Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_E13
(852 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)... 118 7e-27
At5g42020.2 68418.m05116 luminal binding protein 2 (BiP-2) (BP2)... 116 2e-26
At5g42020.1 68418.m05115 luminal binding protein 2 (BiP-2) (BP2)... 116 2e-26
At5g28540.1 68418.m03480 luminal binding protein 1 (BiP-1) (BP1)... 116 2e-26
At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein, puta... 111 8e-25
At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70... 110 1e-24
At5g02500.1 68418.m00183 heat shock cognate 70 kDa protein 1 (HS... 110 1e-24
At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2 (HS... 110 1e-24
At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3 (HS... 109 2e-24
At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70... 108 5e-24
At4g24280.1 68417.m03486 heat shock protein 70, putative / HSP70... 77 1e-14
At4g37910.1 68417.m05361 heat shock protein 70, mitochondrial, p... 77 2e-14
At5g49910.1 68418.m06180 heat shock protein 70 / HSP70 (HSC70-7)... 74 1e-13
At5g09590.1 68418.m01110 heat shock protein 70 / HSP70 (HSC70-5)... 73 2e-13
At1g79930.1 68414.m09340 heat shock protein, putative contains P... 48 1e-05
At1g79920.2 68414.m09339 heat shock protein 70, putative / HSP70... 48 1e-05
At1g79920.1 68414.m09338 heat shock protein 70, putative / HSP70... 48 1e-05
At1g11660.1 68414.m01339 heat shock protein, putative strong sim... 46 4e-05
At2g32120.2 68415.m03926 heat shock protein 70 family protein / ... 34 0.14
At2g32120.1 68415.m03925 heat shock protein 70 family protein / ... 34 0.14
>At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)
Similar to Arabidopsis luminal binding protein
(gb|D89342); contains Pfam domain PF00012: dnaK protein
Length = 678
Score = 118 bits (283), Expect = 7e-27
Identities = 61/91 (67%), Positives = 67/91 (73%)
Frame = +1
Query: 289 IGTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTT 468
+GTVIGIDLGTTYSCVGVY N VEIIANDQGNRITPS+VAFT D ERLIG+AAKNQ
Sbjct: 49 LGTVIGIDLGTTYSCVGVYHNKHVEIIANDQGNRITPSWVAFT-DTERLIGEAAKNQAAK 107
Query: 469 NPENTVF*CPSV*LVXNXXXQPVXHDVKFLP 561
NPE T+F P + V D+KFLP
Sbjct: 108 NPERTIF-DPKRLIGRKFDDPDVQRDIKFLP 137
>At5g42020.2 68418.m05116 luminal binding protein 2 (BiP-2) (BP2)
similar to SWISS-PROT: Q39043; GI:1303695; luminal
binding protein (BiP) [Arabidopsis thaliana]
Length = 613
Score = 116 bits (280), Expect = 2e-26
Identities = 55/67 (82%), Positives = 59/67 (88%)
Frame = +1
Query: 289 IGTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTT 468
+G+VIGIDLGTTYSCVGVYKNG VEIIANDQGNRITPS+V FT D ERLIG+AAKNQ
Sbjct: 34 LGSVIGIDLGTTYSCVGVYKNGHVEIIANDQGNRITPSWVGFT-DSERLIGEAAKNQAAV 92
Query: 469 NPENTVF 489
NPE TVF
Sbjct: 93 NPERTVF 99
>At5g42020.1 68418.m05115 luminal binding protein 2 (BiP-2) (BP2)
similar to SWISS-PROT: Q39043; GI:1303695; luminal
binding protein (BiP) [Arabidopsis thaliana]
Length = 668
Score = 116 bits (280), Expect = 2e-26
Identities = 55/67 (82%), Positives = 59/67 (88%)
Frame = +1
Query: 289 IGTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTT 468
+G+VIGIDLGTTYSCVGVYKNG VEIIANDQGNRITPS+V FT D ERLIG+AAKNQ
Sbjct: 34 LGSVIGIDLGTTYSCVGVYKNGHVEIIANDQGNRITPSWVGFT-DSERLIGEAAKNQAAV 92
Query: 469 NPENTVF 489
NPE TVF
