Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP03_F_A02
(853 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g56720.1 68418.m07079 malate dehydrogenase, cytosolic, putati... 87 1e-17
At1g04410.1 68414.m00432 malate dehydrogenase, cytosolic, putati... 86 2e-17
At5g43330.1 68418.m05296 malate dehydrogenase, cytosolic, putati... 84 1e-16
At5g58330.2 68418.m07304 malate dehydrogenase [NADP], chloroplas... 67 2e-11
At5g58330.1 68418.m07303 malate dehydrogenase [NADP], chloroplas... 67 2e-11
At5g58330.3 68418.m07302 malate dehydrogenase [NADP], chloroplas... 54 1e-07
At2g05440.2 68415.m00575 glycine-rich protein 30 1.7
At2g05440.1 68415.m00574 glycine-rich protein 30 1.7
At5g25350.1 68418.m03007 F-box family protein contains Pfam PF00... 29 3.9
At4g37580.1 68417.m05319 N-acetyltransferase, putative / hookles... 29 5.2
At2g33410.1 68415.m04095 heterogeneous nuclear ribonucleoprotein... 28 9.1
At2g15780.1 68415.m01809 glycine-rich protein similar to Blue co... 28 9.1
>At5g56720.1 68418.m07079 malate dehydrogenase, cytosolic, putative
similar to cytosolic malate dehydrogenase from
Mesembryanthemum crystallinum [SP|O24047], Medicago
sativa [SP|O48905], Prunus persica [GI:15982948];
contains InterPro entry IPR001236: Lactate/malate
dehydrogenase
Length = 339
Score = 87.4 bits (207), Expect = 1e-17
Identities = 42/77 (54%), Positives = 51/77 (66%)
Frame = +1
Query: 193 EPIRVVVTGAAGQIAYSLLYQIASGAVFGPQQPVFLHLLDIAPMMGVLEGVVMELADCAL 372
+PIRV++TGAAG I Y++ IA G + GP QP+ LHLLDI P LE V MEL D A
Sbjct: 10 DPIRVLITGAAGNIGYAIAPMIARGIMLGPDQPMILHLLDIEPASSSLEAVKMELQDSAF 69
Query: 373 PLLAGVLPTANPEEASR 423
PLL GV+ T N EA +
Sbjct: 70 PLLKGVIATTNVVEACK 86
Score = 39.5 bits (88), Expect = 0.003
Identities = 25/80 (31%), Positives = 40/80 (50%)
Frame = +2
Query: 404 ILKKLQDVAAAFLXGAMPRKEGMERKDLLAANVRXFKXKARLWTKRLVXM*RSLVVGHPT 583
+++ +DV + G PR GMERKD+++ NV +K +A + + LVV +P
Sbjct: 81 VVEACKDVNIVIMIGGFPRIAGMERKDVMSKNVVIYKAQASALERYASDDCKVLVVANPA 140
Query: 584 PIPNAPICSKICPPXFPKXN 643
NA I + P P+ N
Sbjct: 141 N-TNALILKEFA-PSIPEEN 158
>At1g04410.1 68414.m00432 malate dehydrogenase, cytosolic, putative
strong similarity to malate dehydrogenase from
Mesembryanthemum crystallinum [SP|O24047], Medicago
sativa [SP|O48905], Prunus persica [GI:15982948];
contains InterPro entry IPR001236: Lactate/malate
dehydrogenase
Length = 332
Score = 86.2 bits (204), Expect = 2e-17
Identities = 42/74 (56%), Positives = 50/74 (67%)
Frame = +1
Query: 193 EPIRVVVTGAAGQIAYSLLYQIASGAVFGPQQPVFLHLLDIAPMMGVLEGVVMELADCAL 372
EP+RV+VTGAAGQI Y+L+ IA G + G QPV LH+LDI P L GV MEL D A
Sbjct: 4 EPVRVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPPAAEALNGVKMELIDAAF 63
Query: 373 PLLAGVLPTANPEE 414
