPLGXXPXPPPPXXP-PPXXXLPP 543
G P P+ S PP P PPPP PP PP
Sbjct: 1680 GMAPAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPP 1722
Score = 26.6 bits (56), Expect = 7.2
Identities = 16/55 (29%), Positives = 19/55 (34%)
Frame = +1
Query: 436 PSXXXPLXVLSXXPPLGXXPXPPPPXXPPPXXXLPPXXPXXXXXXXXFXTRPPPG 600
P P + + PP PPPP PP PP P + P PG
Sbjct: 1694 PQSAAPPQMSAPTPPPPPMSVPPPPSA-PPMPAGPPSAPPPPLPASSAPSVPNPG 1747
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 31.1 bits (67), Expect = 0.33
Identities = 12/30 (40%), Positives = 14/30 (46%)
Frame = +1
Query: 463 LSXXPPLGXXPXPPPPXXPPPXXXLPPXXP 552
++ PP P PPPP PP PP P
Sbjct: 1 MASLPPGNPPPPPPPPGFEPPSQPPPPPPP 30
Score = 28.3 bits (60), Expect = 2.3
Identities = 11/25 (44%), Positives = 12/25 (48%)
Frame = +3
Query: 435 PXSXPPPXRXLVXXPPRXXPXPPPP 509
P + PPP PP P PPPP
Sbjct: 6 PGNPPPPPPPPGFEPPSQPPPPPPP 30
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 30.7 bits (66), Expect = 0.44
Identities = 23/67 (34%), Positives = 23/67 (34%), Gaps = 4/67 (5%)
Frame = -3
Query: 622 GGXXXXPPPGAGGCXKXXSXXXXXXXXGGXXXXG---EGXEGG-GGXGXXRGGXXTRXRX 455
GG PPPG GG GG G G EGG GG G GG
Sbjct: 194 GGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGG 253
Query: 454 GGGXEXG 434
GG G
Sbjct: 254 FGGGPGG 260
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 29.5 bits (63), Expect = 1.0
Identities = 15/52 (28%), Positives = 17/52 (32%)
Frame = +3
Query: 441 SXPPPXRXLVXXPPRXXPXPPPPSXPSPXXXXPPXXXXXXLXXXXFXHPPAP 596
S PPP ++ P P P PP P PP PP P
Sbjct: 731 SPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 29.5 bits (63), Expect = 1.0
Identities = 17/55 (30%), Positives = 18/55 (32%)
Frame = +2
Query: 1055 PPPPQNXTXXXXXXPPSPXXSXSPPXXXXPKXQKXXXPPPAXAXXXKXPXPLPPP 1219
PPPP P+P PP PPP A P P PPP
Sbjct: 732 PPPPPPAVIVPT---PAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 29.5 bits (63), Expect = 1.0
Identities = 11/23 (47%), Positives = 11/23 (47%)
Frame = +3
Query: 1056 PPPPKTXXXXXXANPPPXPXXPP 1124
PPPP A PPP P PP
Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPPP 783
Score = 26.6 bits (56), Expect = 7.2
Identities = 16/54 (29%), Positives = 19/54 (35%)
Frame = +3
Query: 435 PXSXPPPXRXLVXXPPRXXPXPPPPSXPSPXXXXPPXXXXXXLXXXXFXHPPAP 596
P PPP ++ PP P PPPP PP + PAP
Sbjct: 748 PIPVPPP-APIMGGPP---PPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAP 797
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 26.6 bits (56), Expect = 7.2
Identities = 15/45 (33%), Positives = 17/45 (37%), Gaps = 4/45 (8%)
Frame = +2
Query: 1097 PPSPXXSXSPPXXXXPKXQKXXXPPPAXAXXXKXP----XPLPPP 1219
PP P S P P PPP+ A P P+PPP
Sbjct: 1169 PPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPP 1213
Score = 26.6 bits (56), Expect = 7.2
Identities = 12/29 (41%), Positives = 13/29 (44%), Gaps = 3/29 (10%)
Frame = +1
Query: 475 PPLGXXPXPPP---PXXPPPXXXLPPXXP 552
P G P PPP P P P +PP P
Sbjct: 1184 PAAGVPPVPPPSEAPPVPKPSVGVPPVPP 1212
Score = 26.6 bits (56), Expect = 7.2
Identities = 12/26 (46%), Positives = 13/26 (50%), Gaps = 3/26 (11%)
Frame = +1
Query: 475 PPLGXXPXPPP---PXXPPPXXXLPP 543
P +G P PPP P P P LPP
Sbjct: 1203 PSVGVPPVPPPSTAPPVPTPSAGLPP 1228
Score = 26.2 bits (55), Expect = 9.5
Identities = 12/36 (33%), Positives = 13/36 (36%)
Frame = +3
Query: 435 PXSXPPPXRXLVXXPPRXXPXPPPPSXPSPXXXXPP 542
P PP + V PP P P P P PP
Sbjct: 1155 PSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPP 1190
>SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex
protein Gar1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 194
Score = 26.6 bits (56), Expect = 7.2
Identities = 17/53 (32%), Positives = 18/53 (33%)
Frame = -2
Query: 632 KEEGGXXXXPPPGGGRVXKXXXXQXXXGXXGGXXXXGGGXXGGGGXGXXPRGG 474
K+ G P GGR G GG G G GG G RGG
Sbjct: 125 KKPKGARNGPAGRGGRGGFRGGRGGSRGGFGGNSRGGFGGGSRGGFGGGSRGG 177
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 26.2 bits (55), Expect = 9.5
Identities = 13/31 (41%), Positives = 13/31 (41%)
Frame = -3
Query: 538 GXXXXGEGXEGGGGXGXXRGGXXTRXRXGGG 446
G G G GGG G RGG R GG
Sbjct: 26 GGFGGGRGGARGGGRGGARGGRGGRGGARGG 56
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.315 0.146 0.510
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,242,897
Number of Sequences: 5004
Number of extensions: 35824
Number of successful extensions: 264
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 41
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 74
effective length of database: 1,992,182
effective search space used: 677341880
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
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