Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_E24
(837 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g45130.1 68414.m05173 beta-galactosidase, putative / lactase,... 69 3e-12
At2g28470.1 68415.m03460 beta-galactosidase, putative / lactase,... 69 4e-12
At5g20710.1 68418.m02459 beta-galactosidase, putative / lactase,... 66 4e-11
At4g36360.2 68417.m05164 beta-galactosidase, putative / lactase,... 65 6e-11
At4g36360.1 68417.m05163 beta-galactosidase, putative / lactase,... 65 6e-11
At2g32810.1 68415.m04016 beta-galactosidase, putative / lactase,... 62 3e-10
At4g38590.1 68417.m05462 glycosyl hydrolase family 35 protein si... 62 6e-10
At2g16730.1 68415.m01919 glycosyl hydrolase family 35 protein si... 61 8e-10
At1g72990.1 68414.m08441 glycosyl hydrolase family 35 protein si... 61 8e-10
At4g26140.2 68417.m03763 beta-galactosidase, putative / lactase,... 61 1e-09
At4g26140.1 68417.m03762 beta-galactosidase, putative / lactase,... 61 1e-09
At3g13750.1 68416.m01735 beta-galactosidase, putative / lactase,... 61 1e-09
At1g77410.1 68414.m09015 beta-galactosidase, putative / lactase,... 61 1e-09
At1g31740.1 68414.m03894 beta-galactosidase, putative / lactase,... 61 1e-09
At4g35010.1 68417.m04965 glycosyl hydrolase family 35 protein si... 60 2e-09
At5g63800.1 68418.m08007 glycosyl hydrolase family 35 protein si... 58 7e-09
At5g56870.1 68418.m07097 beta-galactosidase, putative / lactase,... 56 4e-08
At5g63810.1 68418.m08008 beta-galactosidase, putative / lactase,... 53 2e-07
At3g52840.1 68416.m05823 beta-galactosidase, putative / lactase,... 53 2e-07
At5g63420.1 68418.m07962 metallo-beta-lactamase family protein 31 1.3
At1g16900.1 68414.m02047 curculin-like (mannose-binding) lectin ... 29 3.8
At1g58190.1 68414.m06605 leucine-rich repeat family protein cont... 29 5.1
At2g32800.1 68415.m04015 protein kinase family protein contains ... 28 6.7
At1g58220.1 68414.m06612 myb family transcription factor contain... 28 8.8
>At1g45130.1 68414.m05173 beta-galactosidase, putative / lactase,
putative similar to beta-galactosidase [Lycopersicon
esculentum] GI:7939619, beta-galactosidase BG1
GI:15081596 from [Vitis vinifera]; contains Pfam profile
PF01301: Glycosyl hydrolases family 35
Length = 732
Score = 69.3 bits (162), Expect = 3e-12
Identities = 31/59 (52%), Positives = 39/59 (66%)
Frame = +1
Query: 529 WRDRLHKFKAAGLNTVATYVEWSYHEPXEKQYNFEGDRDLVRFVQTAAEVXLHVLLRVG 705
W D + K K GL+ + TYV W+ HEP YNFEG DLVRF++T EV L+V LR+G
Sbjct: 62 WEDLIKKAKDGGLDVIDTYVFWNGHEPSPGTYNFEGRYDLVRFIKTIQEVGLYVHLRIG 120
