Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_E08
(840 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 36 0.007
SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 35 0.012
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 31 0.27
SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 30 0.36
SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 30 0.47
SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr... 29 0.62
SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |S... 29 0.82
SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 29 1.1
SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb... 28 1.4
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 28 1.9
SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch... 28 1.9
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 27 2.5
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 4.4
SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 27 4.4
SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizo... 27 4.4
SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation... 26 5.8
SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p... 26 7.6
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 26 7.6
>SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 642
Score = 35.9 bits (79), Expect = 0.007
Identities = 32/99 (32%), Positives = 52/99 (52%), Gaps = 4/99 (4%)
Frame = +3
Query: 207 IEHHTKEFHKTLEQQFNSLTKSKDAQDF---SKAWKDGSESVLQQLNAFAKSLQGALGDA 377
I+ K LEQ+ +L ++++A++ ++ + D S S +L A AK A DA
Sbjct: 98 IQPDEKTLQDLLEQRQVALREAREAEEELQRARQYNDRSTSEALELEARAKK---AAQDA 154
Query: 378 NGKAKEALEQSRQNIERTAEELRK-AHPDVEKNATALRE 491
A E +++ +IER+A K A + E+ ATALRE
Sbjct: 155 E-LASERAREAQSSIERSASLREKQAREEAERAATALRE 192
>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 238
Score = 35.1 bits (77), Expect = 0.012
Identities = 28/107 (26%), Positives = 55/107 (51%), Gaps = 6/107 (5%)
Frame = +3
Query: 237 TLEQQFNSLTKSKDAQD---FSKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQ 407
T+E ++SL KSK + F + ++ + +L N + + L + +A KA+++LEQ
Sbjct: 8 TIELDYDSL-KSKISNFNSIFDRFLQEERKKLLNNKNEYLRQLS-EINEAQKKAEKSLEQ 65
Query: 408 SRQNIERTAEELRKAHPD---VEKNATALREKLQAAVQNTVQESQKL 539
+ + E L K H + E+ + +EKL A ++ + S++L
Sbjct: 66 TEARKQNFTELLEKEHEEQAITEQEIFSFQEKLDAMLKRKQKLSEEL 112
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 30.7 bits (66), Expect = 0.27
Identities = 30/131 (22%), Positives = 53/131 (40%)
Frame = +3
Query: 90 PHSVSRQYIMAAKFVVLFACIALAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTK 269
P S S + A + L + V R F + +EH+ K+LE+Q + L +
Sbjct: 171 PSSSSCNLVNANSLDIYLNINNLKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQ 229
Query: 270 SKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRK 449
SKDA A S+ + S + +L N K++ +L+ ++ R
Sbjct: 230 SKDASSNLTACNRSGSSLSSNFYSSRLSKKTSLASLN-KSRASLQHKIMSLSRNIIRRVF 288
Query: 450 AHPDVEKNATA 482
P+V + +A
Sbjct: 289 HKPEVHLDPSA 299
>SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 419
Score = 30.3 bits (65), Expect = 0.36
Identities = 17/81 (20%), Positives = 38/81 (46%)
Frame = +3
Query: 336 NAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQN 515
N + ++ AL + KA + LE+ ++ E + EE+ H + T+ + + +
Sbjct: 337 NLVSLAIYEALYEKFLKACKDLEEVSKSYEESREEIEALHETFTEEVTSFQSTKRLKEEK 396
Query: 516 TVQESQKLAKKVSSNVQETNE 578
+QE ++ K + Q+ +E
Sbjct: 397 IIQEKSRVDKMIDEYRQKLSE 417
>SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 168
Score = 29.9 bits (64), Expect = 0.47
Identities = 13/46 (28%), Positives = 25/46 (54%)
Frame = +3
Query: 303 KDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEE 440
KDG+++ +LN FAK L + + +A + + IE+++ E
Sbjct: 111 KDGTDAFANELNLFAKKLGFSKNSFDARALDTESEDETEIEKSSSE 156
>SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 633
Score = 29.5 bits (63), Expect = 0.62
Identities = 25/79 (31%), Positives = 37/79 (46%), Gaps = 8/79 (10%)
Frame = +3
Query: 384 KAKEALE---QSRQNIERTAEELRKAHPDVEKNATALREKLQAAV-----QNTVQESQKL 539
K KE +E Q ++ +ER E LRK D K+ + +AA+ + + E QKL
Sbjct: 128 KEKEEMEGSLQGKEKLEREVENLRK-ELDKYKDLVETEAEKRAAITKEECEKSWLEQQKL 186
Query: 540 AKKVSSNVQETNEKLAPKI 596
K + T +KL KI
Sbjct: 187 YKDMEQENASTIQKLTSKI 205
>SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 272
Score = 29.1 bits (62), Expect = 0.82
Identities = 17/69 (24%), Positives = 38/69 (55%)
Frame = +3
Query: 384 KAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNV 563
K+++ LE S Q +E E + P+V++ +EK ++ V+ +E +K+S N+
Sbjct: 132 KSEKPLETS-QKVEIETVETKPGEPEVKQETNLQKEKKESKVKLESKE-----EKISRNL 185
Query: 564 QETNEKLAP 590
+ ++ ++P
Sbjct: 186 RSSSRSISP 194
>SPBC646.09c |int6|yin6|translation initiation factor
eIF3e|Schizosaccharomyces pombe|chr 2|||Manual
Length = 501
