BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_G01
(851 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 35 0.013
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 33 0.068
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.21
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 30 0.34
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 29 0.63
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 27 4.5
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 4.5
SPBC11B10.06 |sws1||SWIM domain containing-Srs2 interacting prot... 26 5.9
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 26 5.9
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 35.1 bits (77), Expect = 0.013
Identities = 23/78 (29%), Positives = 24/78 (30%)
Frame = +3
Query: 582 PPPPSXXXXPPXXXPSPXXXXXXXPPPLXXXXXPXXXXXXKXXPPPPXPXXXXXXXPPXX 761
PPPP P P+P PPP P PPPP P PP
Sbjct: 732 PPPPP----PAVIVPTPAPAPIPVPPPAPIMGGPP--------PPPPPPGVAGAGPPPPP 779
Query: 762 XKPXXXXGGGXXXXXPPP 815
P GG P P
Sbjct: 780 PPPPAVSAGGSRYYAPAP 797
Score = 31.5 bits (68), Expect = 0.16
Identities = 18/52 (34%), Positives = 18/52 (34%), Gaps = 6/52 (11%)
Frame = +2
Query: 701 KXPPPPPXXXXXPPXXPPXXXXTPXXXGGGGAXXXXXPP------PPPXXPP 838
K PPPPP P P P GG PP PPP PP
Sbjct: 730 KSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 30.7 bits (66), Expect = 0.27
Identities = 17/50 (34%), Positives = 17/50 (34%), Gaps = 6/50 (12%)
Frame = +2
Query: 707 PPPPPXXXXXPPXXPPXXXXTPXXXGGGGAXXXXXP------PPPPXXPP 838
PPPPP P P P GG P PPPP PP
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 29.5 bits (63), Expect = 0.63
Identities = 16/44 (36%), Positives = 16/44 (36%)
Frame = +2
Query: 707 PPPPPXXXXXPPXXPPXXXXTPXXXGGGGAXXXXXPPPPPXXPP 838
P P P PP P P G A PPPPP PP
Sbjct: 742 PTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP--PP 783
Score = 28.3 bits (60), Expect = 1.5
Identities = 17/62 (27%), Positives = 17/62 (27%)
Frame = +1
Query: 655 PXPXXXXXXXPXXXXXXXXPPPPXPPXXXXXPPXXXXNPXXXRGGGXXXXXXPPPPPXXP 834
P P P PPPP P PP P GG P P P
Sbjct: 744 PAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGG--SRYYAPAPQAEP 801
Query: 835 XP 840
P
Sbjct: 802 EP 803
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 32.7 bits (71), Expect = 0.068
Identities = 26/95 (27%), Positives = 26/95 (27%), Gaps = 5/95 (5%)
Frame = +3
Query: 582 PPPPSXXXXPPXXXPS----PXXXXXXXPP-PLXXXXXPXXXXXXKXXPPPPXPXXXXXX 746
PP P PP PS P PP P P PP P P
Sbjct: 1131 PPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPP 1190
Query: 747 XPPXXXKPXXXXGGGXXXXXPPPPPPXXXXPXXXP 851
PP P PP PPP P P
Sbjct: 1191 VPPPSEAPPVPK---PSVGVPPVPPPSTAPPVPTP 1222
Score = 28.3 bits (60), Expect = 1.5
Identities = 22/85 (25%), Positives = 22/85 (25%), Gaps = 1/85 (1%)
Frame = +3
Query: 588 PPSXXXXPPXXXPSPXXXXXXXPP-PLXXXXXPXXXXXXKXXPPPPXPXXXXXXXPPXXX 764
PPS P SP PP P P PP P P P
Sbjct: 991 PPSAPLSKPVST-SPAAPLARVPPVPKLSSKAPPVPLPSADAPPIPVPSTAPPVPIPTST 1049
Query: 765 KPXXXXGGGXXXXXPPPPPPXXXXP 839
P G PP P P P
Sbjct: 1050 PPVPKSSSGAPSAPPPVPAPSSEIP 1074
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 31.1 bits (67), Expect = 0.21
Identities = 13/34 (38%), Positives = 13/34 (38%)
Frame = +3
Query: 582 PPPPSXXXXPPXXXPSPXXXXXXXPPPLXXXXXP 683
PPPP PP P P PPPL P
Sbjct: 1708 PPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAP 1741
Score = 27.5 bits (58), Expect = 2.6
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = +2
Query: 707 PPPPPXXXXXPPXXPPXXXXTP 772
PPPPP PP PP P
Sbjct: 1707 PPPPPMSVPPPPSAPPMPAGPP 1728
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 30.3 bits (65), Expect = 0.36
Identities = 27/96 (28%), Positives = 28/96 (29%), Gaps = 11/96 (11%)
Frame = +2
Query: 584 PPPXXXXPPPXXGXXSXXXPXXXXPXPXXXXXXXXXXXXKXPPPPPXXXXX--------- 736
PPP PPP G S P K P PPP
Sbjct: 253 PPPI---PPPSNGTVSSPPNSPPRPIAPVSMNPAINSTSKPPLPPPSSRVSAAALAANKK 309