>At2g28470.1 68415.m03460 beta-galactosidase, putative / lactase,
putative similar to Beta-galactosidase precursor
SP:P48980 from [Lycopersicon esculentum]
Length = 852
Score = 68.9 bits (161), Expect = 4e-12
Identities = 30/59 (50%), Positives = 41/59 (69%)
Frame = +1
Query: 529 WRDRLHKFKAAGLNTVATYVEWSYHEPXEKQYNFEGDRDLVRFVQTAAEVXLHVLLRVG 705
W + + K K GL+ + TYV WS HEP + +YNFEG DLV+FV+ AA+ L+V LR+G
Sbjct: 63 WPELIQKSKDGGLDVIETYVFWSGHEPEKNKYNFEGRYDLVKFVKLAAKAGLYVHLRIG 121
>At5g20710.1 68418.m02459 beta-galactosidase, putative / lactase,
putative strong similarity to beta-galactosidase
precursor (EC 3.2.1.23) (Lactase) SP:P49676 from
[Brassica oleracea]
Length = 769
Score = 65.7 bits (153), Expect = 4e-11
Identities = 25/59 (42%), Positives = 41/59 (69%)
Frame = +1
Query: 529 WRDRLHKFKAAGLNTVATYVEWSYHEPXEKQYNFEGDRDLVRFVQTAAEVXLHVLLRVG 705
W D ++K K GL+ + TYV W+ HEP ++Y+F G+ D+VRF++T + L+ +LR+G
Sbjct: 2 WPDLINKAKDGGLDAIETYVFWNAHEPKRREYDFSGNLDVVRFIKTIQDAGLYSVLRIG 60
>At4g36360.2 68417.m05164 beta-galactosidase, putative / lactase,
putative similar to beta-galactosidase precursor
SP:P48980 from [Lycopersicon esculentum]
Length = 855
Score = 64.9 bits (151), Expect = 6e-11
Identities = 27/59 (45%), Positives = 38/59 (64%)
Frame = +1
Query: 529 WRDRLHKFKAAGLNTVATYVEWSYHEPXEKQYNFEGDRDLVRFVQTAAEVXLHVLLRVG 705
W D + K K G++ + TYV W+ HEP +Y+FEG DLVRFV+T + L+ LR+G
Sbjct: 64 WEDLIQKAKDGGIDVIETYVFWNLHEPSPGKYDFEGRNDLVRFVKTIHKAGLYAHLRIG 122
>At4g36360.1 68417.m05163 beta-galactosidase, putative / lactase,
putative similar to beta-galactosidase precursor
SP:P48980 from [Lycopersicon esculentum]
Length = 856
Score = 64.9 bits (151), Expect = 6e-11
Identities = 27/59 (45%), Positives = 38/59 (64%)
Frame = +1
Query: 529 WRDRLHKFKAAGLNTVATYVEWSYHEPXEKQYNFEGDRDLVRFVQTAAEVXLHVLLRVG 705
W D + K K G++ + TYV W+ HEP +Y+FEG DLVRFV+T + L+ LR+G
Sbjct: 64 WEDLIQKAKDGGIDVIETYVFWNLHEPSPGKYDFEGRNDLVRFVKTIHKAGLYAHLRIG 122
>At2g32810.1 68415.m04016 beta-galactosidase, putative / lactase,
putative similar to beta-galactosidase GI:7939617 from
[Lycopersicon esculentum]
Length = 887
Score = 62.5 bits (145), Expect = 3e-10
Identities = 28/59 (47%), Positives = 37/59 (62%)
Frame = +1
Query: 529 WRDRLHKFKAAGLNTVATYVEWSYHEPXEKQYNFEGDRDLVRFVQTAAEVXLHVLLRVG 705
W D + K K G + V TYV W+ HEP + QYNFEG DLV+FV+ L++ LR+G
Sbjct: 69 WSDLIAKSKEGGADVVQTYVFWNGHEPVKGQYNFEGRYDLVKFVKLIGSSGLYLHLRIG 127
>At4g38590.1 68417.m05462 glycosyl hydrolase family 35 protein