PLL GV+ T + E
Sbjct: 64 PLLKGVVATTDAVE 77
Score = 46.8 bits (106), Expect = 2e-05
Identities = 28/73 (38%), Positives = 38/73 (52%)
Frame = +2
Query: 425 VAAAFLXGAMPRKEGMERKDLLAANVRXFKXKARLWTKRLVXM*RSLVVGHPTPIPNAPI 604
V A + G PRKEGMERKD+++ NV +K +A K + LVV +P NA I
Sbjct: 82 VNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQAAALEKHAAPNCKVLVVANPAN-TNALI 140
Query: 605 CSKICPPXFPKXN 643
+ P P+ N
Sbjct: 141 LKEFA-PSIPEKN 152
>At5g43330.1 68418.m05296 malate dehydrogenase, cytosolic, putative
strong similarity to cytosolic malate dehydrogenase (EC
1.1.1.37) SP|O24047 {Mesembryanthemum crystallinum},
SP|O48905 {Medicago sativa}, [Prunus persica]
GI:15982948; contains InterPro entry IPR001236:
Lactate/malate dehydrogenase
Length = 332
Score = 83.8 bits (198), Expect = 1e-16
Identities = 42/75 (56%), Positives = 50/75 (66%)
Frame = +1
Query: 193 EPIRVVVTGAAGQIAYSLLYQIASGAVFGPQQPVFLHLLDIAPMMGVLEGVVMELADCAL 372
EP+RV+VTGAAGQI Y+L+ IA G + G QPV LH+LDI L GV MEL D A
Sbjct: 4 EPVRVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPFAAEALNGVKMELVDAAF 63
Query: 373 PLLAGVLPTANPEEA 417
PLL GV+ T + EA
Sbjct: 64 PLLKGVVATTDAVEA 78
Score = 46.8 bits (106), Expect = 2e-05
Identities = 28/73 (38%), Positives = 38/73 (52%)
Frame = +2
Query: 425 VAAAFLXGAMPRKEGMERKDLLAANVRXFKXKARLWTKRLVXM*RSLVVGHPTPIPNAPI 604
V A + G PRKEGMERKD+++ NV +K +A K + LVV +P NA I
Sbjct: 82 VNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKHAAPNCKVLVVANPAN-TNALI 140
Query: 605 CSKICPPXFPKXN 643
+ P P+ N
Sbjct: 141 LKEFA-PSIPEKN 152
>At5g58330.2 68418.m07304 malate dehydrogenase [NADP], chloroplast,
putative strong similiarity to chloroplast
NADP-dependent malate dehydrogenase (EC 1.1.1.82)
SP|O48902 {Medicago sativa}, SP|P21528 {Pisum sativum},
SP|Q05145 {Mesembryanthemum crystallinum}, SP|P46489
{Flaveria bidentis}, [Flaveria trinervia] GI:726334,
SP|P17606I {Sorghum bicolor}; contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 442
Score = 66.9 bits (156), Expect = 2e-11
Identities = 35/72 (48%), Positives = 44/72 (61%)
Frame = +1
Query: 199 IRVVVTGAAGQIAYSLLYQIASGAVFGPQQPVFLHLLDIAPMMGVLEGVVMELADCALPL 378
I + V+GAAG I+ LL+++ASG VFGP QP+ L LL + LEGV MEL D PL
Sbjct: 99 INIAVSGAAGMISNHLLFKLASGEVFGPDQPIALKLLGSERSIQALEGVAMELEDSLFPL 158
Query: 379 LAGVLPTANPEE 414
L V +P E
Sbjct: 159 LREVDIGTDPNE 170
Score = 48.8 bits (111), Expect = 5e-06
Identities = 33/76 (43%), Positives = 38/76 (50%)
Frame = +2
Query: 419 QDVAAAFLXGAMPRKEGMERKDLLAANVRXFKXKARLWTKRLVXM*RSLVVGHPTPIPNA 598
QDV A L GA PR GMER DLL N + F + + K + LVVG+P NA
Sbjct: 173 QDVEWAILIGAKPRGPGMERADLLDINGQIFAEQGKALNKAASPNVKVLVVGNPCN-TNA 231
Query: 599 PICSKICPPXFPKXNF 646
IC K P P NF
Sbjct: 232 LICLKNA-PNIPAKNF 246
