Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_E07
(827 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g34670.1 68417.m04922 40S ribosomal protein S3A (RPS3aB) 201 5e-52
At3g04840.1 68416.m00525 40S ribosomal protein S3A (RPS3aA) simi... 200 9e-52
At3g52640.1 68416.m05799 nicastrin-related contains weak similar... 30 1.6
At2g16250.1 68415.m01861 leucine-rich repeat transmembrane prote... 29 5.0
At3g09000.1 68416.m01053 proline-rich family protein 28 8.7
>At4g34670.1 68417.m04922 40S ribosomal protein S3A (RPS3aB)
Length = 262
Score = 201 bits (490), Expect = 5e-52
Identities = 104/188 (55%), Positives = 136/188 (72%), Gaps = 3/188 (1%)
Frame = +1
Query: 232 TKIASEGLKGRVFEVSLADLQADTDAERSFRKFRLIAEYVQGRNVLCNFHGMDLTTDKLR 411
TKIASEGLK RVFEVSLADLQ D D ++RK RL AE VQGRNVL F GMD TTDKLR
Sbjct: 54 TKIASEGLKHRVFEVSLADLQNDED--NAYRKIRLRAEDVQGRNVLTQFWGMDFTTDKLR 111
Query: 412 WMVKKWQTLIEANIDVKTTDGYVLRVFCIGFTNKDSLSQRKTCYAQHTQVRAIRKKMCEI 591
+VKKWQTLIEA++DVKTTDGY LR+FCI FT + + ++TCYAQ +Q+R IR+KM EI
Sbjct: 112 SLVKKWQTLIEAHVDVKTTDGYTLRMFCIAFTKRRANQVKRTCYAQSSQIRQIRRKMSEI 171
Query: 592 ITRDVTNSELREVVNKLIPDSIAKDIEKACHGI*PSARCLHPKXESVEXAP---LSEISK 762
+ ++ ++ +L+E+V K IP++I ++IEKA GI P K + ++ AP L ++ +
Sbjct: 172 MVKEASSCDLKELVAKFIPEAIGREIEKATQGIYPLQNVFIRKVKILK-APKFDLGKLME 230
Query: 763 XDGTFTGE 786
G +T E
Sbjct: 231 VHGDYTAE 238
Score = 33.9 bits (74), Expect = 0.13
Identities = 12/14 (85%), Positives = 14/14 (100%)
Frame = +2
Query: 131 VDPFTRKDWYDVKA 172
VDPF++KDWYDVKA
Sbjct: 21 VDPFSKKDWYDVKA 34
Score = 29.9 bits (64), Expect = 2.2
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = +3
Query: 168 RPPSMFSKRQVGTTLVNRTQGNE 236
+ P F+ R VG TLV+RTQG +
Sbjct: 33 KAPGSFTNRNVGKTLVSRTQGTK 55
>At3g04840.1 68416.m00525 40S ribosomal protein S3A (RPS3aA) similar
to 40S ribosomal protein S3A (S phase specific protein
GBIS289) GB:P49396 [Brassica rapa]
Length = 262
Score = 200 bits (488), Expect = 9e-52
Identities = 100/177 (56%), Positives = 131/177 (74%)
Frame = +1
Query: 232 TKIASEGLKGRVFEVSLADLQADTDAERSFRKFRLIAEYVQGRNVLCNFHGMDLTTDKLR 411
TKIASEGLK RVFEVSLADLQ D D ++RK RL AE VQGRNVLC F GMD TTDKLR
Sbjct: 54 TKIASEGLKHRVFEVSLADLQGDED--NAYRKIRLRAEDVQGRNVLCQFWGMDFTTDKLR 111
Query: 412 WMVKKWQTLIEANIDVKTTDGYVLRVFCIGFTNKDSLSQRKTCYAQHTQVRAIRKKMCEI 591
+VKKWQTLIEA++DVKTTD Y LR+FCI FT + + ++TCYAQ +Q+R IR+KM +I
Sbjct: 112 SLVKKWQTLIEAHVDVKTTDSYTLRLFCIAFTKRRANQVKRTCYAQSSQIRQIRRKMRDI 171
