BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_B18
(882 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6F6.05 |||oligosaccharyltransferase epsilon subunit |Schizos... 76 8e-15
SPCC1840.03 |sal3|pse1|karyopherin Sal3|Schizosaccharomyces pomb... 29 0.66
SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein |Schizo... 29 1.2
SPCC31H12.07 |sec231|sec23a, SPCC5E4.01|GTPase activating protei... 27 4.7
SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|ch... 27 4.7
SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic... 26 6.2
SPAC13D6.01 |pof14||F-box protein Pof14|Schizosaccharomyces pomb... 26 6.2
>SPAC6F6.05 |||oligosaccharyltransferase epsilon subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 122
Score = 75.8 bits (178), Expect = 8e-15
Identities = 44/110 (40%), Positives = 62/110 (56%), Gaps = 7/110 (6%)
Frame = +3
Query: 105 SSLTAVIPKLYQEYTTKTPKKLKIIDAYLFYIFLTAVIQFGYCCLVGTFPFNSFLSGFIS 284
S L + + Y T LK IDA+L ++ + +QFGY LVGT+PFNSFLSGFIS
Sbjct: 5 SGLFLPLSSVITSYNENTNLSLKTIDAFLGFLVVVGGLQFGYALLVGTYPFNSFLSGFIS 64
Query: 285 TVSCFVLGVCLRLQVNPE------NKNEFQGLSA-ERGFADFIFAHLVLH 413
V FV+ V R+ + + +K + +S +R F +F F+ LVLH
Sbjct: 65 CVGQFVITVGFRMALTQQELQSSSSKKKSPVVSPYKRAFLEFCFSSLVLH 114
>SPCC1840.03 |sal3|pse1|karyopherin Sal3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1095
Score = 29.5 bits (63), Expect = 0.66
Identities = 14/39 (35%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
Frame = -2
Query: 401 MCKYEICK-ATFSTQPLELILVFWINL*PKTHSKNKAAY 288
+CK IC+ + Q L+ ++ +WIN P TH ++ A Y
Sbjct: 980 ICK--ICRFCSQRVQDLDKVVTYWINTLPVTHDEDDAPY 1016
>SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 391
Score = 28.7 bits (61), Expect = 1.2
Identities = 22/72 (30%), Positives = 33/72 (45%), Gaps = 10/72 (13%)
Frame = -3
Query: 340 FSGLTCSRRHTPRTKQLTVDIKP-------LKKELNGKVPTRQ---Q*PNCMTAVRKI*K 191
F G+T RRH K DI P L KE+N Q + P+ ++R +
Sbjct: 36 FEGVTLQRRHVKGMKSYGSDITPRRPKQLGLPKEVNTSECIDQGSWRKPSAFESLRSYSR 95
Query: 190 RYASIILSFLGV 155
+++ I SF+GV
Sbjct: 96 KFSKRIFSFIGV 107
>SPCC31H12.07 |sec231|sec23a, SPCC5E4.01|GTPase activating protein
Sec23a|Schizosaccharomyces pombe|chr 3|||Manual
Length = 759
Score = 26.6 bits (56), Expect = 4.7
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = -3
Query: 136 YNFGITAVSEDILL*FYLFCNSK*YTSSQLSQILRIPYS 20
Y G T S+ Y+F SK YTS Q+ ++L +P S
Sbjct: 170 YELGYTECSKS-----YVFRGSKDYTSKQIQEMLGLPTS 203
>SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1822
Score = 26.6 bits (56), Expect = 4.7
Identities = 13/34 (38%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = +1
Query: 91 ITIKCLHLLL*FQNCIKNTQRK-LLRSLK*LMHT 189
+ IKC+ L+ + C+KN + K L+ LK L+ T
Sbjct: 143 VLIKCIEQLIQIKVCMKNEKMKNLMEQLKPLLQT 176
>SPBC19G7.05c |bgs1|cps1, drc1|1,3-beta-glucan synthase catalytic
subunit Bgs1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1729
Score = 26.2 bits (55), Expect = 6.2
Identities = 11/40 (27%), Positives = 18/40 (45%)
Frame = +3
Query: 126 PKLYQEYTTKTPKKLKIIDAYLFYIFLTAVIQFGYCCLVG 245
PK+ T L +D YL+YI ++ + Y +G
Sbjct: 557 PKILLSLLYLTDLSLFFLDTYLWYILISTIYSLAYAFCLG 596
>SPAC13D6.01 |pof14||F-box protein Pof14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 431
Score = 26.2 bits (55), Expect = 6.2
Identities = 13/33 (39%), Positives = 20/33 (60%), Gaps = 2/33 (6%)
Frame = +3
Query: 90 NHNKMSSLTAVIPK--LYQEYTTKTPKKLKIID 182
N + +LT PK LY Y++++PK KI+D
Sbjct: 139 NLDSSLNLTVASPKSNLYYRYSSESPKYAKILD 171
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,240,786
Number of Sequences: 5004
Number of extensions: 61902
Number of successful extensions: 146
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 146
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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