Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_F23
(901 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g47930.1 68418.m05921 40S ribosomal protein S27 (RPS27D) 138 4e-33
At3g61110.1 68416.m06839 40S ribosomal protein S27 (ARS27A) iden... 135 3e-32
At2g45710.1 68415.m05685 40S ribosomal protein S27 (RPS27A) 130 2e-30
At1g59910.1 68414.m06749 formin homology 2 domain-containing pro... 32 0.60
At4g21100.1 68417.m03051 UV-damaged DNA-binding protein, putativ... 31 0.79
At5g62640.1 68418.m07862 proline-rich family protein contains pr... 30 1.8
At5g54650.2 68418.m06805 formin homology 2 domain-containing pro... 30 1.8
At5g54650.1 68418.m06804 formin homology 2 domain-containing pro... 30 1.8
At2g44870.1 68415.m05586 expressed protein 30 1.8
At1g61080.1 68414.m06877 proline-rich family protein 30 1.8
At4g13340.1 68417.m02084 leucine-rich repeat family protein / ex... 30 2.4
At1g31810.1 68414.m03904 formin homology 2 domain-containing pro... 30 2.4
At3g22800.1 68416.m02874 leucine-rich repeat family protein / ex... 29 5.6
At2g33845.1 68415.m04154 DNA-binding protein-related contains we... 29 5.6
At1g49750.1 68414.m05579 leucine-rich repeat family protein cont... 29 5.6
At4g18570.1 68417.m02749 proline-rich family protein common fami... 28 7.3
At3g25690.1 68416.m03197 hydroxyproline-rich glycoprotein family... 28 7.3
At5g07780.1 68418.m00890 formin homology 2 domain-containing pro... 28 9.7
>At5g47930.1 68418.m05921 40S ribosomal protein S27 (RPS27D)
Length = 84
Score = 138 bits (335), Expect = 4e-33
Identities = 59/78 (75%), Positives = 66/78 (84%)
Frame = +3
Query: 87 IDLLHPSPASERRKHKLKRLVPHPNSYFMDVKCPGCYKITTVFSHAQRVVVCAGCSTILC 266
IDLLHP P E+RKHKLKRLV PNS+FMDVKC GC+ ITTVFSH+Q VVVC C T+LC
Sbjct: 7 IDLLHPPPELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVCGNCQTVLC 66
Query: 267 QPTGGRARLTEGCSFRRK 320
QPTGG+ARL EGCSFR+K
Sbjct: 67 QPTGGKARLQEGCSFRKK 84
>At3g61110.1 68416.m06839 40S ribosomal protein S27 (ARS27A)
identical to cDNA ribosomal protein S27 (ARS27A)
GI:4193381
Length = 86
Score = 135 bits (327), Expect = 3e-32
Identities = 59/78 (75%), Positives = 66/78 (84%)
Frame = +3
Query: 87 IDLLHPSPASERRKHKLKRLVPHPNSYFMDVKCPGCYKITTVFSHAQRVVVCAGCSTILC 266
IDLL+P E+RKHKLKRLV PNS+FMDVKC GC+ ITTVFSH+Q VVVC C TILC
Sbjct: 7 IDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVCGNCQTILC 66
Query: 267 QPTGGRARLTEGCSFRRK 320
QPTGG+A+LTEGCSFRRK
Sbjct: 67 QPTGGKAKLTEGCSFRRK 84
>At2g45710.1 68415.m05685 40S ribosomal protein S27 (RPS27A)
Length = 84
Score = 130 bits (313), Expect = 2e-30
Identities = 55/78 (70%), Positives = 65/78 (83%)
Frame = +3
Query: 87 IDLLHPSPASERRKHKLKRLVPHPNSYFMDVKCPGCYKITTVFSHAQRVVVCAGCSTILC 266
IDLL+P E+RKHKLKRLV PNS+FMDVKC GC+ ITTVFSH+Q VV+C C T+LC
Sbjct: 7 IDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVMCGNCQTLLC 66
Query: 267 QPTGGRARLTEGCSFRRK 320
