Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_C04
(863 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 137 2e-33
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 137 2e-33
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 105 6e-24
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 58 2e-09
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 54 3e-08
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 46 5e-06
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 42 9e-05
SPAC1B2.05 |mcm5|nda4, SPAC3F10.01|MCM complex subunit Mcm5|Schi... 30 0.37
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 28 1.5
SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 27 3.4
SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonucl... 26 7.9
SPAC1093.01 ||SPAC12B10.18|PPR repeat protein|Schizosaccharomyce... 26 7.9
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 137 bits (331), Expect = 2e-33
Identities = 70/103 (67%), Positives = 83/103 (80%), Gaps = 1/103 (0%)
Frame = +1
Query: 199 VDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKSTAISMFFELEEKDLV 378
VDHGKSTLTDSLV KAGII+ A+AG+ RF DTR DEQ+R +TIKSTAIS+F E+ + D+
Sbjct: 28 VDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQERGVTIKSTAISLFAEMTDDDM- 86
Query: 379 FITNPDQREKSE-KGFLINLIDSPGHVDFSSEVTAALRVTDGA 504
D +E ++ FL+NLIDSPGHVDFSSEVTAALRVTDGA
Sbjct: 87 ----KDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALRVTDGA 125
Score = 95.9 bits (228), Expect = 7e-21
Identities = 45/85 (52%), Positives = 59/85 (69%)
Frame = +2
Query: 545 TETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYNDDGGP 724
TETVLRQA+ ERI+P++ +NK+DR YQ F R+VE+VNV+I+TY D
Sbjct: 139 TETVLRQALGERIRPVVVVNKVDRALLELQISQEELYQNFARVVESVNVVISTYYDK--V 196
Query: 725 MGEVRVDPSKGSVGFGSGLHGWAFT 799
+G+ +V P KG+V F SGLHGWAFT
Sbjct: 197 LGDCQVFPDKGTVAFASGLHGWAFT 221
Score = 33.5 bits (73), Expect = 0.040
Identities = 13/21 (61%), Positives = 17/21 (80%)
Frame = +3
Query: 135 DEIRGMMDKKRNIRNMSVIAH 197
+E+R +M K N+RNMSVIAH
Sbjct: 7 EEVRNLMGKPSNVRNMSVIAH 27
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 137 bits (331), Expect = 2e-33
Identities = 70/103 (67%), Positives = 83/103 (80%), Gaps = 1/103 (0%)
Frame = +1
Query: 199 VDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKSTAISMFFELEEKDLV 378
VDHGKSTLTDSLV KAGII+ A+AG+ RF DTR DEQ+R +TIKSTAIS+F E+ + D+
Sbjct: 28 VDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQERGVTIKSTAISLFAEMTDDDM- 86
Query: 379 FITNPDQREKSE-KGFLINLIDSPGHVDFSSEVTAALRVTDGA 504
D +E ++ FL+NLIDSPGHVDFSSEVTAALRVTDGA
Sbjct: 87 ----KDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALRVTDGA 125
Score = 95.9 bits (228), Expect = 7e-21
Identities = 45/85 (52%), Positives = 59/85 (69%)
Frame = +2
Query: 545 TETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYNDDGGP 724
TETVLRQA+ ERI+P++ +NK+DR YQ F R+VE+VNV+I+TY D
Sbjct: 139 TETVLRQALGERIRPVVVVNKVDRALLELQISQEELYQNFARVVESVNVVISTYYDK--V 196
Query: 725 MGEVRVDPSKGSVGFGSGLHGWAFT 799
+G+ +V P KG+V F SGLHGWAFT
Sbjct: 197 LGDCQVFPDKGTVAFASGLHGWAFT 221
Score = 33.5 bits (73), Expect = 0.040
Identities = 13/21 (61%), Positives = 17/21 (80%)