Query: 737 --PPXXPPXXXXTPXXXGGGGAXXXXXPPPPPXXPP 838
PP PP G G+ PPPPP PP
Sbjct: 310 RPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPP--PP 343
Score = 29.9 bits (64), Expect = 0.48
Identities = 24/93 (25%), Positives = 24/93 (25%)
Frame = +3
Query: 573 GXXPPPPSXXXXPPXXXPSPXXXXXXXPPPLXXXXXPXXXXXXKXXPPPPXPXXXXXXXP 752
G P PP PP P PPPL PPP P P
Sbjct: 349 GSIPLPPQGRSAPPPPPPRSAPSTGRQPPPL----SSSRAVSNPPAPPPAIPGRSAPALP 404
Query: 753 PXXXKPXXXXGGGXXXXXPPPPPPXXXXPXXXP 851
P G PP P P P P
Sbjct: 405 P--------LGNASRTSTPPVPTPPSLPPSAPP 429
Score = 27.9 bits (59), Expect(2) = 0.34
Identities = 25/94 (26%), Positives = 25/94 (26%), Gaps = 4/94 (4%)
Frame = +2
Query: 581 PPP--PXXXXP--PPXXGXXSXXXPXXXXPXPXXXXXXXXXXXXKXPPPPPXXXXXPPXX 748
PPP P P PP P P P PP P P
Sbjct: 391 PPPAIPGRSAPALPPLGNASRTSTPPVPTP-PSLPPSAPPSLPPSAPPSLPMGAPAAPPL 449
Query: 749 PPXXXXTPXXXGGGGAXXXXXPPPPPXXPPXXXP 850
PP P G A PP P PP P
Sbjct: 450 PPSAPIAPPLPAGMPA-APPLPPAAPAPPPAPAP 482
Score = 21.0 bits (42), Expect(2) = 0.34
Identities = 9/25 (36%), Positives = 9/25 (36%)
Frame = +2
Query: 395 PPFSXFFXPXPXPXFFPPKXXXPPP 469
PP P P P P PPP
Sbjct: 354 PPQGRSAPPPPPPRSAPSTGRQPPP 378
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 29.5 bits (63), Expect = 0.63
Identities = 25/83 (30%), Positives = 25/83 (30%), Gaps = 2/83 (2%)
Frame = -2
Query: 823 GGGGGGXXXXXPPPXXXXGXXXXG--GXXXXXXXGXGGGGXXFXXXXXXGXXXXXRGGGX 650
GG GGG P P G G G G GGG F G G G
Sbjct: 191 GGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGF----EGGPGGFGGGPGG 246
Query: 649 XXXXXXGXGXXXGGXXXXXGGGG 581
G G GG GG G
Sbjct: 247 FGGGLGGFGGGPGGFGGGPGGHG 269
Score = 29.1 bits (62), Expect = 0.84
Identities = 26/86 (30%), Positives = 26/86 (30%), Gaps = 2/86 (2%)
Frame = -3
Query: 834 GXXGGG-GGXXXXXAPPPPXXXGVXXXXG-GXXGGXXXXXGGGGGXXXXXXXXXXXXXXG 661
G GGG GG PPP G G G G GG GG G
Sbjct: 184 GHNGGGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGG 243
Query: 660 XGXXXXGXXKEXXPXXGGGXXXXGGG 583
G G GGG GGG
Sbjct: 244 PGGFGGGLG-----GFGGGPGGFGGG 264
Score = 26.6 bits (56), Expect = 4.5
Identities = 17/48 (35%), Positives = 17/48 (35%)
Frame = -3
Query: 849 GXXXGGXXGGGGGXXXXXAPPPPXXXGVXXXXGGXXGGXXXXXGGGGG 706
G GG G GGG P G GG GG GG GG
Sbjct: 217 GHHHGGHGGFGGGPGGFEGGP----GGFGGGPGGFGGGLGGFGGGPGG 260
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 26.6 bits (56), Expect = 4.5
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +2
Query: 707 PPPPPXXXXXPPXXPP 754
PPPPP PP PP
Sbjct: 10 PPPPPPPGFEPPSQPP 25
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.6 bits (56), Expect = 4.5
Identities = 13/48 (27%), Positives = 13/48 (27%)
Frame = +2
Query: 707 PPPPPXXXXXPPXXPPXXXXTPXXXGGGGAXXXXXPPPPPXXPPXXXP 850
PP P P PP P PP P PP P
Sbjct: 130 PPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQP 177
>SPBC11B10.06 |sws1||SWIM domain containing-Srs2 interacting protein
1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 209
Score = 26.2 bits (55), Expect = 5.9
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = -3
Query: 90 LKIIISSLFDLGQRKILRNSPNSEL 16
++ +ISSL D Q K L+ SPN+ L
Sbjct: 58 IRSVISSLLDSLQDKALQQSPNTSL 82
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 26.2 bits (55), Expect = 5.9
Identities = 12/36 (33%), Positives = 15/36 (41%)
Frame = -3
Query: 483 GXGPXGGGXXFLGGKKXGXGXGXKNXEKGGXXXXGG 376
G G GG GG + G G + +GG GG
Sbjct: 17 GRGGFNGGRGGFGGGRGGARGGGRGGARGGRGGRGG 52
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,269,325
Number of Sequences: 5004
Number of extensions: 39102
Number of successful extensions: 237
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 59
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 422462090
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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