PTGG+A+LTEGCSFR+K
Sbjct: 67 TPTGGKAKLTEGCSFRKK 84
>At1g59910.1 68414.m06749 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02128
Length = 929
Score = 31.9 bits (69), Expect = 0.60
Identities = 13/30 (43%), Positives = 13/30 (43%)
Frame = +3
Query: 570 PXXPPXPXXGGXXPPPKKKKXWXXXXPPPP 659
P PP P PPP KK PPPP
Sbjct: 384 PPPPPPPSAAAPPPPPPPKKGPAAPPPPPP 413
Score = 30.3 bits (65), Expect = 1.8
Identities = 15/39 (38%), Positives = 15/39 (38%)
Frame = +3
Query: 570 PXXPPXPXXGGXXPPPKKKKXWXXXXPPPPXIPRXKXXP 686
P PP P G PPP PPPP P K P
Sbjct: 395 PPPPPPPKKGPAAPPPPPPPGKKGAGPPPPP-PMSKKGP 432
Score = 27.9 bits (59), Expect = 9.7
Identities = 12/30 (40%), Positives = 12/30 (40%)
Frame = +3
Query: 570 PXXPPXPXXGGXXPPPKKKKXWXXXXPPPP 659
P PP PPP KK PPPP
Sbjct: 385 PPPPPPSAAAPPPPPPPKKGPAAPPPPPPP 414
>At4g21100.1 68417.m03051 UV-damaged DNA-binding protein, putative
similar to UV-damaged DNA binding protein (GI:12082087)
[Oryza sativa] and damage-specific DNA binding protein
1, Homo sapiens, PIR2:I38908; contains Pfam PF03178 :
CPSF A subunit region
Length = 1088
Score = 31.5 bits (68), Expect = 0.79
Identities = 24/86 (27%), Positives = 39/86 (45%), Gaps = 8/86 (9%)
Frame = +3
Query: 51 RLCIKAVTMPLAIDLLHPSPASERRKHKLKRLVPHPN-----SYFMDVKCPGCYKITTVF 215
R+C + T AI L P++E + RL+ + SY +D GC ++ F
Sbjct: 717 RICHQEQTRTFAISCLRNEPSAEESESHFVRLLDAQSFEFLSSYPLDAFECGCSILSCSF 776
Query: 216 SHAQRVVVCAGCSTIL---CQPTGGR 284
+ + V C G + +L +PT GR
Sbjct: 777 TDDKNVYYCVGTAYVLPEENEPTKGR 802
>At5g62640.1 68418.m07862 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965
Length = 520
Score = 30.3 bits (65), Expect = 1.8
Identities = 14/39 (35%), Positives = 18/39 (46%)
Frame = +3
Query: 570 PXXPPXPXXGGXXPPPKKKKXWXXXXPPPPXIPRXKXXP 686
P PP P PPPK++ PPPP +P+ P
Sbjct: 217 PFPPPPPG-----PPPKEQDFVRPPLPPPPQLPQSSQPP 250
>At5g54650.2 68418.m06805 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 900
Score = 30.3 bits (65), Expect = 1.8
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = +1
Query: 571 PXPXPPPXXGXXXPPQKKKXXGXXXPPP 654
P P PPP G PP G PPP
Sbjct: 389 PPPAPPPGSGGPKPPPPPGPKGPRPPPP 416
Score = 27.9 bits (59), Expect = 9.7
Identities = 11/27 (40%), Positives = 12/27 (44%)
Frame = +3
Query: 579 PPXPXXGGXXPPPKKKKXWXXXXPPPP 659
PP P PPP+K PPPP
Sbjct: 160 PPRPPTRPKSPPPRKSSFPPSRSPPPP 186
>At5g54650.1 68418.m06804 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 900
Score = 30.3 bits (65), Expect = 1.8
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = +1
Query: 571 PXPXPPPXXGXXXPPQKKKXXGXXXPPP 654
P P PPP G PP G PPP
Sbjct: 389 PPPAPPPGSGGPKPPPPPGPKGPRPPPP 416
Score = 27.9 bits (59), Expect = 9.7
Identities = 11/27 (40%), Positives = 12/27 (44%)
Frame = +3
Query: 579 PPXPXXGGXXPPPKKKKXWXXXXPPPP 659
PP P PPP+K PPPP
Sbjct: 160 PPRPPTRPKSPPPRKSSFPPSRSPPPP 186
>At2g44870.1 68415.m05586 expressed protein
