BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_C18
(973 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces po... 33 0.080
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 30 0.57
SPBP35G2.07 |ilv1||acetolactate synthase catalytic subunit|Schiz... 28 1.7
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 28 2.3
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 27 3.0
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 27 3.0
SPAC23H4.10c |thi4||thiamine-phosphate dipyrophosphorylase/hydro... 27 4.0
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 26 7.0
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 9.2
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 26 9.2
>SPBP23A10.11c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 507
Score = 32.7 bits (71), Expect = 0.080
Identities = 13/31 (41%), Positives = 21/31 (67%)
Frame = +1
Query: 43 YNTKYNQYLKMSTTTCNCNSRDRVVYGGNSA 135
Y++ YN+ M T++C+C+S + YGGN A
Sbjct: 47 YSSTYNEITNMDTSSCSCSSTPK-SYGGNLA 76
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 29.9 bits (64), Expect = 0.57
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = +3
Query: 405 PPXPPPPXXXPPPXPP 452
PP PPPP PP PP
Sbjct: 10 PPPPPPPGFEPPSQPP 25
Score = 27.1 bits (57), Expect = 4.0
Identities = 11/19 (57%), Positives = 11/19 (57%), Gaps = 3/19 (15%)
Frame = +3
Query: 405 PPXPP---PPXXXPPPXPP 452
PP PP PP PPP PP
Sbjct: 12 PPPPPGFEPPSQPPPPPPP 30
Score = 26.6 bits (56), Expect = 5.3
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +3
Query: 405 PPXPPPPXXXPPPXPP 452
PP PPPP PP P
Sbjct: 9 PPPPPPPPGFEPPSQP 24
>SPBP35G2.07 |ilv1||acetolactate synthase catalytic
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 669
Score = 28.3 bits (60), Expect = 1.7
Identities = 13/42 (30%), Positives = 21/42 (50%)
Frame = -1
Query: 289 SPSCPTALRSPEAFTIVPSSQGIVELAIVDEEQDVVFVLGGL 164
+P CPT LR +P + ++ L VDE D+ + G+
Sbjct: 309 NPECPTLLRKFSERLQIPVTTSLLGLGAVDERSDLSLHMLGM 350
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.9 bits (59), Expect = 2.3
Identities = 11/23 (47%), Positives = 11/23 (47%)
Frame = +3
Query: 405 PPXPPPPXXXPPPXPPXTXXQVP 473
PP P P PP PP T VP
Sbjct: 1198 PPVPKPSVGVPPVPPPSTAPPVP 1220
Score = 26.2 bits (55), Expect = 7.0
Identities = 11/29 (37%), Positives = 11/29 (37%)
Frame = +3
Query: 405 PPXPPPPXXXPPPXPPXTXXQVPGXXGXP 491
PP P P P P P VP G P
Sbjct: 1131 PPVPVPSGAPPVPKPSVAAPPVPAPSGAP 1159
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 27.5 bits (58), Expect = 3.0
Identities = 12/24 (50%), Positives = 13/24 (54%)
Frame = -1
Query: 475 PGTWXXVXGGXGGGXXXGGGGXGG 404
PG + GG GGG GGG GG
Sbjct: 230 PGGFEGGPGGFGGGPGGFGGGLGG 253
Score = 27.5 bits (58), Expect = 3.0
Identities = 12/24 (50%), Positives = 13/24 (54%)
Frame = -1
Query: 475 PGTWXXVXGGXGGGXXXGGGGXGG 404
PG + GG GGG GGG GG
Sbjct: 237 PGGFGGGPGGFGGGLGGFGGGPGG 260
Score = 27.5 bits (58), Expect = 3.0
Identities = 12/24 (50%), Positives = 13/24 (54%)
Frame = -1
Query: 475 PGTWXXVXGGXGGGXXXGGGGXGG 404
PG + GG GGG GGG GG
Sbjct: 244 PGGFGGGLGGFGGGPGGFGGGPGG 267
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 27.5 bits (58), Expect = 3.0
Identities = 12/34 (35%), Positives = 13/34 (38%)
Frame = +3
Query: 390 LTKXXPPXPPPPXXXPPPXPPXTXXQVPGXXGXP 491
L K PP P PP PP + G G P
Sbjct: 1890 LPKAGPPSAAPTSALPPAGPPAGATAISGNPGMP 1923
>SPAC23H4.10c |thi4||thiamine-phosphate
dipyrophosphorylase/hydroxyethylthiazole kinase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 518
Score = 27.1 bits (57), Expect = 4.0
Identities = 15/36 (41%), Positives = 19/36 (52%)
Frame = +1
Query: 250 TPRETARPLDTMVKSPVFLTSTRGSLHLSKRPKTLL 357
TPRETA+ L ++ +P R SL PK LL
Sbjct: 207 TPRETAKELRNLIATPPCFAQARSSL---TTPKDLL 239
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 26.2 bits (55), Expect = 7.0
Identities = 11/20 (55%), Positives = 11/20 (55%), Gaps = 4/20 (20%)
Frame = +3
Query: 405 PPXPPPP----XXXPPPXPP 452
PP PPPP PPP PP
Sbjct: 761 PPPPPPPPGVAGAGPPPPPP 780
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 25.8 bits (54), Expect = 9.2
Identities = 11/24 (45%), Positives = 12/24 (50%), Gaps = 1/24 (4%)
Frame = +3
Query: 405 PPXPP-PPXXXPPPXPPXTXXQVP 473
PP P PP PPP P + VP
Sbjct: 1721 PPMPAGPPSAPPPPLPASSAPSVP 1744
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 25.8 bits (54), Expect = 9.2
Identities = 11/26 (42%), Positives = 13/26 (50%)
Frame = +2
Query: 365 MNNVINEHSYQTXXPXTSPPPXXPPP 442
+N V N+ S P PPP PPP
Sbjct: 928 VNIVANDTSVAGVMPAFPPPPPPPPP 953
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,596,899
Number of Sequences: 5004
Number of extensions: 40342
Number of successful extensions: 234
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 101
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 182
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 499296500
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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