Sbjct: 93 NPERTVF 99
>At5g28540.1 68418.m03480 luminal binding protein 1 (BiP-1) (BP1)
SWISS-PROT:Q9LKR3 PMID:8888624
Length = 669
Score = 116 bits (280), Expect = 2e-26
Identities = 55/67 (82%), Positives = 59/67 (88%)
Frame = +1
Query: 289 IGTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTT 468
+G+VIGIDLGTTYSCVGVYKNG VEIIANDQGNRITPS+V FT D ERLIG+AAKNQ
Sbjct: 34 LGSVIGIDLGTTYSCVGVYKNGHVEIIANDQGNRITPSWVGFT-DSERLIGEAAKNQAAV 92
Query: 469 NPENTVF 489
NPE TVF
Sbjct: 93 NPERTVF 99
>At1g56410.1 68414.m06487 heat shock cognate 70 kDa protein,
putative / HSC70, putative / HSP70, putative strong
similarity to heat shock cognate 70 kd protein 1
SP:P22953 [Arabidopsis thaliana (Mouse-ear cress)]
Length = 617
Score = 111 bits (266), Expect = 8e-25
Identities = 58/90 (64%), Positives = 65/90 (72%)
Frame = +1
Query: 292 GTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTN 471
G IGIDLGTTYSCVGV+++ RVEIIANDQGNR TPSYVAFT D ERLIGDAAKNQ+ N
Sbjct: 7 GPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFT-DSERLIGDAAKNQVAMN 65
Query: 472 PENTVF*CPSV*LVXNXXXQPVXHDVKFLP 561
P NTVF + + V D+KF P
Sbjct: 66 PVNTVFDAKRL-IGRRFSDASVQSDMKFWP 94
>At3g12580.1 68416.m01567 heat shock protein 70, putative / HSP70,
putative strong similarity to heat shock protein
GI:425194 [Spinacia oleracea]
Length = 650
Score = 110 bits (265), Expect = 1e-24
Identities = 53/66 (80%), Positives = 57/66 (86%)
Frame = +1
Query: 292 GTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTN 471
G IGIDLGTTYSCVGV+++ RVEIIANDQGNR TPSYVAFT D ERLIGDAAKNQ+ N
Sbjct: 7 GPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFT-DSERLIGDAAKNQVAMN 65
Query: 472 PENTVF 489
P NTVF
Sbjct: 66 PTNTVF 71
>At5g02500.1 68418.m00183 heat shock cognate 70 kDa protein 1
(HSC70-1) (HSP70-1) identical to SP|P22953 Heat shock
cognate 70 kDa protein 1 (Hsc70.1) {Arabidopsis
thaliana}
Length = 651
Score = 110 bits (264), Expect = 1e-24
Identities = 53/66 (80%), Positives = 57/66 (86%)
Frame = +1
Query: 292 GTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTN 471
G IGIDLGTTYSCVGV+++ RVEIIANDQGNR TPSYVAFT D ERLIGDAAKNQ+ N
Sbjct: 7 GPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFT-DSERLIGDAAKNQVAMN 65
Query: 472 PENTVF 489
P NTVF
Sbjct: 66 PVNTVF 71
>At5g02490.1 68418.m00182 heat shock cognate 70 kDa protein 2
(HSC70-2) (HSP70-2) identical to SP|P22954 Heat shock
cognate 70 kDa protein 2 (Hsc70.2) {Arabidopsis
thaliana}
Length = 653
Score = 110 bits (264), Expect = 1e-24
Identities = 53/66 (80%), Positives = 57/66 (86%)
Frame = +1
Query: 292 GTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTN 471
G IGIDLGTTYSCVGV+++ RVEIIANDQGNR TPSYVAFT D ERLIGDAAKNQ+ N
Sbjct: 7 GPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFT-DSERLIGDAAKNQVAMN 65
Query: 472 PENTVF 489
P NTVF
Sbjct: 66 PVNTVF 71
>At3g09440.1 68416.m01121 heat shock cognate 70 kDa protein 3
(HSC70-3) (HSP70-3) identical to SP|O65719 Heat shock
cognate 70 kDa protein 3 (Hsc70.3) {Arabidopsis
thaliana}
Length = 649
Score = 109 bits (263), Expect = 2e-24
Identities = 53/66 (80%), Positives = 57/66 (86%)
Frame = +1
Query: 292 GTVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTN 471
G IGIDLGTTYSCVGV+++ RVEIIANDQGNR TPSYVAFT D ERLIGDAAKNQ+ N