Frame = +3
Query: 135 DEIRGMMDKKRNIRNMSVIAH 197
+E+R +M K N+RNMSVIAH
Sbjct: 7 EEVRNLMGKPSNVRNMSVIAH 27
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 105 bits (253), Expect = 6e-24
Identities = 57/102 (55%), Positives = 75/102 (73%)
Frame = +1
Query: 199 VDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKSTAISMFFELEEKDLV 378
VDHGK+TL DSL++ GII+ AG RF D R+DE R IT+KS+AIS+FF++
Sbjct: 28 VDHGKTTLADSLLASNGIISSKLAGTVRFLDFREDEITRGITMKSSAISLFFKV------ 81
Query: 379 FITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGA 504
I+ D++ + EK +LINLIDSPGHVDFSSEV++A R+ DGA
Sbjct: 82 -ISQNDEK-RVEKDYLINLIDSPGHVDFSSEVSSASRLCDGA 121
Score = 52.0 bits (119), Expect = 1e-07
Identities = 35/94 (37%), Positives = 45/94 (47%), Gaps = 10/94 (10%)
Frame = +2
Query: 545 TETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATY------ 706
T TVLRQA +RIK IL +NKMDR + R+VE VN +I T+
Sbjct: 135 TITVLRQAWIDRIKVILVINKMDRLITELKLSPIEAHYHLLRLVEQVNAVIGTFYTGELM 194
Query: 707 ----NDDGGPMGEVRVDPSKGSVGFGSGLHGWAF 796
ND+ + P +G+V F S GWAF
Sbjct: 195 QLADNDEVISDEGIYFAPEQGNVVFASAYDGWAF 228
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 58.0 bits (134), Expect = 2e-09
Identities = 39/110 (35%), Positives = 58/110 (52%), Gaps = 5/110 (4%)
Frame = +1
Query: 190 SPTVDHGKSTLTDSLVSKAGIIAGARA--GETRF---TDTRKDEQDRCITIKSTAISMFF 354
S +D GK+T T+ ++ G I G+ D + E+++ ITI+S A +
Sbjct: 65 SAHIDSGKTTFTERVLYYTGRIKDIHEVRGKDNVGAKMDFMELEREKGITIQSAATHCTW 124
Query: 355 ELEEKDLVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGA 504
E + N Q+ EK + IN+ID+PGH+DF+ EV ALRV DGA
Sbjct: 125 ERTVDQIE--ANEKQKTDFEKSYNINIIDTPGHIDFTIEVERALRVLDGA 172
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 54.0 bits (124), Expect = 3e-08
Identities = 34/101 (33%), Positives = 52/101 (51%)
Frame = +1
Query: 199 VDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKSTAISMFFELEEKDLV 378
+DHGKSTL+D ++ G+I +F D + E+ R IT+K+ SM +
Sbjct: 67 IDHGKSTLSDCILKLTGVI-NEHNFRNQFLDKLEVERRRGITVKAQTCSMIYYYH----- 120
Query: 379 FITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDG 501
+ +L+NLID+PGHVDF +EV +L +G
Sbjct: 121 -----------GQSYLLNLIDTPGHVDFRAEVMHSLAACEG 150
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 46.4 bits (105), Expect = 5e-06
Identities = 34/100 (34%), Positives = 49/100 (49%), Gaps = 1/100 (1%)
Frame = +1
Query: 205 HGKSTLTDSLVSKAGI-IAGARAGETRFTDTRKDEQDRCITIKSTAISMFFELEEKDLVF 381
HGKS L D LV + R+TDT E++R ++IKST +++
Sbjct: 151 HGKSALLDLLVYYTHPDTKPPKRRSLRYTDTHYLERERVMSIKSTPLTLAVS-------- 202
Query: 382 ITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDG 501
D + K+ F ID+PGHVDF EV A + ++DG
Sbjct: 203 ----DMKGKT---FAFQCIDTPGHVDFVDEVAAPMAISDG 235
Score = 35.5 bits (78), Expect = 0.010
Identities = 23/85 (27%), Positives = 38/85 (44%)
Frame = +2
Query: 545 TETVLRQAIAERIKPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYNDDGGP 724
T +++ AI + +L +NK+DR Y + +++ VN I + D
Sbjct: 250 TTRIIKHAILHDMPIVLVLNKVDRLILELRLPPNDAYHKLRHVIDEVNDNICQISKD--- 306
Query: 725 MGEVRVDPSKGSVGFGSGLHGWAFT 799
+ RV P G+V F S G+ FT
Sbjct: 307 -LKYRVSPELGNVCFASCDLGYCFT 330
