Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP03_F_E17
         (880 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2 (e...    50   2e-06
At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1 (e...    46   3e-05
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF...    45   8e-05
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id...    45   8e-05
At2g40660.1 68415.m05017 tRNA-binding region domain-containing p...    31   1.0  
At3g51550.1 68416.m05645 protein kinase family protein contains ...    28   9.5  

>At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2
           (eEF1Balpha2) identical to elongation factor 1B
           alpha-subunit [Arabidopsis thaliana] GI:6686821
          Length = 224

 Score = 50.0 bits (114), Expect = 2e-06
 Identities = 21/34 (61%), Positives = 27/34 (79%)
 Frame = +2

Query: 482 ADXKSKKPALIXKSSILLDVKPWDDETDMKEMEK 583
           A   +KKP    KSS+L+DVKPWDDETDMK++E+
Sbjct: 123 AKKDTKKPKESGKSSVLMDVKPWDDETDMKKLEE 156



 Score = 37.9 bits (84), Expect = 0.009
 Identities = 17/45 (37%), Positives = 27/45 (60%)
 Frame = +1

Query: 160 KYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIAS 294
           ++LA K+Y+SG   S  DV+V+  V   P+   P+  +WY  +AS
Sbjct: 19  EHLAGKTYISGDQLSVDDVKVYAAVPVKPSDAFPNASKWYESVAS 63



 Score = 34.3 bits (75), Expect = 0.11
 Identities = 16/21 (76%), Positives = 16/21 (76%)
 Frame = +1

Query: 622 GASKLVPVGYGINKLXXMCVI 684
           GASKLVPVGYGI KL  M  I
Sbjct: 169 GASKLVPVGYGIKKLTIMFTI 189


>At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1
           (eEF1Balpha1) identical to elongation factor 1B
           alpha-subunit [Arabidopsis thaliana] GI:6686819
          Length = 228

 Score = 46.0 bits (104), Expect = 3e-05
 Identities = 20/34 (58%), Positives = 26/34 (76%)
 Frame = +2

Query: 482 ADXKSKKPALIXKSSILLDVKPWDDETDMKEMEK 583
           A   +KK     KSS+LL+VKPWDDETDMK++E+
Sbjct: 127 AKKDTKKTKESGKSSVLLEVKPWDDETDMKKLEE 160



 Score = 39.5 bits (88), Expect = 0.003
 Identities = 17/46 (36%), Positives = 29/46 (63%)
 Frame = +1

Query: 160 KYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIASY 297
           ++LA K+Y+SG   S  DV+V+  V + P    P+  +WY+ +AS+
Sbjct: 19  EHLAGKTYISGDQLSVDDVKVYAAVLENPGDGFPNASKWYDSVASH 64



 Score = 34.7 bits (76), Expect = 0.082
 Identities = 16/21 (76%), Positives = 16/21 (76%)
 Frame = +1

Query: 622 GASKLVPVGYGINKLXXMCVI 684
           GASKLVPVGYGI KL  M  I
Sbjct: 173 GASKLVPVGYGIKKLTIMMTI 193


>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
           EF-1-beta, putative nearly identical to eEF-1beta
           [Arabidopsis thaliana] GI:398606
          Length = 231

 Score = 44.8 bits (101), Expect = 8e-05
 Identities = 16/22 (72%), Positives = 22/22 (100%)
 Frame = +2

Query: 518 KSSILLDVKPWDDETDMKEMEK 583
           KSS+L+D+KPWDDETDMK++E+
Sbjct: 142 KSSVLMDIKPWDDETDMKKLEE 163



 Score = 42.3 bits (95), Expect = 4e-04
 Identities = 16/43 (37%), Positives = 27/43 (62%)
 Frame = +1

Query: 160 KYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQI 288
           ++L  +SY++GY  S+ D+ VF  + K P +   +V RW+N I
Sbjct: 18  EHLLTRSYITGYQASKDDITVFTALSKPPTSEFVNVSRWFNHI 60



 Score = 38.7 bits (86), Expect = 0.005
 Identities = 17/21 (80%), Positives = 17/21 (80%)
 Frame = +1

Query: 622 GASKLVPVGYGINKLXXMCVI 684
           GASKLVPVGYGI KL  MC I
Sbjct: 176 GASKLVPVGYGIKKLHIMCTI 196


>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
           identical to SP|P48006 Elongation factor 1-beta
           (EF-1-beta) {Arabidopsis thaliana}
          Length = 231

 Score = 44.8 bits (101), Expect = 8e-05
 Identities = 16/22 (72%), Positives = 22/22 (100%)
 Frame = +2

Query: 518 KSSILLDVKPWDDETDMKEMEK 583
           KSS+L+D+KPWDDETDMK++E+
Sbjct: 142 KSSVLIDIKPWDDETDMKKLEE 163



 Score = 41.9 bits (94), Expect = 5e-04
 Identities = 16/43 (37%), Positives = 26/43 (60%)
 Frame = +1

Query: 160 KYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQI 288
           ++L  +SY++GY  S+ D+ VF  + K P +   +  RWYN I
Sbjct: 18  EHLLTRSYITGYQASKDDITVFAALAKPPTSQYVNASRWYNHI 60



 Score = 37.5 bits (83), Expect = 0.012
 Identities = 16/21 (76%), Positives = 17/21 (80%)
 Frame = +1

Query: 622 GASKLVPVGYGINKLXXMCVI 684
           GASKLVPVGYGI KL  +C I
Sbjct: 176 GASKLVPVGYGIKKLQILCTI 196


>At2g40660.1 68415.m05017 tRNA-binding region domain-containing
           protein similar to SP|Q12904 Multisynthetase complex
           auxiliary component p43 [Contains: Endothelial-monocyte
           activating polypeptide II (EMAP-II) (Small inducible
           cytokine subfamily E member 1)] {Homo sapiens}; contains
           Pfam profile PF01588: Putative tRNA binding domain
          Length = 389

 Score = 31.1 bits (67), Expect = 1.0
 Identities = 20/50 (40%), Positives = 26/50 (52%), Gaps = 9/50 (18%)
 Frame = +1

Query: 166 LAEKSYV--SGYTPSQADVQVFEQV-------GKAPAANLPHVLRWYNQI 288
           LA KS +  +G TPS ADV VF  +         +    +PHV+RW N I
Sbjct: 83  LATKSVLLGNGLTPSAADVAVFSALHSSVLGLSDSDKEKVPHVIRWVNYI 132


>At3g51550.1 68416.m05645 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 895

 Score = 27.9 bits (59), Expect = 9.5
 Identities = 11/33 (33%), Positives = 19/33 (57%), Gaps = 1/33 (3%)
 Frame = +2

Query: 248 PLTFPTYYDGIIKLLHTLRLNAKHG-LRGTSPL 343
           P+  P YYD ++  +   ++N   G L GT+P+
Sbjct: 390 PVNKPEYYDSLLNGVEIFKMNTSDGNLAGTNPI 422


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,311,339
Number of Sequences: 28952
Number of extensions: 266044
Number of successful extensions: 645
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 620
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 644
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 2067932800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)