BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP03_F_G01 (851 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 35 0.013 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 33 0.068 SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.21 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 30 0.34 SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 29 0.63 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 27 4.5 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 4.5 SPBC11B10.06 |sws1||SWIM domain containing-Srs2 interacting prot... 26 5.9 SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 26 5.9 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 35.1 bits (77), Expect = 0.013 Identities = 23/78 (29%), Positives = 24/78 (30%) Frame = +3 Query: 582 PPPPSXXXXPPXXXPSPXXXXXXXPPPLXXXXXPXXXXXXKXXPPPPXPXXXXXXXPPXX 761 PPPP P P+P PPP P PPPP P PP Sbjct: 732 PPPPP----PAVIVPTPAPAPIPVPPPAPIMGGPP--------PPPPPPGVAGAGPPPPP 779 Query: 762 XKPXXXXGGGXXXXXPPP 815 P GG P P Sbjct: 780 PPPPAVSAGGSRYYAPAP 797 Score = 31.5 bits (68), Expect = 0.16 Identities = 18/52 (34%), Positives = 18/52 (34%), Gaps = 6/52 (11%) Frame = +2 Query: 701 KXPPPPPXXXXXPPXXPPXXXXTPXXXGGGGAXXXXXPP------PPPXXPP 838 K PPPPP P P P GG PP PPP PP Sbjct: 730 KSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781 Score = 30.7 bits (66), Expect = 0.27 Identities = 17/50 (34%), Positives = 17/50 (34%), Gaps = 6/50 (12%) Frame = +2 Query: 707 PPPPPXXXXXPPXXPPXXXXTPXXXGGGGAXXXXXP------PPPPXXPP 838 PPPPP P P P GG P PPPP PP Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782 Score = 29.5 bits (63), Expect = 0.63 Identities = 16/44 (36%), Positives = 16/44 (36%) Frame = +2 Query: 707 PPPPPXXXXXPPXXPPXXXXTPXXXGGGGAXXXXXPPPPPXXPP 838 P P P PP P P G A PPPPP PP Sbjct: 742 PTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP--PP 783 Score = 28.3 bits (60), Expect = 1.5 Identities = 17/62 (27%), Positives = 17/62 (27%) Frame = +1 Query: 655 PXPXXXXXXXPXXXXXXXXPPPPXPPXXXXXPPXXXXNPXXXRGGGXXXXXXPPPPPXXP 834 P P P PPPP P PP P GG P P P Sbjct: 744 PAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGG--SRYYAPAPQAEP 801 Query: 835 XP 840 P Sbjct: 802 EP 803 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 32.7 bits (71), Expect = 0.068 Identities = 26/95 (27%), Positives = 26/95 (27%), Gaps = 5/95 (5%) Frame = +3 Query: 582 PPPPSXXXXPPXXXPS----PXXXXXXXPP-PLXXXXXPXXXXXXKXXPPPPXPXXXXXX 746 PP P PP PS P PP P P PP P P Sbjct: 1131 PPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPP 1190 Query: 747 XPPXXXKPXXXXGGGXXXXXPPPPPPXXXXPXXXP 851 PP P PP PPP P P Sbjct: 1191 VPPPSEAPPVPK---PSVGVPPVPPPSTAPPVPTP 1222 Score = 28.3 bits (60), Expect = 1.5 Identities = 22/85 (25%), Positives = 22/85 (25%), Gaps = 1/85 (1%) Frame = +3 Query: 588 PPSXXXXPPXXXPSPXXXXXXXPP-PLXXXXXPXXXXXXKXXPPPPXPXXXXXXXPPXXX 764 PPS P SP PP P P PP P P P Sbjct: 991 PPSAPLSKPVST-SPAAPLARVPPVPKLSSKAPPVPLPSADAPPIPVPSTAPPVPIPTST 1049 Query: 765 KPXXXXGGGXXXXXPPPPPPXXXXP 839 P G PP P P P Sbjct: 1050 PPVPKSSSGAPSAPPPVPAPSSEIP 1074 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 31.1 bits (67), Expect = 0.21 Identities = 13/34 (38%), Positives = 13/34 (38%) Frame = +3 Query: 582 PPPPSXXXXPPXXXPSPXXXXXXXPPPLXXXXXP 683 PPPP PP P P PPPL P Sbjct: 1708 PPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAP 1741 Score = 27.5 bits (58), Expect = 2.6 Identities = 10/22 (45%), Positives = 10/22 (45%) Frame = +2 Query: 707 PPPPPXXXXXPPXXPPXXXXTP 772 PPPPP PP PP P Sbjct: 1707 PPPPPMSVPPPPSAPPMPAGPP 1728 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 30.3 bits (65), Expect = 0.36 Identities = 27/96 (28%), Positives = 28/96 (29%), Gaps = 11/96 (11%) Frame = +2 Query: 584 PPPXXXXPPPXXGXXSXXXPXXXXPXPXXXXXXXXXXXXKXPPPPPXXXXX--------- 736 PPP PPP G S P K P PPP Sbjct: 253 PPPI---PPPSNGTVSSPPNSPPRPIAPVSMNPAINSTSKPPLPPPSSRVSAAALAANKK 309 Query: 