BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP03_F_B03 (851 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 34 0.029 SPAC343.04c |gnr1||heterotrimeric G protein beta subunit Gnr1|Sc... 29 0.63 SPCC576.13 |swc5||chromatin remodeling complex subunit Swc5|Schi... 29 0.84 SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr ... 28 1.9 SPAC12G12.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 27 3.4 SPAC17A5.10 |||conserved fungal protein|Schizosaccharomyces pomb... 27 3.4 SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc... 27 3.4 SPBC18A7.02c |||seven transmembrane receptor-like protein|Schizo... 26 5.9 SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosac... 26 7.8 SPAC1002.13c |psu1||beta-glucosidase Psu1 |Schizosaccharomyces p... 26 7.8 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 33.9 bits (74), Expect = 0.029 Identities = 23/71 (32%), Positives = 32/71 (45%) Frame = +3 Query: 486 VTPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*SSITRKVLXMTVSSTVIAPLTPS 665 VTP P S S PP +T T S P +++ S T + T +ST P+TP+ Sbjct: 355 VTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLLSTSTSCTTSTSIPPTGNST--TPVTPT 412 Query: 666 TPLVP*APPCT 698 P + P T Sbjct: 413 VPPTSSSTPLT 423 Score = 32.7 bits (71), Expect = 0.068 Identities = 21/62 (33%), Positives = 29/62 (46%) Frame = +3 Query: 486 VTPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*SSITRKVLXMTVSSTVIAPLTPS 665 VTP P S S PP +T T S P +++ S T + T +ST P+TP+ Sbjct: 301 VTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLPSTSTSCTTSTSIPPTGNST--TPVTPT 358 Query: 666 TP 671 P Sbjct: 359 VP 360 Score = 32.3 bits (70), Expect = 0.090 Identities = 21/63 (33%), Positives = 30/63 (47%), Gaps = 1/63 (1%) Frame = +3 Query: 486 VTPKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLXMTVSSTVIAPLTP 662 +TP P S S+P P T+ + S P T ST S T + SS++ P+TP Sbjct: 191 ITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTST-SCTTSTSIPTGGSSSLSTPITP 249 Query: 663 STP 671 + P Sbjct: 250 TVP 252 Score = 29.9 bits (64), Expect = 0.48 Identities = 22/63 (34%), Positives = 30/63 (47%), Gaps = 1/63 (1%) Frame = +3 Query: 486 VTPKTKPARKSPGSLP-PCWKTTEFTSRSCPPRTNST*SSITRKVLXMTVSSTVIAPLTP 662 +TP P S S+P P T+ + S P T ST S T + T +ST P+TP Sbjct: 247 ITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTST-SCTTSTSIPPTGNST--TPVTP 303 Query: 663 STP 671 + P Sbjct: 304 TVP 306 Score = 26.2 bits (55), Expect = 5.9 Identities = 22/67 (32%), Positives = 29/67 (43%), Gaps = 3/67 (4%) Frame = +3 Query: 477 LHSVTPKTKPARKSPGSLPPCW-KTTEFTSRSCPPRTNST*SSI--TRKVLXMTVSSTVI 647 L++ TP T P S S W TT T S T+ST + T + SST I Sbjct: 598 LYTSTPITSPNSTSSSSTQVSWNSTTPITGTSTSKVTSSTSIPLTSTNRTSTTFTSSTSI 657 Query: 648 APLTPST 668 + + ST Sbjct: 658 STSSSST 664 >SPAC343.04c |gnr1||heterotrimeric G protein beta subunit Gnr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 507 Score = 29.5 bits (63), Expect = 0.63 Identities = 15/34 (44%), Positives = 20/34 (58%) Frame = -2 Query: 568 DLEVNSVVFQHGGKLPGDFLAGFVFGVTECNFVV 467 DLE N +V Q+ G G+FL G FG + FV+ Sbjct: 411 DLEENRIVRQYMGHKLGNFLIGSCFGGKDDTFVL 444 >SPCC576.13 |swc5||chromatin remodeling complex subunit Swc5|Schizosaccharomyces pombe|chr 3|||Manual Length = 215 Score = 29.1 bits (62), Expect = 0.84 Identities = 15/48 (31%), Positives = 26/48 (54%) Frame = +2 Query: 212 ETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKDGKE 355 ET K S K +K + + +++++ K NT++ A Q W+K KE Sbjct: 128 ETPKKKHSLIRKRRKSPLDSSSAQKVLKKNKLNTLEQAQQNWSKYIKE 175 >SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1237 Score = 27.9 bits (59), Expect = 1.9 Identities = 12/40 (30%), Positives = 23/40 (57%), Gaps = 1/40 (2%) Frame = +2 Query: 179 QLYMSVVIGEY-ETAIAKCSEYLKEKKGEVIKEAVKRLIE 295 Q+++++V+ Y A+ EYL+EK I + K ++E Sbjct: 556 QIFINIVLPNYIRAALVVAKEYLREKANADINDLTKDMLE 595 >SPAC12G12.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 977 Score = 27.1 bits (57), Expect = 3.4 Identities = 12/30 (40%), Positives = 18/30 (60%), Gaps = 1/30 (3%) Frame = +2 Query: 650 TADTFNTTGTLSPSMYESXVMFXVYN-REY 736 T FN +GTL+P ++ S V + N RE+ Sbjct: 915 TLSVFNNSGTLTPELFSSVVRIVLKNVREF 944 >SPAC17A5.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 224 Score = 27.1 bits (57), Expect = 3.4 Identities = 12/25 (48%), Positives = 15/25 (60%) Frame = -1 Query: 812 PXXPPRVSRLLVGGPYLSFKCHNTG 738 P PR+S G Y+ +KCHNTG Sbjct: 127 PSGRPRISYPYPPG-YMCYKCHNTG 150 >SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces pombe|chr 1|||Manual Length = 283 Score = 27.1 bits (57), Expect = 3.4 Identities = 12/34 (35%), Positives = 19/34 (55%) Frame = +2 Query: 647 STADTFNTTGTLSPSMYESXVMFXVYNREYNQCY 748 S + T +TT TL+ M + F + +YN+CY Sbjct: 2 SESQTESTTVTLTNGMVIPRIGFGAFMLKYNECY 35 >SPBC18A7.02c |||seven transmembrane receptor-like protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 457 Score = 26.2 bits (55), Expect = 5.9 Identities = 19/78 (24%), Positives = 37/78 (47%) Frame = -3 Query: 273 SLITSPFFSFRYSEHLAIAVSYSPMTTLIYSCSASTSSVLGASVALEASAHTARTKANKV 94 SL+ S FFS YSE + + + IYS S S S+ + A+ + + ++V Sbjct: 6 SLLWSIFFSIVYSEKTLLNFKHYELCNGIYSKSESGGSL---NPAIYVNWTEPWGQEDEV 62 Query: 93 SLILAQWLSLKAVTTLKS 40 +++ W ++ + +S Sbjct: 63 EVLIFNWKEIRKLGAFRS 80 >SPCC5E4.06 |smc6|rad18|Smc5-6 complex SMC subunit Smc6|Schizosaccharomyces pombe|chr 3|||Manual Length = 1140 Score = 25.8 bits (54), Expect = 7.8 Identities = 12/31 (38%), Positives = 17/31 (54%) Frame = +2 Query: 416 LINKRDHHALKLIDQQNHNKIAFGDSKDKTS 508 L+ K+D A K+ DQ H K+ +DK S Sbjct: 496 LLTKKDSIANKISDQSEHLKVLEDVQRDKVS 526 >SPAC1002.13c |psu1||beta-glucosidase Psu1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 417 Score = 25.8 bits (54), Expect = 7.8 Identities = 16/71 (22%), Positives = 31/71 (43%) Frame = -3 Query: 315 MVFLLPFSIRRFTASLITSPFFSFRYSEHLAIAVSYSPMTTLIYSCSASTSSVLGASVAL 136 M F ++ T + +PF R+ HL S +T+ +Y+ + T ++++ Sbjct: 1 MRFFETLALALLTTGALAAPF---RHPHHLLNKRDVSVVTSKVYAYTTVTLEAAASAIST 57 Query: 135 EASAHTARTKA 103 +A A T A Sbjct: 58 NGAAKEAATAA 68 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,379,283 Number of Sequences: 5004 Number of extensions: 70012 Number of successful extensions: 261 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 239 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 258 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 422462090 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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