Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_G10
(877 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 52 1e-07
SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 46 7e-06
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 41 2e-04
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 40 6e-04
SPAC13D6.02c |byr3||zinc finger protein Byr3|Schizosaccharomyces... 34 0.031
SPBP35G2.08c |air1||zinc knuckle TRAMP complex subunit Air1|Schi... 32 0.093
SPAC29B12.12 |||helper of TIM |Schizosaccharomyces pombe|chr 1||... 27 2.7
SPBP35G2.13c |swc2||chromatin remodeling complex subunit Swc2 |S... 26 6.1
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 51.6 bits (118), Expect = 1e-07
Identities = 21/55 (38%), Positives = 32/55 (58%), Gaps = 2/55 (3%)
Frame = +3
Query: 501 CPRCGGYVYAAEQMLARGRAWHKECFKCGDCMKRLD--STNCCEGSDKDIYCKVC 659
C +C V + Q+ G+ WH +CFKC +C K+LD S + + K I+CK+C
Sbjct: 24 CIKCWESVPSTSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKLC 78
Score = 46.0 bits (104), Expect = 7e-06
Identities = 20/55 (36%), Positives = 31/55 (56%), Gaps = 3/55 (5%)
Frame = +3
Query: 180 CPKCGKSVYAAEERVAGGLKWHKMCFKCGLCQKLLDSTN---CSEHEGELYCKVC 335
C KC +SV + + GG WH CFKC C K LD ++ + + +++CK+C
Sbjct: 24 CIKCWESVPSTSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKLC 78
>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 46.0 bits (104), Expect = 7e-06
Identities = 23/71 (32%), Positives = 32/71 (45%), Gaps = 2/71 (2%)
Frame = +3
Query: 144 STMP--FKPADNPKCPKCGKSVYAAEERVAGGLKWHKMCFKCGLCQKLLDSTNCSEHEGE 317
ST P ++ C CG S+ A A G K H CFKC C + L+ EG+
Sbjct: 244 STKPVLYRGNSEKSCHSCGGSLRAGRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGK 303
Query: 318 LYCKVCHARKF 350
YC + + +F
Sbjct: 304 FYCHLDYHEQF 314
Score = 37.9 bits (84), Expect = 0.002
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +3
Query: 495 EGCPRCGGYVYAAEQMLARGRAWHKECFKCGDCMKRLD 608
+ C CGG + A + A G+ H +CFKC C + L+
Sbjct: 256 KSCHSCGGSLRAGRIISASGKKLHPQCFKCDTCSQNLE 293
Score = 37.1 bits (82), Expect = 0.003
Identities = 17/55 (30%), Positives = 24/55 (43%)
Frame = +3
Query: 177 KCPKCGKSVYAAEERVAGGLKWHKMCFKCGLCQKLLDSTNCSEHEGELYCKVCHA 341
KC KC K + + + G ++H C+ CG C LL E C+ C A
Sbjct: 377 KCKKCRKPILGISVKGSDG-EYHSQCWTCGACNALLGDEGYFMIENTPICRPCKA 430
>SPAC29A4.11 |rga3||GTPase activating protein
Rga3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 969
Score = 41.1 bits (92), Expect = 2e-04
Identities = 24/70 (34%), Positives = 35/70 (50%), Gaps = 6/70 (8%)
Frame = +3
Query: 468 RSIAKAPPGEG---CPRCGGYVYAAEQMLA-RGRAWHKECFKCGDCMKRLDSTN--CCEG 629
+SI+K+P +G C RCG E ++ G WHK+CF C C K L+ ++ +
Sbjct: 5 KSISKSPSSKGSTVCFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQT 64
Query: 630 SDKDIYCKVC 659
SD C C
Sbjct: 65 SDGRPVCSSC 74
Score = 36.3 bits (80), Expect = 0.006
Identities = 18/56 (32%), Positives = 27/56 (48%), Gaps = 4/56 (7%)
Frame = +3
Query: 180 CPKCGKSVYAAEERVA-GGLKWHKMCFKCGLCQKLLDSTN---CSEHEGELYCKVC 335
C +CG++ E ++ GG WHK CF C C K L+ ++ +G C C
Sbjct: 19 CFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSC 74
Score = 35.5 bits (78), Expect = 0.010