similar to beta-galactosidase GI:7939621 from
[Lycopersicon esculentum]; contains Pfam profile PF01301
: Glycosyl hydrolases family 35
Length = 988
Score = 61.7 bits (143), Expect = 6e-10
Identities = 26/59 (44%), Positives = 39/59 (66%)
Frame = +1
Query: 529 WRDRLHKFKAAGLNTVATYVEWSYHEPXEKQYNFEGDRDLVRFVQTAAEVXLHVLLRVG 705
W + K + GLNT+ TYV W+ HEP + +Y+F+G DLV+F++ E L+V LR+G
Sbjct: 2 WPSIIDKARIGGLNTIQTYVFWNVHEPEQGKYDFKGRFDLVKFIKLIHEKGLYVTLRLG 60
>At2g16730.1 68415.m01919 glycosyl hydrolase family 35 protein
similar to beta-galactosidase BG1 GI:15081596 from
[Vitis vinifera]
Length = 848
Score = 61.3 bits (142), Expect = 8e-10
Identities = 25/59 (42%), Positives = 39/59 (66%)
Frame = +1
Query: 529 WRDRLHKFKAAGLNTVATYVEWSYHEPXEKQYNFEGDRDLVRFVQTAAEVXLHVLLRVG 705
W + + + K GLNT+ TYV W+ HEP + ++NF G DLV+F++ + L+V LR+G
Sbjct: 75 WPNIIKRAKQGGLNTIQTYVFWNVHEPEQGKFNFSGRADLVKFIKLIEKNGLYVTLRLG 133
>At1g72990.1 68414.m08441 glycosyl hydrolase family 35 protein
similar to beta-galactosidase GI:2289790 from [Bacillus
circulans]; contains Pfam profile PF01301: Glycosyl
hydrolases family 35
Length = 697
Score = 61.3 bits (142), Expect = 8e-10
Identities = 29/68 (42%), Positives = 40/68 (58%)
Frame = +1
Query: 517 LAVYWRDRLHKFKAAGLNTVATYVEWSYHEPXEKQYNFEGDRDLVRFVQTAAEVXLHVLL 696
L YW DRL + A GLNT+ YV W+ HEP + FEG DLV F++ ++ V+L
Sbjct: 90 LPEYWEDRLLRANALGLNTIQVYVPWNLHEPKPGKMVFEGIGDLVSFLKLCEKLDFLVML 149
Query: 697 RVGSLHLC 720
R G ++C
Sbjct: 150 RAGP-YIC 156
Score = 34.3 bits (75), Expect = 0.10
Identities = 37/139 (26%), Positives = 54/139 (38%), Gaps = 3/139 (2%)
Frame = +2
Query: 377 TNEDHQNKLSNEFKMKGHNISIVGDKFMIDGKPLHIISGSLHYFRG-PWQCIGVTDCISS 553
T + + + + KM I D F DG II G LHYFR P ++
Sbjct: 44 TPQSLPSAIPQDEKMISRKFYIKDDNFWKDGNRFQIIGGDLHYFRVLPEYWEDRLLRANA 103
Query: 554 KPLVSTQLLRT*NGVTMSPRRNSIISKEIVTW*DLFRQRPKXACTFY*GLGPYICAERDL 733
L + Q+ N P+ ++ + I + K GPYIC E DL
Sbjct: 104 LGLNTIQVYVPWN--LHEPKPGKMVFEGIGDLVSFLKLCEKLDFLVMLRAGPYICGEWDL 161
Query: 734 GXLP--VLATRKVSEYKTS 784
G P +LA + + +TS
Sbjct: 162 GGFPAWLLAVKPRLQLRTS 180
>At4g26140.2 68417.m03763 beta-galactosidase, putative / lactase,
putative similar to beta-galactosidase precursor
GI:3869280 from [Carica papaya]
Length = 636
Score = 60.9 bits (141), Expect = 1e-09
Identities = 35/89 (39%), Positives = 48/89 (53%), Gaps = 6/89 (6%)
Frame = +1
Query: 457 YDRREASTHHIRVIALLQGSLAV------YWRDRLHKFKAAGLNTVATYVEWSYHEPXEK 618
YDR+ + R I LL GS+ W D + K K GL+ + TYV W+ HEP
Sbjct: 31 YDRKAVIINGQRRI-LLSGSIHYPRSTPEMWPDLIQKAKDGGLDVIQTYVFWNGHEPSPG 89
