Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP02_F_P07
         (900 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   256   2e-69
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   251   1e-67
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   120   4e-28
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    99   7e-22

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  256 bits (628), Expect = 2e-69
 Identities = 118/215 (54%), Positives = 144/215 (66%)
 Frame = +2

Query: 155 MRXCISVHVGXAGVQXGNACWELYCLEHGIQPDGXMPTDKTXXXXXXSFNTFFSETGAGK 334
           MR  IS+HVG AG Q GNACWELYCLEHGIQP+G M  +         F+TFFSETG GK
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60

Query: 335 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEXAAXNYARGXYTIGKEIVDLVLD 514
           +VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKE A+ NYARG YT+GKE+VD V D
Sbjct: 61  YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120

Query: 515 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGXKSKXEFXIYXXXXXXXX 694
           +IR++AD C+GLQGFL+FH            LL+ERL+++Y  KSK +F +Y        
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180

Query: 695 XXXXYXSIXXXXXXXXXSDCAFXVXNESXXDIXRR 799
               Y S+         +DC F V NES  DI RR
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRR 215


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  251 bits (614), Expect = 1e-67
 Identities = 119/220 (54%), Positives = 146/220 (66%), Gaps = 5/220 (2%)
 Frame = +2

Query: 155 MRXCISVHVGXAGVQXGNACWELYCLEHGIQPDGXMPTDKTXXXXXXS-----FNTFFSE 319
           MR  ISVHVG AGVQ GNACWELYCLEHGI PDG  PT+ +      S     F TFFSE
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59

Query: 320 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEXAAXNYARGXYTIGKEIV 499
           TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKE A+ NYARG YT+GKE++
Sbjct: 60  TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119

Query: 500 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGXKSKXEFXIYXXX 679
           D VL+RIR++AD C+GLQGFL+FH            LL+ERL+++YG KS  +F +Y   
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179

Query: 680 XXXXXXXXXYXSIXXXXXXXXXSDCAFXVXNESXXDIXRR 799
                    Y S+         SDC F V NE+  DI RR
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRR 219


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  120 bits (288), Expect = 4e-28
 Identities = 66/212 (31%), Positives = 95/212 (44%)
 Frame = +2

Query: 155 MRXCISVHVGXAGVQXGNACWELYCLEHGIQPDGXMPTDKTXXXXXXSFNTFFSETGAGK 334
           MR  + +  G  G Q G A W     EHG+   G      T        N +F+E   GK
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58

Query: 335 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEXAAXNYARGXYTIGKEIVDLVLD 514
           +VPRAV VDLEP  +D V++G +  LF P+ +I G+  A   +A+G YT G E+ D VLD
Sbjct: 59  YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118

Query: 515 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGXKSKXEFXIYXXXXXXXX 694
            +R+ A+ C  LQGF + H            LL+ ++  +Y  +    F +         
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178

Query: 695 XXXXYXSIXXXXXXXXXSDCAFXVXNESXXDI 790
               Y +          SD  F + NE+   I
Sbjct: 179 VVEPYNATLSMHQLVENSDETFCIDNEALSSI 210


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 99.1 bits (236), Expect = 7e-22
 Identities = 54/173 (31%), Positives = 89/173 (51%), Gaps = 2/173 (1%)
 Frame = +2

Query: 158 RXCISVHVGXAGVQXGNACWELYCLEHGIQPDGXMPTDKTXXXXXXSFNTFFSETGAGKH 337
           R  I++  G  G Q G+  W+  CLEHGI PDG + +  T        + FF ++   ++
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRK--DVFFYQSDDTRY 60

Query: 338 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--XAAXNYARGXYTIGKEIVDLVL 511
           +PRA+ +DLEP VV+ + + TY  L++PE ++  K    A  N+A G Y+  + I + ++
Sbjct: 61  IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119

Query: 512 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGXKSKXEFXIY 670
           D I + AD    L+GF + H             L+ERL+  Y  K    + ++
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVF 172


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,427,827
Number of Sequences: 5004
Number of extensions: 39736
Number of successful extensions: 85
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 81
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 81
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 454497130
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)