Length = 248
Score = 30.3 bits (65), Expect = 1.8
Identities = 11/40 (27%), Positives = 21/40 (52%)
Frame = +3
Query: 159 NSYFMDVKCPGCYKITTVFSHAQRVVVCAGCSTILCQPTG 278
N++ + +CP C + + +++ C GC I+ QP G
Sbjct: 175 NNFVIKGECPACKR--QFIGYKNQIIRCEGCGNIVWQPQG 212
>At1g61080.1 68414.m06877 proline-rich family protein
Length = 907
Score = 30.3 bits (65), Expect = 1.8
Identities = 13/39 (33%), Positives = 13/39 (33%)
Frame = +3
Query: 570 PXXPPXPXXGGXXPPPKKKKXWXXXXPPPPXIPRXKXXP 686
P PP P PPP PPPP P P
Sbjct: 537 PPPPPPPGTAAAPPPPPPPPGTQAAPPPPPPPPMQNRAP 575
Score = 29.1 bits (62), Expect = 4.2
Identities = 12/33 (36%), Positives = 12/33 (36%)
Frame = +3
Query: 570 PXXPPXPXXGGXXPPPKKKKXWXXXXPPPPXIP 668
P PP P PPP PPPP P
Sbjct: 523 PPPPPPPPRAAVAPPPPPPPPGTAAAPPPPPPP 555
Score = 29.1 bits (62), Expect = 4.2
Identities = 13/39 (33%), Positives = 14/39 (35%)
Frame = +3
Query: 570 PXXPPXPXXGGXXPPPKKKKXWXXXXPPPPXIPRXKXXP 686
P PP P PPP PPPP P + P
Sbjct: 524 PPPPPPPRAAVAPPPPPPPPGTAAAPPPPPPPPGTQAAP 562
>At4g13340.1 68417.m02084 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 760
Score = 29.9 bits (64), Expect = 2.4
Identities = 12/33 (36%), Positives = 13/33 (39%)
Frame = +3
Query: 570 PXXPPXPXXGGXXPPPKKKKXWXXXXPPPPXIP 668
P PP P PPP + PPPP P
Sbjct: 428 PPSPPPPVYSPPPPPPPPPPVYSPPPPPPPPPP 460
Score = 27.9 bits (59), Expect = 9.7
Identities = 16/65 (24%), Positives = 16/65 (24%)
Frame = +2
Query: 530 PPXXXXPXXXXXKXPXPXXPXXGGXXXPPKKKKXLXXXXPPPPXNPAXXXXPFXXXXXXX 709
PP P P P P PP PPPP P P
Sbjct: 427 PPPSPPPPVYSPPPPPPPPPPVYSPPPPPPPPPPPPVYSPPPPPPPPPPPPPVYSPPPPS 486
Query: 710 PXXXP 724
P P
Sbjct: 487 PPPPP 491
Score = 27.9 bits (59), Expect = 9.7
Identities = 11/29 (37%), Positives = 11/29 (37%)
Frame = +1
Query: 571 PXPXPPPXXGXXXPPQKKKXXGXXXPPPP 657
P P PPP PP PPPP
Sbjct: 487 PPPPPPPVYSPPPPPPPPPPPPVYSPPPP 515
>At1g31810.1 68414.m03904 formin homology 2 domain-containing
protein / FH2 domain-containing protein low similarity
to SP|P48608 Diaphanous protein {Drosophila
melanogaster}; contains Pfam profile PF02181: Formin
Homology 2(FH2) Domain
Length = 1201
Score = 29.9 bits (64), Expect = 2.4
Identities = 13/39 (33%), Positives = 15/39 (38%)
Frame = +3
Query: 570 PXXPPXPXXGGXXPPPKKKKXWXXXXPPPPXIPRXKXXP 686
P PP P PPP + PPPP P + P
Sbjct: 575 PPPPPPPLPSRSIPPPLAQPPPPRPPPPPPPPPSSRSIP 613
Score = 27.9 bits (59), Expect = 9.7
Identities = 12/33 (36%), Positives = 12/33 (36%)
Frame = +1
Query: 571 PXPXPPPXXGXXXPPQKKKXXGXXXPPPPXXSR 669
P P PPP PP PPPP R
Sbjct: 715 PPPPPPPLSKTPAPPPPPLSKTPVPPPPPGLGR 747
>At3g22800.1 68416.m02874 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycsimilar to extensin-like protein [Lycopersicon
esculentum] gi|5917664|gb|AAD55979; contains
leucine-rich repeats, Pfam:PF00560; contains proline
rich extensin domains, INTERPRO:IPR002965
Length = 470