>At5g58330.1 68418.m07303 malate dehydrogenase [NADP], chloroplast,
putative strong similiarity to chloroplast
NADP-dependent malate dehydrogenase (EC 1.1.1.82)
SP|O48902 {Medicago sativa}, SP|P21528 {Pisum sativum},
SP|Q05145 {Mesembryanthemum crystallinum}, SP|P46489
{Flaveria bidentis}, [Flaveria trinervia] GI:726334,
SP|P17606I {Sorghum bicolor}; contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 443
Score = 66.9 bits (156), Expect = 2e-11
Identities = 35/72 (48%), Positives = 44/72 (61%)
Frame = +1
Query: 199 IRVVVTGAAGQIAYSLLYQIASGAVFGPQQPVFLHLLDIAPMMGVLEGVVMELADCALPL 378
I + V+GAAG I+ LL+++ASG VFGP QP+ L LL + LEGV MEL D PL
Sbjct: 100 INIAVSGAAGMISNHLLFKLASGEVFGPDQPIALKLLGSERSIQALEGVAMELEDSLFPL 159
Query: 379 LAGVLPTANPEE 414
L V +P E
Sbjct: 160 LREVDIGTDPNE 171
Score = 48.8 bits (111), Expect = 5e-06
Identities = 33/76 (43%), Positives = 38/76 (50%)
Frame = +2
Query: 419 QDVAAAFLXGAMPRKEGMERKDLLAANVRXFKXKARLWTKRLVXM*RSLVVGHPTPIPNA 598
QDV A L GA PR GMER DLL N + F + + K + LVVG+P NA
Sbjct: 174 QDVEWAILIGAKPRGPGMERADLLDINGQIFAEQGKALNKAASPNVKVLVVGNPCN-TNA 232
Query: 599 PICSKICPPXFPKXNF 646
IC K P P NF
Sbjct: 233 LICLKNA-PNIPAKNF 247
>At5g58330.3 68418.m07302 malate dehydrogenase [NADP], chloroplast,
putative strong similiarity to chloroplast
NADP-dependent malate dehydrogenase (EC 1.1.1.82)
SP|O48902 {Medicago sativa}, SP|P21528 {Pisum sativum},
SP|Q05145 {Mesembryanthemum crystallinum}, SP|P46489
{Flaveria bidentis}, [Flaveria trinervia] GI:726334,
SP|P17606I {Sorghum bicolor}; contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 334
Score = 54.0 bits (124), Expect = 1e-07
Identities = 29/61 (47%), Positives = 36/61 (59%)
Frame = +1
Query: 232 IAYSLLYQIASGAVFGPQQPVFLHLLDIAPMMGVLEGVVMELADCALPLLAGVLPTANPE 411
I+ LL+++ASG VFGP QP+ L LL + LEGV MEL D PLL V +P
Sbjct: 2 ISNHLLFKLASGEVFGPDQPIALKLLGSERSIQALEGVAMELEDSLFPLLREVDIGTDPN 61
Query: 412 E 414
E
Sbjct: 62 E 62
Score = 48.8 bits (111), Expect = 5e-06
Identities = 33/76 (43%), Positives = 38/76 (50%)
Frame = +2
Query: 419 QDVAAAFLXGAMPRKEGMERKDLLAANVRXFKXKARLWTKRLVXM*RSLVVGHPTPIPNA 598
QDV A L GA PR GMER DLL N + F + + K + LVVG+P NA
Sbjct: 65 QDVEWAILIGAKPRGPGMERADLLDINGQIFAEQGKALNKAASPNVKVLVVGNPCN-TNA 123
Query: 599 PICSKICPPXFPKXNF 646
IC K P P NF
Sbjct: 124 LICLKNA-PNIPAKNF 138
>At2g05440.2 68415.m00575 glycine-rich protein
Length = 154
Score = 30.3 bits (65), Expect = 1.7
Identities = 23/72 (31%), Positives = 24/72 (33%)
Frame = -2
Query: 831 PNQXGXGHXGXHSGXXGXGGXGXPPGXITXXKHSLXGXXPIXSRXSWGXGPCFGSRXGSW 652
P G GH G H G G GG G G H L G G G G
Sbjct: 40 PEGYGGGHGG-HGGHGGGGGHGHG-GHNGGGGHGLDGYGGGGGHYGGGGGHYGGGGGHYG 97
Query: 651 PGKFXFGNXGGH 616
G +G GGH
Sbjct: 98 GGGGHYGGGGGH 109
