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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP03_F_B22
         (859 letters)

Database: bee 
           438 sequences; 146,343 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ667186-1|ABG75738.1|  447|Apis mellifera glutamate-gated chlor...    27   0.22 
DQ667185-1|ABG75737.1|  447|Apis mellifera glutamate-gated chlor...    25   1.2  
DQ325076-1|ABD14090.1|  191|Apis mellifera complementary sex det...    24   2.1  
AJ968562-1|CAI91546.1|  998|Apis mellifera protein ( Apis mellif...    23   3.6  
DQ026034-1|AAY87893.1|  569|Apis mellifera nicotinic acetylcholi...    22   8.3  
DQ026033-1|AAY87892.1|  569|Apis mellifera nicotinic acetylcholi...    22   8.3  

>DQ667186-1|ABG75738.1|  447|Apis mellifera glutamate-gated chloride
           channel protein.
          Length = 447

 Score = 27.1 bits (57), Expect = 0.22
 Identities = 22/87 (25%), Positives = 40/87 (45%)
 Frame = +3

Query: 75  PNKMKLLVVFAMCMLAASAGVVELSADTSNQDLEEKLYNSILTGDYDSAVRQSLEYESQG 254
           P  +KLLV+    +  +           + ++ E+++ ++IL G YD+ +R S E  + G
Sbjct: 3   PGVLKLLVLLTFLLHPSRC----TQGKVNYREKEKEVLDNIL-GGYDARIRPSGENATDG 57

Query: 255 KGSIIQNVVNNLIIDKRRNTMEYCYKL 335
              +  N+    I      TMEY  +L
Sbjct: 58  PAVVRVNIFVRSISKIDDVTMEYSVQL 84


>DQ667185-1|ABG75737.1|  447|Apis mellifera glutamate-gated chloride
           channel protein.
          Length = 447

 Score = 24.6 bits (51), Expect = 1.2
 Identities = 21/87 (24%), Positives = 39/87 (44%)
 Frame = +3

Query: 75  PNKMKLLVVFAMCMLAASAGVVELSADTSNQDLEEKLYNSILTGDYDSAVRQSLEYESQG 254
           P  +KLLV+    +  +           + ++ E+++ ++IL G YD+ +R S E  + G
Sbjct: 3   PGVLKLLVLLTFLLHPSRC----TQGKVNYREKEKEVLDNIL-GGYDARIRPSGENATDG 57

Query: 255 KGSIIQNVVNNLIIDKRRNTMEYCYKL 335
              +  N+    I       MEY  +L
Sbjct: 58  PAIVRVNLFVRSIATISDIKMEYSVQL 84


>DQ325076-1|ABD14090.1|  191|Apis mellifera complementary sex
           determiner protein.
          Length = 191

 Score = 23.8 bits (49), Expect = 2.1
 Identities = 9/37 (24%), Positives = 20/37 (54%)
 Frame = +1

Query: 499 VXTSILNSSVGSSLPWWENNRVYFKIHNTKYNQYLKM 609
           + +S+ N ++ ++  +  NN   +  +N  YN Y K+
Sbjct: 81  IISSLSNKTIHNNNNYNNNNYNNYNYNNNNYNNYKKL 117


>AJ968562-1|CAI91546.1|  998|Apis mellifera protein ( Apis mellifera
           ORF for hypotheticalprotein. ).
          Length = 998

 Score = 23.0 bits (47), Expect = 3.6
 Identities = 11/30 (36%), Positives = 18/30 (60%)
 Frame = -1

Query: 151 ADSSTTPALAASMHIANTTRSFILLGAFQI 62
           A+   +  +A  +HI+N T SF L G F++
Sbjct: 396 ANKMESSGMAGRVHISNATLSF-LNGEFEV 424


>DQ026034-1|AAY87893.1|  569|Apis mellifera nicotinic acetylcholine
           receptor alpha4subunit protein.
          Length = 569

 Score = 21.8 bits (44), Expect = 8.3
 Identities = 16/54 (29%), Positives = 24/54 (44%)
 Frame = -3

Query: 209 VAGEDAVVQFLLEVLVRSVRG*FNDARAGGEHAHRKYNEKFHFVRSLSDRLQVC 48
           + GE   V FL  ++V        DA+   +     YN+    V + SD L+VC
Sbjct: 4   IIGETLRVWFLSALVVHGAVAGNPDAKRLYDDLLSNYNKLVRPVVNTSDVLRVC 57


>DQ026033-1|AAY87892.1|  569|Apis mellifera nicotinic acetylcholine
           receptor alpha4subunit protein.
          Length = 569

 Score = 21.8 bits (44), Expect = 8.3
 Identities = 16/54 (29%), Positives = 24/54 (44%)
 Frame = -3

Query: 209 VAGEDAVVQFLLEVLVRSVRG*FNDARAGGEHAHRKYNEKFHFVRSLSDRLQVC 48
           + GE   V FL  ++V        DA+   +     YN+    V + SD L+VC
Sbjct: 4   IIGETLRVWFLSALVVHGAVAGNPDAKRLYDDLLSNYNKLVRPVVNTSDVLRVC 57


  Database: bee
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 146,343
  Number of sequences in database:  438
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 222,633
Number of Sequences: 438
Number of extensions: 4537
Number of successful extensions: 15
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 15
length of database: 146,343
effective HSP length: 57
effective length of database: 121,377
effective search space used: 27673956
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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