Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_A12
(962 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 36 0.009
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 35 0.015
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 32 0.10
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 30 0.42
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 27 0.61
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 0.74
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 27 3.0
SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex p... 27 3.0
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 27 3.9
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 3.9
SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 23 5.2
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 26 6.9
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 35.9 bits (79), Expect = 0.009
Identities = 14/26 (53%), Positives = 14/26 (53%)
Frame = +2
Query: 455 PPPPPXXAGXLXXPXPPPPPXXXPSG 532
PPPPP AG P PPPPP G
Sbjct: 764 PPPPPGVAGAGPPPPPPPPPAVSAGG 789
Score = 31.5 bits (68), Expect = 0.18
Identities = 14/31 (45%), Positives = 15/31 (48%), Gaps = 2/31 (6%)
Frame = +2
Query: 455 PPPPPXXAG--XLXXPXPPPPPXXXPSGXXR 541
PPPPP G P PPPPP +G R
Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPPPAVSAGGSR 791
Score = 30.7 bits (66), Expect = 0.32
Identities = 16/35 (45%), Positives = 16/35 (45%), Gaps = 3/35 (8%)
Frame = +3
Query: 450 IXXPPPRXXPAXXXPPXPPPPP---XXXPPAPXVP 545
I PPP P PP PPPPP PP P P
Sbjct: 749 IPVPPPA--PIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 28.7 bits (61), Expect = 1.3
Identities = 18/58 (31%), Positives = 19/58 (32%)
Frame = +3
Query: 459 PPPRXXPAXXXPPXPPPPPXXXPPAPXVPXGXGXXXXXPXXGGXTXXXPPXXAGGPPP 632
PPP P P P P P PP + G P G PP PPP
Sbjct: 732 PPP--PPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP----PPP 783
Score = 28.7 bits (61), Expect = 1.3
Identities = 14/32 (43%), Positives = 15/32 (46%)
Frame = +2
Query: 437 PA*XNXPPPPPXXAGXLXXPXPPPPPXXXPSG 532
PA PPP P G P PPPPP +G
Sbjct: 746 PAPIPVPPPAPIMGG---PPPPPPPPGVAGAG 774
Score = 27.5 bits (58), Expect = 3.0
Identities = 13/26 (50%), Positives = 14/26 (53%)
Frame = +2
Query: 437 PA*XNXPPPPPXXAGXLXXPXPPPPP 514
PA P PPP A + P PPPPP
Sbjct: 744 PAPAPIPVPPP--APIMGGPPPPPPP 767
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 35.1 bits (77), Expect = 0.015
Identities = 14/29 (48%), Positives = 14/29 (48%)
Frame = +3
Query: 459 PPPRXXPAXXXPPXPPPPPXXXPPAPXVP 545
PPP P PP PPPP AP VP
Sbjct: 1716 PPPSAPPMPAGPPSAPPPPLPASSAPSVP 1744
Score = 31.9 bits (69), Expect = 0.14
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = +3
Query: 462 PPRXXPAXXXPPXPPPPPXXXPPAPXVP 545
P P P PPPPP PP P P
Sbjct: 1694 PQSAAPPQMSAPTPPPPPMSVPPPPSAP 1721
Score = 30.3 bits (65), Expect = 0.42
Identities = 12/29 (41%), Positives = 12/29 (41%)
Frame = +3
Query: 459 PPPRXXPAXXXPPXPPPPPXXXPPAPXVP 545
PP P PP PPP PP P P
Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGP 1727
Score = 28.3 bits (60), Expect = 1.7
Identities = 15/42 (35%), Positives = 15/42 (35%)
Frame = +3
Query: 459 PPPRXXPAXXXPPXPPPPPXXXPPAPXVPXGXGXXXXXPXXG 584