Score = 28.7 bits (61), Expect = 5.6
Identities = 12/33 (36%), Positives = 13/33 (39%)
Frame = +3
Query: 570 PXXPPXPXXGGXXPPPKKKKXWXXXXPPPPXIP 668
P PP P PPP + PPPP P
Sbjct: 389 PPPPPPPPPPPPPPPPPPPYVYPSPPPPPPSPP 421
Score = 27.9 bits (59), Expect = 9.7
Identities = 11/30 (36%), Positives = 12/30 (40%)
Frame = +3
Query: 570 PXXPPXPXXGGXXPPPKKKKXWXXXXPPPP 659
P PP P PPP + PPPP
Sbjct: 387 PPPPPPPPPPPPPPPPPPPPPYVYPSPPPP 416
>At2g33845.1 68415.m04154 DNA-binding protein-related contains weak
similarity to G-quartet DNA binding protein 3
[Tetrahymena thermophila] gi|4583503|gb|AAD25098
Length = 182
Score = 28.7 bits (61), Expect = 5.6
Identities = 13/40 (32%), Positives = 14/40 (35%)
Frame = +3
Query: 579 PPXPXXGGXXPPPKKKKXWXXXXPPPPXIPRXKXXPXXXK 698
PP P G PPP PP P+ K P K
Sbjct: 20 PPPPRRQGRNPPPPSSSTRTSQNQPPQHTPQEKKKPVFVK 59
>At1g49750.1 68414.m05579 leucine-rich repeat family protein
contains leucine-rich repeats, Pfam:PF00560
Length = 494
Score = 28.7 bits (61), Expect = 5.6
Identities = 13/39 (33%), Positives = 14/39 (35%)
Frame = +3
Query: 570 PXXPPXPXXGGXXPPPKKKKXWXXXXPPPPXIPRXKXXP 686
P PP P PPP PPPP +P P
Sbjct: 63 PPPPPPPCPPPPSPPPCPPPPSPPPSPPPPQLPPPPQLP 101
>At4g18570.1 68417.m02749 proline-rich family protein common family
members: At3g25690, At4g04980, At5g61090 [Arabidopsis
thaliana]
Length = 642
Score = 28.3 bits (60), Expect = 7.3
Identities = 12/30 (40%), Positives = 12/30 (40%)
Frame = +3
Query: 570 PXXPPXPXXGGXXPPPKKKKXWXXXXPPPP 659
P PP P PPP K PPPP
Sbjct: 313 PPPPPPPLLQQPPPPPSVSKAPPPPPPPPP 342
>At3g25690.1 68416.m03197 hydroxyproline-rich glycoprotein family
protein Common family members: At4g18570, At4g04980,
At5g61090 [Arabidopsis thaliana]; identical to cDNA
CHUP1 for actin binding protein GI:28071264
Length = 1004
Score = 28.3 bits (60), Expect = 7.3
Identities = 14/33 (42%), Positives = 14/33 (42%)
Frame = +3
Query: 570 PXXPPXPXXGGXXPPPKKKKXWXXXXPPPPXIP 668
P PP P GG PPP PPPP P
Sbjct: 679 PPPPPPPPGGGPPPPP------GGGPPPPPPPP 705
>At5g07780.1 68418.m00890 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 464
Score = 27.9 bits (59), Expect = 9.7
Identities = 15/46 (32%), Positives = 17/46 (36%), Gaps = 3/46 (6%)
Frame = +3
Query: 570 PXXPPXPXX---GGXXPPPKKKKXWXXXXPPPPXIPRXKXXPXXXK 698
P PP P PPP + PPPP +PR P K
Sbjct: 30 PPPPPPPLMRRRAPPPPPPPLMRRRAPPPPPPPPLPRPCSRPPKTK 75
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,085,509
Number of Sequences: 28952
Number of extensions: 257111
Number of successful extensions: 1619
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 628
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1165
length of database: 12,070,560
effective HSP length: 81
effective length of database: 9,725,448
effective search space used: 2120147664
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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