Query: 619 QYNFEGDRDLVRFVQTAAEVXLHVLLRVG 705
QY FE DLV+F++ + L+V LR+G
Sbjct: 90 QYYFEDRYDLVKFIKVVQQAGLYVHLRIG 118
>At4g26140.1 68417.m03762 beta-galactosidase, putative / lactase,
putative similar to beta-galactosidase precursor
GI:3869280 from [Carica papaya]
Length = 728
Score = 60.9 bits (141), Expect = 1e-09
Identities = 35/89 (39%), Positives = 48/89 (53%), Gaps = 6/89 (6%)
Frame = +1
Query: 457 YDRREASTHHIRVIALLQGSLAV------YWRDRLHKFKAAGLNTVATYVEWSYHEPXEK 618
YDR+ + R I LL GS+ W D + K K GL+ + TYV W+ HEP
Sbjct: 31 YDRKAVIINGQRRI-LLSGSIHYPRSTPEMWPDLIQKAKDGGLDVIQTYVFWNGHEPSPG 89
Query: 619 QYNFEGDRDLVRFVQTAAEVXLHVLLRVG 705
QY FE DLV+F++ + L+V LR+G
Sbjct: 90 QYYFEDRYDLVKFIKVVQQAGLYVHLRIG 118
>At3g13750.1 68416.m01735 beta-galactosidase, putative / lactase,
putative similar to beta-galactosidase precursor
SP:P48980 from [Lycopersicon esculentum]
Length = 847
Score = 60.9 bits (141), Expect = 1e-09
Identities = 26/59 (44%), Positives = 38/59 (64%)
Frame = +1
Query: 529 WRDRLHKFKAAGLNTVATYVEWSYHEPXEKQYNFEGDRDLVRFVQTAAEVXLHVLLRVG 705
W D + K K GL+ + TYV W+ HEP +Y FEG+ DLV+FV+ + L++ LR+G
Sbjct: 65 WPDLIRKAKEGGLDVIQTYVFWNGHEPSPGKYYFEGNYDLVKFVKLVQQSGLYLHLRIG 123
>At1g77410.1 68414.m09015 beta-galactosidase, putative / lactase,
putative similar to beta-galactosidase SP:P45582 from
[Asparagus officinalis]
Length = 815
Score = 60.9 bits (141), Expect = 1e-09
Identities = 24/59 (40%), Positives = 38/59 (64%)
Frame = +1
Query: 529 WRDRLHKFKAAGLNTVATYVEWSYHEPXEKQYNFEGDRDLVRFVQTAAEVXLHVLLRVG 705
W + K K+ G++ V TYV W+ HEP + Q++F G RD+V+F++ L+V LR+G
Sbjct: 56 WPSLIAKAKSGGIDVVDTYVFWNVHEPQQGQFDFSGSRDIVKFIKEVKNHGLYVCLRIG 114
>At1g31740.1 68414.m03894 beta-galactosidase, putative / lactase,
putative similar to beta-galactosidase precursor
[Brassica oleracea] SWISS-PROT:P49676
Length = 786
Score = 60.9 bits (141), Expect = 1e-09
Identities = 24/59 (40%), Positives = 38/59 (64%)
Frame = +1
Query: 529 WRDRLHKFKAAGLNTVATYVEWSYHEPXEKQYNFEGDRDLVRFVQTAAEVXLHVLLRVG 705
W D + K K L+ + TYV W+ HEP +QY+F G+ DL+RF++T ++ +LR+G
Sbjct: 76 WPDLIKKGKEGSLDAIETYVFWNAHEPTRRQYDFSGNLDLIRFLKTIQNEGMYGVLRIG 134
>At4g35010.1 68417.m04965 glycosyl hydrolase family 35 protein
similar to beta-galactosidase BG1 GI:15081596 from
[Vitis vinifera]
Length = 845
Score = 60.1 bits (139), Expect = 2e-09
Identities = 24/59 (40%), Positives = 38/59 (64%)
Frame = +1
Query: 529 WRDRLHKFKAAGLNTVATYVEWSYHEPXEKQYNFEGDRDLVRFVQTAAEVXLHVLLRVG 705
W + + K GLNT+ TYV W+ HEP + ++NF G DLV+F++ + ++V LR+G