PPP P PP PP P P AP P P G
Sbjct: 1709 PPPMSVPP---PPSAPPMPAGPPSAPPPPLPASSAPSVPNPG 1747
Score = 27.1 bits (57), Expect = 3.9
Identities = 13/33 (39%), Positives = 14/33 (42%), Gaps = 2/33 (6%)
Frame = +3
Query: 459 PPPRXXPAXXXPPXPPPPPXXXPP--APXVPXG 551
P P P PPPP PP AP +P G
Sbjct: 1694 PQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAG 1726
Score = 26.2 bits (55), Expect = 6.9
Identities = 13/27 (48%), Positives = 13/27 (48%), Gaps = 2/27 (7%)
Frame = +2
Query: 455 PPP--PPXXAGXLXXPXPPPPPXXXPS 529
PPP PP AG P PP P PS
Sbjct: 1716 PPPSAPPMPAGPPSAPPPPLPASSAPS 1742
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 32.3 bits (70), Expect = 0.10
Identities = 21/66 (31%), Positives = 22/66 (33%), Gaps = 5/66 (7%)
Frame = +3
Query: 462 PPRXXPAXXXPPXPPP---PPXXXPP--APXVPXGXGXXXXXPXXGGXTXXXPPXXAGGP 626
PP P+ PP P P PP P AP VP P PP P
Sbjct: 1140 PPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPP 1199
Query: 627 PPKXXV 644
PK V
Sbjct: 1200 VPKPSV 1205
Score = 30.3 bits (65), Expect = 0.42
Identities = 18/61 (29%), Positives = 20/61 (32%)
Frame = +3
Query: 462 PPRXXPAXXXPPXPPPPPXXXPPAPXVPXGXGXXXXXPXXGGXTXXXPPXXAGGPPPKXX 641
PP + P PPP P P +P G P G P A P PK
Sbjct: 1050 PPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSG-APPVPAPSGIPPVPKPSVAAPPVPKPS 1108
Query: 642 V 644
V
Sbjct: 1109 V 1109
Score = 28.7 bits (61), Expect = 1.3
Identities = 17/58 (29%), Positives = 17/58 (29%)
Frame = +3
Query: 459 PPPRXXPAXXXPPXPPPPPXXXPPAPXVPXGXGXXXXXPXXGGXTXXXPPXXAGGPPP 632
P P P P PP P AP VP P G P A P P
Sbjct: 1077 PAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVP 1134
Score = 27.9 bits (59), Expect = 2.3
Identities = 21/66 (31%), Positives = 24/66 (36%), Gaps = 4/66 (6%)
Frame = +3
Query: 459 PPPRXXPAXXXPPXPPPPPXXXPPAP-XVPXGXGXXXXXPXXGGXTXXXPPXXA--GGPP 629
PPP P+ P P P PAP +P P + PP A G PP
Sbjct: 1063 PPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPP 1122
Query: 630 -PKXXV 644
PK V
Sbjct: 1123 VPKPSV 1128
Score = 27.9 bits (59), Expect = 2.3
Identities = 19/57 (33%), Positives = 20/57 (35%)
Frame = +3
Query: 462 PPRXXPAXXXPPXPPPPPXXXPPAPXVPXGXGXXXXXPXXGGXTXXXPPXXAGGPPP 632
PP PA PP PPP PP P G P T P + G PP
Sbjct: 1179 PPVPKPAAGVPPVPPPSEA--PPVPKPSVG-----VPPVPPPSTAPPVPTPSAGLPP 1228
Score = 27.5 bits (58), Expect = 3.0
Identities = 15/33 (45%), Positives = 16/33 (48%), Gaps = 5/33 (15%)
Frame = +3
Query: 462 PPRXXPAXXXPPXPPP---PPXXXPPA--PXVP 545
PP P+ PP PPP PP P A P VP
Sbjct: 1198 PPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVP 1230
Score = 26.6 bits (56), Expect = 5.2
Identities = 20/63 (31%), Positives = 24/63 (38%), Gaps = 7/63 (11%)
Frame = +3
Query: 462 PPRXXPAXXXPPXPPP---PPXXXPPA-PXVP-XGXGXXXXXPXXGGXTXXXP--PXXAG 620
PP P+ PP P P PP P + P VP G P + P P +G
Sbjct: 1022 PPVPLPSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSG 1081
Query: 621 GPP 629
PP
Sbjct: 1082 APP 1084
Score = 26.6 bits (56), Expect = 5.2
Identities = 13/34 (38%), Positives = 13/34 (38%), Gaps = 3/34 (8%)
Frame = +3
Query: 459 PPPRXXPAXXXPP---XPPPPPXXXPPAPXVPXG 551
PPP P P P PPP PP P G
Sbjct: 1192 PPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAG 1225
