Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_B03
(851 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g18670.1 68417.m02762 leucine-rich repeat family protein / ex... 34 0.14
At1g14070.1 68414.m01664 xyloglucan fucosyltransferase, putative... 33 0.24
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 31 0.97
At1g64150.1 68414.m07267 expressed protein contains Pfam profile... 30 1.7
At1g62250.2 68414.m07023 expressed protein 30 1.7
At1g62250.1 68414.m07022 expressed protein 30 1.7
At5g09400.1 68418.m01089 potassium transporter family protein si... 29 3.9
At1g77240.1 68414.m08996 AMP-binding protein, putative strong si... 29 3.9
At1g14110.1 68414.m01668 xyloglucan fucosyltransferase family pr... 29 3.9
At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase (AT... 29 5.2
At4g13290.1 68417.m02078 cytochrome P450 71A19, putative (CYP71A... 29 5.2
At1g47310.1 68414.m05238 expressed protein 28 6.9
At1g14080.1 68414.m01666 xyloglucan fucosyltransferase, putative... 28 6.9
At3g62000.1 68416.m06963 O-methyltransferase family 3 protein se... 28 9.1
At3g04160.1 68416.m00440 expressed protein ; expression supporte... 28 9.1
At2g25290.1 68415.m03025 octicosapeptide/Phox/Bem1p (PB1) domain... 28 9.1
At2g15350.1 68415.m01756 xyloglucan fucosyltransferase, putative... 28 9.1
>At4g18670.1 68417.m02762 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 839
Score = 33.9 bits (74), Expect = 0.14
Identities = 22/69 (31%), Positives = 32/69 (46%), Gaps = 1/69 (1%)
Frame = +3
Query: 489 TPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*S-SITRKVLXMTVSSTVIAPLTPS 665
+P + P +PG PP TT T PP + +T S + ++ S + P PS
Sbjct: 480 SPPSSPTTPTPGGSPPSSPTTP-TPGGSPPSSPTTPSPGGSPPSPSISPSPPITVPSPPS 538
Query: 666 TPLVP*APP 692
TP P +PP
Sbjct: 539 TPTSPGSPP 547
Score = 29.1 bits (62), Expect = 3.9
Identities = 22/74 (29%), Positives = 32/74 (43%), Gaps = 4/74 (5%)
Frame = +3
Query: 489 TPKTKPARKSPGSLPPCWKTTEFTSRSCPPRTNST*SSITRKVLXMTVSSTVIAP----L 656
+P T P +PG PP TT T PP + +T + T S +P +
Sbjct: 467 SPPTSPTTPTPGGSPPSSPTTP-TPGGSPPSSPTTPTPGGSPPSSPTTPSPGGSPPSPSI 525
Query: 657 TPSTPLVP*APPCT 698
+PS P+ +PP T
Sbjct: 526 SPSPPITVPSPPST 539
>At1g14070.1 68414.m01664 xyloglucan fucosyltransferase, putative
(FUT7) nearly identical to SP|Q9XI81 Probable
fucosyltransferase 7 (EC 2.4.1.-) (AtFUT7) {Arabidopsis
thaliana}; similar to xyloglucan fucosyltransferase
GI:5231145 from (Arabidopsis thaliana)
Length = 509
Score = 33.1 bits (72), Expect = 0.24
Identities = 29/112 (25%), Positives = 42/112 (37%)
Frame = +2
Query: 455 DQQNHNKIAFGDSKDKTSKKVSWKFTPVLENNRVYFKIMSTEDKQYLKLDNTKGSXDDRI 634
++ H+ GDS D K + W L NR+ + Y L D R
Sbjct: 95 EKLGHDHENVGDSSDGECKYIVWVAVYGL-GNRI---LTLASVFLYALLTERIILVDQRK 150
Query: 635 IYGDSTADTFNTTGTLSPSMYESXVMFXVYNREYNQCYDT*MKDMARQPTTV 790
D + F T L P + +NREY+ CY T +K+ TT+
Sbjct: 151 DISDLFCEPFPGTSWLLPLDFPLMGQIDSFNREYSHCYGTMLKNHTINSTTI 202
>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
/ VPS11 family protein similar to Vacuolar protein
sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
sapiens]; similar to Vacuolar biogenesis protein END1
(PEP5 protein) (Vacuolar protein sorting 11)
(Swiss-Prot:P12868) [Saccharomyces cerevisiae]
Length = 932
Score = 31.1 bits (67), Expect = 0.97
Identities = 21/85 (24%), Positives = 40/85 (47%), Gaps = 4/85 (4%)
Frame = +2
Query: 200 IGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKDG--KEIVKSYF 373
+G Y+ A+ S + G I++ K LIE+ + T+D +L T+ G + S
Sbjct: 498 LGNYDEALQYVSSLEPSQAGVTIEQYGKILIEHKPKETIDILMRLCTEQGIPNGVFLSML 557