Sbjct: 72 WPSIIKRAKQGGLNTIQTYVFWNVHEPQQGKFNFSGRADLVKFIKLIQKNGMYVTLRLG 130
>At5g63800.1 68418.m08007 glycosyl hydrolase family 35 protein
similar to beta-galactosidase GI:7939621 from
[Lycopersicon esculentum]; contains Pfam profile
PF01301: Glycosyl hydrolases family 35
Length = 718
Score = 58.0 bits (134), Expect = 7e-09
Identities = 24/59 (40%), Positives = 35/59 (59%)
Frame = +1
Query: 529 WRDRLHKFKAAGLNTVATYVEWSYHEPXEKQYNFEGDRDLVRFVQTAAEVXLHVLLRVG 705
W + K K G++ + TYV W+ HEP QY+F G DLV+F++ L+V LR+G
Sbjct: 63 WPSLIKKTKEGGIDVIQTYVFWNLHEPKLGQYDFSGRNDLVKFIKEIRSQGLYVCLRIG 121
>At5g56870.1 68418.m07097 beta-galactosidase, putative / lactase,
putative similar to beta-galactosidase precursor
GI:3869280 from [Carica papaya]
Length = 724
Score = 55.6 bits (128), Expect = 4e-08
Identities = 36/90 (40%), Positives = 49/90 (54%), Gaps = 7/90 (7%)
Frame = +1
Query: 457 YDRREASTHHIRVIALLQGSLAV------YWRDRLHKFKAAGLNTVATYVEWSYHEPXEK 618
YDR+ + R I LL GS+ W + K K GL+ + TYV W+ HEP
Sbjct: 31 YDRKAVIINGQRRI-LLSGSIHYPRSTPEMWPGLIQKAKEGGLDVIETYVFWNGHEPSPG 89
Query: 619 QYNFEGDR-DLVRFVQTAAEVXLHVLLRVG 705
QY F GDR DLV+F++ + L+V LR+G
Sbjct: 90 QYYF-GDRYDLVKFIKLVHQAGLYVNLRIG 118
>At5g63810.1 68418.m08008 beta-galactosidase, putative / lactase,
putative similar to beta-galactosidase GI:7939621 from
[Lycopersicon esculentum]; contains Pfam profile
PF01301: Glycosyl hydrolases family 35
Length = 741
Score = 53.2 bits (122), Expect = 2e-07
Identities = 25/102 (24%), Positives = 50/102 (49%)
Frame = +1
Query: 400 TFKRVQDERTQHKHRWRQVYDRREASTHHIRVIALLQGSLAVYWRDRLHKFKAAGLNTVA 579
+++ ++ + HR + +RR+ I S+ W + K G N +
Sbjct: 23 SWRSIEAANVSYDHRSLTIGNRRQLI---ISAAIHYPRSVPAMWPSLVQTAKEGGCNAIE 79
Query: 580 TYVEWSYHEPXEKQYNFEGDRDLVRFVQTAAEVXLHVLLRVG 705
+YV W+ HEP +Y F G ++V+F++ + +H++LR+G
Sbjct: 80 SYVFWNGHEPSPGKYYFGGRYNIVKFIKIVQQAGMHMILRIG 121
>At3g52840.1 68416.m05823 beta-galactosidase, putative / lactase,
putative similar to beta-galactosidase precursor
GI:3869280 from [Carica papaya]
Length = 727
Score = 53.2 bits (122), Expect = 2e-07
Identities = 23/59 (38%), Positives = 34/59 (57%)
Frame = +1
Query: 529 WRDRLHKFKAAGLNTVATYVEWSYHEPXEKQYNFEGDRDLVRFVQTAAEVXLHVLLRVG 705
W D + K K GL+ + TYV W+ HEP Y F+ DLV+F + + L++ LR+G
Sbjct: 60 WPDLIKKAKEGGLDVIQTYVFWNGHEPSPGNYYFQDRYDLVKFTKLVHQAGLYLDLRIG 118
>At5g63420.1 68418.m07962 metallo-beta-lactamase family protein
Length = 911
Score = 30.7 bits (66), Expect = 1.3
Identities = 24/108 (22%), Positives = 45/108 (41%), Gaps = 1/108 (0%)
Frame = +2