Sbjct: 7 GPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFT-DSERLIGDAAKNQVAMN 65
Query: 472 PENTVF 489
P NTVF
Sbjct: 66 PINTVF 71
>At1g16030.1 68414.m01924 heat shock protein 70, putative / HSP70,
putative similar to heat shock protein hsp70 GI:1771478
from [Pisum sativum]
Length = 646
Score = 108 bits (259), Expect = 5e-24
Identities = 52/63 (82%), Positives = 55/63 (87%)
Frame = +1
Query: 301 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPEN 480
IGIDLGTTYSCVGV+ N RVEII NDQGNR TPSYVAFT D ERLIGDAAKNQ+ NP+N
Sbjct: 9 IGIDLGTTYSCVGVWMNDRVEIIPNDQGNRTTPSYVAFT-DTERLIGDAAKNQVALNPQN 67
Query: 481 TVF 489
TVF
Sbjct: 68 TVF 70
>At4g24280.1 68417.m03486 heat shock protein 70, putative / HSP70,
putative strong similarity to heat shock protein 70
[Arabidopsis thaliana] GI:6746592; similar to heat shock
70 protein - Spinacia oleracea,PID:g2654208
Length = 718
Score = 77.0 bits (181), Expect = 1e-14
Identities = 34/64 (53%), Positives = 43/64 (67%)
Frame = +1
Query: 298 VIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPE 477
V+GIDLGTT S V + G+ I+ N +G R TPS VA+T+ G+RL+G AK Q NPE
Sbjct: 80 VVGIDLGTTNSAVAAMEGGKPTIVTNAEGQRTTPSVVAYTKSGDRLVGQIAKRQAVVNPE 139
Query: 478 NTVF 489
NT F
Sbjct: 140 NTFF 143
>At4g37910.1 68417.m05361 heat shock protein 70, mitochondrial,
putative / HSP70, mitochondrial, putative strong
similarity to SP|Q01899 Heat shock 70 kDa protein,
mitochondrial precursor {Phaseolus vulgaris}
Length = 682
Score = 76.6 bits (180), Expect = 2e-14
Identities = 36/64 (56%), Positives = 42/64 (65%)
Frame = +1
Query: 298 VIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPE 477
VIGIDLGTT SCV V + +I N +G+R TPS VA Q GE L+G AK Q TNP
Sbjct: 54 VIGIDLGTTNSCVSVMEGKTARVIENAEGSRTTPSVVAMNQKGELLVGTPAKRQAVTNPT 113
Query: 478 NTVF 489
NT+F
Sbjct: 114 NTIF 117
>At5g49910.1 68418.m06180 heat shock protein 70 / HSP70 (HSC70-7)
identical to heat shock protein 70 [Arabidopsis
thaliana] GI:6746592
Length = 718
Score = 73.7 bits (173), Expect = 1e-13
Identities = 33/64 (51%), Positives = 42/64 (65%)
Frame = +1
Query: 298 VIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPE 477
V+GIDLGTT S V + G+ I+ N +G R TPS VA+T+ +RL+G AK Q NPE
Sbjct: 80 VVGIDLGTTNSAVAAMEGGKPTIVTNAEGQRTTPSVVAYTKSKDRLVGQIAKRQAVVNPE 139
Query: 478 NTVF 489
NT F
Sbjct: 140 NTFF 143
>At5g09590.1 68418.m01110 heat shock protein 70 / HSP70 (HSC70-5)
identical to heat shock protein 70 [Arabidopsis
thaliana] GI:6746590
Length = 682
Score = 73.3 bits (172), Expect = 2e-13
Identities = 36/63 (57%), Positives = 41/63 (65%)
Frame = +1
Query: 298 VIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNPE 477
VIGIDLGTT SCV V + ++I N +G R TPS VAF GE L+G AK Q TNP
Sbjct: 59 VIGIDLGTTNSCVAVMEGKNPKVIENAEGARTTPSVVAFNTKGELLVGTPAKRQAVTNPT 118
Query: 478 NTV 486
NTV
Sbjct: 119 NTV 121
>At1g79930.1 68414.m09340 heat shock protein, putative contains Pfam
profile: PF00012 Heat shock hsp70 proteins; similar to
heat-shock proteins GB:CAA94389, GB:AAD55461
[Arabidopsis thaliana]
Length = 831
Score = 47.6 bits (108), Expect = 1e-05
Identities = 22/64 (34%), Positives = 35/64 (54%)
Frame = +1
Query: 295 TVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNP 474
+V+G D G V V + ++++ ND+ NR TP+ V F D +R IG A NP