Score = 26.2 bits (55), Expect = 6.9
Identities = 16/58 (27%), Positives = 16/58 (27%)
Frame = +3
Query: 459 PPPRXXPAXXXPPXPPPPPXXXPPAPXVPXGXGXXXXXPXXGGXTXXXPPXXAGGPPP 632
P P P P PP AP VP P G P A P P
Sbjct: 1115 PAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVP 1172
Score = 26.2 bits (55), Expect = 6.9
Identities = 16/57 (28%), Positives = 16/57 (28%)
Frame = +3
Query: 459 PPPRXXPAXXXPPXPPPPPXXXPPAPXVPXGXGXXXXXPXXGGXTXXXPPXXAGGPP 629
P P P P PP AP VP P P AG PP
Sbjct: 1134 PVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPP 1190
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 30.3 bits (65), Expect = 0.42
Identities = 11/20 (55%), Positives = 11/20 (55%)
Frame = +2
Query: 455 PPPPPXXAGXLXXPXPPPPP 514
PPPPP G PPPPP
Sbjct: 9 PPPPPPPPGFEPPSQPPPPP 28
Score = 29.9 bits (64), Expect = 0.56
Identities = 11/20 (55%), Positives = 11/20 (55%)
Frame = +2
Query: 455 PPPPPXXAGXLXXPXPPPPP 514
PPPPP P PPPPP
Sbjct: 11 PPPPPPGFEPPSQPPPPPPP 30
Score = 29.5 bits (63), Expect = 0.74
Identities = 10/18 (55%), Positives = 11/18 (61%)
Frame = +3
Query: 492 PPXPPPPPXXXPPAPXVP 545
PP PPPPP PP+ P
Sbjct: 9 PPPPPPPPGFEPPSQPPP 26
Score = 29.1 bits (62), Expect = 0.98
Identities = 11/22 (50%), Positives = 11/22 (50%)
Frame = +2
Query: 449 NXPPPPPXXAGXLXXPXPPPPP 514
N PPPPP PPPPP
Sbjct: 8 NPPPPPPPPGFEPPSQPPPPPP 29
Score = 27.1 bits (57), Expect = 3.9
Identities = 12/26 (46%), Positives = 12/26 (46%), Gaps = 3/26 (11%)
Frame = +3
Query: 477 PAXXXPPXPPPP---PXXXPPAPXVP 545
P PP PPPP P PP P P
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPP 30
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 27.1 bits (57), Expect = 3.9
Identities = 12/25 (48%), Positives = 12/25 (48%), Gaps = 2/25 (8%)
Frame = +3
Query: 462 PPRXXP--AXXXPPXPPPPPXXXPP 530
PP P A P PPPPP PP
Sbjct: 224 PPTYTPKQADPLPAPPPPPPPTLPP 248
Score = 26.6 bits (56), Expect = 5.2
Identities = 9/18 (50%), Positives = 10/18 (55%)
Frame = +3
Query: 462 PPRXXPAXXXPPXPPPPP 515
P P+ PP PPPPP
Sbjct: 179 PATSLPSDYNPPPPPPPP 196
Score = 26.6 bits (56), Expect(2) = 0.61
Identities = 11/24 (45%), Positives = 11/24 (45%)
Frame = +2
Query: 455 PPPPPXXAGXLXXPXPPPPPXXXP 526
P P A L P PPPPP P
Sbjct: 225 PTYTPKQADPLPAPPPPPPPTLPP 248
Score = 21.4 bits (43), Expect(2) = 0.61
Identities = 7/11 (63%), Positives = 8/11 (72%)
Frame = +2
Query: 437 PA*XNXPPPPP 469
P+ N PPPPP
Sbjct: 184 PSDYNPPPPPP 194
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 29.5 bits (63), Expect = 0.74
Identities = 19/63 (30%), Positives = 21/63 (33%), Gaps = 2/63 (3%)
Frame = +3
Query: 450 IXXPP--PRXXPAXXXPPXPPPPPXXXPPAPXVPXGXGXXXXXPXXGGXTXXXPPXXAGG 623
+ PP P P P PP P P AP +P P PP A
Sbjct: 417 VPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPP-AAPA 475
Query: 624 PPP 632
PPP
Sbjct: 476 PPP 478
Score = 27.9 bits (59), Expect = 2.3
Identities = 14/33 (42%), Positives = 14/33 (42%), Gaps = 1/33 (3%)
Frame = +3
Query: 450 IXXPPPRXXPAXXX-PPXPPPPPXXXPPAPXVP 545
I P P PA PP P PP PAP P
Sbjct: 455 IAPPLPAGMPAAPPLPPAAPAPPPAPAPAPAAP 487
Score = 25.8 bits (54), Expect = 9.1
Identities = 15/56 (26%), Positives = 17/56 (30%)
Frame = +3