Query: 203 SGSRQFTAAPLSGQRGSSSCVEELIFFKMTLLIATFALLCFLGQTYSLEDYAPVVIRVTN 382
+G + APL G+ +EEL +TL+++ + G+T S + A ++R
Sbjct: 265 TGDWKIDEAPLDGKVFDREALEELSKEGVTLMMSDSTNVLSPGRTISEKVVADALVRNVM 324
Query: 383 EDHQNKLSNEFKMKGHNISIVGDKFMIDGKPLHIISGSLH-YFRGPWQ 523
++ +F H + + I G+ L + SL Y W+
Sbjct: 325 AAKGRVITTQFASNIHRLGSIKAAADITGRKLVFVGMSLRTYLEAAWR 372
>At1g16900.1 68414.m02047 curculin-like (mannose-binding) lectin
family protein very low similarity to Ser Thr protein
kinase GI:2598067 from (Zea mays); contains Pfam lectin
(probable mannose binding) domain PF01453
Length = 919
Score = 29.1 bits (62), Expect = 3.8
Identities = 24/82 (29%), Positives = 37/82 (45%), Gaps = 1/82 (1%)
Frame = +2
Query: 128 VSKIRECSRVLITYLLVE-ALASDCFSGSRQFTAAPLSGQRGSSSCVEELIFFKMTLLIA 304
V+ R+ + + TY + L S CF S F + S S S L+F K + +A
Sbjct: 496 VALSRKYGKRIATYAVAMLCLTSGCFFASTSFLPSSFSMYAISLSS-GLLLFEKYAMAVA 554
Query: 305 TFALLCFLGQTYSLEDYAPVVI 370
+ LG +S+ + PVVI
Sbjct: 555 VSVVGVILGWPFSILAFLPVVI 576
>At1g58190.1 68414.m06605 leucine-rich repeat family protein contains
leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Cf-2.2
[Lycopersicon pimpinellifolium] gi|1184077|gb|AAC15780
Length = 1784
Score = 28.7 bits (61), Expect = 5.1
Identities = 9/13 (69%), Positives = 12/13 (92%)
Frame = +3
Query: 744 PYWLLGKYPNIKL 782
PYWLL KYPN+++
Sbjct: 1216 PYWLLEKYPNLRV 1228
>At2g32800.1 68415.m04015 protein kinase family protein contains
dual protein kinase domains, Pfam:PF00069
Length = 851
Score = 28.3 bits (60), Expect = 6.7
Identities = 16/47 (34%), Positives = 26/47 (55%)
Frame = +2
Query: 317 LCFLGQTYSLEDYAPVVIRVTNEDHQNKLSNEFKMKGHNISIVGDKF 457
LCF+ T S E PV++ T E+ + K S + + +S++GD F
Sbjct: 9 LCFVLPTESGELKPPVMVEETTEEEEEKKSRDCGRQ--VVSLIGDLF 53
>At1g58220.1 68414.m06612 myb family transcription factor contains
Pfam profile: PF00249: Myb-like DNA-binding domain
Length = 834
Score = 27.9 bits (59), Expect = 8.8
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = -1
Query: 249 PR*PDNGAAVNCLDPEKQSLASAST 175
P+ P GAA N + P+K S+ SA+T
Sbjct: 687 PQKPSVGAAANVVTPQKPSVGSAAT 711
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,968,974
Number of Sequences: 28952
Number of extensions: 342880
Number of successful extensions: 824
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 777
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 821
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1931371200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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