Score = 28.7 bits (61), Expect = 1.1
Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
Frame = +3
Query: 282 QDFSKAWKDGSESVLQQLNAFAKSLQGALGD-ANGKAKEALEQSR-QNIERTAE 437
+D + A+ + SVLQ+L+ + +QG LG N AL Q + QN++ E
Sbjct: 79 EDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALRQDKGQNLQHLQE 132
>SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 175
Score = 28.3 bits (60), Expect = 1.4
Identities = 24/88 (27%), Positives = 45/88 (51%)
Frame = +3
Query: 207 IEHHTKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANGK 386
IE K F K ++ NS K+ +A F +G++ ++ + A ++L L +
Sbjct: 73 IESSMKSF-KPVKIDLNSQLKAINA--FEAKASEGAKKNVELVKAELQNLSATLKN---- 125
Query: 387 AKEALEQSRQNIERTAEELRKAHPDVEK 470
+EQ+R E T E++++A P++EK
Sbjct: 126 ----IEQARPTEEITIEDMKQAVPEIEK 149
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 27.9 bits (59), Expect = 1.9
Identities = 18/70 (25%), Positives = 32/70 (45%), Gaps = 3/70 (4%)
Frame = +3
Query: 177 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT---KSKDAQDFSKAWKDGSESVLQQLNAFA 347
++ DFFK + ++ H TL ++ NSL+ +K + G + +LN
Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLSSFLSTKSTSASENKFHGGLNWLSLKLNLLL 115
Query: 348 KSLQGALGDA 377
+ LQG + A
Sbjct: 116 R-LQGRMNSA 124
>SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 803
Score = 27.9 bits (59), Expect = 1.9
Identities = 24/74 (32%), Positives = 33/74 (44%), Gaps = 6/74 (8%)
Frame = +3
Query: 237 TLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGA------LGDANGKAKEA 398
T++ + SL K D + ++ ES L L L A L D+ KAK
Sbjct: 354 TIQIELESLRKETDTTSVER--REKLESKLTDLKEEQDKLSAAWEEERKLLDSIKKAKTE 411
Query: 399 LEQSRQNIERTAEE 440
LEQ+R +ERT E
Sbjct: 412 LEQARIELERTQRE 425
>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 631
Score = 27.5 bits (58), Expect = 2.5
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -1
Query: 414 ASTVPKPPWPCRSRLRALPG 355
AST+ K PWP + L +PG
Sbjct: 38 ASTLEKEPWPASTALLVMPG 57
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.6 bits (56), Expect = 4.4
Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
Frame = +3
Query: 183 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 302
D +F D++ H K FH E+ + + +K D K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706
>SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 670
Score = 26.6 bits (56), Expect = 4.4
Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 5/57 (8%)
Frame = +3
Query: 351 SLQGALGDANGKAKEALEQSRQNIE-----RTAEELRKAHPDVEKNATALREKLQAA 506
S++ L + N + KE +E + RT +E EKN LRE+L+ A
Sbjct: 520 SMKDDLTEMNQRLKEQIESYENEVNSEITSRTLKEFETLKTQYEKNLCNLREQLKTA 576
>SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase
Swr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1288
Score = 26.6 bits (56), Expect = 4.4
Identities = 22/74 (29%), Positives = 32/74 (43%)
Frame = +3
Query: 312 SESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALRE 491
S + + A K L GA KAKE ++ R +RTA E+RK +E+ R
Sbjct: 143 SRRISGMILAHFKRLSGA---DEKKAKEEDKRIRLLAKRTAWEIRKKWKVIEREVRRRRA 199
Query: 492 KLQAAVQNTVQESQ 533
+ A Q + Q
Sbjct: 200 ERAAEAQRVAGKEQ 213
>SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation
factor eIF2 beta subunit|Schizosaccharomyces pombe|chr
1|||Manual
Length = 321
Score = 26.2 bits (55), Expect = 5.8
Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
Frame = +3
Query: 219 TKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQ-GALGDA 377
+K+ + E+Q +T DFS K + L+AF K L+ + GDA
Sbjct: 89 SKKSSASAEEQTEDITTESGELDFSSMKKKKKKKKSADLSAFEKELEASSTGDA 142
>SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 25.8 bits (54), Expect = 7.6
Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +3
Query: 147 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 323
C + +G +RR P +++ I ++ KTL + FN+ + +++ + KA ++G V
Sbjct: 13 CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72
Query: 324 LQQL 335
Q+
Sbjct: 73 NNQI 76
>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1957
Score = 25.8 bits (54), Expect = 7.6
Identities = 18/77 (23%), Positives = 35/77 (45%), Gaps = 3/77 (3%)
Frame = +3
Query: 231 HKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANG---KAKEAL 401
H+T+ +Q + +A + ES L N ++ L ++N K +E +
Sbjct: 625 HQTITKQLKDTSSKLQQLQLERANFEQKESTLSDENNDLRTKLLKLEESNKSLIKKQEDV 684
Query: 402 EQSRQNIERTAEELRKA 452
+ +NI+ E+LRK+
Sbjct: 685 DSLEKNIQTLKEDLRKS 701
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,319,522
Number of Sequences: 5004
Number of extensions: 35556
Number of successful extensions: 177
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 176
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 414453330
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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