Query: 374 --PIQFRVIFTEQTVKLINKRDHHA 442
P+ F +F + L++ + +A
Sbjct: 558 PSPVDFITVFVQHPHSLMHFLERYA 582
>At1g64150.1 68414.m07267 expressed protein contains Pfam profile
PF01169: Uncharacterized protein family UPF0016
Length = 370
Score = 30.3 bits (65), Expect = 1.7
Identities = 13/41 (31%), Positives = 22/41 (53%)
Frame = -2
Query: 670 GVEGVSGAITVDDTVIXRTFRVIELQVLFVLGGHDLEVNSV 548
G G G +T+ V+ RTF ++ + F GG DL ++ +
Sbjct: 194 GTFGALGIMTIISVVLGRTFHYVDEVLPFRFGGTDLPIDDI 234
>At1g62250.2 68414.m07023 expressed protein
Length = 223
Score = 30.3 bits (65), Expect = 1.7
Identities = 12/43 (27%), Positives = 23/43 (53%)
Frame = +3
Query: 96 LCLPSSSPCVRWLLTPHLHQELMTYWRSSCI*VSSLVNTRPLS 224
LC P + +RW TP + E+++ WR C +++ R ++
Sbjct: 181 LCTPQPT-VIRWSSTPSVSDEILSKWRGFCAVIANAYYIRGMA 222
>At1g62250.1 68414.m07022 expressed protein
Length = 267
Score = 30.3 bits (65), Expect = 1.7
Identities = 12/43 (27%), Positives = 23/43 (53%)
Frame = +3
Query: 96 LCLPSSSPCVRWLLTPHLHQELMTYWRSSCI*VSSLVNTRPLS 224
LC P + +RW TP + E+++ WR C +++ R ++
Sbjct: 181 LCTPQPT-VIRWSSTPSVSDEILSKWRGFCAVIANAYYIRGMA 222
>At5g09400.1 68418.m01089 potassium transporter family protein
similar to K+ transporter HAK5 [Arabidopsis thaliana]
GI:7108597; contains Pfam profile PF02705: K+ potassium
transporter; KUP/HAK/KT Transporter family member,
PMID:11500563; Note: possible sequencing error causes a
frameshift in the 4th exon|15810448|gb|AY056263
Length = 858
Score = 29.1 bits (62), Expect = 3.9
Identities = 19/73 (26%), Positives = 35/73 (47%)
Frame = -3
Query: 282 FTASLITSPFFSFRYSEHLAIAVSYSPMTTLIYSCSASTSSVLGASVALEASAHTARTKA 103
F +S+ S F+ + ++A ++ MTT +SC S+ LG L+ HT+R
Sbjct: 411 FFSSVPGSAFWPVLFIANIAALIASRTMTTATFSC-IKQSTALGCFPRLKI-IHTSRKFM 468
Query: 102 NKVSLILAQWLSL 64
++ + + W L
Sbjct: 469 GQIYIPVLNWFLL 481
>At1g77240.1 68414.m08996 AMP-binding protein, putative strong
similarity to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 545
Score = 29.1 bits (62), Expect = 3.9
Identities = 19/67 (28%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
Frame = +2
Query: 359 VKSYFPIQFRVIFTEQTVKLINKR-DHHALKLIDQQNHNKIAFGDSKDKTSKKVSWKFTP 535
V S + +QF V + + IN R D HAL ++ + + +K+ F D + + F P
Sbjct: 76 VPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVDHHSSSLVLEAVSFLP 135
Query: 536 VLENNRV 556
E R+
Sbjct: 136 KDERPRL 142
>At1g14110.1 68414.m01668 xyloglucan fucosyltransferase family
protein contains Pfam profile: PF03254 xyloglucan
fucosyltransferase
Length = 907
Score = 29.1 bits (62), Expect = 3.9
Identities = 16/58 (27%), Positives = 26/58 (44%)
Frame = +2
Query: 623 DDRIIYGDSTADTFNTTGTLSPSMYESXVMFXVYNREYNQCYDT*MKDMARQPTTVKP 796
D R D + F T L P + +NRE+++CY T +K+ T++ P
Sbjct: 629 DQRNDITDLFCEPFPETSWLLPLDFPLNDQLDSFNREHSRCYGTMLKNHGINSTSIIP 686
>At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase
(ATHIM) identical to SP|P34881 DNA
(cytosine-5)-methyltransferase AthI (EC 2.1.1.37)
{Arabidopsis thaliana}
Length = 1534
Score = 28.7 bits (61), Expect = 5.2
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = -1
Query: 344 PLSITGRRSPWCSSCRFRSDASR 276
PLS GR S +C+SC+ R D +
Sbjct: 869 PLSDIGRSSGFCTSCKIREDEEK 891
>At4g13290.1 68417.m02078 cytochrome P450 71A19, putative (CYP71A19)
Identical to Cytochrome P450 (SP:Q9T0K0) [Arabidopsis
thaliana]; similar to cytochrome P450LXXIA1, Persea
americana, M32885
Length = 490
Score = 28.7 bits (61), Expect = 5.2
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = -2
Query: 442 GVMVPFVYELDSLLGEDHSKLDGEVRFDDFL 350
G +P + +D + G+DH + + RFD+FL
