BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= MFBP02_F_P21 (914 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value DQ384991-1|ABD51779.1| 94|Apis mellifera allergen Api m 6 vari... 37 2e-04 DQ384990-1|ABD51778.1| 92|Apis mellifera allergen Api m 6 vari... 37 2e-04 >DQ384991-1|ABD51779.1| 94|Apis mellifera allergen Api m 6 variant 2 precursor protein. Length = 94 Score = 37.1 bits (82), Expect = 2e-04 Identities = 13/25 (52%), Positives = 15/25 (60%) Frame = +3 Query: 642 CQAGCVCKEGYLKXDXGTCVPRENC 716 C GCVC+ GYL+ CVPR C Sbjct: 67 CAPGCVCRLGYLRNKKKVCVPRSKC 91 Score = 35.5 bits (78), Expect = 7e-04 Identities = 21/61 (34%), Positives = 26/61 (42%) Frame = +2 Query: 137 CSENEIYVXCVXAHCGPRTCSEXXLPMPCPLVRXXYCXAGCLCKXGXLKXXSGXCVAREN 316 C NEI+ C C R C +P PL C GC+C+ G L+ CV R Sbjct: 37 CPSNEIFSRC-DGRC-QRFCPNV---VPKPLC-IKICAPGCVCRLGYLRNKKKVCVPRSK 90 Query: 317 C 319 C Sbjct: 91 C 91 Score = 35.5 bits (78), Expect = 7e-04 Identities = 19/58 (32%), Positives = 26/58 (44%) Frame = +2 Query: 335 CSENEIYVKCVXAXCXPMTCSKKXGPKICPLVEXXSCKAGCVCKXGYLKDDSGKCVXR 508 C NEI+ +C C C +C + C GCVC+ GYL++ CV R Sbjct: 37 CPSNEIFSRC-DGRCQRF-CPNVVPKPLCIKI----CAPGCVCRLGYLRNKKKVCVPR 88 >DQ384990-1|ABD51778.1| 92|Apis mellifera allergen Api m 6 variant 1 precursor protein. Length = 92 Score = 37.1 bits (82), Expect = 2e-04 Identities = 13/25 (52%), Positives = 15/25 (60%) Frame = +3 Query: 642 CQAGCVCKEGYLKXDXGTCVPRENC 716 C GCVC+ GYL+ CVPR C Sbjct: 67 CAPGCVCRLGYLRNKKKVCVPRSKC 91 Score = 35.5 bits (78), Expect = 7e-04 Identities = 21/61 (34%), Positives = 26/61 (42%) Frame = +2 Query: 137 CSENEIYVXCVXAHCGPRTCSEXXLPMPCPLVRXXYCXAGCLCKXGXLKXXSGXCVAREN 316 C NEI+ C C R C +P PL C GC+C+ G L+ CV R Sbjct: 37 CPSNEIFSRC-DGRC-QRFCPNV---VPKPLC-IKICAPGCVCRLGYLRNKKKVCVPRSK 90 Query: 317 C 319 C Sbjct: 91 C 91 Score = 35.5 bits (78), Expect = 7e-04 Identities = 19/58 (32%), Positives = 26/58 (44%) Frame = +2 Query: 335 CSENEIYVKCVXAXCXPMTCSKKXGPKICPLVEXXSCKAGCVCKXGYLKDDSGKCVXR 508 C NEI+ +C C C +C + C GCVC+ GYL++ CV R Sbjct: 37 CPSNEIFSRC-DGRCQRF-CPNVVPKPLCIKI----CAPGCVCRLGYLRNKKKVCVPR 88 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 173,048 Number of Sequences: 438 Number of extensions: 2843 Number of successful extensions: 50 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 46 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 50 length of database: 146,343 effective HSP length: 58 effective length of database: 120,939 effective search space used: 29750994 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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