Query: 592 ITRDVTNSELREVVNKLIPDSIAKDIEKACHGI*PSARCLHPKXESVEXAPLSEISK 762
+ R+ ++ +L+++V K IP++I ++IEKA GI P K + ++ AP ++ K
Sbjct: 172 MVREASSCDLKDLVAKFIPEAIGREIEKATQGIYPLQNVFIRKVKILK-APKFDLGK 227
Score = 34.7 bits (76), Expect = 0.076
Identities = 13/25 (52%), Positives = 19/25 (76%), Gaps = 1/25 (4%)
Frame = +2
Query: 131 VDPFTRKDWYDVKA-SVYVQQEASR 202
VDPF++KDWYDVKA S++ + +
Sbjct: 21 VDPFSKKDWYDVKAPSIFTHRNVGK 45
Score = 31.9 bits (69), Expect = 0.53
Identities = 13/23 (56%), Positives = 18/23 (78%)
Frame = +3
Query: 168 RPPSMFSKRQVGTTLVNRTQGNE 236
+ PS+F+ R VG TLV+RTQG +
Sbjct: 33 KAPSIFTHRNVGKTLVSRTQGTK 55
>At3g52640.1 68416.m05799 nicastrin-related contains weak similarity
to Nicastrin precursor (Swiss-Prot:Q92542) [Homo
sapiens]
Length = 676
Score = 30.3 bits (65), Expect = 1.6
Identities = 19/58 (32%), Positives = 32/58 (55%), Gaps = 3/58 (5%)
Frame = -3
Query: 594 NDFTH-FLSDCSDLSVLGVARLA--LAQGVLIGETNAEDT*NVSIGCLHINVGFDESL 430
N F H L D S+++ V A +A+ + I ++ +DT N ++G +H+N F E L
Sbjct: 430 NKFYHSHLDDLSNINSSSVVAAASVVARTLYILASDNKDTSNSALGSIHVNASFVEEL 487
>At2g16250.1 68415.m01861 leucine-rich repeat transmembrane protein
kinase, putative
Length = 915
Score = 28.7 bits (61), Expect = 5.0
Identities = 17/55 (30%), Positives = 26/55 (47%)
Frame = -3
Query: 732 NTFTFRMQTSRRGLDAMAGLLDVLGNGVRNQLVHHLPEFRVSDVACNDFTHFLSD 568
N FT + S D +A ++D+ N +L L FR+ D++ N F L D
Sbjct: 331 NNFTGLLPYSSYDSDQIAEMVDISSNTFYGELTPILRRFRIMDLSGNYFEGKLPD 385
>At3g09000.1 68416.m01053 proline-rich family protein
Length = 541
Score = 27.9 bits (59), Expect = 8.7
Identities = 28/109 (25%), Positives = 55/109 (50%)
Frame = +2
Query: 368 SATSTAWTSQPISSGGWLKNGRLSSKPTLM*RQPMDTFYVSSALVSPIRTP*ANARRATP 547
S++S A +P SSG + R +S+P R+ +++ P+ T +N+R +TP
Sbjct: 137 SSSSVAGLRRPSSSG----SSRSTSRPATPTRRSTTP---TTSTSRPVTTRASNSRSSTP 189
Query: 548 STLRSEQSERKCVKSLHATSLTLNSGRW*TS*FLTPLPRTSRRPAMASN 694
++ + + R + + T +SG ++ TP R++ RP+ AS+
Sbjct: 190 TSRATLTAARATTSTAAPRTTTTSSG---SARSATP-TRSNPRPSSASS 234
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,002,979
Number of Sequences: 28952
Number of extensions: 381518
Number of successful extensions: 1072
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 995
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1068
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1902108000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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