Sbjct: 2 SVVGFDFGNENCLVAVARQRGIDVVLNDESNRETPAIVCF-GDKQRFIGTAGAASTMMNP 60
Query: 475 ENTV 486
+N++
Sbjct: 61 KNSI 64
>At1g79920.2 68414.m09339 heat shock protein 70, putative / HSP70,
putative contains Pfam profile: PF00012 Heat shock hsp70
proteins; similar to heat-shock proteins GB:CAA94389,
GB:AAD55461 [Arabidopsis thaliana]
Length = 736
Score = 47.6 bits (108), Expect = 1e-05
Identities = 22/64 (34%), Positives = 35/64 (54%)
Frame = +1
Query: 295 TVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNP 474
+V+G D G V V + ++++ ND+ NR TP+ V F D +R IG A NP
Sbjct: 2 SVVGFDFGNENCLVAVARQRGIDVVLNDESNRETPAIVCF-GDKQRFIGTAGAASTMMNP 60
Query: 475 ENTV 486
+N++
Sbjct: 61 KNSI 64
>At1g79920.1 68414.m09338 heat shock protein 70, putative / HSP70,
putative contains Pfam profile: PF00012 Heat shock hsp70
proteins; similar to heat-shock proteins GB:CAA94389,
GB:AAD55461 [Arabidopsis thaliana]
Length = 736
Score = 47.6 bits (108), Expect = 1e-05
Identities = 22/64 (34%), Positives = 35/64 (54%)
Frame = +1
Query: 295 TVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNP 474
+V+G D G V V + ++++ ND+ NR TP+ V F D +R IG A NP
Sbjct: 2 SVVGFDFGNENCLVAVARQRGIDVVLNDESNRETPAIVCF-GDKQRFIGTAGAASTMMNP 60
Query: 475 ENTV 486
+N++
Sbjct: 61 KNSI 64
>At1g11660.1 68414.m01339 heat shock protein, putative strong
similarity to gb|Z70314 heat-shock protein from
Arabidopsis thaliana and is a member of the PF|00012
Hsp70 protein family
Length = 773
Score = 45.6 bits (103), Expect = 4e-05
Identities = 20/64 (31%), Positives = 38/64 (59%)
Frame = +1
Query: 295 TVIGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQDGERLIGDAAKNQLTTNP 474
+V+G D+G + V K ++++ ND+ NR P+ V+F + +R +G AA T +P
Sbjct: 2 SVVGFDVGNENCVIAVAKQRGIDVLLNDESNRENPAMVSFGEK-QRFMGAAAAASATMHP 60
Query: 475 ENTV 486
++T+
Sbjct: 61 KSTI 64
>At2g32120.2 68415.m03926 heat shock protein 70 family protein /
HSP70 family protein similar to SP|P22953 Heat shock
cognate 70 kDa protein 1 (Hsc70.1) {Arabidopsis
thaliana}; contains InterPro accession IPR001023: Heat
shock protein Hsp70
Length = 563
Score = 33.9 bits (74), Expect = 0.14
Identities = 12/41 (29%), Positives = 24/41 (58%)
Frame = +1
Query: 301 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQD 423
+GID+GT+ + V+ +V I+ N + ++ S+V F +
Sbjct: 31 LGIDIGTSQCSIAVWNGSQVHILRNTRNQKLIKSFVTFKDE 71
>At2g32120.1 68415.m03925 heat shock protein 70 family protein /
HSP70 family protein similar to SP|P22953 Heat shock
cognate 70 kDa protein 1 (Hsc70.1) {Arabidopsis
thaliana}; contains InterPro accession IPR001023: Heat
shock protein Hsp70
Length = 563
Score = 33.9 bits (74), Expect = 0.14
Identities = 12/41 (29%), Positives = 24/41 (58%)
Frame = +1
Query: 301 IGIDLGTTYSCVGVYKNGRVEIIANDQGNRITPSYVAFTQD 423
+GID+GT+ + V+ +V I+ N + ++ S+V F +
Sbjct: 31 LGIDIGTSQCSIAVWNGSQVHILRNTRNQKLIKSFVTFKDE 71
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,687,157
Number of Sequences: 28952
Number of extensions: 300029
Number of successful extensions: 608
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 561
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 592
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1980143200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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