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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP03_F_E05
         (872 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Sc...    29   1.1  
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces...    27   4.6  
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos...    27   4.6  
SPAC222.15 |meu13|SPAC821.01|Tat binding protein 1|Schizosacchar...    26   6.1  
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|...    26   6.1  
SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyce...    26   8.1  
SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p...    26   8.1  
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb...    26   8.1  

>SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 762

 Score = 28.7 bits (61), Expect = 1.1
 Identities = 13/31 (41%), Positives = 18/31 (58%)
 Frame = -3

Query: 432 LGRALDVLPRLFQSLLGLAVRVSERSPGDSW 340
           +G  +  L RLF SL GL    ++ SPG+ W
Sbjct: 410 VGTVIQGLSRLFSSLYGLRFVPADISPGEVW 440


>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1485

 Score = 26.6 bits (56), Expect = 4.6
 Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
 Frame = +3

Query: 177 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 296
           D   +F D++ H K FH   E+  +   +  +K   D  K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706


>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
           Plc1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 899

 Score = 26.6 bits (56), Expect = 4.6
 Identities = 10/30 (33%), Positives = 18/30 (60%)
 Frame = +3

Query: 171 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT 260
           ++   DFFK +    ++ H TL ++ NSL+
Sbjct: 56  KKSEQDFFKMLSSRDRDAHSTLRKRSNSLS 85


>SPAC222.15 |meu13|SPAC821.01|Tat binding protein
           1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 216

 Score = 26.2 bits (55), Expect = 6.1
 Identities = 16/56 (28%), Positives = 28/56 (50%)
 Frame = +2

Query: 452 LNVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQETNEKLAPKIKAAYDDFAK 619
           LN   +   +REK+Q+  +   + S KL    +  V++ +++   K    Y DFAK
Sbjct: 120 LNNSLSPAEIREKIQSIDKEIEETSSKLESLRNGTVKQISKEAMQKTDKNY-DFAK 174


>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 412

 Score = 26.2 bits (55), Expect = 6.1
 Identities = 19/64 (29%), Positives = 29/64 (45%)
 Frame = +3

Query: 84  PHSVSRQYIMAAKFVVLFACIALAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTK 263
           P S S   + A    +      L +   V R    F + +EH+     K+LE+Q + L +
Sbjct: 171 PSSSSCNLVNANSLDIYLNINNLKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQ 229

Query: 264 SKDA 275
           SKDA
Sbjct: 230 SKDA 233


>SPBC609.05 |pob3||FACT complex component Pob3|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 512

 Score = 25.8 bits (54), Expect = 8.1
 Identities = 12/24 (50%), Positives = 17/24 (70%), Gaps = 2/24 (8%)
 Frame = +2

Query: 542 KVSSNVQET--NEKLAPKIKAAYD 607
           +V  N++ET   EK A K+KA+YD
Sbjct: 299 EVDLNIEETVLKEKYADKVKASYD 322


>SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 394

 Score = 25.8 bits (54), Expect = 8.1
 Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
 Frame = +3

Query: 141 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 317
           C  + +G  +RR  P +++ I      ++ KTL + FN+  + +++  + KA ++G   V
Sbjct: 13  CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72

Query: 318 LQQL 329
             Q+
Sbjct: 73  NNQI 76


>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 631

 Score = 25.8 bits (54), Expect = 8.1
 Identities = 12/41 (29%), Positives = 22/41 (53%)
 Frame = -2

Query: 409 ASTVPKPPWPCRSRLRALPWRLLAKALSCCSTDSEPSFQAL 287
           AST+ K PWP  + L  +P     + +  CS+ +E  ++ +
Sbjct: 38  ASTLEKEPWPASTALLVMPG---GRDMGYCSSFNETIYRKI 75


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,240,680
Number of Sequences: 5004
Number of extensions: 38398
Number of successful extensions: 155
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 436477420
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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