Sbjct: 221 GEYIPSLSWIDKIRGQDHKMEEVDKRFDEFL 251
>At1g47310.1 68414.m05238 expressed protein
Length = 395
Score = 28.3 bits (60), Expect = 6.9
Identities = 19/67 (28%), Positives = 35/67 (52%)
Frame = +2
Query: 398 TEQTVKLINKRDHHALKLIDQQNHNKIAFGDSKDKTSKKVSWKFTPVLENNRVYFKIMST 577
T QTV + + A ++ Q + + G ++ SKKV+W+ P +E RV+F++ +
Sbjct: 286 TRQTVSSVTAK-LKASGMVRFQLEIERSIGKNESVISKKVAWRTKPKIE--RVWFEVTAK 342
Query: 578 EDKQYLK 598
+ LK
Sbjct: 343 IEGDKLK 349
>At1g14080.1 68414.m01666 xyloglucan fucosyltransferase, putative
(FUT6) nearly identical to SP|Q9XI80 Probable
fucosyltransferase 6 (EC 2.4.1.-) (AtFUT6) {Arabidopsis
thaliana}; similar to xyloglucan fucosyltransferase
GI:5231145 from (Arabidopsis thaliana)
Length = 519
Score = 28.3 bits (60), Expect = 6.9
Identities = 14/51 (27%), Positives = 22/51 (43%)
Frame = +2
Query: 644 DSTADTFNTTGTLSPSMYESXVMFXVYNREYNQCYDT*MKDMARQPTTVKP 796
D + F T L P + YN+ Y++CY T + + A T + P
Sbjct: 169 DLFCEPFPGTSWLLPLEFPLMKQIDGYNKGYSRCYGTMLNNQAINSTLIPP 219
>At3g62000.1 68416.m06963 O-methyltransferase family 3 protein
several O-methyltransferases - different species;
contains Pfam 01596 O-methyltransferase domain
Length = 278
Score = 27.9 bits (59), Expect = 9.1
Identities = 26/126 (20%), Positives = 56/126 (44%), Gaps = 2/126 (1%)
Frame = +2
Query: 167 VLAEQLYMSVVIGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQLWTKD 346
V E+ S+ + + +A S + K+G + E++K +I+NG+ + DFA+
Sbjct: 147 VACERDSNSLEVAKRYYELAGVSHKVNVKQG-LAAESLKSMIQNGEGASYDFAF---VDA 202
Query: 347 GKEIVKSYFPIQFRVIFTEQTVKLINKRDHHALK--LIDQQNHNKIAFGDSKDKTSKKVS 520
K + + YF + +++ + + N H + +++ I + K K+VS
Sbjct: 203 DKRMYQDYFELLLQLVRVGGVIVMDNVLWHGRVSDPMVNDAKTISIRNFNKKLMDDKRVS 262
Query: 521 WKFTPV 538
P+
Sbjct: 263 ISMVPI 268
>At3g04160.1 68416.m00440 expressed protein ; expression supported
by MPSS
Length = 712
Score = 27.9 bits (59), Expect = 9.1
Identities = 11/37 (29%), Positives = 18/37 (48%)
Frame = +1
Query: 274 SREASDRKRQEEHHGLRLPVMDKGWKGNRQILLPHPV 384
SR ++E G +P DK WKG + + +P+
Sbjct: 563 SRSPIGNDQKESDFGYSIPSTDKQWKGENRADIEYPI 599
>At2g25290.1 68415.m03025 octicosapeptide/Phox/Bem1p (PB1)
domain-containing protein / tetratricopeptide repeat
(TPR)-containing protein low similarity to SP|Q99614
Tetratricopeptide repeat protein 1 {Homo sapiens};
contains Pfam profiles PF00564: PB1 domain, PF00515: TPR
Domain
Length = 697
Score = 27.9 bits (59), Expect = 9.1
Identities = 17/49 (34%), Positives = 25/49 (51%)
Frame = +2
Query: 185 YMSVVIGEYETAIAKCSEYLKEKKGEVIKEAVKRLIENGKRNTMDFAYQ 331
YM + +GEY AI +C+ L E K +KR N +DFA++
Sbjct: 100 YMQMGLGEYPNAINECNLAL-EASPRFSKALLKRARCYEALNKLDFAFR 147
>At2g15350.1 68415.m01756 xyloglucan fucosyltransferase, putative
(FUT10) identical to SP|Q9SJP6 Putative
fucosyltransferase 10 (EC 2.4.1.-) (AtFUT10) (Fragment)
{Arabidopsis thaliana}; similar to xyloglucan
fucosyltransferase GI:5231145 from [Arabidopsis
thaliana]
Length = 440
Score = 27.9 bits (59), Expect = 9.1
Identities = 10/25 (40%), Positives = 17/25 (68%)
Frame = +2
Query: 722 YNREYNQCYDT*MKDMARQPTTVKP 796
YN+EY +CY T +++ A T++ P
Sbjct: 118 YNKEYPRCYGTMLENHAINSTSIPP 142
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,193,061
Number of Sequences: 28952
Number of extensions: 384921
Number of successful extensions: 1307
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 1263
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1304
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1980143200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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