Query: 462 PPRXXPAXXXPPXPPPPPXXXPPAPXVPXGXGXXXXXPXXGGXTXXXPPXXAGGPP 629
PP P P PP P PP+ +P G P P PP
Sbjct: 415 PPVPTPPSLPPSAPPSLPPSAPPS--LPMGAPAAPPLPPSAPIAPPLPAGMPAAPP 468
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 27.5 bits (58), Expect = 3.0
Identities = 14/29 (48%), Positives = 15/29 (51%)
Frame = -2
Query: 544 GTXGAGGXXXGGGGGXGGXXXAGXXRGGG 458
G+ G G GG GG GG G RGGG
Sbjct: 13 GSRGGRGGFNGGRGGFGGGR--GGARGGG 39
Score = 26.2 bits (55), Expect = 6.9
Identities = 13/26 (50%), Positives = 13/26 (50%), Gaps = 1/26 (3%)
Frame = -2
Query: 535 GAGGXXXGG-GGGXGGXXXAGXXRGG 461
G GG GG GG GG G RGG
Sbjct: 31 GRGGARGGGRGGARGGRGGRGGARGG 56
Score = 26.2 bits (55), Expect = 6.9
Identities = 12/28 (42%), Positives = 13/28 (46%)
Frame = -2
Query: 544 GTXGAGGXXXGGGGGXGGXXXAGXXRGG 461
G GA G G GG GG + RGG
Sbjct: 39 GRGGARGGRGGRGGARGGRGGSSGGRGG 66
>SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex
protein Gar1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 194
Score = 27.5 bits (58), Expect = 3.0
Identities = 14/30 (46%), Positives = 14/30 (46%)
Frame = -2
Query: 550 PXGTXGAGGXXXGGGGGXGGXXXAGXXRGG 461
P G G GG G GG GG G RGG
Sbjct: 134 PAGRGGRGGFRGGRGGSRGG--FGGNSRGG 161
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 27.1 bits (57), Expect = 3.9
Identities = 13/29 (44%), Positives = 13/29 (44%)
Frame = -2
Query: 544 GTXGAGGXXXGGGGGXGGXXXAGXXRGGG 458
G G GG G GGG GG GGG
Sbjct: 236 GPGGFGGGPGGFGGGLGGFGGGPGGFGGG 264
Score = 25.8 bits (54), Expect = 9.1
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = -2
Query: 544 GTXGAGGXXXGGGGGXGGXXXAGXXRGG 461
G G GG G GGG GG GG
Sbjct: 243 GPGGFGGGLGGFGGGPGGFGGGPGGHGG 270
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 27.1 bits (57), Expect = 3.9
Identities = 18/58 (31%), Positives = 18/58 (31%)
Frame = +3
Query: 459 PPPRXXPAXXXPPXPPPPPXXXPPAPXVPXGXGXXXXXPXXGGXTXXXPPXXAGGPPP 632
PPP A P PP P PP P P P PP PPP
Sbjct: 150 PPPSPASAPPIPSKAPPIPSSLPP-PAQP--AAPVKSPPSAPSLPSAVPPMPPKVPPP 204
>SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein
Cap1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 551
Score = 23.4 bits (48), Expect(2) = 5.2
Identities = 8/12 (66%), Positives = 8/12 (66%)
Frame = +2
Query: 479 GXLXXPXPPPPP 514
G L P PPPPP
Sbjct: 303 GGLPPPPPPPPP 314
Score = 21.4 bits (43), Expect(2) = 5.2
Identities = 7/10 (70%), Positives = 7/10 (70%)
Frame = +2
Query: 500 PPPPPXXXPS 529
PPPPP PS
Sbjct: 306 PPPPPPPPPS 315
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 26.2 bits (55), Expect = 6.9
Identities = 11/43 (25%), Positives = 15/43 (34%)
Frame = +3
Query: 417 GGXGRXXLXXXIXXPPPRXXPAXXXPPXPPPPPXXXPPAPXVP 545
G + + PP P PPPPP +P +P
Sbjct: 716 GAVNSPAIKPQVTPAPPTPAPTPAVKHHPPPPPVRSSISPSMP 758
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,545,269
Number of Sequences: 5004
Number of extensions: 18603
Number of successful extensions: 286
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 51
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 161
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 493304942
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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