737 --PPXXPPXXXXTPXXXGGGGAXXXXXPPPPPXXPP 838 PP PP G G+ PPPPP PP Sbjct: 310 RPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPP--PP 343 Score = 29.9 bits (64), Expect = 0.48 Identities = 24/93 (25%), Positives = 24/93 (25%) Frame = +3 Query: 573 GXXPPPPSXXXXPPXXXPSPXXXXXXXPPPLXXXXXPXXXXXXKXXPPPPXPXXXXXXXP 752 G P PP PP P PPPL PPP P P Sbjct: 349 GSIPLPPQGRSAPPPPPPRSAPSTGRQPPPL----SSSRAVSNPPAPPPAIPGRSAPALP 404 Query: 753 PXXXKPXXXXGGGXXXXXPPPPPPXXXXPXXXP 851 P G PP P P P P Sbjct: 405 P--------LGNASRTSTPPVPTPPSLPPSAPP 429 Score = 27.9 bits (59), Expect(2) = 0.34 Identities = 25/94 (26%), Positives = 25/94 (26%), Gaps = 4/94 (4%) Frame = +2 Query: 581 PPP--PXXXXP--PPXXGXXSXXXPXXXXPXPXXXXXXXXXXXXKXPPPPPXXXXXPPXX 748 PPP P P PP P P P PP P P Sbjct: 391 PPPAIPGRSAPALPPLGNASRTSTPPVPTP-PSLPPSAPPSLPPSAPPSLPMGAPAAPPL 449 Query: 749 PPXXXXTPXXXGGGGAXXXXXPPPPPXXPPXXXP 850 PP P G A PP P PP P Sbjct: 450 PPSAPIAPPLPAGMPA-APPLPPAAPAPPPAPAP 482 Score = 21.0 bits (42), Expect(2) = 0.34 Identities = 9/25 (36%), Positives = 9/25 (36%) Frame = +2 Query: 395 PPFSXFFXPXPXPXFFPPKXXXPPP 469 PP P P P P PPP Sbjct: 354 PPQGRSAPPPPPPRSAPSTGRQPPP 378 >SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 273 Score = 29.5 bits (63), Expect = 0.63 Identities = 25/83 (30%), Positives = 25/83 (30%), Gaps = 2/83 (2%) Frame = -2 Query: 823 GGGGGGXXXXXPPPXXXXGXXXXG--GXXXXXXXGXGGGGXXFXXXXXXGXXXXXRGGGX 650 GG GGG P P G G G G GGG F G G G Sbjct: 191 GGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGF----EGGPGGFGGGPGG 246 Query: 649 XXXXXXGXGXXXGGXXXXXGGGG 581 G G GG GG G Sbjct: 247 FGGGLGGFGGGPGGFGGGPGGHG 269 Score = 29.1 bits (62), Expect = 0.84 Identities = 26/86 (30%), Positives = 26/86 (30%), Gaps = 2/86 (2%) Frame = -3 Query: 834 GXXGGG-GGXXXXXAPPPPXXXGVXXXXG-GXXGGXXXXXGGGGGXXXXXXXXXXXXXXG 661 G GGG GG PPP G G G G GG GG G Sbjct: 184 GHNGGGFGGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGG 243 Query: 660 XGXXXXGXXKEXXPXXGGGXXXXGGG 583 G G GGG GGG Sbjct: 244 PGGFGGGLG-----GFGGGPGGFGGG 264 Score = 26.6 bits (56), Expect = 4.5 Identities = 17/48 (35%), Positives = 17/48 (35%) Frame = -3 Query: 849 GXXXGGXXGGGGGXXXXXAPPPPXXXGVXXXXGGXXGGXXXXXGGGGG 706 G GG G GGG P G GG GG GG GG Sbjct: 217 GHHHGGHGGFGGGPGGFEGGP----GGFGGGPGGFGGGLGGFGGGPGG 260 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 26.6 bits (56), Expect = 4.5 Identities = 9/16 (56%), Positives = 9/16 (56%) Frame = +2 Query: 707 PPPPPXXXXXPPXXPP 754 PPPPP PP PP Sbjct: 10 PPPPPPPGFEPPSQPP 25 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.6 bits (56), Expect = 4.5 Identities = 13/48 (27%), Positives = 13/48 (27%) Frame = +2 Query: 707 PPPPPXXXXXPPXXPPXXXXTPXXXGGGGAXXXXXPPPPPXXPPXXXP 850 PP P P PP P PP P PP P Sbjct: 130 PPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQP 177 >SPBC11B10.06 |sws1||SWIM domain containing-Srs2 interacting protein 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 209 Score = 26.2 bits (55), Expect = 5.9 Identities = 12/25 (48%), Positives = 17/25 (68%) Frame = -3 Query: 90 LKIIISSLFDLGQRKILRNSPNSEL 16 ++ +ISSL D Q K L+ SPN+ L Sbjct: 58 IRSVISSLLDSLQDKALQQSPNTSL 82 >SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr 2|||Manual Length = 305 Score = 26.2 bits (55), Expect = 5.9 Identities = 12/36 (33%), Positives = 15/36 (41%) Frame = -3 Query: 483 GXGPXGGGXXFLGGKKXGXGXGXKNXEKGGXXXXGG 376 G G GG GG + G G + +GG GG Sbjct: 17 GRGGFNGGRGGFGGGRGGARGGGRGGARGGRGGRGG 52 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,269,325 Number of Sequences: 5004 Number of extensions: 39102 Number of successful extensions: 237 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 59 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 148 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 422462090 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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