Identities = 12/34 (35%), Positives = 23/34 (67%)
Frame = +3
Query: 558 AWHKECFKCGDCMKRLDSTNCCEGSDKDIYCKVC 659
++H+ECF+C DC K++ +N + ++ I+C C
Sbjct: 96 SYHRECFRCHDCRKQIIDSN-FKRDNRTIFCNDC 128
Score = 32.7 bits (71), Expect = 0.071
Identities = 15/60 (25%), Positives = 26/60 (43%), Gaps = 6/60 (10%)
Frame = +3
Query: 174 PKCPKCGKSVYAAEERV------AGGLKWHKMCFKCGLCQKLLDSTNCSEHEGELYCKVC 335
P C C + A R+ +G +H+ CF+C C+K + +N ++C C
Sbjct: 69 PVCSSCAHTCTACRMRIKDYALMSGYDSYHRECFRCHDCRKQIIDSNFKRDNRTIFCNDC 128
>SPBC3F6.05 |rga1||GTPase activating protein
Rga1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1150
Score = 39.5 bits (88), Expect = 6e-04
Identities = 17/60 (28%), Positives = 30/60 (50%)
Frame = +3
Query: 414 LKGENAGGVRTNGACLEPRSIAKAPPGEGCPRCGGYVYAAEQMLARGRAWHKECFKCGDC 593
L E ++ + + P+++ K + C CG V + + + A G +H ECF+C DC
Sbjct: 87 LNKEEQQSLKRSDTSVFPKAVRKVSSSKICASCG-QVISGQYVRALGNIYHLECFRCHDC 145
>SPAC13D6.02c |byr3||zinc finger protein Byr3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 179
Score = 33.9 bits (74), Expect = 0.031
Identities = 42/175 (24%), Positives = 60/175 (34%), Gaps = 4/175 (2%)
Frame = +3
Query: 147 TMPFKPADNPKCPKCGKSVYAAEERVAGGLKWHKMCFKCGLCQKLLDSTNCSEHEGELYC 326
T+P P+C CG++ + A E G + C+ C Q ++ C+E + E C
Sbjct: 8 TVPQTTRPGPRCYNCGENGHQARECTKGSI-----CYNCN--QTGHKASECTEPQQEKTC 60
Query: 327 KVC----HARKFXXXXXXXXXXXXCLSMDTGDHLKGENAGGVRTNGACLEPRSIAKAPPG 494
C H + C H+ A RTNG R
Sbjct: 61 YACGTAGHLVRDCPSSPNPRQGAECYKCGRVGHI----ARDCRTNGQQSGGR-FGGHRSN 115
Query: 495 EGCPRCGGYVYAAEQMLARGRAWHKECFKCGDCMKRLDSTNCCEGSDKDIYCKVC 659
C CG Y + AR +C+ CG R S C + SD + C C
Sbjct: 116 MNCYACGSYGHQ-----ARDCTMGVKCYSCGKIGHR--SFECQQASDGQL-CYKC 162
>SPBP35G2.08c |air1||zinc knuckle TRAMP complex subunit
Air1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 313
Score = 32.3 bits (70), Expect = 0.093
Identities = 17/55 (30%), Positives = 24/55 (43%), Gaps = 2/55 (3%)
Frame = +3
Query: 180 CPKCGKSVYAAEERVAGGLKWHKMCFKCGLCQKLLDSTNCSE--HEGELYCKVCH 338
C CG A ++ ++ W K C CGL + + CSE G C+ CH
Sbjct: 107 CTTCG----AIDDHISVRCPWTKKCMNCGLLGHI--AARCSEPRKRGPRVCRTCH 155
>SPAC29B12.12 |||helper of TIM |Schizosaccharomyces pombe|chr
1|||Manual
Length = 113
Score = 27.5 bits (58), Expect = 2.7
Identities = 11/34 (32%), Positives = 18/34 (52%)
Frame = +3
Query: 237 KWHKMCFKCGLCQKLLDSTNCSEHEGELYCKVCH 338
K+H C CG C+ +S E+ ++CK C+
Sbjct: 65 KFHIPCVICGACK---NSLTVEEYRSTVHCKYCN 95
>SPBP35G2.13c |swc2||chromatin remodeling complex subunit Swc2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 316
Score = 26.2 bits (55), Expect = 6.1
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = -1
Query: 289 ESSNFWHRPHLKHILCHLRPP 227
E+SN++ P L+H LCH PP
Sbjct: 241 EASNYYVAP-LEHPLCHSAPP 260
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,276,296
Number of Sequences: 5004
Number of extensions: 60908
Number of successful extensions: 165
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 165
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 438479610
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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