>At2g05440.1 68415.m00574 glycine-rich protein
Length = 127
Score = 30.3 bits (65), Expect = 1.7
Identities = 23/72 (31%), Positives = 24/72 (33%)
Frame = -2
Query: 831 PNQXGXGHXGXHSGXXGXGGXGXPPGXITXXKHSLXGXXPIXSRXSWGXGPCFGSRXGSW 652
P G GH G H G G GG G G H L G G G G
Sbjct: 40 PEGYGGGHGG-HGGHGGGGGHGHG-GHNGGGGHGLDGYGGGGGHYGGGGGHYGGGGGHYG 97
Query: 651 PGKFXFGNXGGH 616
G +G GGH
Sbjct: 98 GGGGGYGGGGGH 109
>At5g25350.1 68418.m03007 F-box family protein contains Pfam
PF00646: F-box domain and Pfam PF00560: Leucine Rich
Repeat (6 copies); similar to F-box protein FBL6
(GI:4432860) [Homo sapiens]
Length = 623
Score = 29.1 bits (62), Expect = 3.9
Identities = 18/54 (33%), Positives = 25/54 (46%)
Frame = +3
Query: 222 CRTNCILTSLSNCVWSSFWTSTTCLPPPS*YCAYDGCT*RCCHGVGRLCSATFG 383
C + SL+NC+ S + S + LP PS + + RCC G G A G
Sbjct: 402 CGSKLKAFSLANCLGISDFNSESSLPSPS-CSSLRSLSIRCCPGFGDASLAFLG 454
>At4g37580.1 68417.m05319 N-acetyltransferase, putative / hookless1
(HLS1) contains Pfam profile PF00583: acetyltransferase,
GNAT family; identical to cDNA putative
N-acetyltransferase hookless1 (HLS1) GI:1277089
Length = 403
Score = 28.7 bits (61), Expect = 5.2
Identities = 10/17 (58%), Positives = 11/17 (64%)
Frame = -2
Query: 684 GPCFGSRXGSWPGKFXF 634
G C+GS GSWPG F
Sbjct: 229 GSCYGSGSGSWPGSAKF 245
>At2g33410.1 68415.m04095 heterogeneous nuclear ribonucleoprotein,
putative / hnRNP, putative
Length = 404
Score = 27.9 bits (59), Expect = 9.1
Identities = 22/90 (24%), Positives = 28/90 (31%)
Frame = -2
Query: 843 WGGPPNQXGXGHXGXHSGXXGXGGXGXPPGXITXXKHSLXGXXPIXSRXSWGXGPCFGSR 664
+GG G G+ G G GG G P G R SWG G
Sbjct: 241 YGGSGYGTGYGYGSNGVGYGGFGGYGNPAGAPYGNPSVPGAGFGSGPRSSWGAQAPSGYG 300
Query: 663 XGSWPGKFXFGNXGGHILEHIGAFGIGVGW 574
+ +G GG +G G G+
Sbjct: 301 NVGYGNAAPWGGSGGPGSAVMGQAGASAGY 330
>At2g15780.1 68415.m01809 glycine-rich protein similar to Blue
copper protein precursor (SP:Q41001) {Pisum sativum};
contains a Pfam PF02298: Plastocyanin-like domain
related to blue copper-binding protein; contains a
domain related to blue copper-binding protein
Length = 257
Score = 27.9 bits (59), Expect = 9.1
Identities = 11/23 (47%), Positives = 13/23 (56%)
Frame = -1
Query: 634 WEXGGAYFGTYWXIWYWGGVANN 566
W G + G+ W W WGGV NN
Sbjct: 74 WGFGSSRNGSGWG-WGWGGVPNN 95
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,792,977
Number of Sequences: 28952
Number of extensions: 343118
Number of successful extensions: 942
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 873
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 935
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1980143200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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