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 42.3 bits (95), Expect = 9e-05
Identities = 37/104 (35%), Positives = 51/104 (49%), Gaps = 2/104 (1%)
Frame = +1
Query: 199 VDHGKSTLTDSLVSKAGIIAGARAGETRFT--DTRKDEQDRCITIKSTAISMFFELEEKD 372
+D GK+TLT+ ++ G + +T T D E+ R ITI S AIS +
Sbjct: 37 IDAGKTTLTEKMLYYGGFTSHFGNVDTGDTVMDYLPAERQRGITINSAAISFTWR----- 91
Query: 373 LVFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTAALRVTDGA 504
+QR INLID+PGH DF+ EV ++ V DGA
Sbjct: 92 -------NQR--------INLIDTPGHADFTFEVERSVAVLDGA 120
Score = 26.6 bits (56), Expect = 4.5
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +2
Query: 545 TETVLRQAIAERIKPILFMNKMDR 616
T+ V +QA I ++F+NKMDR
Sbjct: 134 TKVVWKQATKRGIPKVIFVNKMDR 157
>SPAC1B2.05 |mcm5|nda4, SPAC3F10.01|MCM complex subunit
Mcm5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 30.3 bits (65), Expect = 0.37
Identities = 23/95 (24%), Positives = 45/95 (47%)
Frame = +1
Query: 196 TVDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKSTAISMFFELEEKDL 375
T+ K+ +T L S+ ++A A R+ D + ++ I +ST +S F D+
Sbjct: 457 TISIAKAGITTILNSRTSVLAAANPIFGRYDDMKTPGEN--IDFQSTILSRF------DM 508
Query: 376 VFITNPDQREKSEKGFLINLIDSPGHVDFSSEVTA 480
+FI + E ++ ++I+ ++ SSE A
Sbjct: 509 IFIVKDEHDETKDRNIARHVINLHTNLQESSETLA 543
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 28.3 bits (60), Expect = 1.5
Identities = 16/48 (33%), Positives = 24/48 (50%)
Frame = +1
Query: 199 VDHGKSTLTDSLVSKAGIIAGARAGETRFTDTRKDEQDRCITIKSTAI 342
VDHGK+TLT ++ + A + D +E+ R ITI S +
Sbjct: 62 VDHGKTTLTAAITKCLSDLGQASFMDYSQIDKAPEEKARGITISSAHV 109
>SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr
2|||Manual
Length = 207
Score = 27.1 bits (57), Expect = 3.4
Identities = 18/60 (30%), Positives = 30/60 (50%), Gaps = 1/60 (1%)
Frame = -1
Query: 467 EEKSTCPGESIKLIKKPFSLFSRWSGFVMNTKSFSS-SSKNIEMAVDLMVMQRSCSSLRV 291
EE + P + K KK S F+ T +S S++NI+ A+DL+ + S S ++
Sbjct: 66 EEMESLPSKGGKGSKKAAKKNSSLDAFLNETPQTASYSARNIDDALDLLSLNNSSSKDKI 125
>SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting
endonuclease Cce1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 258
Score = 25.8 bits (54), Expect = 7.9
Identities = 14/37 (37%), Positives = 22/37 (59%), Gaps = 1/37 (2%)
Frame = -1
Query: 419 PFSLFSRWSGFVMNTK-SFSSSSKNIEMAVDLMVMQR 312
P S +S W+ V+NTK SFS ++M +L+ Q+
Sbjct: 168 PKSTYSYWAS-VLNTKASFSKKKSRVQMVKELIDGQK 203
>SPAC1093.01 ||SPAC12B10.18|PPR repeat protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1261
Score = 25.8 bits (54), Expect = 7.9
Identities = 13/47 (27%), Positives = 23/47 (48%)
Frame = -2
Query: 175 RIFRFLSIIPRISSTREIHHFRWFMIFVLLNQLPYASNGVRFEKANL 35
R+F + R ST+ + WFM +L + +S +F+ +NL
Sbjct: 828 RVFEHSKHLYRKISTKSLEKANWFMALILDAMILSSSFARQFKSSNL 874
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,422,875
Number of Sequences: 5004
Number of extensions: 69729
Number of successful extensions: 238
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 212
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 232
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 430470850
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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